LOCUS       HisMS2_PLP_Env_pac      4839 bp ds-DNA     circular     04-MAR-2020
DEFINITION  .
KEYWORDS    "Antibiotic Resistance"                                            
FEATURES             Location/Qualifiers
     misc_feature    1617..1617
                     /label="t->c (remove BsmBI)"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    complement(3047..3068)
                     /label="E_Sarbeco_R2"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2424..2441
                     /label="His Tag"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     promoter        2913..2931
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     terminator      2806..2867
                     /label="T7 terminator"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1992..2792
                     /label="coat protein dimer"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2735..2735
                     /label="c->t (remove BsaI)"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     protein_bind    626..650
                     /label="lac operator"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    822..822
                     /label="g->a (remove BsaI)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        659..688
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(join(4207..4839,1..165))
                     /label="KanR"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    3019..3044
                     /label="E_Sarbeco_P1"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1992..2381
                     /label="Coat Protein"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     rep_origin      complement(3430..4112)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    2956..2981
                     /label="E_Sarbeco_F1"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        607..625
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     gene            2932..3159
                     /label="E gene"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    2385..2387
                     /label="Start of second coat dimer"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             2932..3159
                     /label="E CDS (envelope protein)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2327..2327
                     /label="c->t (remove BsaI)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     terminator      3179..3240
                     /label="T7 terminator"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     RBS             708..730
                     /label="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1371..1371
                     /label="g->a (remove BspMI)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2868..2912
                     /label="Spacer"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             787..1968
                     /label="Maturation Protein"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
ORIGIN
        1 ACCGCTCAGA TCGGTTTTCA CAAACAGCAC CGGACGACCC TGCGCGCTCA GACGAAACAC
       61 CGCCGCATCA GAGCAGCCAA TGGTCTGCTG CGCCCAATCA TAGCCAAACA GACGTTCCAC
      121 CCACGCTGCC GGGCTACCCG CATGCAGGCC ATCCTGTTCA ATCATACTCT TCCTTTTTCA
      181 ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT
      241 TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTAAATT
      301 GTAAGCGTTA ATATTTTGTT AAAATTCGCG TTAAATTTTT GTTAAATCAG CTCATTTTTT
      361 AACCAATAGG CCGAAATCGG CAAAATCCCT TATAAATCAA AAGAATAGAC CGAGATAGGG
      421 TTGAGTGGCC GCTACAGGGC GCTCCCATTC GCCATTCAGG CTGCGCAACT GTTGGGAAGG
      481 GCGTTTCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA
      541 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA
      601 GTGAGGtaat acgactcact ataggggaat tgtgagcgga taacaattcc cctctagatt
      661 tacactttat gcttccggct cgtatgtttc tagagaattc aaataatttt gtttaacttt
      721 aagaaggaga tttaaatTGG CTATCGCTGT AGGTAGCCGG AATTCCATTC CTAGGAGGTT
      781 TGACCTgtgc gagcttttag tacccttgat agggagaacg aaaccttcgt cccctccgtt
      841 cgcgtttacg cggacggtga gactgaagat aactcattct ctttaaaata tcgttcgaac
      901 tggactcccg gtcgttttaa ctcgactggg gccaaaacga aacagtggca ctacccctct
      961 ccgtattcac ggggggcgtt aagtgtcaca tcgatagatc aaggtgccta caagcgaagt
     1021 gggtcatcgt ggggtcgccc gtacgaggag aaagccggtt tcggcttctc cctcgacgca
     1081 cgctcctgct acagcctctt ccctgtaagc caaaacttga cttacatcga agtgccgcag
     1141 aacgttgcga accgggcgtc gaccgaagtc ctgcaaaagg tcacccaggg taattttaac
     1201 cttggtgttg ctttagcaga ggccaggtcg acagcctcac aactcgcgac gcaaaccatt
     1261 gcgctcgtga aggcgtacac tgccgctcgt cgcggtaatt ggcgccaggc gctccgctac
     1321 cttgccctaa acgaagatcg aaagtttcga tcaaaacacg tggccggcag atggttggag
     1381 ttgcagttcg gttggttacc actaatgagt gatatccagg gtgcatatga gatgcttacg
     1441 aaggttcacc ttcaagagtt tcttcctatg agagccgtac gtcaggtcgg tactaacatc
     1501 aagttagatg gccgtctgtc gtatccagct gcaaacttcc agacaacgtg caacatatcg
     1561 cgacgtatcg tgatatggtt ttacataaac gatgcacgtt tggcatggtt gtcgtcccta
     1621 ggtatcttga acccactagg tatagtgtgg gaaaaggtgc ctttctcatt cgttgtcgac
     1681 tggctcctac ctgtaggtaa catgctcgag ggccttacgg cccccgtggg atgctcctac
     1741 atgtcaggaa cagttactga cgtaataacg ggtgagtcca tcataagcgt tgacgctccc
     1801 tacgggtgga ctgtggagag acagggcact gctaaggccc aaatctcagc catgcatcga
     1861 ggggtacaat ccgtatggcc aacaactggc gcgtacgtaa agtctccttt ctcgatggtc
     1921 cataccttag atgcgttagc attaatcagg caacggctct ctagatagAG CCCTCAACCG
     1981 GAGTTTGAAG CATGGCTTCT AACTTTACTC AGTTCGTTCT CGTCGACAAT GGCGGAACTG
     2041 GCGACGTGAC TGTCGCCCCA AGCAACTTCG CTAACGGGGT CGCTGAATGG ATCAGCTCTA
     2101 ACTCGCGTTC ACAGGCTTAC AAAGTAACCT GTAGCGTTCG TCAGAGCTCT GCGCAGAATC
     2161 GCAAATACAC CATCAAAGTC GAGGTGCCTA AAGTGGCAAC CCAGACTGTT GGTGGTGTAG
     2221 AGCTTCCTGT AGCCGCATGG CGTTCGTACT TAAATATGGA ACTAACCATT CCAATTTTCG
     2281 CTACGAATTC CGACTGCGAG CTTATTGTTA AGGCAATGCA AGGTCTTCTA AAAGATGGAA
     2341 ACCCGATTCC CTCAGCAATC GCAGCAAACT CCGGCATCTA CGCTAACTTT ACTCAGTTCG
     2401 TTCTCGTCGA CAATGGCGGT ACCCATCACC ATCACCATCA CGGTACCGGC GACGTGACTG
     2461 TCGCCCCAAG CAACTTCGCT AACGGGGTCG CTGAATGGAT CAGCTCTAAC TCGCGTTCAC
     2521 AGGCTTACAA AGTAACCTGT AGCGTtCGTC AGAGCTCTGC GCAGAATCGC AAATACACCA
     2581 TCAAAGTCGA GGTGCCTAAA GTGGCAACCC AGACTGTTGG TGGTGTAGAG CTTCCTGTAG
     2641 CCGCATGGCG TTCGTACTTA AATATGGAAC TAACCATTCC AATTTTCGCT ACGAATTCCG
     2701 ACTGCGAGCT TATTGTTAAG GCAATGCAAG GTCTTCTAAA AGATGGAAAC CCGATTCCCT
     2761 CAGCAATCGC AGCAAACTCC GGCATCTACT AATAGACGCC GGCCAccgct gagcaataac
     2821 tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgTCG AACAGAAAGT
     2881 AATCGTATTG TACACGGCCG CATAATCGAA ATTAATACGA CTCACTATAG Gatgtactca
     2941 ttcgtttcgg aagagacagg tacgttaata gttaatagcg tacttctttt tcttgctttc
     3001 gtggtattct tgctagttac actagccatc cttactgcgc ttcgattgtg tgcgtactgc
     3061 tgcaatattg ttaacgtgag tcttgtaaaa ccttcttttt acgtttactc tcgtgttaaa
     3121 aatctgaatt cttctagagt tcctgatctt ctggtctaaA CATGAGGATC ACCCATGTcc
     3181 gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg
     3241 TAATTTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAACATGGTC
     3301 ATAGCTGTTT CCTTGCGTAT TGGGCGCTCT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT
     3361 CGGTCGTTCG GGTAAAGCCT GGGGTGCCTA ATGAGCAAAA GGCCAGCAAA AGGCCAGGAA
     3421 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA
     3481 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC
     3541 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA
     3601 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA
     3661 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA
     3721 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA
     3781 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG
     3841 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG
     3901 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG
     3961 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG
     4021 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA
     4081 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT
     4141 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC
     4201 TGACAGTTAT TAGAAAAATT CATCCAGCAG ACGATAAAAC GCAATACGCT GGCTATCCGG
     4261 TGCCGCAATG CCATACAGCA CCAGAAAACG ATCCGCCCAT TCGCCGCCCA GTTCTTCCGC
     4321 AATATCACGG GTGGCCAGCG CAATATCCTG ATAACGATCC GCCACGCCCA GACGGCCGCA
     4381 ATCAATAAAG CCGCTAAAAC GGCCATTTTC CACCATAATG TTCGGCAGGC ACGCATCACC
     4441 ATGGGTCACC ACCAGATCTT CGCCATCCGG CATGCTCGCT TTCAGACGCG CAAACAGCTC
     4501 TGCCGGTGCC AGGCCCTGAT GTTCTTCATC CAGATCATCC TGATCCACCA GGCCCGCTTC
     4561 CATACGGGTA CGCGCACGTT CAATACGATG TTTCGCCTGA TGATCAAACG GACAGGTCGC
     4621 CGGGTCCAGG GTATGCAGAC GACGCATGGC ATCCGCCATA ATGCTCACTT TTTCTGCCGG
     4681 CGCCAGATGG CTAGACAGCA GATCCTGACC CGGCACTTCG CCCAGCAGCA GCCAATCACG
     4741 GCCCGCTTCG GTCACCACAT CCAGCACCGC CGCACACGGA ACACCGGTGG TGGCCAGCCA
     4801 GCTCAGACGC GCCGCTTCAT CCTGCAGCTC GTTCAGCGC
//