LOCUS pSMALB-ATF4.5 9456 bp DNA circular UNA 13-JUN-2020 DEFINITION Peter van Galen (sequence verified). ACCESSION urn.local...c-c1fm74j VERSION urn.local...c-c1fm74j KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers misc_feature 91..168 /created_by="User" /label="SV40 ori" /note="Geneious type: origin of replication" misc_feature 465..771 /created_by="User" /modified_by="User" /label="F1 phage ori" /note="Geneious type: origin of replication" promoter 967..995 /created_by="User" /label="AmpR promoter" resistance 1037..1897 /created_by="User" /label="AmpR" promoter 3208..3775 /created_by="User" /label="CMV immediate early promoter" LTR 3793..3976 /created_by="User" /label="HIV-1 5' LTR" other 4638..4871 /created_by="User" /label="RRE" misc_feature 5370..5385 /created_by="User" /modified_by="User" /label="cPPT" /note="Geneious type: Central polypurine tract" misc_difference complement(5508..5750) /created_by="User" /modified_by="User" /Transferred_From="07 TOPO revcas eGFP KpnI AgeI" /Transferred_Similarity="100.00%" /label="polyA" CDS complement(5792..6493) /created_by="User" /label="TagBFP" promoter complement(6576..6635) /created_by="User" /modified_by="User" /Transferred_From="07 TOPO revcas eGFP KpnI AgeI" /Transferred_Similarity="100.00%" /label="minhCMV" promoter 6642..7056 /vntifkey=19 /modified_by="User" /label="SFFV promoter" loxP 7069..7102 /created_by="User" /label="loxP" attB1 7118..7138 /annotation_group="attB" /label="attB1" 5'UTR <7167..7443 /created_by="User" /Transferred_From="ATF4 5: 5’ATF4.GFP extraction" /Transferred_Similarity="100.00%" /modified_by="User" /label="mouse ATF4 5' UTR" exon <7167..7351 /created_by="User" /Transferred_From="ATF4 5: 5’ATF4.GFP extraction" /Transferred_Similarity="100.00%" /modified_by="User" /label="exon 1" misc_feature 7248..7259 /created_by="User" /Transferred_From="ATF4 5: 5’ATF4.GFP extraction" /Transferred_Similarity="100.00%" /modified_by="User" /label="uORF1" misc_feature 7347..7526 /created_by="User" /Transferred_From="ATF4 5: 5’ATF4.GFP extraction" /Transferred_Similarity="100.00%" /modified_by="User" /label="uORF2" exon 7352..>7564 /created_by="User" /Transferred_From="ATF4 5: 5’ATF4.GFP extraction" /Transferred_Similarity="100.00%" /modified_by="User" /label="exon 2" CDS 7444..8301 /created_by="User" /Transferred_From="ATF4 5: 5’ATF4.GFP extraction" /Transferred_Similarity="100.00%" /modified_by="User" /label="ATF4-eGFP fusion ORF" CDS 7444..>7564 /created_by="User" /Transferred_From="ATF4 5: 5’ATF4.GFP extraction" /Transferred_Similarity="100.00%" /modified_by="User" /label="ATF4 start" CDS 7585..8304 /created_by="User" /modified_by="User" /Transferred_From="ATF4 5: 5’ATF4.GFP extraction" /Transferred_Similarity="100.00%" /label="eGFP" attB2 complement(8324..8344) /annotation_group="attB" /label="attB2" loxP 8388..8421 /created_by="User" /modified_by="User" /label="loxP" WPRE 8429..9004 /created_by="User" /label="WPRE" misc_feature 9085..9100 /created_by="User" /modified_by="User" /label="cPPT" /note="Geneious type: Central polypurine tract" LTR 9155..9335 /created_by="User" /label="HIV-1 5' LTR" ORIGIN 1 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 61 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc cagttccgcc 121 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg 181 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcgtc 241 gagacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt 301 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc 361 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt 421 tgcgcagcct gaatggcgaa tggcgcgacg cgccctgtag cggcgcatta agcgcggcgg 481 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt 541 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc 601 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg 661 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga 721 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc 781 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa 841 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa 901 tttcccaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta 961 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata 1021 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc 1081 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga 1141 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct 1201 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg 1261 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta 1321 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat 1381 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt 1441 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga 1501 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga 1561 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga 1621 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc 1681 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc 1741 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg 1801 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat 1861 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata 1921 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct 1981 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga 2041 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 2101 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc 2161 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct 2221 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc 2281 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt 2341 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 2401 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct 2461 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag 2521 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag 2581 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg 2641 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg 2701 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac 2761 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt 2821 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat 2881 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc 2941 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc 3001 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca 3061 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc 3121 ttggccattg catacgttgt atccatatca taatatgtac atttatattg gctcatgtcc 3181 aacattaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg 3241 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 3301 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 3361 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 3421 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga 3481 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg 3541 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat 3601 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 3661 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc 3721 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc 3781 tcgtttagtg aaccggggtc tctctggtta gaccagatct gagcctggga gctctctggc 3841 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg 3901 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg 3961 tggaaaatct ctagcagtgg cgcccgaaca gggacctgaa agcgaaaggg aaaccagagc 4021 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac 4081 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga 4141 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag 4201 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat 4261 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc 4321 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa 4381 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga 4441 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga 4501 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta 4561 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa 4621 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact 4681 atgggcgcag cctcaatgac gctgacggta caggccagac aattattgtc tggtatagtg 4741 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca 4801 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat 4861 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct 4921 tggaatgcta gttggagtaa taaatctctg gaacagattg gaatcacacg acctggatgg 4981 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc 5041 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt 5101 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag 5161 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc 5221 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc 5281 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga 5341 acggatctcg acggtatcgg ttaactttta aaagaaaagg ggggattggg gggtacagtg 5401 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac 5461 aaattacaaa aattcaaaat tttatcgatc acgagactag cctcgagtga tcataatcag 5521 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa 5581 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg 5641 ttacaaataa ggcaatagca tcacaaattt cacaaataag gcattttttt cactgcattc 5701 tagttttggt ttgtccaaac tcatcaatgt atcttatcat gtctggatct caaatccctc 5761 ggaagctgcg cctgtcatcg aattcaccgg tctaattaag cttgtgcccc agtttgctag 5821 ggaggtcgca gtatctggcc actgccacct cgtgctgctc gacgtaggtc tcgttgttgg 5881 cctccttgat tctttccagt ctgtagtcca catagtagac gccaggcatc ttgaggttct 5941 tagcgggttt cttggatcta tatgtggtct tgatgtttgc gatcagatgg ctcccgccca 6001 cgagcttcag ggccatgtcg tttctgcctt ccaggccgcc gtcagcgggg tacagcgtct 6061 cggtgaaggc ctcccagccg agtgttttct tctgcatcac agggccgttg gatgtgaagt 6121 tcacccctct gatcttgacg ttgtagatga ggcagccgtc ctggaggctg gtgtcctggg 6181 tagcggtcag cacgcccccg tcttcgtatg tggtgactct ctcccatgtg aagccctcag 6241 ggaaggactg cttgaagaag tcggggatgc cctgggtgtg gttgatgaag gtcttgctgc 6301 cgtagaggaa gctagtagcc aggatgtcga aggcgaaggg gagagggccg ccctcgacca 6361 ccttgattct catggtctgg gtgccctcgt agggcttgcc ttcgccctcg gatgtgcact 6421 tgaagtgatg gttgtccacg gtgccctcca tgtacagctt catgtgcatg ttctccttaa 6481 tcagctcgct catgatggta ccgaattccg cggaggctgg atcggtcccg gtgtcttcta 6541 tggaggtcaa aacagcgtgg atggcgtctc caggcgatct gacggttcac taaacgagct 6601 ctgcttatat aggcctccca ccgtacacgc ctaccactag taatgaaaga ccccacctgt 6661 aggtttggca agctagctgc agtaacgcca ttttgcaagg catggaaaaa taccaaacca 6721 agaatagaga agttcagatc aagggcgggt acatgaaaat agctaacgtt gggccaaaca 6781 ggatatctgc ggtgagcagt ttcggccccg gcccggggcc aagaacagat ggtcaccgca 6841 gtttcggccc cggcccgagg ccaagaacag atggtcccca gatatggccc aaccctcagc 6901 agtttcttaa gacccatcag atgtttccag gctcccccaa ggacctgaaa tgaccctgcg 6961 ccttatttga attaaccaat cagcctgctt ctcgcttctg ttcgcgcgct tctgcttccc 7021 gagctctata aaagagctca caacccctca ctcggcttaa ttaagatcat aacttcgtat 7081 agcatacatt atacgaagtt atcccagctt gatatcacaa gtttgtacaa aaaagcaggc 7141 tccgcggccg cccccttcca ccagcttttc tgcttgctgt ctgccggttt gagttgtgcg 7201 ctcgggtgtc cctttcctct tcccctcccg cagggcttgc ggccaccatg gcgtattaga 7261 ggcagcagtg cctgcggcag cgttggcctt tgcagcggcg gcagcagcac caggctctgc 7321 agcggcaacc cccaccggcc taagccatgg cgctcttcac gaaatccagc agcagtgttg 7381 ctgtaacgga caaagatacc ttcgagttaa gcacattcct ggaatccagc aaagccccac 7441 aacatgaccg agatgagctt cctgaacagc gaagtgttgg cgggggactt gatgtccccc 7501 ttcgaccagt cgggtttggg ggctgaagaa agcctaggtc tcttagatga ctatctggag 7561 gtgggggatc caccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg 7621 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc 7681 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc 7741 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc 7801 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc 7861 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag 7921 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag 7981 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat 8041 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc 8101 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc 8161 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc 8221 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc 8281 ggcatggacg agctgtacaa gtaaaagggt gggcgcgccg acccagcttt cttgtacaaa 8341 gtggtgatat cgaattcctg cagcccgggg gatccactag ttctagaata acttcgtata 8401 gcatacatta tacgaagtta ttcgacaatc aacctctgga ttacaaaatt tgtgaaagat 8461 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc 8521 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct 8581 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca 8641 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt 8701 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg 8761 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga 8821 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt 8881 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc 8941 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt 9001 gggccgcctc cccgcctgga attcgagctc ggtaccttta agaccaatga cttacaaggc 9061 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc 9121 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg 9181 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc 9241 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct 9301 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat 9361 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc 9421 agcttataat ggttacaaat aaagcaatag catcac //