LOCUS       NM_003588               5365 bp    mRNA    linear   PRI 14-JUN-2020
DEFINITION  Homo sapiens cullin 4B (CUL4B), transcript variant 1, mRNA.
ACCESSION   NM_003588
VERSION     NM_003588.3
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5365)
  AUTHORS   Zhang H, Wang X, Hu B, Zhang F, Wei H and Li L.
  TITLE     Circular RNA ZFR accelerates non-small cell lung cancer progression
            by acting as a miR-101-3p sponge to enhance CUL4B expression
  JOURNAL   Artif Cells Nanomed Biotechnol 47 (1), 3410-3416 (2019)
   PUBMED   31407591
  REMARK    GeneRIF: circZFR exhibited a carcinogenic role by sponging
            miR-101-3p and regulating CUL4B expression in NSCLC
REFERENCE   2  (bases 1 to 5365)
  AUTHORS   Chen Z, Han Y, Deng C, Chen W, Jin L, Chen H, Wang K, Shen H and
            Qian L.
  TITLE     Inflammation-dependent downregulation of miR-194-5p contributes to
            human intervertebral disc degeneration by targeting CUL4A and CUL4B
  JOURNAL   J. Cell. Physiol. 234 (11), 19977-19989 (2019)
   PUBMED   30945295
  REMARK    GeneRIF: miR-194-5p, was significantly downregulated in
            intervertebral disc degeneration samples and could bind to the
            three prime untranslated regions (3'-UTRs) of both CUL4A and CUL4B,
            thereby downregulating their expression.
REFERENCE   3  (bases 1 to 5365)
  AUTHORS   Jiao M, Qi M, Zhang F, Hu J, Feng T, Zhao M, Li X, Liu H, Teng W,
            Zhang J, Liu Z, Zhang L, Wu Z and Han B.
  TITLE     CUL4B regulates cancer stem-like traits of prostate cancer cells by
            targeting BMI1 via miR200b/c
  JOURNAL   Prostate 79 (11), 1294-1303 (2019)
   PUBMED   31111526
  REMARK    GeneRIF: CUL4B regulates cancer stem-like traits of prostate cancer
            cells by targeting BMI1 via miR200b/c, which might give novel
            insight into how CUL4B promotes prostate cancer progression through
            regulating cancer stem-like traits.
REFERENCE   4  (bases 1 to 5365)
  AUTHORS   Ohta E, Itoh M, Ueda M, Hida Y, Wang MX, Hayakawa-Ogura M, Li S,
            Nishida E, Ohta K, Tana, Islam S, Nakagawa K, Sunayama T, Chen H,
            Hirata S, Endo M, Ohno Y and Nakagawa T.
  TITLE     Cullin-4B E3 ubiquitin ligase mediates Apaf-1 ubiquitination to
            regulate caspase-9 activity
  JOURNAL   PLoS ONE 14 (7), e0219782 (2019)
   PUBMED   31329620
  REMARK    GeneRIF: These results show that ubiquitinated Apaf-1 may activate
            caspase-9 under conditions of proteasome impairment.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5365)
  AUTHORS   Wang Y and Yue D.
  TITLE     CUL4B promotes aggressive phenotypes of HNSCC via the activation of
            the Wnt/beta-catenin signaling pathway
  JOURNAL   Cancer Med 8 (5), 2278-2287 (2019)
   PUBMED   30883036
  REMARK    GeneRIF: Results demonstrate a key contribution of CUL4B
            overexpression in the malignant behavior of head and neck squamous
            cell carcinoma (HNSCC) cells, at least in part through the
            stimulation of angiogenesis and the activation of the
            Wnt/beta-catenin signaling pathway.
REFERENCE   6  (bases 1 to 5365)
  AUTHORS   Jones EA, Clement-Jones M and Wilson DI.
  TITLE     JAGGED1 expression in human embryos: correlation with the Alagille
            syndrome phenotype
  JOURNAL   J. Med. Genet. 37 (9), 658-662 (2000)
   PUBMED   10978356
REFERENCE   7  (bases 1 to 5365)
  AUTHORS   Hori T, Osaka F, Chiba T, Miyamoto C, Okabayashi K, Shimbara N,
            Kato S and Tanaka K.
  TITLE     Covalent modification of all members of human cullin family
            proteins by NEDD8
  JOURNAL   Oncogene 18 (48), 6829-6834 (1999)
   PUBMED   10597293
REFERENCE   8  (bases 1 to 5365)
  AUTHORS   Wada H, Yeh ET and Kamitani T.
  TITLE     Identification of NEDD8-conjugation site in human cullin-2
  JOURNAL   Biochem. Biophys. Res. Commun. 257 (1), 100-105 (1999)
   PUBMED   10092517
REFERENCE   9  (bases 1 to 5365)
  AUTHORS   Ohta T, Michel JJ, Schottelius AJ and Xiong Y.
  TITLE     ROC1, a homolog of APC11, represents a family of cullin partners
            with an associated ubiquitin ligase activity
  JOURNAL   Mol. Cell 3 (4), 535-541 (1999)
   PUBMED   10230407
REFERENCE   10 (bases 1 to 5365)
  AUTHORS   Kipreos ET, Lander LE, Wing JP, He WW and Hedgecock EM.
  TITLE     cul-1 is required for cell cycle exit in C. elegans and identifies
            a novel gene family
  JOURNAL   Cell 85 (6), 829-839 (1996)
   PUBMED   8681378
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB092748.1, BC036216.1,
            AC002476.1 and CV573599.1.
            This sequence is a reference standard in the RefSeqGene project.
            On Jan 5, 2007 this sequence version replaced NM_003588.2.
            
            Summary: This gene is a member of the cullin family. The encoded
            protein forms a complex that functions as an E3 ubiquitin ligase
            and catalyzes the polyubiquitination of specific protein substrates
            in the cell. The protein interacts with a ring finger protein, and
            is required for the proteolysis of several regulators of DNA
            replication including chromatin licensing and DNA replication
            factor 1 and cyclin E. Multiple transcript variants encoding
            different isoforms have been found for this gene. [provided by
            RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC036216.1, AK315037.1 [ECO:0000332]
            RNAseq introns              :: mixed/partial sample support
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000350]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-35                DB092748.1         1-35
            36-2615             BC036216.1         1-2580
            2616-2616           AC002476.1         109703-109703       c
            2617-3299           BC036216.1         2582-3264
            3300-5000           AC002476.1         98778-100478        c
            5001-5365           CV573599.1         1-365
FEATURES             Location/Qualifiers
     source          1..5365
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq24"
     gene            1..5365
                     /gene="CUL4B"
                     /gene_synonym="CUL-4B; MRXHF2; MRXS15; MRXSC; SFM2"
                     /note="cullin 4B"
                     /db_xref="GeneID:8450"
                     /db_xref="HGNC:HGNC:2555"
                     /db_xref="MIM:300304"
     exon            1..424
                     /gene="CUL4B"
                     /gene_synonym="CUL-4B; MRXHF2; MRXS15; MRXSC; SFM2"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    420..422
                     /gene="CUL4B"
                     /gene_synonym="CUL-4B; MRXHF2; MRXS15; MRXSC; SFM2"
                     /note="upstream in-frame stop codon"
     exon            425..504
                     /gene="CUL4B"
                     /gene_synonym="CUL-4B; MRXHF2; MRXS15; MRXSC; SFM2"
                     /inference="alignment:Splign:2.1.0"
     CDS             438..3179
                     /gene="CUL4B"
                     /gene_synonym="CUL-4B; MRXHF2; MRXS15; MRXSC; SFM2"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /codon_start=1
                     /product="cullin-4B isoform 1"
                     /protein_id="NP_003579.3"
                     /db_xref="CCDS:CCDS35379.1"
                     /db_xref="GeneID:8450"
                     /db_xref="HGNC:HGNC:2555"
                     /db_xref="MIM:300304"
                     /translation="MMSQSSGSGDGNDDEATTSKDGGFSSPSPSAAAAAQEVRSATDG
                     NTSTTPPTSAKKRKLNSSSSSSSNSSNEREDFDSTSSSSSTPPLQPRDSASPSTSSFC
                     LGVSVAASSHVPIQKKLRFEDTLEFVGFDAKMAEESSSSSSSSSPTAATSQQQQLKNK
                     SILISSVASVHHANGLAKSSTTVSSFANSKPGSAKKLVIKNFKDKPKLPENYTDETWQ
                     KLKEAVEAIQNSTSIKYNLEELYQAVENLCSYKISANLYKQLRQICEDHIKAQIHQFR
                     EDSLDSVLFLKKIDRCWQNHCRQMIMIRSIFLFLDRTYVLQNSMLPSIWDMGLELFRA
                     HIISDQKVQNKTIDGILLLIERERNGEAIDRSLLRSLLSMLSDLQIYQDSFEQRFLEE
                     TNRLYAAEGQKLMQEREVPEYLHHVNKRLEEEADRLITYLDQTTQKSLIATVEKQLLG
                     EHLTAILQKGLNNLLDENRIQDLSLLYQLFSRVRGGVQVLLQQWIEYIKAFGSTIVIN
                     PEKDKTMVQELLDFKDKVDHIIDICFLKNEKFINAMKEAFETFINKRPNKPAELIAKY
                     VDSKLRAGNKEATDEELEKMLDKIMIIFRFIYGKDVFEAFYKKDLAKRLLVGKSASVD
                     AEKSMLSKLKHECGAAFTSKLEGMFKDMELSKDIMIQFKQYMQNQNVPGNIELTVNIL
                     TMGYWPTYVPMEVHLPPEMVKLQEIFKTFYLGKHSGRKLQWQSTLGHCVLKAEFKEGK
                     KELQVSLFQTLVLLMFNEGEEFSLEEIKQATGIEDGELRRTLQSLACGKARVLAKNPK
                     GKDIEDGDKFICNDDFKHKLFRIKINQIQMKETVEEQASTTERVFQDRQYQIDAAIVR
                     IMKMRKTLSHNLLVSEVYNQLKFPVKPADLKKRIESLIDRDYMERDKENPNQYNYIA"
     misc_feature    438..440
                     /gene="CUL4B"
                     /gene_synonym="CUL-4B; MRXHF2; MRXS15; MRXSC; SFM2"
                     /note="N-acetylmethionine.
                     /evidence=ECO:0000244|PubMed:20068231; propagated from
                     UniProtKB/Swiss-Prot (Q13620.4); acetylation site"
     misc_feature    459..461
                     /gene="CUL4B"
                     /gene_synonym="CUL-4B; MRXHF2; MRXS15; MRXSC; SFM2"
                     /note="Phosphoserine.
                     /evidence=ECO:0000244|PubMed:20068231; propagated from
                     UniProtKB/Swiss-Prot (Q13620.4); phosphorylation site"
     misc_feature    465..467
                     /gene="CUL4B"
                     /gene_synonym="CUL-4B; MRXHF2; MRXS15; MRXSC; SFM2"
                     /note="Phosphoserine.
                     /evidence=ECO:0000244|PubMed:20068231; propagated from
                     UniProtKB/Swiss-Prot (Q13620.4); phosphorylation site"
     misc_feature    582..584
                     /gene="CUL4B"
                     /gene_synonym="CUL-4B; MRXHF2; MRXS15; MRXSC; SFM2"
                     /note="Phosphothreonine.
                     /evidence=ECO:0000244|PubMed:18669648,
                     ECO:0000244|PubMed:19690332, ECO:0000244|PubMed:23186163;
                     propagated from UniProtKB/Swiss-Prot (Q13620.4);
                     phosphorylation site"
     misc_feature    594..596
                     /gene="CUL4B"
                     /gene_synonym="CUL-4B; MRXHF2; MRXS15; MRXSC; SFM2"
                     /note="Phosphoserine.
                     /evidence=ECO:0000244|PubMed:18669648; propagated from
                     UniProtKB/Swiss-Prot (Q13620.4); phosphorylation site"
     misc_feature    600..611
                     /gene="CUL4B"
                     /gene_synonym="CUL-4B; MRXHF2; MRXS15; MRXSC; SFM2"
                     /note="propagated from UniProtKB/Swiss-Prot (Q13620.4);
                     Region: Nuclear localization signal.
                     /evidence=ECO:0000269|PubMed:19801544"
     misc_feature    735..737
                     /gene="CUL4B"
                     /gene_synonym="CUL-4B; MRXHF2; MRXS15; MRXSC; SFM2"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q13619; propagated from
                     UniProtKB/Swiss-Prot (Q13620.4); phosphorylation site"
     misc_feature    873..875
                     /gene="CUL4B"
                     /gene_synonym="CUL-4B; MRXHF2; MRXS15; MRXSC; SFM2"
                     /note="Phosphoserine.
                     /evidence=ECO:0000244|PubMed:18669648,
                     ECO:0000244|PubMed:19690332; propagated from
                     UniProtKB/Swiss-Prot (Q13620.4); phosphorylation site"
     misc_feature    879..881
                     /gene="CUL4B"
                     /gene_synonym="CUL-4B; MRXHF2; MRXS15; MRXSC; SFM2"
                     /note="Phosphothreonine.
                     /evidence=ECO:0000250|UniProtKB:A2A432; propagated from
                     UniProtKB/Swiss-Prot (Q13620.4); phosphorylation site"
     misc_feature    1014..1016
                     /gene="CUL4B"
                     /gene_synonym="CUL-4B; MRXHF2; MRXS15; MRXSC; SFM2"
                     /note="Phosphoserine.
                     /evidence=ECO:0000244|PubMed:18669648,
                     ECO:0000244|PubMed:23186163; propagated from
                     UniProtKB/Swiss-Prot (Q13620.4); phosphorylation site"
     exon            505..1047
                     /gene="CUL4B"
                     /gene_synonym="CUL-4B; MRXHF2; MRXS15; MRXSC; SFM2"
                     /inference="alignment:Splign:2.1.0"
     exon            1048..1163
                     /gene="CUL4B"
                     /gene_synonym="CUL-4B; MRXHF2; MRXS15; MRXSC; SFM2"
                     /inference="alignment:Splign:2.1.0"
     exon            1164..1267
                     /gene="CUL4B"
                     /gene_synonym="CUL-4B; MRXHF2; MRXS15; MRXSC; SFM2"
                     /inference="alignment:Splign:2.1.0"
     exon            1268..1337
                     /gene="CUL4B"
                     /gene_synonym="CUL-4B; MRXHF2; MRXS15; MRXSC; SFM2"
                     /inference="alignment:Splign:2.1.0"
     exon            1338..1411
                     /gene="CUL4B"
                     /gene_synonym="CUL-4B; MRXHF2; MRXS15; MRXSC; SFM2"
                     /inference="alignment:Splign:2.1.0"
     exon            1412..1574
                     /gene="CUL4B"
                     /gene_synonym="CUL-4B; MRXHF2; MRXS15; MRXSC; SFM2"
                     /inference="alignment:Splign:2.1.0"
     exon            1575..1664
                     /gene="CUL4B"
                     /gene_synonym="CUL-4B; MRXHF2; MRXS15; MRXSC; SFM2"
                     /inference="alignment:Splign:2.1.0"
     exon            1665..1747
                     /gene="CUL4B"
                     /gene_synonym="CUL-4B; MRXHF2; MRXS15; MRXSC; SFM2"
                     /inference="alignment:Splign:2.1.0"
     exon            1748..1815
                     /gene="CUL4B"
                     /gene_synonym="CUL-4B; MRXHF2; MRXS15; MRXSC; SFM2"
                     /inference="alignment:Splign:2.1.0"
     exon            1816..1934
                     /gene="CUL4B"
                     /gene_synonym="CUL-4B; MRXHF2; MRXS15; MRXSC; SFM2"
                     /inference="alignment:Splign:2.1.0"
     exon            1935..2127
                     /gene="CUL4B"
                     /gene_synonym="CUL-4B; MRXHF2; MRXS15; MRXSC; SFM2"
                     /inference="alignment:Splign:2.1.0"
     exon            2128..2232
                     /gene="CUL4B"
                     /gene_synonym="CUL-4B; MRXHF2; MRXS15; MRXSC; SFM2"
                     /inference="alignment:Splign:2.1.0"
     exon            2233..2343
                     /gene="CUL4B"
                     /gene_synonym="CUL-4B; MRXHF2; MRXS15; MRXSC; SFM2"
                     /inference="alignment:Splign:2.1.0"
     exon            2344..2429
                     /gene="CUL4B"
                     /gene_synonym="CUL-4B; MRXHF2; MRXS15; MRXSC; SFM2"
                     /inference="alignment:Splign:2.1.0"
     exon            2430..2537
                     /gene="CUL4B"
                     /gene_synonym="CUL-4B; MRXHF2; MRXS15; MRXSC; SFM2"
                     /inference="alignment:Splign:2.1.0"
     exon            2538..2651
                     /gene="CUL4B"
                     /gene_synonym="CUL-4B; MRXHF2; MRXS15; MRXSC; SFM2"
                     /inference="alignment:Splign:2.1.0"
     exon            2652..2757
                     /gene="CUL4B"
                     /gene_synonym="CUL-4B; MRXHF2; MRXS15; MRXSC; SFM2"
                     /inference="alignment:Splign:2.1.0"
     exon            2758..2930
                     /gene="CUL4B"
                     /gene_synonym="CUL-4B; MRXHF2; MRXS15; MRXSC; SFM2"
                     /inference="alignment:Splign:2.1.0"
     exon            2931..3083
                     /gene="CUL4B"
                     /gene_synonym="CUL-4B; MRXHF2; MRXS15; MRXSC; SFM2"
                     /inference="alignment:Splign:2.1.0"
     exon            3084..5351
                     /gene="CUL4B"
                     /gene_synonym="CUL-4B; MRXHF2; MRXS15; MRXSC; SFM2"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      3246
                     /gene="CUL4B"
                     /gene_synonym="CUL-4B; MRXHF2; MRXS15; MRXSC; SFM2"
     polyA_site      3639
                     /gene="CUL4B"
                     /gene_synonym="CUL-4B; MRXHF2; MRXS15; MRXSC; SFM2"
     regulatory      5306..5311
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CUL4B"
                     /gene_synonym="CUL-4B; MRXHF2; MRXS15; MRXSC; SFM2"
     polyA_site      5328
                     /gene="CUL4B"
                     /gene_synonym="CUL-4B; MRXHF2; MRXS15; MRXSC; SFM2"
     polyA_site      5351
                     /gene="CUL4B"
                     /gene_synonym="CUL-4B; MRXHF2; MRXS15; MRXSC; SFM2"
ORIGIN      
        1 actacccaat tgtgaagaca caatccctcc ccagcagttc agtttagaaa ctgccagtct
       61 tggtatagaa caacctaggc ttatctgttt aagtcccata ggtagtatta ttagagaata
      121 acccaggctt tgggaactgt ataactctga gatcgtttga aattttcgtt ttgtttttct
      181 tttggctcca accaggcctc tgttgttaga ctaatggggt ttcccctgca gagattggtc
      241 agccctgcag tctatcatcc cggaaggtgt taaatacaca tgaaagccag cctcggcggg
      301 cagaacctag cccgccgaaa gggcgggcat tgctaaagtg ttttcaaaag gtcttcccaa
      361 tcctctgctt tagacagctc ggaccttaga tagaagaaca caacggatcc tccagtgact
      421 gacgacccaa aggacggatg atgtcacagt catctggatc aggagatggg aatgatgatg
      481 aggctactac ctctaaagac ggtggttttt cttcccccag tccctcagct gctgctgctg
      541 ctcaggaggt cagatctgcc actgatggta ataccagcac cactccgccc acctctgcca
      601 agaagagaaa gttaaacagc agcagcagta gcagcagtaa cagtagtaac gagagagaag
      661 actttgattc cacctcttcc tcctcttcca ctcctccttt acaacccagg gattcggcat
      721 ccccttcaac ctcgtccttc tgcctggggg tttcagtggc tgcttccagc cacgtaccga
      781 tacagaagaa gctgcgtttt gaagacaccc tggagtttgt agggtttgat gcgaagatgg
      841 ctgaggaatc ctcctcctcc tcctcctcat cttcaccaac tgctgcaaca tctcagcagc
      901 agcaacttaa aaataagagt atattaatct cttctgtggc ttcggtgcat catgcaaacg
      961 gcctagccaa atcttctacc accgtctcta gctttgctaa cagcaaacct ggctctgcta
     1021 agaagttagt gatcaagaac tttaaagata agcctaaatt accagaaaac tacacagatg
     1081 aaacctggca aaaactgaaa gaagcagtgg aagctattca gaatagtact tcaattaagt
     1141 acaatttaga agaactctac caggctgtag aaaatctctg ttcttacaag atttctgcaa
     1201 acttgtacaa acagctgaga cagatctgcg aagatcacat caaagcacag attcatcaat
     1261 tcagagagga ttcattggat agcgttcttt ttttaaagaa gattgataga tgctggcaaa
     1321 accattgcag acaaatgatc atgatcagga gcattttttt gtttctggat agaacttacg
     1381 ttcttcagaa ttcaatgcta ccctccattt gggacatggg actggagtta tttagggctc
     1441 atattataag tgatcagaaa gtgcagaata agacaattga tggcattctt ctcttgattg
     1501 agagggaaag gaatggtgaa gcaattgata gaagtttact tcgaagcctt ttaagcatgc
     1561 tgtctgattt gcaaatttat caagattctt ttgaacaacg atttttggaa gaaactaacc
     1621 ggctctatgc agctgaaggc caaaaattaa tgcaagaaag agaggttcct gaatatctac
     1681 atcatgttaa caaacgtcta gaagaagaag cagacagact tattacttac ttagatcaga
     1741 ccacccagaa gtcattaatt gctactgtag aaaaacaact tctaggtgaa cacttaacag
     1801 caattcttca gaaaggttta aataacctcc ttgatgaaaa ccgaattcaa gatttgtctc
     1861 ttctgtatca gctcttcagt agagttcgag gtggagttca ggttcttttg cagcagtgga
     1921 tcgaatatat caaggcattt ggcagcacta ttgtaattaa tcctgaaaaa gataaaacca
     1981 tggttcaaga attgctggat tttaaagata aggttgacca tataattgat atctgctttc
     2041 tgaagaatga gaaatttatc aatgccatga aagaagcatt tgaaacgttc attaacaaaa
     2101 gaccaaataa accagctgaa cttatagcta agtatgtaga ttcaaaactt cgtgcaggca
     2161 acaaagaagc tacagatgaa gaacttgaga aaatgttgga taaaattatg atcatattta
     2221 gatttatcta tggcaaggat gtttttgagg ccttctataa gaaagattta gccaagcgcc
     2281 tgttagtcgg aaagagtgca tctgtagatg ctgaaaaatc aatgctgtcc aaacttaaac
     2341 atgaatgcgg agctgctttc accagcaaac ttgaaggaat gtttaaagac atggaacttt
     2401 ctaaagacat catgattcag ttcaaacagt atatgcagaa tcagaatgtt ccgggaaata
     2461 ttgagttaac tgtgaatatc ctgacaatgg gctattggcc gacatatgtg cctatggaag
     2521 ttcatttacc accagagatg gtaaaacttc aggagatttt caagacattt tacctaggca
     2581 aacatagtgg caggaaactt cagtggcagt caaccctagg acactgtgtg ttaaaagcag
     2641 aatttaaaga gggtaaaaag gaactccagg tctctctttt tcaaacactg gtgctgctaa
     2701 tgtttaatga gggagaggag ttcagtttag aagagatcaa gcaggcaact ggaatagagg
     2761 atggagagtt aaggagaaca ctgcagtcat tagcctgtgg caaagctaga gttctggcga
     2821 aaaatccaaa gggcaaagac attgaagatg gtgacaagtt catttgtaat gatgatttca
     2881 aacataaact tttcaggata aagatcaatc aaatccagat gaaagaaacg gttgaagaac
     2941 aagcaagcac tacagaaaga gtatttcaag acagacagta tcaaattgat gctgcaattg
     3001 ttcgaattat gaagatgaga aagacactta gccacaatct ccttgtttca gaagtgtaca
     3061 accagttgaa atttccagta aagcctgctg atcttaagaa gagaatagaa tctttaattg
     3121 accgggacta catggaaaga gataaagaaa atccaaacca gtacaactat attgcataga
     3181 atgttggcct tgcagcattt ggtgtcatat gcagtagcca gtggataaac taacctgttg
     3241 attcaatatt atttgactcc ttttttacta ccgatgacca aatgaagcag tgtaattaat
     3301 ggaaatagtt gagtggactt tttctcagtg gttaacatgc ccattttaaa gagtaatact
     3361 tacctttaag aagaatgttg ttgaactctt tgcatgttat ttagtatgat gtgcagaaaa
     3421 cacttaagaa cgtacctggt cttcatgaat tcctctttgg aactgggaaa gagatccctg
     3481 tggctattaa aaagggggga gggttcttac acaccattaa ttatgaagca aaaggtttat
     3541 ttgcttaaaa tgtcatttaa agatacttaa actgcatgca aactttattt actgtacaga
     3601 gttctggatg tatttatcaa atagaaaaac cacccttgac ttggttgttc acccgttttg
     3661 tgatttccct tgaaaagaga aatgaattgt catagctatt gatattttac taggtgatgt
     3721 tctgttacac tgaaagggat gttttttaaa aaaaattatt tggaaacaag ttttcaagta
     3781 gggggacagt tgcttacata atatcttgta tattcacatc ctaaaaattt tcatttatga
     3841 gtatgggatg tgtgtatatg gcttagcccc aagttaactg tgctgattaa caagtactta
     3901 taaaaatagc taaagtagaa aattaatgat cagttgtact agaggaacat catagaagag
     3961 aaaaatccaa ctctttatat taaaaatgaa tacaataaga aaattgcaaa agagaccaaa
     4021 atgaacgtgt tttgtaaatt gttacaggag caaaaacttt gcaactagtt ttcaacatac
     4081 tttagacttt aaatgttgtt tggacagttt tgtcttcaac ttggaccaat tgtagattgt
     4141 tacaggcttc ctaggattta gcattcctat tttctctacc aaagtttttt ttagaagcat
     4201 cccaactctt ctctttgttg ataaataagc ctttcctgtc atcttcattt taacttgcta
     4261 aaaccctaga tacaaaggtc ctccctcatt tttcccacag agcccctgtt tgggaatttg
     4321 agattctgtg tcatgctggt tttcctttca gaattgcttg aaatcgagtt ctcttagcca
     4381 ctggacacct agttttcagg agaatgtccc aagattgttt atatatacaa aatggcaaaa
     4441 gtactgtttt tcgaattgat aattcaagca aaggcttctc tttactgtca ctgatttttt
     4501 tttgttttta ttttgtttcc ttgtttctat ttccttgaag ctttgaaggg aggagaagca
     4561 gaaaattttg tttatgtttg ttaaaaatca gaactactta atgctttttg ccacagcata
     4621 tttccttgcc tgttggagcc gttaggaaga ttacctatgt cctaatcttc aacctgagaa
     4681 gatttttgac tcttgtgtct actgttgatt gtttgcttga gttctcaaag acctgtggcc
     4741 aggtatttta aaaagtcgtc tatttctttc tagttgcatc aacttgcaca ctggtgtcta
     4801 actatatgtg gtattcggaa cttgtaattc ttattgagca gccattgcct atattttctc
     4861 agctcctcac tgatttcttt atatgagctc agctgacaag caaatgcagc tattttgttt
     4921 accctctctt cataccggac catcaacaac agtgttctaa ctcatccctg atggtctgtg
     4981 agatgtagac tattacacaa gattcaagtt actcgtaagc aacttttaga tagagttggt
     5041 cctgttagga ggagactctt gatgtcacct tcagtatctt gaaagcgggt cccctccccg
     5101 aggctcttaa ttctttgaaa acttgatgct gtttcagctg aaaaattagc aagactgtta
     5161 aaaaaaaaaa gttgagggga gctgtttaag aaactgaaaa gtaattgcaa actacattgg
     5221 ataattgtga ctttcagttg ttgttctgta tcagttgaat ttttgtgctc ttttccctgt
     5281 gtacgtggtg gttctatttt tctccaataa aacttttatt taaaaaaagt ttctagagaa
     5341 gatctttaaa aaaaaaaaaa aaaaa
//

