LOCUS       HisMS2_PLP_NucPhos_pac  5871 bp ds-DNA     circular     28-MAR-2020
DEFINITION  .
KEYWORDS    "Antibiotic Resistance"                                            
FEATURES             Location/Qualifiers
     misc_feature    1643..1645
                     /label="Start of second coat dimer"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    2126..2170
                     /label="Spacer"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     promoter        5788..5817
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             45..1226
                     /label="Maturation Protein"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     promoter        5736..5754
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     terminator      3469..3530
                     /label="T7 terminator"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             2190..3449
                     /label="N CDS (nucleocapsid phosphoprotein)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    80..80
                     /label="g->a (remove BsaI)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     gene            2190..3449
                     /label="N gene"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     RBS             5837..5859
                     /label="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1250..1639
                     /label="Coat Protein"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1993..1993
                     /label="c->t (remove BsaI)"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2203..2222
                     /label="2019-nCoV_N1 Forward Primer"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     terminator      2064..2125
                     /label="T7 terminator"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(3129..3146)
                     /label="2019-nCoV_N2 Reverse Primer"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3080..3099
                     /label="2019-nCoV_N2 Forward Primer"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3104..3126
                     /label="2019-nCoV_N2 Probe"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    1682..1699
                     /label="His Tag"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     protein_bind    5755..5779
                     /label="lac operator"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        2171..2189
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1250..2050
                     /label="coat protein dimer"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             complement(4497..5294)
                     /label="KanR"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     rep_origin      complement(3720..4402)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(2251..2274)
                     /label="2019-nCoV_N1 Reverse Primer"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    2597..2618
                     /label="2019-nCoV_N3 Forward Primer"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(2874..2906)
                     /label="RPA R"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    875..875
                     /label="t->c (remove BsmBI)"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    2786..2818
                     /label="RPA F"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    629..629
                     /label="g->a (remove BspMI)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1585..1585
                     /label="c->t (remove BsaI)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2225..2248
                     /label="2019-nCoV_N1 Probe"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    complement(2648..2668)
                     /label="2019-nCoV_N3 Reverse Primer"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
ORIGIN
        1 ATCGCTGTAG GTAGCCGGAA TTCCATTCCT AGGAGGTTTG ACCTgtgcga gcttttagta
       61 cccttgatag ggagaacgaa accttcgtcc cctccgttcg cgtttacgcg gacggtgaga
      121 ctgaagataa ctcattctct ttaaaatatc gttcgaactg gactcccggt cgttttaact
      181 cgactggggc caaaacgaaa cagtggcact acccctctcc gtattcacgg ggggcgttaa
      241 gtgtcacatc gatagatcaa ggtgcctaca agcgaagtgg gtcatcgtgg ggtcgcccgt
      301 acgaggagaa agccggtttc ggcttctccc tcgacgcacg ctcctgctac agcctcttcc
      361 ctgtaagcca aaacttgact tacatcgaag tgccgcagaa cgttgcgaac cgggcgtcga
      421 ccgaagtcct gcaaaaggtc acccagggta attttaacct tggtgttgct ttagcagagg
      481 ccaggtcgac agcctcacaa ctcgcgacgc aaaccattgc gctcgtgaag gcgtacactg
      541 ccgctcgtcg cggtaattgg cgccaggcgc tccgctacct tgccctaaac gaagatcgaa
      601 agtttcgatc aaaacacgtg gccggcagat ggttggagtt gcagttcggt tggttaccac
      661 taatgagtga tatccagggt gcatatgaga tgcttacgaa ggttcacctt caagagtttc
      721 ttcctatgag agccgtacgt caggtcggta ctaacatcaa gttagatggc cgtctgtcgt
      781 atccagctgc aaacttccag acaacgtgca acatatcgcg acgtatcgtg atatggtttt
      841 acataaacga tgcacgtttg gcatggttgt cgtccctagg tatcttgaac ccactaggta
      901 tagtgtggga aaaggtgcct ttctcattcg ttgtcgactg gctcctacct gtaggtaaca
      961 tgctcgaggg ccttacggcc cccgtgggat gctcctacat gtcaggaaca gttactgacg
     1021 taataacggg tgagtccatc ataagcgttg acgctcccta cgggtggact gtggagagac
     1081 agggcactgc taaggcccaa atctcagcca tgcatcgagg ggtacaatcc gtatggccaa
     1141 caactggcgc gtacgtaaag tctcctttct cgatggtcca taccttagat gcgttagcat
     1201 taatcaggca acggctctct agatagAGCC CTCAACCGGA GTTTGAAGCA TGGCTTCTAA
     1261 CTTTACTCAG TTCGTTCTCG TCGACAATGG CGGAACTGGC GACGTGACTG TCGCCCCAAG
     1321 CAACTTCGCT AACGGGGTCG CTGAATGGAT CAGCTCTAAC TCGCGTTCAC AGGCTTACAA
     1381 AGTAACCTGT AGCGTTCGTC AGAGCTCTGC GCAGAATCGC AAATACACCA TCAAAGTCGA
     1441 GGTGCCTAAA GTGGCAACCC AGACTGTTGG TGGTGTAGAG CTTCCTGTAG CCGCATGGCG
     1501 TTCGTACTTA AATATGGAAC TAACCATTCC AATTTTCGCT ACGAATTCCG ACTGCGAGCT
     1561 TATTGTTAAG GCAATGCAAG GTCTTCTAAA AGATGGAAAC CCGATTCCCT CAGCAATCGC
     1621 AGCAAACTCC GGCATCTACG CTAACTTTAC TCAGTTCGTT CTCGTCGACA ATGGCGGTAC
     1681 CCATCACCAT CACCATCACG GTACCGGCGA CGTGACTGTC GCCCCAAGCA ACTTCGCTAA
     1741 CGGGGTCGCT GAATGGATCA GCTCTAACTC GCGTTCACAG GCTTACAAAG TAACCTGTAG
     1801 CGTtCGTCAG AGCTCTGCGC AGAATCGCAA ATACACCATC AAAGTCGAGG TGCCTAAAGT
     1861 GGCAACCCAG ACTGTTGGTG GTGTAGAGCT TCCTGTAGCC GCATGGCGTT CGTACTTAAA
     1921 TATGGAACTA ACCATTCCAA TTTTCGCTAC GAATTCCGAC TGCGAGCTTA TTGTTAAGGC
     1981 AATGCAAGGT CTTCTAAAAG ATGGAAACCC GATTCCCTCA GCAATCGCAG CAAACTCCGG
     2041 CATCTACTAA TAGACGCCGG CCAccgctga gcaataacta gcataacccc ttggggcctc
     2101 taaacgggtc ttgaggggtt ttttgTCGAA CAGAAAGTAA TCGTATTGTA CACGGCCGCA
     2161 TAATCGAAAT TAATACGACT CACTATAGGa tgtctgataa tggaccccaa aatcagcgaa
     2221 atgcaccccg cattacgttt ggtggaccct cagattcaac tggcagtaac cagaatggag
     2281 aacgcagtgg ggcgcgatca aaacaacgtc ggccccaagg tttacccaat aatactgcgt
     2341 cttggttcac cgctctcact caacatggca aggaagacct taaattccct cgaggacaag
     2401 gcgttccaat taacaccaat agcagtccag atgaccaaat tggctactac cgaagagcta
     2461 ccagacgaat tcgtggtggt gacggtaaaa tgaaagatct cagtccaaga tggtatttct
     2521 actacctagg aactgggcca gaagctggac ttccctatgg tgctaacaaa gacggcatca
     2581 tatgggttgc aactgaggga gccttgaata caccaaaaga tcacattggc acccgcaatc
     2641 ctgctaacaa tgctgcaatc gtgctacaac ttcctcaagg aacaacattg ccaaaaggct
     2701 tctacgcaga agggagcaga ggcggcagtc aagcctcttc tcgttcctca tcacgtagtc
     2761 gcaacagttc aagaaattca actccaggca gcagtagggg aacttctcct gctagaatgg
     2821 ctggcaatgg cggtgatgct gctcttgctt tgctgctgct tgacagattg aaccagcttg
     2881 agagcaaaat gtctggtaaa ggccaacaac aacaaggcca aactgtcact aagaaatctg
     2941 ctgctgaggc ttctaagaag cctcggcaaa aacgtactgc cactaaagca tacaatgtaa
     3001 cacaagcttt cggcagacgt ggtccagaac aaacccaagg aaattttggg gaccaggaac
     3061 taatcagaca aggaactgat tacaaacatt ggccgcaaat tgcacaattt gcccccagcg
     3121 cttcagcgtt cttcggaatg tcgcgcattg gcatggaagt cacaccttcg ggaacgtggt
     3181 tgacctacac aggtgccatc aaattggatg acaaagatcc aaatttcaaa gatcaagtca
     3241 ttttgctgaa taagcatatt gacgcataca aaacattccc accaacagag cctaaaaagg
     3301 acaaaaagaa gaaggctgat gaaactcaag ccttaccgca gagacagaag aaacagcaaa
     3361 ctgtgactct tcttcctgct gcagatttgg atgatttctc caaacaattg caacaatcca
     3421 tgagcagtgc tgactcaact caggcctaaA CATGAGGATC ACCCATGTcc gctgagcaat
     3481 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg TAATTTCACT
     3541 GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAACATGGTC ATAGCTGTTT
     3601 CCTTGCGTAT TGGGCGCTCT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG
     3661 GGTAAAGCCT GGGGTGCCTA ATGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG
     3721 GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA
     3781 CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT
     3841 GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC
     3901 TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG
     3961 GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC
     4021 TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA
     4081 CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG
     4141 TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT
     4201 CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC
     4261 ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA
     4321 TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA
     4381 CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT
     4441 TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAT
     4501 TAGAAAAATT CATCCAGCAG ACGATAAAAC GCAATACGCT GGCTATCCGG TGCCGCAATG
     4561 CCATACAGCA CCAGAAAACG ATCCGCCCAT TCGCCGCCCA GTTCTTCCGC AATATCACGG
     4621 GTGGCCAGCG CAATATCCTG ATAACGATCC GCCACGCCCA GACGGCCGCA ATCAATAAAG
     4681 CCGCTAAAAC GGCCATTTTC CACCATAATG TTCGGCAGGC ACGCATCACC ATGGGTCACC
     4741 ACCAGATCTT CGCCATCCGG CATGCTCGCT TTCAGACGCG CAAACAGCTC TGCCGGTGCC
     4801 AGGCCCTGAT GTTCTTCATC CAGATCATCC TGATCCACCA GGCCCGCTTC CATACGGGTA
     4861 CGCGCACGTT CAATACGATG TTTCGCCTGA TGATCAAACG GACAGGTCGC CGGGTCCAGG
     4921 GTATGCAGAC GACGCATGGC ATCCGCCATA ATGCTCACTT TTTCTGCCGG CGCCAGATGG
     4981 CTAGACAGCA GATCCTGACC CGGCACTTCG CCCAGCAGCA GCCAATCACG GCCCGCTTCG
     5041 GTCACCACAT CCAGCACCGC CGCACACGGA ACACCGGTGG TGGCCAGCCA GCTCAGACGC
     5101 GCCGCTTCAT CCTGCAGCTC GTTCAGCGCA CCGCTCAGAT CGGTTTTCAC AAACAGCACC
     5161 GGACGACCCT GCGCGCTCAG ACGAAACACC GCCGCATCAG AGCAGCCAAT GGTCTGCTGC
     5221 GCCCAATCAT AGCCAAACAG ACGTTCCACC CACGCTGCCG GGCTACCCGC ATGCAGGCCA
     5281 TCCTGTTCAA TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT
     5341 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC
     5401 ACATTTCCCC GAAAAGTGCC ACCTAAATTG TAAGCGTTAA TATTTTGTTA AAATTCGCGT
     5461 TAAATTTTTG TTAAATCAGC TCATTTTTTA ACCAATAGGC CGAAATCGGC AAAATCCCTT
     5521 ATAAATCAAA AGAATAGACC GAGATAGGGT TGAGTGGCCG CTACAGGGCG CTCCCATTCG
     5581 CCATTCAGGC TGCGCAACTG TTGGGAAGGG CGTTTCGGTG CGGGCCTCTT CGCTATTACG
     5641 CCAGCTGGCG AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTTC
     5701 CCAGTCACGA CGTTGTAAAA CGACGGCCAG TGAGGtaata cgactcacta taggggaatt
     5761 gtgagcggat aacaattccc ctctagattt acactttatg cttccggctc gtatgtttct
     5821 agagaattca aataattttg tttaacttta agaaggagat ttaaatTGGC T
//