LOCUS       Exported                7869 bp ds-DNA     circular SYN 29-JUN-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jun 29, 2020 from SnapGene Viewer 5.1.4.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7869
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1169..1189
                     /label=M13-rev
     misc_recomb     complement(1205..1225)
                     /label=attB4
     misc_feature    1408..1423
                     /label=qa-1F
     misc_feature    1428..1443
                     /label=qa-2
     misc_feature    1448..1463
                     /label=qa-1F
     misc_feature    1468..1483
                     /label=qa-2
     misc_feature    1488..1503
                     /label=qa-1F
     misc_feature    1506..1576
                     /label=E1b
     misc_feature    1629..1637
                     /label=Kozak
     misc_feature    1638..2483
                     /label=GFPnls
     CDS             1641..2354
                     /label=EGFP
     misc_feature    2355..2483
                     /label=StingerNLS
     misc_feature    2517..2645
                     /label=SV40
     polyA_signal    2908..3099
                     /label=SV40 late polyA
     misc_feature    3160..4063
                     /label=cmlc2 promoter
     misc_feature    4142..4258
                     /label=Membrane Targeting Seq
     CDS             4262..4933
                     /label=mRFP1
     misc_feature    4943..5075
                     /label=SV40 short polyA
     primer_bind     complement(5683..5702)
                     /label=T3
     primer_bind     complement(5720..5740)
                     /label=M13-rev
     misc_binding    complement(5746..5768)
                     /label=LacO
     promoter        complement(5773..5802)
                     /label=lac
     rep_origin      complement(6108..6736)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(6888..7547)
                     /label=AmpR
     promoter        complement(7787..7815)
                     /label=Amp prom
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccgggctg gctaagaact catcagcctc
      361 cccggtccat ctacccacgt accaatgcac caattggcca caatgacggc tactacatgg
      421 tgccattcct tcctctttat aggaatggag actacctcct gtccaacaag gctcttggat
      481 acgagtacgc ctacctgttg gacccaggtc attgcacaac accagaaatg ccctctgatc
      541 tgcaaaagac gtgaatatct gttcagacac ccatatccac tctgttccac acaggtcaga
      601 ggtttgtcca ggagttcttg acagaggtgt aaaaagtact caaaaatttt actcaagtga
      661 aagtacaagt acttagggaa aattttactc aattaaaagt aaaagtatct ggctagaatc
      721 ttacttgagt aaaagtaaaa aagtactcca ttaaaattgt acttgagtat taaggaagta
      781 aaagtaaaag caagaaagaa aactagagat tcttgtttaa gcttttaatc tcaaaaaaca
      841 ttaaatgaaa tgcatacaag gttttatcct gctttagaac tgtttgtatt taattatcaa
      901 actataagac agacaatcta atgccagtac acgctactca aagttgtaaa acctcagatt
      961 taacttcagt agaagctgat tctcaaaatt gttagtgtca agcctagctc ttttggggct
     1021 gaaaagcaat cctgcagtgc tgaaaagcct ctcacaggca gccgatgcgg gaagaggtgt
     1081 attagtcttg atagagaggc tgcaaatagc aggaaacgtg agcagagact ccctggtgtc
     1141 tgaaacacag gccagatggg ccctcgagca ggaaacagct atgaccatga ttacgccaag
     1201 ctatcaactt tgtatagaaa agttgcaaaa gctgggtacc gggccccccc tcgaggtcga
     1261 cggtatcgat aagcttgata tcgaattcct gcagcccggg ggatccacta gttctagagc
     1321 ggccgccacc gcggtggagc tccaattcgc caagtttgta caaaaaagca ggctcgaatt
     1381 ggagctccac cgcggtggcg gccgcaagga taaacaatta tcctcacggg taatcgctta
     1441 tcctcaagga taaacaatta tcctcacggg taatcgctta tcctcaagga taaacaatta
     1501 tcctcgcaag ggtcgactct agagggtata taatggatcc catcgcgtct cagcctcact
     1561 ttgagctcct ccacacgaat tccctcgacc tcgaagacgc gtctgtttgg gatccaccgg
     1621 tcactagtgc cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     1681 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     1741 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     1801 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
     1861 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     1921 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     1981 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     2041 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     2101 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     2161 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     2221 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
     2281 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     2341 acgagctgta caagagcagg cacagaaggc atcgccagcg ctctaggagc cgcaatcgca
     2401 gccgaagtcg cagcagtgaa cgaaaacgcc gtcaacggag ccgaagtcgc agcagtgaac
     2461 gaagacgcta cttgtacaag taatctagaa ctatagtgag tcgtattacg tagatccaga
     2521 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     2581 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     2641 acaagaagct tatcgatacc gtcgacctcg agggggggcc cggtacccag cttttgaccc
     2701 agctttcttg tacaaagtgg gaggtaccgg gccccccctc gaggtcgacg gtatcgataa
     2761 gcttgatatc gaattcctgc agcccggggg atccactagt tctagagcgg ccgccaccgc
     2821 ggtggagctc accggtagat ctgcaacttt attatacata gttgataatt cactggccgt
     2881 cgttttacgg taccatcgat gatgatccag acatgataag atacattgat gagtttggac
     2941 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     3001 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     3061 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
     3121 aatgtggtat ggctgattat gatcctctag atcagatctt gaccaaagct taaatcagtt
     3181 gtgttaaata agagacattc aaaataaatg taaatgagct ctccaaatca gcagacttaa
     3241 cattctttaa aatgattgat tcaatagtga taaaaatcag gcatagccag ttgtaacttt
     3301 agataaatta cagaaaatgt caaatacaga gaaccgattc ttttttatga tacatccaag
     3361 cacacattta acacaatcca ggcaaacccc gaatttcaca gtcacaagca ctgtttgtac
     3421 aagagctttg cctaaggaca cacagtctct ataagtccag gtcgttggtt tcactcttat
     3481 tttaaacatg tgacattttt cctgccatcc tgtcttaggc tgctgtttgc ttcattccat
     3541 gtcacattaa tttcctcagt agcacctttt acacacacag ccaatctttt ccagaaaatt
     3601 caattgcttt gaagagataa tgtgtgaaca aatccattta gaaaaggaaa attaagaatt
     3661 tgtaaaatca tctgtaaatt gttggcattc ttctgtatat gaacatcaca tcatttacag
     3721 gtaaaggtct ggtcattaat tatatgacaa tttactggta ttattttgtg aaaggggcta
     3781 ttttcaatgc gttcatccat ccttttcatc cctcaaatct ctcattcacg tccccctccc
     3841 catctgcaca ctttatctca ttttccaccc tgctggaatc tgagcacttg tgcagttatc
     3901 agggctcctg tatttaggag gctctgggtg tccatgtagg ggacgaacag aaacactgca
     3961 gacctttata gaagaacaaa tgataagagt cctcgtacat aaagactcca ttagtaacgt
     4021 cagtgaccca ggagcccaga ccaacagcaa agcagacagt gaaatcacta gtgaattcgc
     4081 ggccgcctgc aggtcgacgg tatcgattcg aattcaaggc ctctcgagcc tctaagccac
     4141 catgggctgc atcaagagca agcgcaagga caacctgaac gacgacgagg ccgccatggg
     4201 ctgcatcaag agcaagcgca aggacaacct gaacgacgac gaggcaccgg tcgccaccat
     4261 ggcctcctcc gaggacgtca tcaaggagtt catgcgcttc aaggtgcgca tggagggctc
     4321 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     4381 ccagaccgcc aagctgaagg tgaccaaggg cggccccctg cccttcgcct gggacatcct
     4441 gtcccctcag ttccagtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
     4501 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     4561 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
     4621 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
     4681 gggctgggag gcctccaccg agcggatgta ccccgaggac ggcgccctga agggcgagat
     4741 caagatgagg ctgaagctga aggacggcgg ccactacgac gccgaggtca agaccaccta
     4801 catggccaag aagcccgtgc agctgcccgg cgcctacaag accgacatca agctggacat
     4861 cacctcccac aacgaggact acaccatcgt ggaacagtac gagcgcgccg agggccgcca
     4921 ctccaccggc gcctaatacg tagatccaga catgataaga tacattgatg agtttggaca
     4981 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     5041 tttatttgta accattataa gctgcaataa acaagagatc tgcgaagata cggccacggg
     5101 tgctcttgat cctgtggctg attttggact gtgctgctcg cagctgctga tgaatcacat
     5161 acttcctcca ttttcttcca ctgattgact gttataattt ccctaatttc caggtcaagg
     5221 tgctgtgcat tgtggtaata gatgtgacat gacgtcactt ccaaaggacc aatgaacatg
     5281 tctgaccaat ttcatataat gtgaaaacga ttttcatagg cagaataaat aacatttaaa
     5341 ttaaactggg catcagcgca attcaattgg tttggtaata gcaagggaaa atagaatgaa
     5401 gtgatctcca aaaaataagt actttttgac tgtaaataaa attgtaagga gtaaaaagta
     5461 cttttttttc taaaaaaatg taattaagta aaagtaaaag tattgatttt taattgtact
     5521 caagtaaagt aaaaatcccc aaaaataata cttaagtaca gtaatcaagt aaaattactc
     5581 aagtacttta cacctctggt tcttgacccc ctaccttcag caagcccagc agatccacta
     5641 gttctagagc ggccgccacc gcggtggagc tccagctttt gttcccttta gtgagggtta
     5701 attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     5761 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     5821 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     5881 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     5941 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     6001 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     6061 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     6121 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     6181 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     6241 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     6301 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     6361 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     6421 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     6481 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     6541 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     6601 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     6661 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     6721 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     6781 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     6841 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     6901 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     6961 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     7021 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     7081 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     7141 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     7201 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     7261 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     7321 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     7381 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     7441 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     7501 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     7561 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     7621 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     7681 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     7741 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     7801 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     7861 cgaaaagtg
//
