LOCUS       16_C1-MPAct-mRuby3      4968 bp ds-DNA     circular     28-MAR-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      4190..4872
                     /label="ColE1\origin"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             2867..3658
                     /label="Kan/neoR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1585..1633
                     /label="Kras04-CAAX-tail"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        2633..2829
                     /label="SV40\early\promoter"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             698..1558
                     /label="Ftractin-mRuby3"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     rep_origin      complement(2016..2322)
                     /label="F1\ori"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     promoter        131..657
                     /label="CMV\promotor"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(1599..1658)
                     /label="p131-C1-CaaX-Not1-Rev"
                     /note="sequence: attatgatctagagtcgcggccgctCTACATAATTACACACTTTGTCTTTGACTTCTTTT"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          668..714
                     /label="p136-HindIII-C1-FT-for"
                     /note="sequence: ctaccggactcagatctcgagctcaagcttatgggcatggcgcgacc"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          668..722
                     /label="p168-C1-HindIII-FT-for-long"
                     /note="sequence: ctaccggactcagatctcgagctcaagcttatgggcatggcgcgaccacggggcg"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          677..711
                     /label="C1-gibson-cAct-HIndIII-35bp-F"
                     /note="sequence: tcagatctcgagctcaagcttatgggcatggcgcg"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          689..714
                     /label="C1-HindIII-cAct-For"
                     /note="sequence: ctcaagcttatgggcatggcgcgacc"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(1637..1662)
                     /label="p058-NotI-XbaI-Rev"
                     /note="sequence: gctgattatgatctagagtcgcggcc"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          complement(1623..1657)
                     /label="C1-cActin-Not1-35bp-rev"
                     /note="sequence: ttatgatctagagtcgcggccgctCTACATAATTA"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(1701..1718)
                     /label="FastBacRev"
                     /note="sequence: AGGGGGAGGTGTGGGAGG"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
ORIGIN
        1 ccaccaatac ccgactctat atatcataga cgacgaacga gctaacgaac gacaaacact
       61 aataccaccg tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      121 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      181 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      241 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      301 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      361 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      421 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      481 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      541 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      601 gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc
      661 gctagcgcta ccggactcag atctcgagct caagcttatg ggcatggcgc gaccacgggg
      721 cgcggggccc tgcagccccg ggttggagcg ggctccgcgc cggagcgtcg gggagctgcg
      781 cctgctcttc gaagcgcgct gcgccgcagt cgccgccgca gcggccgcag ggtcggatcc
      841 accggtcatg gtgtctaagg gcgaagagct gatcaaggaa aatatgcgta tgaaggtggt
      901 catggaaggt tcggtcaacg gccaccaatt caaatgcaca ggtgaaggag aaggcagacc
      961 gtacgaggga gtgcaaacca tgaggatcaa agtcatcgag ggaggacccc tgccatttgc
     1021 ctttgacatt cttgccacgt cgttcatgta tggcagccgt acctttatca agtacccggc
     1081 cgacatccct gatttcttta aacagtcctt tactgagggt tttacttggg aaagagttac
     1141 gagatacgaa gatggtggag tcgtcaccgt cacgcaggac accagccttg aggatggcga
     1201 gctcgtctac aacgtcaagg tcagaggggt aaactttccc tccaatggtc ccgtgatgca
     1261 gaagaagacc aagggttggg agcctaatac agagatgatg tatccagcag atggtggtct
     1321 gagaggatac actgacatcg cactgaaagt tgatggtggt ggccatctgc actgcaactt
     1381 cgtgacaact tacaggtcaa aaaagaccgt cgggaacatc aagatgcccg gtgtccatgc
     1441 cgttgatcac cgcctggaaa ggatcgagga gagtgacaat gaaacctacg tagtgcaaag
     1501 agaagtggca gttgccaaat acagcaacct tggtggtggc atggacgagc tgtacaagAG
     1561 ATCTCGAGCT CAAGCTTCGA ATTCTGGTAA AAAGAAGAAA AAGAAGTCAA AGACAAAGTG
     1621 TGTAATTATG TAGagcggcc gcgactctag atcataatca gccataccac atttgtagag
     1681 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     1741 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     1801 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     1861 ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt
     1921 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     1981 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     2041 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     2101 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     2161 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     2221 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     2281 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     2341 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     2401 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     2461 aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga
     2521 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     2581 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     2641 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     2701 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     2761 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     2821 ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     2881 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     2941 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     3001 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg
     3061 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     3121 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     3181 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     3241 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     3301 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     3361 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt
     3421 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     3481 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     3541 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     3601 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     3661 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     3721 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     3781 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg
     3841 ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa
     3901 agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag
     3961 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct
     4021 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc
     4081 ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa
     4141 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     4201 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     4261 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     4321 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     4381 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     4441 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     4501 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     4561 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     4621 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     4681 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     4741 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     4801 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     4861 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     4921 cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcat
//