LOCUS       22_C1-mCit-FKBP-EZRabd( 5317 bp ds-DNA     circular     28-MAR-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    3260..3577
                     /label="FKBP"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    2432..3259
                     /label="g-block-mCitrine-opt"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    3755..3757
                     /label="T567D"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     promoter        1895..2421
                     /label="CMV\promotor"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     rep_origin      complement(4199..4505)
                     /label="F1\ori"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    3230..3850
                     /label="gBlock-FKBP-FT-EZRABD(t567D)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      1056..1738
                     /label="ColE1\origin"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        4816..5012
                     /label="SV40\early\promoter"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             join(5050..5317,1..524)
                     /label="Kan/neoR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3172..3178
                     /label="eCFP"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          3168..3217
                     /label="p189-ecoRI-EEVEE-for"
                     /note="sequence: agctGTACAAGAGATCTCGAGCTCAAGCTTCGaccggtggtggatccgga"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          complement(3884..3901)
                     /label="FastBacRev"
                     /note="sequence: AGGGGGAGGTGTGGGAGG"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(3164..3259)
                     /label="p195_mCit-SAGG-Rev"
                     /note="sequence: TCCCCCCGCACTTCCACCAGCGCTCCCGCCagcactggttcctccggatccaccaccggtCGAAGCTTGAGCTCGAGATCTCTTGTACagctcgtc"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          2422..2480
                     /label="p194-C1-Mcit-for"
                     /note="sequence: tccgctagcgctaccggactcagatctcgagctcaagcttatggtgagcaagggcgagg"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
ORIGIN
        1 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
       61 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
      121 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
      181 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
      241 tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
      301 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
      361 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
      421 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
      481 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
      541 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
      601 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
      661 gcgcggggat ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg
      721 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac
      781 gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt
      841 cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc
      901 ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc
      961 tgccatagcc tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt
     1021 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     1081 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     1141 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     1201 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     1261 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     1321 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     1381 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     1441 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     1501 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     1561 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     1621 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     1681 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     1741 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     1801 tgattctgtg gataaccgta ttaccgccat gcattagtta ttaatagtaa tcaattacgg
     1861 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     1921 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     1981 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     2041 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     2101 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     2161 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     2221 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     2281 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     2341 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
     2401 ctggtttagt gaaccgtcag atccgctagc gctaccggac tcagatctcg agctcaagct
     2461 tatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     2521 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     2581 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     2641 cctcgtgacc accttcggct acggcctgat gtgcttcgcc cgctaccccg accacatgaa
     2701 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     2761 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     2821 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     2881 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     2941 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     3001 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     3061 ctacctgagc taccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt
     3121 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tGTACAAGAG
     3181 ATCTCGAGCT CAAGCTTCGa ccggtggtgg atccggagga accagtgctG GCGGGAGCGC
     3241 TGGTGGAAGT GCGGGGGGAG TCCAGGTGGA AACTATAAGT CCGGGTGACG GCCGGACCTT
     3301 TCCAAAACGG GGGCAGACTT GTGTTGTTCA CTATACTGGG ATGTTGGAAG ATGGGAAAAA
     3361 ATTCGATTCC AGTCGAGACC GGAATAAACC GTTCAAATTC ATGTTGGGTA AACAGGAAGT
     3421 AATTCGAGGA TGGGAAGAGG GAGTGGCTCA GATGTCTGTA GGCCAACGAG CTAAACTTAC
     3481 GATCTCCCCA GATTACGCCT ACGGAGCTAC TGGCCACCCG GGTATTATCC CCCCTCACGC
     3541 GACGCTCGTC TTTGACGTTG AACTCCTTAA ACTCGAATCT GGTGGGACAA GCGCGGGTGG
     3601 AAGCGCTGGA GGTTCTGCTG GGGGCTCAGC TGGTGGATCA GCTGGAGGTA GCGGCTCCGC
     3661 AGGGGGTTCT GCAGGAGGCA GCACCAGCGC TGGCGGATCA GCAGGTGGCa tgatccacaa
     3721 cgagaacatg aggcaaggcc gggacaagta caagGATctg cggcagatcc ggcagggcaa
     3781 caccaagcag cgcatcgacg agttcgaggc cctgTAAgcg gccgcgactc tagatcataa
     3841 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     3901 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     3961 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     4021 attctagttg tggtttgtcc aaactcatca atgtatctta aggcgtaaat tgtaagcgtt
     4081 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     4141 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     4201 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     4261 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     4321 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     4381 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     4441 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     4501 aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     4561 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     4621 taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga
     4681 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     4741 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     4801 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     4861 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     4921 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     4981 gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg
     5041 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     5101 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     5161 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     5221 gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     5281 gctgtgctcg acgttgtcac tgaagcggga agggact
//