LOCUS       Exported                2880 bp ds-DNA     circular SYN 22-JUN-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jun 29, 2020 from SnapGene Viewer 5.1.4.1
            https://www.snapgene.com
COMMENT     Serial Cloner Genbank Format
            SerialCloner_Type=DNA
            SerialCloner_Comments=
            SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      complement(268..295)
                     /locus_tag="rrnB T2"
                     /label=rrnB T2 terminator
     terminator      complement(427..470)
                     /locus_tag="rrnB T1"
                     /label=rrnB T1 terminator
     primer_bind     536..553
                     /locus_tag="M13-fwd"
                     /label=M13-fwd
     misc_recomb     593..688
                     /locus_tag="attL4"
                     /label=attL4
     misc_recomb     complement(668..688)
                     /locus_tag="attB4"
                     /label=attB4
     misc_feature    698..703
                     /locus_tag="KpnI"
                     /label=KpnI
     misc_feature    730..745
                     /locus_tag="qa-1F"
                     /label=qa-1F
     misc_feature    750..765
                     /locus_tag="qa-2"
                     /label=qa-2
     misc_feature    770..785
                     /locus_tag="qa-1F(1)"
                     /label=qa-1F
     misc_feature    790..805
                     /locus_tag="qa-2(1)"
                     /label=qa-2
     misc_feature    810..825
                     /locus_tag="qa-1F(2)"
                     /label=qa-1F
     misc_feature    828..898
                     /locus_tag="E1b"
                     /label=E1b
     misc_feature    932..937
                     /locus_tag="BsrGI"
                     /label=BsrGI
     misc_recomb     938..1049
                     /locus_tag="AttR1"
                     /label=AttR1
     primer_bind     complement(1121..1140)
                     /locus_tag="T7"
                     /label=T7
     primer_bind     complement(1141..1161)
                     /locus_tag="M13-rev"
                     /label=M13-rev
     CDS             1274..2080
                     /locus_tag="KanR"
                     /label=KanR
     rep_origin      2208..2836
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccga gttaacgcta ccatggagct ccaaataatg
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
      661 ataatgccaa ctttgtatag aaaagttgca aaagctgggt accgagctcc accgcggtgg
      721 cggccgcaag gataaacaat tatcctcacg ggtaatcgct tatcctcaag gataaacaat
      781 tatcctcacg ggtaatcgct tatcctcaag gataaacaat tatcctcgca agggtcgact
      841 ctagagggta tataatggat cccatcgcgt ctcagcctca ctttgagctc ctccacacga
      901 attccctcga cctcgaagac gcgtccaagt ttgtacaaaa aagttgaacg agaaacgtaa
      961 aatgatataa atatcaatat attaaattag attttgcata aaaaacagac tacataatac
     1021 tgtaaaacac aacatatgca gtcactatga accaactact tagatggtat tagtgacctg
     1081 tagaattcga gctctagagc tgcagggcgg ccgcgatatc ccctatagtg agtcgtatta
     1141 catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat
     1201 tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa
     1261 tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa
     1321 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc
     1381 gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa
     1441 aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt
     1501 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac
     1561 cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga
     1621 aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa
     1681 ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa
     1741 cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt
     1801 ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga
     1861 tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg
     1921 acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga
     1981 gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat
     2041 gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg
     2101 gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa
     2161 atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     2221 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     2281 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     2341 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     2401 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     2461 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     2521 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     2581 gcgaacgacc tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct
     2641 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     2701 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     2761 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     2821 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
//
