LOCUS       Exported               11753 bp ds-DNA     circular SYN 29-JUN-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jun 29, 2020 from SnapGene Viewer 5.1.4.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11753
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1169..1189
                     /label=M13-rev
     misc_recomb     complement(1205..1225)
                     /label=attB4
     misc_feature    1226..5200
                     /label=acta1 promoter
     misc_feature    5280..5288
                     /label=Kozak
     misc_feature    5289..5312
                     /label=NLS
     misc_feature    5313..5867
                     /label=QFDBD
     misc_feature    5889..6290
                     /label=Gal4AD
     misc_feature    5945..6287
                     /label=Gal4 activation domain
     polyA_signal    6324..6515
                     /label=SV40 late polyA
     polyA_signal    6792..6983
                     /label=SV40 late polyA
     misc_feature    7044..7947
                     /label=cmlc2 promoter
     misc_feature    8026..8142
                     /label=membrane targeting sequence
     CDS             8146..8817
                     /label=mRFP1
     misc_feature    8827..8959
                     /label=SV40 polyA
     primer_bind     complement(9567..9586)
                     /label=T3
     primer_bind     complement(9604..9624)
                     /label=M13-rev
     misc_binding    complement(9630..9652)
                     /label=LacO
     promoter        complement(9657..9686)
                     /label=lac
     rep_origin      complement(9992..10620)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(10772..11431)
                     /label=AmpR
     promoter        complement(11671..11699)
                     /label=Amp prom
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccgggctg gctaagaact catcagcctc
      361 cccggtccat ctacccacgt accaatgcac caattggcca caatgacggc tactacatgg
      421 tgccattcct tcctctttat aggaatggag actacctcct gtccaacaag gctcttggat
      481 acgagtacgc ctacctgttg gacccaggtc attgcacaac accagaaatg ccctctgatc
      541 tgcaaaagac gtgaatatct gttcagacac ccatatccac tctgttccac acaggtcaga
      601 ggtttgtcca ggagttcttg acagaggtgt aaaaagtact caaaaatttt actcaagtga
      661 aagtacaagt acttagggaa aattttactc aattaaaagt aaaagtatct ggctagaatc
      721 ttacttgagt aaaagtaaaa aagtactcca ttaaaattgt acttgagtat taaggaagta
      781 aaagtaaaag caagaaagaa aactagagat tcttgtttaa gcttttaatc tcaaaaaaca
      841 ttaaatgaaa tgcatacaag gttttatcct gctttagaac tgtttgtatt taattatcaa
      901 actataagac agacaatcta atgccagtac acgctactca aagttgtaaa acctcagatt
      961 taacttcagt agaagctgat tctcaaaatt gttagtgtca agcctagctc ttttggggct
     1021 gaaaagcaat cctgcagtgc tgaaaagcct ctcacaggca gccgatgcgg gaagaggtgt
     1081 attagtcttg atagagaggc tgcaaatagc aggaaacgtg agcagagact ccctggtgtc
     1141 tgaaacacag gccagatggg ccctcgagca ggaaacagct atgaccatga ttacgccaag
     1201 ctatcaactt tgtatagaaa agttggcacg cgcagattcc gtgtgggcct ggtaatatct
     1261 taccatttta agctatttta agattaggga aaaaaattaa ataaaactac tttaaaatta
     1321 ctttccttaa aaagcaacat atttatttag tttttgttta actggctcaa gattgtaata
     1381 ttttactttt acatgttttt tttaagtacc taaatgtgtt caaaagtaaa gtatgttatt
     1441 gttttttttt ttttaatgtt agctaagctt taaattgtaa tatattactt ttaaaagtga
     1501 ctttttaaaa atgtaaataa aaaagtaact ataaatgtgt aatgcatgtt atttctgtgg
     1561 gttttttaat tttcatctca actgggctta caattttaac ataataaaaa tcaaatcaaa
     1621 tcaaatcagg cccttatttg tcacatacac tcttgtatac tgaaataaac taaatgttga
     1681 gaaaaattta aatacaccaa aataacgcat accttttttg taattaccta agtaaaacat
     1741 ttagttcctt ttttaagtga ctcaagattg taatctatta attgtaaaag ataaaactag
     1801 tgatcaatct gacgtgcttc tatgcagcct gtgagattaa ttgtgatgtt taattattaa
     1861 acaatttaaa tggatcaaac atttcacatg tgcttattgt gtccaacaaa accagcagaa
     1921 gcacttaaaa atattgcatg acaacatgag tttttaatgt aaattatata tacttcctaa
     1981 acgaagttaa tcatattttc taaaataata atgtcattga aatatttaaa taaatgataa
     2041 nctttagttg attcaacata aaattgaaag caataaggac tttttttttt ttacagcggt
     2101 gaagcaaaag cntgnaaata atcatataaa tataataaat aaataaataa aataatggtt
     2161 cttggtgcaa atatgaacac tttaaataaa tatagaataa atatgatcaa taagtcataa
     2221 cgacgtgttt tatgttgatt ataatttaaa catgtttcta acgcaagttt taaaaactgt
     2281 tttaaagtat tattttaagt tataagatca atttgactca acattttgta gtgaaaatat
     2341 gttgtgtaaa attaatataa tatacactat aaaaatgcaa ggttccacac aattcattca
     2401 tgttgtccca acacaaatcg attaagttaa caaatatatg tggattgaac ataaaaaatt
     2461 aagttgtccc aatgaaatct caagaattgt gttgtttcag ctcattttaa ataagtagtt
     2521 ttaacaagta aaaaataaca tatttttgag tgtgtatata tatatattta tatattacaa
     2581 aatataataa tataaaatgt aaaagatata aaccatgcta catatattta agagctattt
     2641 tgcacattac ttttttgcaa gccatttgag tgttttttta caaatatata ttagctaaat
     2701 atacaatatc ttaaatatac aattaatcag acatgcaaaa aatgcattta acctacataa
     2761 aaatacatga tcaacatatg ttttatgctg attacaacta aatcaaacgt aatgaaatta
     2821 atttataaaa gcgatttaaa tatgtcaata ttttaagtta aaatcacttg aaacctacta
     2881 taaatgaact taaatttaaa aaatctcctt taattccaat gcagcactgt tgttttttca
     2941 gctgaggacc cccggtgcag atggttggcc cccggcccgc tattctaagg aagcctttct
     3001 taattttagg gcggcgctgc atgcgctcag atcagattca cgggtggaga gggttatttt
     3061 ctctttttta ttttttcttg cctgctcata tttgggcctg tgtgaacccc aggctccgcc
     3121 tcggccccca ggagccgctc ttatttaaag gggatcctcg ccttcagtct tcgtcccagt
     3181 ggaagcatca gtcttgtgat aacaacttgg tgagtcctac agaccagcgc tcgactctag
     3241 gctttaaatg cattgatttc tctggattag ctaccgggag agttttaact ttgcttatta
     3301 gtcttgtgct tgaactgcta ggtttaaaga gggttttctc taatgcatgc atgaagcgac
     3361 agctgaaaac cagaaataga gcgtttgtcc tcctgttaaa ctctagcctt tctttggcat
     3421 ttgtcagtcc gagtcttcag ctgccggcag gagattgggt ttcagtaaca cttgctgagc
     3481 acaaactaca cgtgcttcct tcagatttaa ttcattatta atcattctag atttaaatga
     3541 tcttcttact cattgctgat tattttagcc ttgcggagca gattaaatga gcacgtgttt
     3601 attaagattg atttaaatat aattaatatt tcaccactgt agttttaaaa ttgccttcta
     3661 agtcatggtt gatttttaat aggtatcggc tctaattaga ttactatttt ggtttttaaa
     3721 tgttatttct atttcatttg taggctcgtt tttaatgtga ttttattaga ttctagtttt
     3781 aaaatcatct tttagcctta ctgagcagac tgaatgaaca tgtttcatta agattaactt
     3841 cattatagtt aattatttaa tcattctagt ggattataaa tcagctctat ttaaattgat
     3901 tgtcgtgttt atttctattt gatttttgat tattttttaa ggtcgttttt atgtgatttt
     3961 tccccgattc taattttaaa ctaattgttt gatttttata tgattcgact ctttttaaat
     4021 gaattgtttt gcttttttta ttgagcatat atatatatta tacaatttca atcaacaatt
     4081 gttgtgcttt ttaaatgttg tttctatttc atttctaggg tcgattttat tcgattctag
     4141 ttttaaaatc gtgatttcta actcctaacc ttactgagca gactgaatga acatgtttca
     4201 tcaagattaa cttcattata gttaattatt gaatcgggct agtggtttat aaatcagctc
     4261 tattataaat taattgtcat cattatttct atttaatttt tattattttt ttaggattat
     4321 ttttaaggga tttttttcac gttctagttt taaactcaat gctcattact gctcaccctt
     4381 gctgagcagt ctttcgttaa gattaaatta attaataacc cattctagtt ttaaaacagt
     4441 gtcttaaggc atagctgata ttttacaagt ttactattta atatatctcc atttcttttt
     4501 ttagggtcga tttcatctga tttgattaat ttttattatc ttcactaagc tttttgttct
     4561 aggttttatt ctcagtatta ttactgttat atttgaactg aatttctcct catagtttat
     4621 tcattttatt tatttatttt ttgtttattc gctattttgt attttagttt ttaattagaa
     4681 attttattag aatcgttttg aactagtttt tatgcactct aacattaata ttaaatcata
     4741 actattattt ttattataat ttatttgtgt ttatcttttt gtgtagcttt catgtgtttt
     4801 tattttagtt ttattatgtt tgcattgaat tttttggact agtttatata acgatataca
     4861 gaaaagccat gacaacaaat gtttttataa tacctattaa gtataagcta atcagatttc
     4921 cactattctg ttcaaagtta acattattag ccattctgat ggatcatttg acatttgtca
     4981 tatgtcttta tgaacactgc tctttcagat gccactttta aagctgtttt ttacagtaaa
     5041 tgaacgttta ttattatgac attatttcct aaggcctcta gagtacagca ggatgtggcg
     5101 tgtgcattac tgtgtaatta tgctgactgt aaatgtgcat ttatagactg tcagcatgtc
     5161 tgactgtgcg ctgttgtgat ttgtcctgca cagaccaagg caagtttgta caaaaaagca
     5221 ggctcgaatt ggagctccac cgcggtggcg gccgctctag aactagtgga tcccccggtg
     5281 ccgccaccat ggcacccaag aagaagagga agatgccgcc taaacgcaag acactcaatg
     5341 ccgctgccga agccaatgcc cacgctgatg gccatgctga tggcaatgct gatggtcatg
     5401 tcgctaacac tgcagcaagc agcaacaacg cccgttttgc ggacttgacc aacattgaca
     5461 cacccggcct cggccctacc acgacgacgt tacttgtcga gcccgctcgt tcgaaacgcc
     5521 agagagtctc gagggcctgt gatcagtgtc gagctgcacg tgaaaagtgt gatggaatcc
     5581 agccggcttg cttcccctgt gtgtcgcagg gccggtcgtg tacctaccag gccagtccca
     5641 agaagcgagg agtccagacg ggctacatcc gcactctcga actggctctg gcttggatgt
     5701 tcgagaacgt tgcccgcagc gaggacgccc tccacaatct tttggtccgt gatgctggcc
     5761 agggcagcgc tctcctggtc ggcaaagact cgcctgctgc agaacgcctg catgcaagat
     5821 gggcgacgag tcgagtcaac aaaagcatca cccgtcttct ctcaggtgct agcactagta
     5881 gcgccggtca acagcaacag ctgcaatcat tagtgccact gaccccgtct gctttgtttg
     5941 gtggcgccaa ttttaatcaa agtgggaata ttgctgatag ctcattgtcc ttcactttca
     6001 ctaacagtag caacggtccg aacctcataa caactcaaac aaattctcaa gcgctttcac
     6061 aaccaattgc ctcctctaac gttcatgata acttcatgaa taatgaaatc acggctagta
     6121 aaattgatga tggtaataat tcaaaaccac tgtcacctgg ttggacggac caaactgcgt
     6181 ataacgcgtt tggaatcact acagggatgt ttaataccac tacaatggat gatgtatata
     6241 actatctatt cgatgatgaa gataccccac caaacccaaa aaaagagtaa gaattcacta
     6301 tagtgagtcg tattacgtag atccagacat gataagatac attgatgagt ttggacaaac
     6361 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     6421 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     6481 gtttcaggtt cagggggagg tgtgggaggt tttttaattc gcggccatca agcttatcga
     6541 taccgtcgac ctcgaggggg ggcccggtac ccagcttttg acccagcttt cttgtacaaa
     6601 gtgggaggta ccgggccccc cctcgaggtc gacggtatcg ataagcttga tatcgaattc
     6661 ctgcagcccg ggggatccac tagttctaga gcggccgcca ccgcggtgga gctcaccggt
     6721 agatctgcaa ctttattata catagttgat aattcactgg ccgtcgtttt acggtaccat
     6781 cgatgatgat ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     6841 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     6901 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
     6961 gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga
     7021 ttatgatcct ctagatcaga tcttgaccaa agcttaaatc agttgtgtta aataagagac
     7081 attcaaaata aatgtaaatg agctctccaa atcagcagac ttaacattct ttaaaatgat
     7141 tgattcaata gtgataaaaa tcaggcatag ccagttgtaa ctttagataa attacagaaa
     7201 atgtcaaata cagagaaccg attctttttt atgatacatc caagcacaca tttaacacaa
     7261 tccaggcaaa ccccgaattt cacagtcaca agcactgttt gtacaagagc tttgcctaag
     7321 gacacacagt ctctataagt ccaggtcgtt ggtttcactc ttattttaaa catgtgacat
     7381 ttttcctgcc atcctgtctt aggctgctgt ttgcttcatt ccatgtcaca ttaatttcct
     7441 cagtagcacc ttttacacac acagccaatc ttttccagaa aattcaattg ctttgaagag
     7501 ataatgtgtg aacaaatcca tttagaaaag gaaaattaag aatttgtaaa atcatctgta
     7561 aattgttggc attcttctgt atatgaacat cacatcattt acaggtaaag gtctggtcat
     7621 taattatatg acaatttact ggtattattt tgtgaaaggg gctattttca atgcgttcat
     7681 ccatcctttt catccctcaa atctctcatt cacgtccccc tccccatctg cacactttat
     7741 ctcattttcc accctgctgg aatctgagca cttgtgcagt tatcagggct cctgtattta
     7801 ggaggctctg ggtgtccatg taggggacga acagaaacac tgcagacctt tatagaagaa
     7861 caaatgataa gagtcctcgt acataaagac tccattagta acgtcagtga cccaggagcc
     7921 cagaccaaca gcaaagcaga cagtgaaatc actagtgaat tcgcggccgc ctgcaggtcg
     7981 acggtatcga ttcgaattca aggcctctcg agcctctaag ccaccatggg ctgcatcaag
     8041 agcaagcgca aggacaacct gaacgacgac gaggccgcca tgggctgcat caagagcaag
     8101 cgcaaggaca acctgaacga cgacgaggca ccggtcgcca ccatggcctc ctccgaggac
     8161 gtcatcaagg agttcatgcg cttcaaggtg cgcatggagg gctccgtgaa cggccacgag
     8221 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
     8281 aaggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc tcagttccag
     8341 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
     8401 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
     8461 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
     8521 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
     8581 accgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagat gaggctgaag
     8641 ctgaaggacg gcggccacta cgacgccgag gtcaagacca cctacatggc caagaagccc
     8701 gtgcagctgc ccggcgccta caagaccgac atcaagctgg acatcacctc ccacaacgag
     8761 gactacacca tcgtggaaca gtacgagcgc gccgagggcc gccactccac cggcgcctaa
     8821 tacgtagatc cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     8881 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     8941 ataagctgca ataaacaaga gatctgcgaa gatacggcca cgggtgctct tgatcctgtg
     9001 gctgattttg gactgtgctg ctcgcagctg ctgatgaatc acatacttcc tccattttct
     9061 tccactgatt gactgttata atttccctaa tttccaggtc aaggtgctgt gcattgtggt
     9121 aatagatgtg acatgacgtc acttccaaag gaccaatgaa catgtctgac caatttcata
     9181 taatgtgaaa acgattttca taggcagaat aaataacatt taaattaaac tgggcatcag
     9241 cgcaattcaa ttggtttggt aatagcaagg gaaaatagaa tgaagtgatc tccaaaaaat
     9301 aagtactttt tgactgtaaa taaaattgta aggagtaaaa agtacttttt tttctaaaaa
     9361 aatgtaatta agtaaaagta aaagtattga tttttaattg tactcaagta aagtaaaaat
     9421 ccccaaaaat aatacttaag tacagtaatc aagtaaaatt actcaagtac tttacacctc
     9481 tggttcttga ccccctacct tcagcaagcc cagcagatcc actagttcta gagcggccgc
     9541 caccgcggtg gagctccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt
     9601 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     9661 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     9721 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     9781 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     9841 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     9901 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     9961 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
    10021 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
    10081 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
    10141 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
    10201 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
    10261 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
    10321 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
    10381 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
    10441 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
    10501 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
    10561 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
    10621 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
    10681 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
    10741 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
    10801 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
    10861 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
    10921 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
    10981 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
    11041 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
    11101 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
    11161 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
    11221 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
    11281 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
    11341 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
    11401 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
    11461 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
    11521 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    11581 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
    11641 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
    11701 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtg
//
