LOCUS       Exported                6530 bp ds-DNA     circular SYN 17-JUN-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6530)
  AUTHORS   UNKNOWN
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jun 22, 2020 from SnapGene Viewer 5.1.3.1
            https://www.snapgene.com
COMMENT     ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..6530
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /label=AAV2\ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="/vntifkey=34"
                     /note="color: #ffff99"
     enhancer        168..296
                     /label=partial CMV enhancer
                     /label=partial\CMV\enhancer
                     /note="/vntifkey=9"
                     /note="color: #c0c0c0; direction: RIGHT"
     promoter        297..500
                     /label=CMV promoter
                     /label=CMV\promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="/vntifkey=30"
                     /note="color: #c0c0c0; direction: RIGHT"
     intron          542..634
                     /label=SV40 intron
                     /label=SV40\intron
                     /note="/vntifkey=15"
                     /note="color: #------"
     CDS             686..3574
                     /codon_start=1
                     /label=THBS4
                     /label=C-HA-Thbs4
                     /note="/vntifkey=4"
                     /note="color: #ff9900"
                     /translation="MPAPRAAAAAFLLLHLVLQPWQRTSAQATPQVFDLLPSSSQRLNP
                     SALQPVLTDPTLHEVYLISTFKLQSKSSATIFGLYSSSDNSKYFEFTVMGRLNKAILRY
                     LKNDGKIHLVVFNNLQLADGRRHRVLLRLSNLQRGDGSVELYLDCAQADSVRNLPRAFS
                     GLTQNPESIELRTFQRKPQDFLEELKLVVRGSLFQVASLQDCFLQQSEPLAATSTGDFN
                     RQFLGQMTQLNQLLGEVKDLLRQQVKETSFLRNTIAECQACGPLSFQSPTPNTLVPIAP
                     PAPPTRPTRHCDSSPCFRGVRCTDTRDGFQCGPCPDGYTGNGITCSDVDECKYHPCYPG
                     VRCVNLAPGFRCDACPVGFTGPMVQGVGINFAKTNKQVCTDVDECQNGACVLNSICINT
                     LGSYRCGPCKPGYTGDQTRGCKTERSCRNPEQNPCSVHAQCIEERQGDVTCVCGVGWAG
                     DGYVCGKDVDIDSYPDEELPCSARNCKKDNCKYVPNSGQEDADRDGIGDACDEDADGDG
                     ILNEQDNCVLTHNIDQRNSDKDIFGDACDNCRMVLNNDQKDTDGDGRGDACDDDMDGDG
                     IKNILDNCPRVPNRDQQDRDGDDVGDACDSCPDVSNPNQSDVDNDLVGDSCDTNQDSDG
                     DGHQDSTDNCPTVINSSQLDTDKDGIGDECDDDDDNDGIPDLVPPGPDNCRLVPNPAQE
                     DSNNDGVGDICEADFDQDQVIDHIDVCPENAEITLTDFRAYQTVVLDPEGDAQIDPNWV
                     VLNQGMEIVQTMNSDPGLAVGYTAFNGVDFEGTFHVNTQTDDDYAGFIFGYQDSSSFYV
                     VMWKQTEQTYWQATPFRAVAEPGIQLKAVKSKTGPGEHLRNSLWHTGDTSDQVRLLWKD
                     SRNVGWKDKVSYRWFLQHRPQVGYIRVRFYEGSELVADSGVTIDTTMRGGRLGVFCFSQ
                     ENIIWSNLKYRCNDTIPEDFQEFQTQSFDRLDK"
     CDS             3578..3604
                     /codon_start=1
                     /label=C-HA-Tag
                     /label=nonstandard type:  insertion_seq
                     /note="/vntifkey=14"
                     /note="color: #ff0000"
                     /translation="YPYDVPDYA"
     intron          3648..3781
                     /label=SV40 polyA
                     /label=SV40\polyA
                     /note="/vntifkey=15"
                     /note="color: #------"
     repeat_region   3793..3933
                     /label=AAV2 ITR
                     /label=AAV2\ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="/vntifkey=34"
                     /note="color: #ffff99"
     rep_origin      4008..4463
                     /direction=RIGHT
                     /label=f1 ori
                     /label=f1\ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="/vntifkey=33"
                     /note="color: #------"
     promoter        4745..4849
                     /gene="bla"
                     /label=AmpR promoter
                     /label=AmpR\promoter
                     /note="/vntifkey=30"
                     /note="color: #800000; direction: RIGHT"
     CDS             4850..5710
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="/vntifkey=4"
                     /note="color: #ff9966"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5881..6469
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="/vntifkey=33"
                     /note="color: #------"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgcc gctcgtgacc taggcatatg ccaagtacgc
      181 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      241 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      301 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      361 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      421 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      481 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccgg
      541 actctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg
      601 tttctctctt ttagattcca acctttggaa ctgaattccg cgggcccggg atcctctaga
      661 gtcgacctgc agaagctgag tcaccatgcc ggccccacgc gcggccgcgg ccgccttcct
      721 cctgctgcac ctggtcctgc agccgtggca gcggacgagc gcccaggcca caccccaggt
      781 ctttgatctt ctaccgtcct ccagccagag gctgaacccc agcgccctgc agccagtcct
      841 gacagatccc accctgcacg aagtctatct catctccacc ttcaagctgc agagtaaaag
      901 ctcagccacc atctttggcc tctactcctc aagtgacaac agcaaatact ttgaattcac
      961 tgtgatggga cggctgaaca aagccatcct ccgctacctg aagaatgatg gaaagatcca
     1021 tctggtggtg ttcaacaact tgcagctggc tgatgggagg cgccacaggg ttctcctgag
     1081 actgagcaat ttgcagagag gagatggctc tgtagaactc tacctggact gcgctcaggc
     1141 ggactcggtg cgcaacctcc ccagagcctt ttctggcctc acccagaacc cagagtccat
     1201 tgaattgcgg acattccaga ggaagcccca ggacttcttg gaagagctga agctggtggt
     1261 gcggggctcg ctgttccagg tggccagcct ccaagactgc ttcctgcagc agagtgagcc
     1321 tctggctgcc acaagcacag gagactttaa tcgacagttc ttgggtcaaa tgacacaact
     1381 gaaccagctc cttggagagg tgaaagatct tctgaggcag caggtcaagg aaacatcgtt
     1441 tttgcgaaac accattgctg aatgccaagc ttgtggtcct ctcagctttc agtccccaac
     1501 tccaaacaca ctggtgccca tagctcctcc agcaccccca actcgcccaa cacgccactg
     1561 tgactccagc ccttgtttcc gaggtgtccg atgtacggac accagggatg gcttccagtg
     1621 tggaccctgc ccagatggtt acacgggaaa cgggatcacc tgctctgacg tggatgagtg
     1681 caaataccat ccctgctatc caggtgtgcg ctgtgtgaat ttggctccgg gtttcagatg
     1741 tgacgcctgt ccagtaggtt tcacagggcc catggtgcag ggtgtcggga tcaactttgc
     1801 taaaacaaac aagcaggtgt gcactgatgt tgatgagtgt cagaatggag cgtgtgttct
     1861 gaattctatc tgcattaaca ctttgggatc ttatcgctgt gggccttgca aaccagggta
     1921 cactggtgat cagacgaggg gatgcaagac tgaaaggagc tgccggaacc cagagcagaa
     1981 cccatgcagt gtgcatgcgc agtgcattga ggagcggcaa ggggatgtga cgtgtgtgtg
     2041 tggtgttggc tgggctggtg acggctatgt ctgtggaaag gatgtggaca ttgacagtta
     2101 ccctgatgaa gaactgccat gctctgccag gaactgcaag aaggataact gcaagtatgt
     2161 gccaaactct ggccaggaag atgcagacag agatggcatc ggagacgcgt gtgatgagga
     2221 tgcagatgga gatgggatct tgaatgaaca ggacaactgt gtcctgactc acaatataga
     2281 ccaaaggaac agtgataaag atatctttgg ggatgcctgt gacaactgcc ggatggtcct
     2341 gaataatgac cagaaggata ctgacgggga tgggagagga gacgcctgtg atgacgacat
     2401 ggatggagat ggaataaaaa acattttgga caactgtccc agagttccca accgtgacca
     2461 gcaggatcgg gatggtgatg atgtcggaga tgcttgtgac agctgtcctg atgtcagtaa
     2521 ccctaaccag tcggatgtgg ataacgatct ggttggggac tcctgtgaca ccaaccaaga
     2581 cagtgacggg gatggccacc aggacagcac agacaactgc cccacagtca ttaacagctc
     2641 ccagctggac actgacaaag atgggatcgg cgatgaatgt gatgacgatg acgataacga
     2701 tggcatccca gacctggtgc ctcctggccc agacaactgc aggctcgtcc ccaacccagc
     2761 ccaggaagat agcaacaatg acggggtagg agatatctgt gaagctgact tcgaccagga
     2821 ccaggtcatc gatcacattg atgtgtgccc tgagaatgcg gagatcaccc tgactgactt
     2881 cagggcctac cagacagtgg tcctggaccc tgaaggggat gctcagatcg accccaactg
     2941 ggtggtcctg aaccagggca tggaaattgt gcagaccatg aacagtgacc ctggcctagc
     3001 tgttgggtac acggcattta acggagttga ctttgaaggg accttccacg taaacaccca
     3061 gacagatgat gactatgctg gctttatttt tggttaccaa gacagctcca gcttctatgt
     3121 agtcatgtgg aagcaaacgg agcagacata ctggcaggct acaccgttcc gtgcagtagc
     3181 agaacctggt attcagctca aggctgtgaa gtctaaaact ggcccagggg aacatctccg
     3241 aaactccctg tggcacacag gggacaccag cgaccaggtc aggctgctgt ggaaggactc
     3301 caggaatgtg ggctggaagg acaaggtctc ctaccgctgg ttcctgcagc ataggcccca
     3361 ggtgggctac atcagggtgc gcttttatga aggctcggag ttggtggctg actctggggt
     3421 caccatagac accaccatgc gtggaggccg acttggtgtg ttctgcttct ctcaagaaaa
     3481 catcatctgg tccaacctca agtaccgctg caacgacacc ataccagagg acttccagga
     3541 gttccagacc cagagtttcg accgcttgga taaagcttac ccatacgatg ttccagatta
     3601 cgcttagctc gagagatcta ggccgctagg cctcacctgc gatctcgatg ctttatttgt
     3661 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     3721 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     3781 actagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct
     3841 cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct
     3901 cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta
     3961 cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag
     4021 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     4081 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     4141 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     4201 cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata
     4261 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     4321 aactggaaca acactcaacc ctatctcggg ctattctttt gatttataag ggattttgcc
     4381 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     4441 caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc
     4501 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     4561 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     4621 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     4681 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     4741 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     4801 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     4861 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     4921 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     4981 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     5041 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     5101 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     5161 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     5221 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     5281 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     5341 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     5401 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     5461 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     5521 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     5581 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     5641 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     5701 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     5761 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     5821 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     5881 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     5941 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     6001 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     6061 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     6121 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     6181 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     6241 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     6301 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     6361 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     6421 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     6481 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
//
