LOCUS Exported 5706 bp ds-DNA circular SYN 22-JUN-2020 DEFINITION . ACCESSION . VERSION . KEYWORDS pAAV.CMV.CV40.THBS1-Delta-TSR3-CTD-HA.SV40(polyA) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5706) AUTHORS UNKNOWN TITLE Direct Submission JOURNAL Exported Monday, Jun 22, 2020 from SnapGene Viewer 5.1.3.1 https://www.snapgene.com COMMENT ORIGDB|GenBank FEATURES Location/Qualifiers source 1..5706 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 1..141 /label=AAV2 ITR /label=AAV2\ITR /note="inverted terminal repeat of adeno-associated virus serotype 2 vntifkey=34" /note="color: #ffff99" enhancer 168..296 /label=partial CMV enhancer /label=partial\CMV\enhancer /note="vntifkey=9" /note="color: #c0c0c0; direction: RIGHT" promoter 297..500 /label=CMV promoter /label=CMV\promoter /note="human cytomegalovirus (CMV) immediate early promoter vntifkey=30" /note="color: #c0c0c0; direction: RIGHT" intron 542..634 /label=SV40 intron /label=SV40\intron /note="vntifkey=15" /note="color: #------" CDS 678..2747 /codon_start=1 /label=THBS1-Delta-TSR3-CTD /label=Thbs1-C-HA-EGF_L1-3 /note="vntifkey=4" /note="color: #ff9900" /translation="MELLRGLGVLFLLHMCGSNRIPESGGDNGVFDIFELIGGARRGPG RRLVKGQDLSSPAFRIENANLIPAVPDDKFQDLLDAVWADKGFIFLASLRQMKKTRGTL LAVERKDNTGQIFSVVSNGKAGTLDLSLSLPGKQQVVSVEEALLATGQWKSITLFVQED RAQLYIDCDKMESAELDVPIQSIFTRDLASVARLRVAKGDVNDNFQGVLQNVRFVFGTT PEDILRNKGCSSSATNVLLTLDNNVVNGSSPAIRTNYIGHKTKDLQAICGLSCDELSSM VLELKGLRTIVTTLQDSIRKVTEENRELVSELKRPPLCFHNGVQYKNNEEWTVDSCTEC HCQNSVTICKKVSCPIMPCSNATVPDGECCPRCWPSDSADDGWSPWSEWTSCSATCGNG IQQRGRSCDSLNNRCEGSSVQTRTCHIQECDKRFKQDGGWSHWSPWSSCSVTCGDGVIT RIRLCNSPSPQMNGKPCEGEARETKACKKDACPINGGWGPWSPWDICSVTCGGGVQRRS RLCNNPTPQFGGKDCVGDVTENQVCNKQDCPIDGCLSNPCFAGAKCTSYPDGSWKCGAC PPGYSGNGIQCKDVDECKEVPDACFNHNGEHRCKNTDPGYNCLPCPPRFTGSQPFGRGV EHAMANKQVCKPRNPCTDGTHDCNKNAKCNYLGHYSDPMYRCECKPGYAGNGIIC" CDS 2754..2780 /codon_start=1 /label=HA-tag /label=C-HA-Tag /label=insertion_seq /note="vntifkey=14" /note="color: #ff0000" /translation="YPYDVPDYA" intron 2824..2957 /label=SV40 polyA /label=SV40\polyA /note="vntifkey=15" /note="color: #------" repeat_region 2969..3109 /label=AAV2 ITR /label=AAV2\ITR /note="inverted terminal repeat of adeno-associated virus serotype 2 vntifkey=34" /note="color: #ffff99" rep_origin 3184..3639 /direction=RIGHT /label=f1 ori /label=f1\ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis vntifkey=33" /note="color: #------" promoter 3921..4025 /gene="bla" /label=AmpR promoter /label=AmpR\promoter /note="vntifkey=30" /note="color: #800000; direction: RIGHT" CDS 4026..4886 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics vntifkey=4" /note="color: #ff9966" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 5057..5645 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication vntifkey=33" /note="color: #------" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgcc gctcgtgacc taggcatatg ccaagtacgc 181 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct 241 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga 301 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa 361 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc 421 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg 481 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccgg 541 actctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg 601 tttctctctt ttagattcca acctttggaa ctgaattccg cgggcccggg atcctctaga 661 gtcgacctgc agaagctatg gagctcctgc ggggactagg tgtcctgttc ctgttgcata 721 tgtgtggaag caaccgcatt ccagagtctg ggggagataa cggtgtgttt gacatctttg 781 aactcattgg aggtgcacga aggggccccg gtcgccgact ggtgaagggc caagatctat 841 ccagccccgc cttccgcatt gagaatgcca acctgatccc cgctgtgccg gatgacaagt 901 tccaagacct actggacgct gtgtgggccg acaaaggctt catcttcctg gcttccttga 961 ggcagatgaa gaagacccgg ggcacactcc tggctgtgga acggaaagac aacactggcc 1021 agatcttcag tgtggtctcc aacggcaaag ctggcaccct ggacctgagc ctgagcctgc 1081 cagggaagca acaagtggtg tcagtggagg aagctctcct ggccactggc cagtggaaga 1141 gcatcacgct gtttgttcaa gaggaccggg ctcaactcta catagactgt gataagatgg 1201 agagcgcgga gctggatgta cccatccaga gcatcttcac cagggatctg gccagcgttg 1261 ccaggctccg agttgcaaag ggagatgtca atgacaattt tcagggggtg ctgcagaatg 1321 tgaggtttgt ctttggaacc accccagaag acattctcag gaacaaaggc tgctccagct 1381 cagctaccaa cgtccttctt acccttgaca acaacgtggt gaacggttcc agccctgcta 1441 tccgcaccaa ctacatcggc cacaaaacaa aggacctcca agctatctgt ggcctctcct 1501 gtgatgaact atccagcatg gtcctggaac tgaagggcct gcgcaccatc gtgaccactc 1561 tgcaggacag catccgaaaa gtgacggaag agaacagaga gctggtcagt gagctgaagc 1621 ggcctcccct ctgctttcac aatggagtcc agtacaagaa caacgaggag tggactgtag 1681 acagttgcac agagtgtcac tgccagaact cggttaccat ctgcaaaaag gtgtcctgtc 1741 ccatcatgcc ctgctccaac gccacagttc ctgatggtga atgctgccca cggtgctggc 1801 ccagcgactc tgctgacgat ggctggtctc cctggtctga gtggacctcc tgctctgcca 1861 catgtggcaa tggaattcag caacgtggtc gttcctgtga cagcctcaac aacagatgcg 1921 agggctcttc ggtacagaca aggacctgcc acattcagga gtgtgacaaa agatttaaac 1981 aggatggtgg ctggagtcac tggtctccat ggtcgtcctg ttctgtgacc tgtggtgacg 2041 gtgtgatcac aaggatccgg ctctgcaact cccccagccc ccagatgaac gggaagccct 2101 gtgaaggtga agcccgggag accaaagcct gcaagaaaga cgcctgccca attaatggag 2161 gctggggtcc ctggtcacca tgggacatct gctctgtcac ctgtggagga ggagtgcaga 2221 gacgcagccg actctgtaac aaccccacac cccagtttgg aggcaaagac tgtgttggcg 2281 atgtgacaga aaatcaagtt tgcaacaagc aggactgccc aattgatgga tgcctgtcca 2341 atccctgctt tgctggtgcc aagtgtacta gctaccctga tggtagctgg aaatgtggtg 2401 cgtgtcctcc tggctacagt ggaaatggca tccagtgcaa agacgtcgat gagtgcaaag 2461 aagtgcctga tgcttgcttc aatcacaacg gagaacatcg gtgcaagaac acagatcctg 2521 gctacaactg cctgccctgc ccaccacgat tcactggctc acagcccttc ggccgaggtg 2581 tcgaacatgc catggccaac aaacaggtgt gcaaaccgcg aaacccctgc acggacggga 2641 cgcatgactg caacaagaac gctaagtgca actacctggg tcactacagt gaccccatgt 2701 accgctgtga gtgcaagccc ggctatgcag gcaatggcat catctgcgca gcttacccat 2761 acgatgttcc agattacgct tagctcgaga gatctaggcc gctaggcctc acctgcgatc 2821 tcgatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg 2881 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt 2941 gtgggaggtt ttttaaacta gcggccgcag gaacccctag tgatggagtt ggccactccc 3001 tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc 3061 tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg 3121 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat 3181 agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga 3241 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg 3301 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat 3361 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg 3421 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata 3481 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcgggctat tcttttgatt 3541 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat 3601 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa 3661 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc 3721 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga 3781 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg 3841 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg 3901 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 3961 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 4021 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc 4081 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg 4141 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc 4201 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat 4261 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg 4321 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag 4381 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa 4441 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc 4501 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca 4561 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc 4621 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc 4681 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg 4741 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta 4801 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag 4861 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga 4921 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc 4981 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 5041 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 5101 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 5161 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 5221 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 5281 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 5341 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 5401 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 5461 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 5521 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 5581 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 5641 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 5701 acatgt //