LOCUS       Exported                3889 bp ds-DNA     circular SYN 29-JUN-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jun 29, 2020 from SnapGene Viewer 5.1.4.1
            https://www.snapgene.com
COMMENT     Ligation of : Vector 54 [2812  nt] : (XmaI[3375] / SacII[684]) to 
            QF2w [1071  nt] : (SacII[3] / XmaI[1077])
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      complement(268..295)
                     /label=rrnB T2
     terminator      complement(427..470)
                     /label=rrnB T1
     primer_bind     536..553
                     /label=M13-fwd
     misc_recomb     570..665
                     /label=AttL1
     misc_feature    690..698
                     /label=Kozak
     misc_feature    699..1757
                     /label=QF2w
     misc_feature    1764..1962
                     /label=SV40 late PolyA trunc
     misc_feature    1810..1943
                     /label=SV40 late core
     misc_recomb     complement(2017..2112)
                     /label=AttL2
     primer_bind     complement(2130..2149)
                     /label=T7
     primer_bind     complement(2150..2170)
                     /label=M13-rev
     CDS             2283..3089
                     /label=KanR
     rep_origin      3217..3845
                     /direction=RIGHT
                     /label=ColE1 origin
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcg aattggagct ccaccgcggg ccgccaccat gccacccaag cgcaaaacgc
      721 ttaacgctgc ggctgaggct aacgctcatg ccgacggaca cgccgacgga aacgccgacg
      781 gacacgtggc caatacggcc gcgtcctcga ataatgcgag gttcgctgat ctcactaaca
      841 tcgatactcc gggtctggga cccacaacta cgaccctgct cgtggaacca gcacgctcaa
      901 agcgtcaacg agtgtcccgc gcatgcgacc agtgccgtgc agcccgagag aaatgcgacg
      961 gaatacagcc tgcgtgtttc ccgtgcgttt cccagggaag gtcctgcact tatcaggctt
     1021 cgccgaaaaa gaggggagtt caaaccggtt atattcgtac gctggagctc gccctcgcct
     1081 ggatgtttga aaatgtcgcg cgttccgaag atgccttgca taacctcctc gtccgtgacg
     1141 ccggacaagg atcagctctg ctcgttggta aagattcgcc ggctgccgag cgactccatg
     1201 cccgttgggc tactagccgt gtcaataaga gcattacccg cctcctccgt cagttggagc
     1261 tccctcctac cgccacggct acggcctcga taatgccgca cgtgatggag cagcctctca
     1321 gtaccagcat taaccccgtc aacgaccgct tcaacggtat tcccaacccc actccgtata
     1381 actccgatgc agctctcgat gctatcactc agaccaacga ttatggaagc gtaaatacac
     1441 atggtatcct ctctacttac ccgccaccgg ctacgcacct taatgaagct tccgtcgctc
     1501 tcgctcccgg tggcgccccc ccccgaccgc ctcctccgta tgttgacagc acgaccaatc
     1561 acccgccgta ccactcgaat ctggttccaa tggcgaactt tggttactcg accgttgatt
     1621 acgatgccat ggttgacgat ttggctagca ttgaatacac ggacgctgtg gatgtcgacc
     1681 cacagtttat gaccaatctg ggattcgttc ctggatgtaa cttctccgac attaatacat
     1741 acaaaaagaa gaaatgaccc gggagcggcc cctctcgagc ctctagaact atagtgagtc
     1801 gtattacgta gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag
     1861 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     1921 cattataagc tgcaataaac aagagatctg atctagagga tcaagcttat cgataccgtc
     1981 gacctcgagg gggggcccgg tacccagctt ttgacccagc tttcttgtac aaagttggca
     2041 ttataagaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata
     2101 aaatcattat ttgccatcca gctgatatcc cctatagtga gtcgtattac atggtcatag
     2161 ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata
     2221 aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg
     2281 ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt aaattccaac atggatgctg
     2341 atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc
     2401 gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg
     2461 ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc
     2521 cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc
     2581 ccggaaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg
     2641 atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta
     2701 acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg
     2761 atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa
     2821 tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg
     2881 ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa
     2941 tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt
     3001 cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc
     3061 agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacact
     3121 ggcagagcat tacgctgact tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg
     3181 tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     3241 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     3301 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     3361 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     3421 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     3481 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     3541 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     3601 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     3661 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     3721 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     3781 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     3841 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgtt
//
