LOCUS       Exported               11237 bp ds-DNA     circular SYN 29-JUN-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11237)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jun 29, 2020 from SnapGene Viewer 5.1.4.1
            https://www.snapgene.com
COMMENT     [DEFINITION]: synthetic circular DNA
[LOCUS]: Exported
[ACCESSION]: 
            .
[SOURCE]: 1..11237
[CDS]: 7630..8301
FEATURES             Location/Qualifiers
     source          1..11237
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1..4741
                     /label=source [*]
                     /note="organism=""recombinant plasmid"" - mol_type=""other 
                     DNA"" - 1..11237"
     source          4745..11237
                     /label=source [*]
                     /note="organism=""recombinant plasmid"" - mol_type=""other 
                     DNA"" - 1..11237"
     primer_bind     1169..1189
                     /label=M13-rev
     misc_recomb     complement(1205..1225)
                     /label=attB4
     misc_feature    1253..4735
                     /label=Ubi Promoter
                     /note="note=""Ubi Promoter"" - 1253..4735"
     misc_feature    4761..4784
                     /label=AttB1
     misc_feature    4798..4806
                     /label=Kozak
                     /note="note=""Kozak"" - 4798..4806"
     misc_feature    4807..4830
                     /label=NLS
                     /note="note=""NLS"" - 4807..4830"
     misc_feature    4831..5385
                     /label=QF_DBD
                     /note="note=""QF"" - 4831..5385"
     misc_feature    5407..5808
                     /label=Gal4_AD
     misc_feature    5815..6013
                     /label=SV40
                     /note="note=""SV40 late PolyA trunc"" - 5815..6013"
     misc_feature    6065..6088
                     /label=AttB2
     misc_feature    6097..6102
                     /label=AgeI
                     /note="note=""AgeI"" - 6097..6102"
     misc_feature    6133..6138
                     /label=SpeI
                     /note="note=""SpeI"" - 6133..6138"
     misc_feature    6205..6210
                     /label=KpnI
                     /note="note=""KpnI"" - 6205..6210"
     misc_feature    6212..6232
                     /label=AttB3
     polyA_signal    6276..6467
                     /label=SV40 late polyA
     misc_feature    6528..7431
                     /label=cmlc2 Promoter
                     /note="note=""cmlc2 Promoter"" - 6528..7431"
     misc_feature    7510..7626
                     /label=Membrane Targeting Sequence
                     /note="note=""Membrane Targeting Sequence"" - 7510..7626"
     CDS             7630..8301
                     /label=mRFP1
     misc_feature    8311..8443
                     /label=SV40pA Short_misc_feature
                     /note="note=""SV40pA Short"" - 8311..8443"
     primer_bind     complement(9051..9070)
                     /label=T3
     primer_bind     complement(9088..9108)
                     /label=M13-rev
     misc_binding    complement(9114..9136)
                     /label=LacO
     promoter        complement(9141..9170)
                     /label=lac
     rep_origin      complement(9476..10104)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(10256..10915)
                     /label=AmpR
     promoter        complement(11155..11183)
                     /label=Amp prom_promoter
                     /note="note=""Amp prom"" - complement(11155..11183)"
     promoter        complement(11155..11183)
                     /label=Amp prom
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccgggctg gctaagaact catcagcctc
      361 cccggtccat ctacccacgt accaatgcac caattggcca caatgacggc tactacatgg
      421 tgccattcct tcctctttat aggaatggag actacctcct gtccaacaag gctcttggat
      481 acgagtacgc ctacctgttg gacccaggtc attgcacaac accagaaatg ccctctgatc
      541 tgcaaaagac gtgaatatct gttcagacac ccatatccac tctgttccac acaggtcaga
      601 ggtttgtcca ggagttcttg acagaggtgt aaaaagtact caaaaatttt actcaagtga
      661 aagtacaagt acttagggaa aattttactc aattaaaagt aaaagtatct ggctagaatc
      721 ttacttgagt aaaagtaaaa aagtactcca ttaaaattgt acttgagtat taaggaagta
      781 aaagtaaaag caagaaagaa aactagagat tcttgtttaa gcttttaatc tcaaaaaaca
      841 ttaaatgaaa tgcatacaag gttttatcct gctttagaac tgtttgtatt taattatcaa
      901 actataagac agacaatcta atgccagtac acgctactca aagttgtaaa acctcagatt
      961 taacttcagt agaagctgat tctcaaaatt gttagtgtca agcctagctc ttttggggct
     1021 gaaaagcaat cctgcagtgc tgaaaagcct ctcacaggca gccgatgcgg gaagaggtgt
     1081 attagtcttg atagagaggc tgcaaatagc aggaaacgtg agcagagact ccctggtgtc
     1141 tgaaacacag gccagatggg ccctcgagca ggaaacagct atgaccatga ttacgccaag
     1201 ctatcaactt tgtatagaaa agttggctcc gaattcgccc ttaaaactcg agaccagcaa
     1261 agttctagaa tttgtcgaaa catttatgtt atatatttcc tgaaaaaaat tctgagtaag
     1321 ttcttaagtg ttattgccag caacataaac aacagacggc aaaatgaata aatgataaca
     1381 aagcagtagg cttaaataaa cctaattttt ataggctgtt ctctacaacc ctcaaacagt
     1441 gattagtttt gtacttataa acttgccctt tcattcatat ttcaagaaaa ttggttcaga
     1501 agatctggat attctagcag ttgttcaagc tcatggaggg atcagtgacc tgattccaca
     1561 atgactaggc ctaatccaga aattagatga ctgtcaacat aaaaaggcac agcactcact
     1621 agctgcccta tatattttat tatattttac atatattatt ttatttattt agctctgagt
     1681 gctgtacttt ctggttaaag aaaactgctt acaacagcta acctgtacta cctcaggctc
     1741 agggaatttg gaacaggttt gtctggtttg tttctttaac catgcatgct tgttttcaac
     1801 tatggcaaca cagtcacatg ggacattaca gaaatgattt gtcgatgaca tgcgactttt
     1861 ctttaataaa gcgcaaagat cccaaaaagc aaacttttaa caaaaatcat ataattatat
     1921 tttcaatcca gctttgtagc aactttgtgc tgctgttcac tcagcaacag atagtcagta
     1981 taaggtcagt gtgtctcaaa gcagtgccat ctgtttcaca cattgcgttc tatatataag
     2041 tgtgctggtt gacacgacac tgtataaggc ctaggctaaa acacaaacaa tgtagaatga
     2101 cactgtgttt tttttgtaaa caaatgttgt ttttggttaa acatctttgt gaaaacatcc
     2161 tcctgtcatg tatttgctat attcaaatgt taaacccgtg cagaatagaa catatacaaa
     2221 aaaaaacaac acaacacatt tttaaacatt attaaatatc aagtattgct ggcagttctg
     2281 tttctgtttt acagtaccct ttgccacagt tctccgcttt tcctggtcca gattccacaa
     2341 gtctgattca ccaatagcaa agcgaataaa caaccaaagc agccaatcac tgcttgtaga
     2401 ctgtcctgcg agaccggccc attccagcac attctggaaa cttcctttat atgataatta
     2461 taaatacatt taaattattg atacaaaaca tgtaattcct agaacataac catagcaatc
     2521 attagttttc agggtaatta tgtattttta ggatttgact gcggaaagat ctggtcatgt
     2581 gacgtctcat gaacgtcacg gccctgggtt tctataaata cagtaggact ctcgaccatc
     2641 ggcagatttt tcgaagaaga agatcagttt caggagccgt actgttccgt ttcaacgcaa
     2701 atattaacgg taagagcgaa tttcctagtt tgttttcatg ccattcttta aaaccatagc
     2761 gtattacttt aattatagta aactttcgct ttctttatta caagagacgt tttgtgttga
     2821 ttctccgcgg acattttcgg tcagacaatc agaaaatgac cgcggaggac cagtaacttg
     2881 cattacacgt aagttaaatc ttcgtgtatt aaaatggtta ggttgttaac gtcaaatagg
     2941 ttaccgtgtt tgcgtgtgat caggttggtt ttgttagatt tttgtcagta tttttaattt
     3001 atttgtttta gtttatttat tttttttgct gaatcatagt ttgtgaacaa agaacccgga
     3061 tgttacatac agtacagccg ccatgttaca gagagttata acttaatcat tttaaaaata
     3121 attttgcctt acttttagtt tgtcatgttg agaaatgagg aaatgttaaa atgaggaaat
     3181 atccaattaa tttaatatat caaaataatc catgattaca atgcactgaa ctggagaaaa
     3241 ttaagatgtt ttctagtgtc atgaaacaaa tgtaagagat gtacattgta gatgttttat
     3301 gtcaagaatt ggctagttga tgcagcatac tggcgatact cagttgtaat aacagtaacg
     3361 ttacatgtta atagactact gagtatgctg ttctgtctat gtatgctctg taagctacga
     3421 gaaggacttt tttaaacagt aaagggtgca atatttttac aaattgaatt aaataaaggc
     3481 tgtctattaa gtaatatgct tgatattttt cttacttgat cggaaataag aaaaaatata
     3541 aacgttgttg ctctaaaaat cctagttcag tttagccaac cacaaatacc tttttgttcc
     3601 tccaacagtt tttttttctt ctctataata tttggcagtc tatagtactc caaatgtttc
     3661 cccacagtct aactaattgg tacagccaaa atcatgacac ttattgcaat aataattttg
     3721 gttcattggc attgttgata gcctgtgcca ctaatatggt cgattgatca tgcttcagga
     3781 agaaaactat attgtttgat gtaagattat taaatcttca cctgcttcca ttacaaacta
     3841 ttcccatctt attgaattct ggtatgtctt aaaggattag ttcacttccc aaatcaaaat
     3901 ttacttagcc ttttttcatc catgatccct ttttttcatc attaatgaag aaattgtttt
     3961 tgaaaaagtt tcaagatttt tttctctata ttgtggagct tgttagttta aaattccaaa
     4021 atgcaatatg tggcttcaaa tggttctaaa tgatcccagt caaggaataa cagtcttatc
     4081 taatgaaacc attagacctt taaaaataaa aaaaataaaa gtatttattt ttaaatgact
     4141 gagtgattaa gttgaatttc agcgtttcct tactgtgtag aagtccttcc ttactggccc
     4201 caccctttgg ttctctgcca atctgctacc taatgtaatg ttgtggaaca ttattattct
     4261 ttatttctta attttttatt ttttatttta aaacaatgta aactgcacag atgtgcagtt
     4321 tgtttaaaat ggccaatgct ttggaaatgc atgacataat tagatttcat gatgcacaaa
     4381 gccaaatctc agagcttgtg caaaatgagc tatcatttca ctaggtaaga ccctaaattt
     4441 tcatatagga tcatttggac aattttgctg caggtaaaat gcattctata gtccactgtc
     4501 agccattgtt ttggatagta tttatttttc tctacaagta tagtcaatag ttttctatta
     4561 ttttaaaggt ttgtaacatt taagggtgac caaatgcaaa gtaaaatttc attttcgggt
     4621 gaactatctc gtttaacatg ggagaagtgc aaaacataca ttattggcta gaacattgta
     4681 gtatttttta aatggaaatg tgtgattgct aatcttactt tgaatttgtt tacagggatc
     4741 ctttaagggc gaattcgacc caagtttgta caaaaaagca ggctcgaatt ggagctcgcc
     4801 gccaccatgg cacccaagaa gaagaggaag atgccgccta aacgcaagac actcaatgcc
     4861 gctgccgaag ccaatgccca cgctgatggc catgctgatg gcaatgctga tggtcatgtc
     4921 gctaacactg cagcaagcag caacaacgcc cgttttgcgg acttgaccaa cattgacaca
     4981 cccggcctcg gccctaccac gacgacgtta cttgtcgagc ccgctcgttc gaaacgccag
     5041 agagtctcga gggcctgtga tcagtgtcga gctgcacgtg aaaagtgtga tggaatccag
     5101 ccggcttgct tcccctgtgt gtcgcagggc cggtcgtgta cctaccaggc cagtcccaag
     5161 aagcgaggag tccagacggg ctacatccgc actctcgaac tggctctggc ttggatgttc
     5221 gagaacgttg cccgcagcga ggacgccctc cacaatcttt tggtccgtga tgctggccag
     5281 ggcagcgctc tcctggtcgg caaagactcg cctgctgcag aacgcctgca tgcaagatgg
     5341 gcgacgagtc gagtcaacaa aagcatcacc cgtcttctct caggtgctag cactagtagc
     5401 gccggtcaac agcaacagct gcaatcatta gtgccactga ccccgtctgc tttgtttggt
     5461 ggcgccaatt ttaatcaaag tgggaatatt gctgatagct cattgtcctt cactttcact
     5521 aacagtagca acggtccgaa cctcataaca actcaaacaa attctcaagc gctttcacaa
     5581 ccaattgcct cctctaacgt tcatgataac ttcatgaata atgaaatcac ggctagtaaa
     5641 attgatgatg gtaataattc aaaaccactg tcacctggtt ggacggacca aactgcgtat
     5701 aacgcgtttg gaatcactac agggatgttt aataccacta caatggatga tgtatataac
     5761 tatctattcg atgatgaaga taccccacca aacccaaaaa aagagtaacc cgggagcggc
     5821 ccctctcgag cctctagaac tatagtgagt cgtattacgt agatccagac atgataagat
     5881 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     5941 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagagatct
     6001 gatctagagg atcaagctta tcgataccgt cgacctcgag ggggggcccg gtacccagct
     6061 tttgacccag ctttcttgta caaagtggga agatctaccg gtgagctcca ccgcggtggc
     6121 ggccgctcta gaactagtgg atcccccggg ctgcaggaat tcgatatcaa gcttatcgat
     6181 accgtcgacc tcgagggggg gcccggtacc gcaactttat tatacatagt tgataattca
     6241 ctggccgtcg ttttacggta ccatcgatga tgatccagac atgataagat acattgatga
     6301 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     6361 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     6421 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
     6481 cctctacaaa tgtggtatgg ctgattatga tcctctagat cagatcttga ccaaagctta
     6541 aatcagttgt gttaaataag agacattcaa aataaatgta aatgagctct ccaaatcagc
     6601 agacttaaca ttctttaaaa tgattgattc aatagtgata aaaatcaggc atagccagtt
     6661 gtaactttag ataaattaca gaaaatgtca aatacagaga accgattctt ttttatgata
     6721 catccaagca cacatttaac acaatccagg caaaccccga atttcacagt cacaagcact
     6781 gtttgtacaa gagctttgcc taaggacaca cagtctctat aagtccaggt cgttggtttc
     6841 actcttattt taaacatgtg acatttttcc tgccatcctg tcttaggctg ctgtttgctt
     6901 cattccatgt cacattaatt tcctcagtag caccttttac acacacagcc aatcttttcc
     6961 agaaaattca attgctttga agagataatg tgtgaacaaa tccatttaga aaaggaaaat
     7021 taagaatttg taaaatcatc tgtaaattgt tggcattctt ctgtatatga acatcacatc
     7081 atttacaggt aaaggtctgg tcattaatta tatgacaatt tactggtatt attttgtgaa
     7141 aggggctatt ttcaatgcgt tcatccatcc ttttcatccc tcaaatctct cattcacgtc
     7201 cccctcccca tctgcacact ttatctcatt ttccaccctg ctggaatctg agcacttgtg
     7261 cagttatcag ggctcctgta tttaggaggc tctgggtgtc catgtagggg acgaacagaa
     7321 acactgcaga cctttataga agaacaaatg ataagagtcc tcgtacataa agactccatt
     7381 agtaacgtca gtgacccagg agcccagacc aacagcaaag cagacagtga aatcactagt
     7441 gaattcgcgg ccgcctgcag gtcgacggta tcgattcgaa ttcaaggcct ctcgagcctc
     7501 taagccacca tgggctgcat caagagcaag cgcaaggaca acctgaacga cgacgaggcc
     7561 gccatgggct gcatcaagag caagcgcaag gacaacctga acgacgacga ggcaccggtc
     7621 gccaccatgg cctcctccga ggacgtcatc aaggagttca tgcgcttcaa ggtgcgcatg
     7681 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
     7741 gagggcaccc agaccgccaa gctgaaggtg accaagggcg gccccctgcc cttcgcctgg
     7801 gacatcctgt cccctcagtt ccagtacggc tccaaggcct acgtgaagca ccccgccgac
     7861 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
     7921 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc
     7981 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag
     8041 aagaccatgg gctgggaggc ctccaccgag cggatgtacc ccgaggacgg cgccctgaag
     8101 ggcgagatca agatgaggct gaagctgaag gacggcggcc actacgacgc cgaggtcaag
     8161 accacctaca tggccaagaa gcccgtgcag ctgcccggcg cctacaagac cgacatcaag
     8221 ctggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga gcgcgccgag
     8281 ggccgccact ccaccggcgc ctaatacgta gatccagaca tgataagata cattgatgag
     8341 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     8401 gctattgctt tatttgtaac cattataagc tgcaataaac aagagatctg cgaagatacg
     8461 gccacgggtg ctcttgatcc tgtggctgat tttggactgt gctgctcgca gctgctgatg
     8521 aatcacatac ttcctccatt ttcttccact gattgactgt tataatttcc ctaatttcca
     8581 ggtcaaggtg ctgtgcattg tggtaataga tgtgacatga cgtcacttcc aaaggaccaa
     8641 tgaacatgtc tgaccaattt catataatgt gaaaacgatt ttcataggca gaataaataa
     8701 catttaaatt aaactgggca tcagcgcaat tcaattggtt tggtaatagc aagggaaaat
     8761 agaatgaagt gatctccaaa aaataagtac tttttgactg taaataaaat tgtaaggagt
     8821 aaaaagtact tttttttcta aaaaaatgta attaagtaaa agtaaaagta ttgattttta
     8881 attgtactca agtaaagtaa aaatccccaa aaataatact taagtacagt aatcaagtaa
     8941 aattactcaa gtactttaca cctctggttc ttgaccccct accttcagca agcccagcag
     9001 atccactagt tctagagcgg ccgccaccgc ggtggagctc cagcttttgt tccctttagt
     9061 gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     9121 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     9181 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     9241 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     9301 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     9361 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     9421 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     9481 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     9541 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     9601 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     9661 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     9721 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     9781 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     9841 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     9901 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     9961 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
    10021 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
    10081 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
    10141 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
    10201 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
    10261 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
    10321 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
    10381 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
    10441 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
    10501 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
    10561 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
    10621 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
    10681 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
    10741 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
    10801 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
    10861 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    10921 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    10981 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    11041 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    11101 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    11161 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    11221 catttccccg aaaagtg
//
