LOCUS       Exported                7848 bp ds-DNA     circular SYN 29-JUN-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jun 29, 2020 from SnapGene Viewer 5.1.4.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7848
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1169..1189
                     /locus_tag="M13-rev(1)"
                     /label=M13-rev
     misc_recomb     complement(1205..1225)
                     /locus_tag="attB4"
                     /label=attB4
     misc_feature    1350..1374
                     /locus_tag="attB1"
                     /label=attB1
     misc_feature    1398..1405
                     /locus_tag="NotI"
                     /label=NotI
     misc_feature    1408..1423
                     /locus_tag="qa-1F"
                     /label=qa-1F
     misc_feature    1428..1443
                     /locus_tag="qa-2"
                     /label=qa-2
     misc_feature    1448..1463
                     /locus_tag="qa-1F(1)"
                     /label=qa-1F
     misc_feature    1468..1483
                     /locus_tag="qa-2(1)"
                     /label=qa-2
     misc_feature    1488..1503
                     /locus_tag="qa-1F(2)"
                     /label=qa-1F
     misc_feature    1506..1576
                     /locus_tag="E1b"
                     /label=E1b
     misc_feature    1597..1602
                     /locus_tag="MluI"
                     /label=MluI
     misc_feature    1629..1637
                     /locus_tag="Kozak"
                     /label=Kozak
     misc_feature    1638..2333
                     /locus_tag="mScarlet"
                     /label=mScarlet
     misc_feature    2334..2459
                     /locus_tag="StingerNLS"
                     /label=StingerNLS
     misc_feature    2460..2462
                     /locus_tag="Stop"
                     /label=Stop
     misc_feature    2496..2624
                     /locus_tag="SV40"
                     /label=SV40
     misc_feature    2676..2700
                     /locus_tag="attB2"
                     /label=attB2
     misc_feature    2823..2844
                     /locus_tag="attB3"
                     /label=attB3
     polyA_signal    2887..3078
                     /locus_tag="SV40 late polyA"
                     /label=SV40 late polyA
     misc_feature    3139..4042
                     /locus_tag="cmlc2 promoter"
                     /label=cmlc2 promoter
     misc_feature    4121..4237
                     /locus_tag="Membrane Targeting Seq"
                     /label=Membrane Targeting Seq
     CDS             4241..4912
                     /locus_tag="mRFP1"
                     /label=mRFP1
     misc_feature    4922..5054
                     /locus_tag="SV40 short polyA"
                     /label=SV40 short polyA
     primer_bind     complement(5662..5681)
                     /locus_tag="T3"
                     /label=T3
     primer_bind     complement(5699..5719)
                     /locus_tag="M13-rev"
                     /label=M13-rev
     misc_binding    complement(5725..5747)
                     /locus_tag="LacO"
                     /label=LacO
     promoter        complement(5752..5781)
                     /locus_tag="lac"
                     /label=lac promoter
     rep_origin      complement(6087..6715)
                     /direction=LEFT
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     CDS             complement(6867..7526)
                     /locus_tag="AmpR"
                     /label=AmpR
     promoter        complement(7766..7794)
                     /locus_tag="Amp prom"
                     /label=Amp prom promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccgggctg gctaagaact catcagcctc
      361 cccggtccat ctacccacgt accaatgcac caattggcca caatgacggc tactacatgg
      421 tgccattcct tcctctttat aggaatggag actacctcct gtccaacaag gctcttggat
      481 acgagtacgc ctacctgttg gacccaggtc attgcacaac accagaaatg ccctctgatc
      541 tgcaaaagac gtgaatatct gttcagacac ccatatccac tctgttccac acaggtcaga
      601 ggtttgtcca ggagttcttg acagaggtgt aaaaagtact caaaaatttt actcaagtga
      661 aagtacaagt acttagggaa aattttactc aattaaaagt aaaagtatct ggctagaatc
      721 ttacttgagt aaaagtaaaa aagtactcca ttaaaattgt acttgagtat taaggaagta
      781 aaagtaaaag caagaaagaa aactagagat tcttgtttaa gcttttaatc tcaaaaaaca
      841 ttaaatgaaa tgcatacaag gttttatcct gctttagaac tgtttgtatt taattatcaa
      901 actataagac agacaatcta atgccagtac acgctactca aagttgtaaa acctcagatt
      961 taacttcagt agaagctgat tctcaaaatt gttagtgtca agcctagctc ttttggggct
     1021 gaaaagcaat cctgcagtgc tgaaaagcct ctcacaggca gccgatgcgg gaagaggtgt
     1081 attagtcttg atagagaggc tgcaaatagc aggaaacgtg agcagagact ccctggtgtc
     1141 tgaaacacag gccagatggg ccctcgagca ggaaacagct atgaccatga ttacgccaag
     1201 ctatcaactt tgtatagaaa agttgcaaaa gctgggtacc gggccccccc tcgaggtcga
     1261 cggtatcgat aagcttgata tcgaattcct gcagcccggg ggatccacta gttctagagc
     1321 ggccgccacc gcggtggagc tccaattcgc caagtttgta caaaaaagca ggctcgaatt
     1381 ggagctccac cgcggtggcg gccgcaagga taaacaatta tcctcacggg taatcgctta
     1441 tcctcaagga taaacaatta tcctcacggg taatcgctta tcctcaagga taaacaatta
     1501 tcctcgcaag ggtcgactct agagggtata taatggatcc catcgcgtct cagcctcact
     1561 ttgagctcct ccacacgaat tccctcgacc tcgaagacgc gtctgtttgg gatccaccgg
     1621 tcactagtgc cgccaccatg gtgagcaagg gcgaggccgt gatcaaagaa ttcatgcgct
     1681 tcaaggtgca catggaaggc agcatgaacg gacacgagtt cgagatcgaa ggcgaaggcg
     1741 agggcagacc ttacgaggga acacagaccg ccaagctgaa agtgaccaaa ggcggacctc
     1801 tgcctttcag ctgggacatc ctgtctccac agtttatgta cggaagccgc gccttcacaa
     1861 agcaccctgc tgacatccct gactactaca agcagagctt ccctgaggga ttcaagtggg
     1921 agagagtgat gaacttcgag gacggcggag ccgtgacagt gacacaggat accagcctgg
     1981 aagatggaac cctgatctac aaagtgaagc tgcgcggaac aaacttccct cctgacggac
     2041 ctgtgatgca gaaaaagacc atgggatggg aagccagcac cgagagactg taccctgaag
     2101 atggcgtgct gaagggcgac atcaagatgg ccctgagact gaaagacggc ggaagatacc
     2161 tggccgactt caagaccacc tacaaggcca agaaacctgt gcagatgcct ggcgcctaca
     2221 acgtggacag aaagctggac atcaccagcc acaacgagga ctacaccgtg gtggaacagt
     2281 acgagagaag cgagggacgc cacagcacag gcggaatgga cgagctgtac aagagcaggc
     2341 acagaaggca tcgccagcgc tctaggagcc gcaatcgcag ccgaagtcgc agcagtgaac
     2401 gaaaacgccg tcaacggagc cgaagtcgca gcagtgaacg aagacgctac ttgtacaagt
     2461 aatctagaac tatagtgagt cgtattacgt agatccagac atgataagat acattgatga
     2521 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     2581 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagaagctt atcgataccg
     2641 tcgacctcga gggggggccc ggtacccagc ttttgaccca gctttcttgt acaaagtggg
     2701 aggtaccggg ccccccctcg aggtcgacgg tatcgataag cttgatatcg aattcctgca
     2761 gcccggggga tccactagtt ctagagcggc cgccaccgcg gtggagctca ccggtagatc
     2821 tgcaacttta ttatacatag ttgataattc actggccgtc gttttacggt accatcgatg
     2881 atgatccaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     2941 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     3001 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     3061 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg
     3121 atcctctaga tcagatcttg accaaagctt aaatcagttg tgttaaataa gagacattca
     3181 aaataaatgt aaatgagctc tccaaatcag cagacttaac attctttaaa atgattgatt
     3241 caatagtgat aaaaatcagg catagccagt tgtaacttta gataaattac agaaaatgtc
     3301 aaatacagag aaccgattct tttttatgat acatccaagc acacatttaa cacaatccag
     3361 gcaaaccccg aatttcacag tcacaagcac tgtttgtaca agagctttgc ctaaggacac
     3421 acagtctcta taagtccagg tcgttggttt cactcttatt ttaaacatgt gacatttttc
     3481 ctgccatcct gtcttaggct gctgtttgct tcattccatg tcacattaat ttcctcagta
     3541 gcacctttta cacacacagc caatcttttc cagaaaattc aattgctttg aagagataat
     3601 gtgtgaacaa atccatttag aaaaggaaaa ttaagaattt gtaaaatcat ctgtaaattg
     3661 ttggcattct tctgtatatg aacatcacat catttacagg taaaggtctg gtcattaatt
     3721 atatgacaat ttactggtat tattttgtga aaggggctat tttcaatgcg ttcatccatc
     3781 cttttcatcc ctcaaatctc tcattcacgt ccccctcccc atctgcacac tttatctcat
     3841 tttccaccct gctggaatct gagcacttgt gcagttatca gggctcctgt atttaggagg
     3901 ctctgggtgt ccatgtaggg gacgaacaga aacactgcag acctttatag aagaacaaat
     3961 gataagagtc ctcgtacata aagactccat tagtaacgtc agtgacccag gagcccagac
     4021 caacagcaaa gcagacagtg aaatcactag tgaattcgcg gccgcctgca ggtcgacggt
     4081 atcgattcga attcaaggcc tctcgagcct ctaagccacc atgggctgca tcaagagcaa
     4141 gcgcaaggac aacctgaacg acgacgaggc cgccatgggc tgcatcaaga gcaagcgcaa
     4201 ggacaacctg aacgacgacg aggcaccggt cgccaccatg gcctcctccg aggacgtcat
     4261 caaggagttc atgcgcttca aggtgcgcat ggagggctcc gtgaacggcc acgagttcga
     4321 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
     4381 gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tccagtacgg
     4441 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
     4501 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
     4561 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
     4621 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga
     4681 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagatgaggc tgaagctgaa
     4741 ggacggcggc cactacgacg ccgaggtcaa gaccacctac atggccaaga agcccgtgca
     4801 gctgcccggc gcctacaaga ccgacatcaa gctggacatc acctcccaca acgaggacta
     4861 caccatcgtg gaacagtacg agcgcgccga gggccgccac tccaccggcg cctaatacgt
     4921 agatccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     4981 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     5041 ctgcaataaa caagagatct gcgaagatac ggccacgggt gctcttgatc ctgtggctga
     5101 ttttggactg tgctgctcgc agctgctgat gaatcacata cttcctccat tttcttccac
     5161 tgattgactg ttataatttc cctaatttcc aggtcaaggt gctgtgcatt gtggtaatag
     5221 atgtgacatg acgtcacttc caaaggacca atgaacatgt ctgaccaatt tcatataatg
     5281 tgaaaacgat tttcataggc agaataaata acatttaaat taaactgggc atcagcgcaa
     5341 ttcaattggt ttggtaatag caagggaaaa tagaatgaag tgatctccaa aaaataagta
     5401 ctttttgact gtaaataaaa ttgtaaggag taaaaagtac ttttttttct aaaaaaatgt
     5461 aattaagtaa aagtaaaagt attgattttt aattgtactc aagtaaagta aaaatcccca
     5521 aaaataatac ttaagtacag taatcaagta aaattactca agtactttac acctctggtt
     5581 cttgaccccc taccttcagc aagcccagca gatccactag ttctagagcg gccgccaccg
     5641 cggtggagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca
     5701 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     5761 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     5821 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     5881 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     5941 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     6001 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     6061 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     6121 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     6181 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     6241 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     6301 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     6361 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     6421 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     6481 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     6541 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     6601 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     6661 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     6721 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     6781 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     6841 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     6901 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     6961 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     7021 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     7081 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     7141 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     7201 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     7261 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     7321 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     7381 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     7441 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     7501 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     7561 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     7621 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     7681 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     7741 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     7801 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtg
//
