LOCUS       pENTR1A_huOGT__N-open        5353 bp    DNA     circular SYN 30-MAY-2020
DEFINITION  pENTR1A no ccDB (w48-1).
ACCESSION   urn.local...j-bzexmdp
KEYWORDS    .
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
            .
FEATURES             Location/Qualifiers
     source          1..5353
                     /organism="pENTR1A no ccDB (w48-1)"
                     /mol_type="other DNA"
     terminator      106..149
                     /label="rrnB_T1_terminator"
     terminator      281..308
                     /label="rrnB_T2_terminator"
     misc_feature    complement(358..457)
                     /label="attL1"
     misc_feature    complement(358..441)
                     /label="attL2"
     CDS             <460..3567
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /EC_number="2.4.1.255"
                     /note="isoform 1 is encoded by transcript variant 1;
                     O-GlcNAc transferase p110 subunit;
                     uridinediphospho-N-acetylglucosamine:polypeptide
                     beta-N-acetylglucosaminyl transferase;
                     UDP-N-acetylglucosamine--peptide
                     N-acetylglucosaminyltransferase 110 kDa subunit; O-GlcNAc
                     transferase subunit p110; O-linked N-acetylglucosamine
                     transferase 110 kDa subunit; O-linked N-acetylglucosamine
                     (GlcNAc) transferase
                     (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl
                     transferase)"
                     /codon_start=1
                     /product="UDP-N-acetylglucosamine--peptide
                     N-acetylglucosaminyltransferase 110 kDa subunit isoform 1"
                     /protein_id="NP_858058.1"
                     /db_xref="GI:32307148"
                     /db_xref="CCDS:CCDS14414.1"
                     /db_xref="GeneID:8473"
                     /db_xref="HGNC:HGNC:8127"
                     /db_xref="HPRD:02222"
                     /db_xref="MIM:300255"
                     /translation="MASSVGNVADSTEPTKRMLSFQGLAELAHREYQAGDFEAAERHC
                     MQLWRQEPDNTGVLLLLSSIHFQCRRLDRSAHFSTLAIKQNPLLAEAYSNLGNVYKER
                     GQLQEAIEHYRHALRLKPDFIDGYINLAAALVAAGDMEGAVQAYVSALQYNPDLYCVR
                     SDLGNLLKALGRLEEAKACYLKAIETQPNFAVAWSNLGCVFNAQGEIWLAIHHFEKAV
                     TLDPNFLDAYINLGNVLKEARIFDRAVAAYLRALSLSPNHAVVHGNLACVYYEQGLID
                     LAIDTYRRAIELQPHFPDAYCNLANALKEKGSVAEAEDCYNTALRLCPTHADSLNNLA
                     NIKREQGNIEEAVRLYRKALEVFPEFAAAHSNLASVLQQQGKLQEALMHYKEAIRISP
                     TFADAYSNMGNTLKEMQDVQGALQCYTRAIQINPAFADAHSNLASIHKDSGNIPEAIA
                     SYRTALKLKPDFPDAYCNLAHCLQIVCDWTDYDERMKKLVSIVADQLEKNRLPSVHPH
                     HSMLYPLSHGFRKAIAERHGNLCLDKINVLHKPPYEHPKDLKLSDGRLRVGYVSSDFG
                     NHPTSHLMQSIPGMHNPDKFEVFCYALSPDDGTNFRVKVMAEANHFIDLSQIPCNGKA
                     ADRIHQDGIHILVNMNGYTKGARNELFALRPAPIQAMWLGYPGTSGALFMDYIITDQE
                     TSPAEVAEQYSEKLAYMPHTFFIGDHANMFPHLKKKAVIDFKSNGHIYDNRIVLNGID
                     LKAFLDSLPDVKIVKMKCPDGGDNADSSNTALNMPVIPMNTIAEAVIEMINRGQIQIT
                     INGFSISNGLATTQINNKAATGEEVPRTIIVTTRSQYGLPEDAIVYCNFNQLYKIDPS
                     TLQMWANILKRVPNSVLWLLRFPAVGEPNIQQYAQNMGLPQNRIIFSPVAPKEEHVRR
                     GQLADVCLDTPLCNGHTTGMDVLWAGTPMVTMPGETLASRVAASQLTCLGCLELIAKN
                     RQEYEDIAVKLGTDLEYLKKVRGKVWKQRISSPLFNTKQYTMELERLYLQMWEHYAAG
                     NKPDHMIKPVEVTESA"
     gene            <460..>3567
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /note="O-linked N-acetylglucosamine (GlcNAc) transferase"
                     /db_xref="GeneID:8473"
                     /db_xref="HGNC:HGNC:8127"
                     /db_xref="HPRD:02222"
                     /db_xref="MIM:300255"
     source          <460..>3567
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq13"
     misc_feature    460..462
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="N-acetylalanine. {ECO:0000269|PubMed:19413330,
                     ECO:0000269|PubMed:22814378, ECO:0000269|Ref.5};
                     propagated from UniProtKB/Swiss-Prot (O15294.3);
                     acetylation site"
     exon            <460..493
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    460..3566
                     /Original_Bases="AAAGGAACCAATTCAGTCGACTGGATCCGGTACCGAATTCG
                     CGGCCGC"
                     /label="AAAGGAACCAATTCAGTCGACTGGATCCGGTACCGAATTCGCGGCCGC"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    463..465
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Phosphoserine, by GSK3-beta, alternate.
                     {ECO:0000250}; propagated from UniProtKB/Swiss-Prot
                     (O15294.3); phosphorylation site"
     misc_feature    466..468
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Phosphoserine, by GSK3-beta, alternate.
                     {ECO:0000250}; propagated from UniProtKB/Swiss-Prot
                     (O15294.3); phosphorylation site"
     misc_feature    <494..588
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 1"
     exon            <494..644
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     exon            645..888
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    691..792
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 2"
     misc_feature    793..894
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 3"
     exon            889..957
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    895..996
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 4"
     exon            958..1074
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    997..1098
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 5"
     exon            1075..1154
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    1099..1200
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 6"
     exon            1155..1350
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    1201..1302
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 7"
     motif           1226..1246
                     /created_by="User"
                     /label="z_p9"
     misc_feature    1303..1404
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 8"
     exon            1351..1491
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    1405..1506
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 9"
     exon            1492..1592
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    1507..1608
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 10"
     exon            1593..1746
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    1609..1710
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 11"
     misc_feature    1711..1812
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 12"
     exon            1747..1848
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    1813..1845
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 13, truncated"
     exon            1849..2028
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    1885..1935
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: Nuclear localization signal. {ECO:0000255}"
     exon            2029..2187
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     motif           2068..2087
                     /created_by="User"
                     /label="z_p10"
     exon            2188..2277
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     exon            2278..2403
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     exon            2404..2574
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     exon            2575..2691
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     STS             2633..3534
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /standard_name="Ogt"
                     /db_xref="UniSTS:507110"
     exon            2692..2862
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     exon            2863..3015
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     motif           2865..2884
                     /created_by="User"
                     /label="z_p11"
     exon            3016..3268
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     exon            3269..3392
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     exon            3393..>3567
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    3397..3456
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: Required for phosphatidylinositol
                     3,4,5-triphosphate binding"
     misc_feature    complement(3544..3567)
                     /created_by="primer3"
                     /%GC="41.7"
                     /Tm="59.0"
                     /Hairpin_Tm="33.4"
                     /Self_Dimer_Tm="None"
                     /Pair_Dimer_Tm="None"
                     /Sequence="TTATGCTGACTCAGTGACTTCAAC"
                     /Product_Size=3141
                     /annotation_group="14447722725616070001ad53f35-9fd9-41b0-9
                     a0f-4d59c2ea1588NM_181672 - OGT CDS"
                     /label="3,141 R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(3546..3567)
                     /created_by="primer3"
                     /%GC="40.9"
                     /Tm="57.1"
                     /Hairpin_Tm="33.4"
                     /Self_Dimer_Tm="1.4"
                     /Pair_Dimer_Tm="None"
                     /Sequence="TTATGCTGACTCAGTGACTTCA"
                     /Product_Size=3141
                     /annotation_group="14447722725604490000857aaf3-761a-4cb5-9
                     f4f-7e6b8107671bNM_181672 - OGT CDS"
                     /label="3,141 R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    3582..3681
                     /label="attL2"
     CDS             3804..4613
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGK
                     PDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVD
                     ASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRV
                     GIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
                     /label="ORF frame 1"
     gene            3804..4613
                     /gene="KanR2 (variant)"
                     /label="KanR2 (variant)"
     rep_origin      4687..5306
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGK
                     PDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVD
                     ASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRV
                     GIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
                     /label="pBR322_origin"
ORIGIN      
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggctttg cgtcttccgt gggcaacgtg
      481 gccgacagca cagggttagc tgagttggca catcgagaat atcaggcagg agattttgag
      541 gcagctgaga gacactgcat gcagctctgg agacaagagc cagacaatac tggtgtgctt
      601 ttattacttt catctataca cttccagtgt cgaaggctgg acagatctgc tcactttagc
      661 actctggcaa ttaaacagaa cccccttctg gcagaagctt attcgaattt ggggaatgtg
      721 tacaaggaaa gagggcagtt gcaggaggca attgagcatt atcgacatgc attgcgtctc
      781 aaacctgatt tcatcgatgg ttatattaac ctggcagccg ccttggtagc agcgggtgac
      841 atggaagggg cagtacaagc ttacgtctct gctcttcagt acaatcctga tttgtactgt
      901 gttcgcagtg acctggggaa cctgctcaaa gccctgggtc gcttggaaga agccaaggca
      961 tgttatttga aagcaattga gacgcaaccg aactttgcag tagcttggag taatcttggc
     1021 tgtgttttca atgcacaagg ggaaatttgg cttgcaattc atcactttga aaaggctgtc
     1081 acccttgacc caaactttct ggatgcttat atcaatttag gaaatgtctt gaaagaggca
     1141 cgcatttttg acagagctgt ggcagcttat cttcgtgccc taagtttgag tccaaatcac
     1201 gcagtggtgc acggcaacct ggcttgtgta tactatgagc aaggcctgat agatctggca
     1261 atagacacct acaggcgggc tatcgaacta caaccacatt tccctgatgc ttactgcaac
     1321 ctagccaatg ctctcaaaga gaagggcagt gttgctgaag cagaagattg ttataataca
     1381 gctctccgtc tgtgtcccac ccatgcagac tctctgaata acctagccaa tatcaaacga
     1441 gaacagggaa acattgaaga ggcagttcgc ttgtatcgta aagcattaga agtcttccca
     1501 gagtttgctg ctgcccattc aaatttagca agtgtactgc agcagcaggg aaaactgcag
     1561 gaagctctga tgcattataa ggaggctatt cgaatcagtc ctacctttgc tgatgcctac
     1621 tctaatatgg gaaacactct aaaggagatg caggatgttc agggagcctt gcagtgttat
     1681 acgcgtgcca tccaaattaa tcctgcattt gcagatgcac atagcaatct ggcttccatt
     1741 cataaggatt cagggaatat tccagaagcc atagcttctt accgcacggc tctgaaactt
     1801 aagcctgatt ttcctgatgc ttattgtaac ttggctcatt gcctgcagat tgtctgtgat
     1861 tggacagact atgatgagcg aatgaagaag ttggtcagta ttgtggctga ccagttagag
     1921 aagaataggt tgccttctgt gcatcctcat catagtatgc tatatcctct ttctcatggc
     1981 ttcaggaagg ctattgctga gaggcacggc aacctgtgct tagataagat taatgttctt
     2041 cataaaccac catatgaaca tccaaaagac ttgaagctca gtgatggtcg gctgcgtgta
     2101 ggatatgtga gttccgactt tgggaatcat cctacttctc accttatgca gtctattcca
     2161 ggcatgcaca atcctgataa atttgaggtg ttctgttatg ccctgagccc agacgatggc
     2221 acaaacttcc gagtgaaggt gatggcagaa gccaatcatt tcattgatct ttctcagatt
     2281 ccatgcaatg gaaaagcagc tgatcgcatc catcaggatg gaattcatat ccttgtaaat
     2341 atgaatggct atactaaggg cgctcgaaat gagctttttg ctctcaggcc agctcctatt
     2401 caggcaatgt ggctgggata ccctgggacg agtggtgcgc ttttcatgga ttatattatc
     2461 actgatcagg aaacttcgcc agctgaagtt gctgagcagt attccgagaa attggcttat
     2521 atgccccaca ctttttttat tggtgatcat gctaatatgt tccctcacct gaagaaaaaa
     2581 gcagtcatcg attttaagtc caatgggcac atttatgaca atcggatagt tctgaatggc
     2641 atcgacctca aagcatttct tgatagtcta ccagatgtga aaattgtcaa gatgaagtgt
     2701 cctgatggag gagacaatgc agatagcagt aacacagctc ttaatatgcc tgttattcct
     2761 atgaatacta ttgcagaagc agttattgaa atgattaacc gaggacagat tcaaataaca
     2821 attaatggat tcagtattag caatggactg gcaactactc agatcaacaa taaggctgca
     2881 actggagagg aggttccccg taccattatt gtaaccaccc gttctcagta cgggttacca
     2941 gaagatgcca tcgtatactg taactttaat cagttgtata aaattgaccc ttctactttg
     3001 cagatgtggg caaacattct gaagcgtgtt cccaatagtg tactctggct gttgcgtttt
     3061 ccagcagtag gagaacctaa tattcaacag tatgcacaaa acatgggcct gccccagaac
     3121 cgtatcattt tttcacctgt tgctcctaaa gaggaacacg tcaggagagg ccagctggct
     3181 gatgtctgct tggacactcc actctgtaat gggcacacca cagggatgga tgtcctctgg
     3241 gcagggaccc ccatggtgac tatgccagga gagactcttg cttctcgagt tgcagcatcc
     3301 cagctcactt gcttaggttg tcttgagctt attgctaaaa acagacaaga atatgaagac
     3361 atagctgtga agctgggaac tgatctagaa tacctgaaga aagttcgtgg caaagtctgg
     3421 aagcaaagaa tatctagccc tctgttcaac accaaacaat acacaatgga actagagcgg
     3481 ctctatctac agatgtggga gcattatgca gctggcaaca aacctgacca catgattaag
     3541 cctgttgaag tcactgagtc agcataactc gagatatcta gacccagctt tcttgtacaa
     3601 agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag
     3661 tcaaaataaa atcattattt gccatccagc tgcagctctg gcccgtgtct caaaatctct
     3721 gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca
     3781 taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat
     3841 taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc
     3901 aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga
     3961 aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc
     4021 tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat
     4081 ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg
     4141 attcaggtga aaatattgtt gatgcgctgg cagtgtccct gcgccggttg cattcgattc
     4201 ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac
     4261 gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg
     4321 ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca
     4381 ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta
     4441 ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact
     4501 gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata
     4561 atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat
     4621 tggttaattg gttgtaacat tattcagatt gggccccgtt ccactgagcg tcagacccgg
     4681 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     4741 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     4801 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     4861 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     4921 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     4981 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     5041 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     5101 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     5161 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     5221 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     5281 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     5341 ttgctcacat gtt
//
