LOCUS       pSLQ5428_pHR_EF1       13551 bp ds-DNA     circular     19-JUN-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pHR-TRE3G-NLS-dCas9-eGFP-NLS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 21, 2013 from SnapGene 2.0.1 http://www.snapgene.com
COMMENT     from pSLQ1645
COMMENT     Added cut sites between dCas9-NLS and NLS-eGFP for future cloning
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     polyA_signal    243..377
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
                     /locus_tag="SV40 poly(A) signal"
                     /label="SV40 poly(A) signal"
                     /ApEinfo_label="SV40 poly(A) signal"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     LTR             546..1179
                     /note="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /locus_tag="3' LTR"
                     /label="3' LTR"
                     /ApEinfo_label="3' LTR"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1226..1351
                     /note="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /locus_tag="HIV-1 Psi"
                     /label="HIV-1 Psi"
                     /ApEinfo_label="HIV-1 Psi"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1848..2081
                     /note="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /locus_tag="RRE"
                     /label="RRE"
                     /ApEinfo_label="RRE"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    7765..7785
                     /locus_tag="sv40 NLS"
                     /label="sv40 NLS"
                     /ApEinfo_label="sv40 NLS"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    7828..7893
                     /locus_tag="3x Flag Tag"
                     /label="3x Flag Tag"
                     /ApEinfo_label="3x Flag Tag"
                     /ApEinfo_fwdcolor="#800080"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(8430..8441)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /translation="IEGR"
                     /locus_tag="Factor Xa recognition and cleavage site"
                     /label="Factor Xa recognition and cleavage site"
                     /ApEinfo_label="Factor Xa recognition and cleavage site"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_signal    complement(182..373)
                     /locus_tag="SV40 late polyA"
                     /label="SV40 late polyA"
                     /ApEinfo_label="SV40 late polyA"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    999..1179
                     /locus_tag="HIV-1_5_LTR"
                     /label="HIV-1_5_LTR"
                     /ApEinfo_label="HIV-1_5_LTR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    246..377
                     /locus_tag="SV40_PA_terminator"
                     /label="SV40_PA_terminator"
                     /ApEinfo_label="SV40_PA_terminator"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    7949..8549
                     /locus_tag="wPRE"
                     /label="wPRE"
                     /ApEinfo_label="wPRE"
                     /ApEinfo_fwdcolor="#0060ff"
                     /ApEinfo_revcolor="#0060ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    334..353
                     /locus_tag="EBV_rev_primer"
                     /label="EBV_rev_primer"
                     /ApEinfo_label="EBV_rev_primer"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     LTR             8657..8890
                     /note="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR)
                     from HIV-1"
                     /locus_tag="3' LTR (Delta-U3)"
                     /label="3' LTR (Delta-U3)"
                     /ApEinfo_label="3' LTR (Delta-U3)"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        complement(8915..8933)
                     /note="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /locus_tag="SP6 promoter"
                     /label="SP6 promoter"
                     /ApEinfo_label="SP6 promoter"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      10841..11460
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /locus_tag="pBR322 ori"
                     /label="pBR322 ori"
                     /ApEinfo_label="pBR322 ori"
                     /ApEinfo_fwdcolor="#c0c0c0"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        11691..12020
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
                     /locus_tag="SV40 promoter"
                     /label="SV40 promoter"
                     /ApEinfo_label="SV40 promoter"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4855..7755
                     /locus_tag="Rfx Cas13d"
                     /label="Rfx Cas13d"
                     /ApEinfo_label="Rfx Cas13d"
                     /ApEinfo_fwdcolor="#8080c0"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     intron          13155..13219
                     /note="small t intron"
                     /note="simian virus 40 (SV40) small t antigen intron"
                     /locus_tag="small t intron"
                     /label="small t intron"
                     /ApEinfo_label="small t intron"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5569..5586
                     /locus_tag="HEPN1"
                     /label="HEPN1"
                     /ApEinfo_label="HEPN1"
                     /ApEinfo_fwdcolor="#cccce6"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     3976..3996
                     /note="Human elongation factor-1a promoter, forward
                     primer"
                     /locus_tag="EF1a-F"
                     /label="EF1a-F"
                     /ApEinfo_label="EF1a-F"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             13349..13369
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T
                     antigen"
                     /note="SV40 NLS"
                     /note=" "
                     /protein_id=" "
                     /translation="PKKKRKV"
                     /locus_tag="nuclear localization signal of SV40 large T
                     antig"
                     /label="nuclear localization signal of SV40 large T
                     antig"
                     /ApEinfo_label="nuclear localization signal of SV40 large
                     T  antig"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             9826..10686
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="#ffff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    7795..7821
                     /locus_tag="c-Myc NLS"
                     /label="c-Myc NLS"
                     /ApEinfo_label="c-Myc NLS"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    9756..9784
                     /locus_tag="AmpR promoter"
                     /label="AmpR promoter"
                     /ApEinfo_label="AmpR promoter"
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="#ffff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    7426..7443
                     /locus_tag="HEPN2"
                     /label="HEPN2"
                     /ApEinfo_label="HEPN2"
                     /ApEinfo_fwdcolor="#c9c9e4"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    8710..8890
                     /locus_tag="HIV-1_5_LTR(1)"
                     /label="HIV-1_5_LTR(1)"
                     /ApEinfo_label="HIV-1_5_LTR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    11692..11907
                     /locus_tag="SV40_enhancer"
                     /label="SV40_enhancer"
                     /ApEinfo_label="SV40_enhancer"
                     /ApEinfo_fwdcolor="#92ff59"
                     /ApEinfo_revcolor="#92ff59"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    13153..13168
                     /locus_tag="SV40_int"
                     /label="SV40_int"
                     /ApEinfo_label="SV40_int"
                     /ApEinfo_fwdcolor="#409bff"
                     /ApEinfo_revcolor="#409bff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    13174..13221
                     /locus_tag="SV40_3_splice"
                     /label="SV40_3_splice"
                     /ApEinfo_label="SV40_3_splice"
                     /ApEinfo_fwdcolor="#3a82ff"
                     /ApEinfo_revcolor="#3a82ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    8640..8654
                     /locus_tag="cPPT"
                     /label="cPPT"
                     /ApEinfo_label="cPPT"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    11704..11775
                     /locus_tag="SV40 early promoter 72-repeat region"
                     /label="SV40 early promoter 72-repeat region"
                     /ApEinfo_label="SV40 early promoter 72-repeat region"
                     /ApEinfo_fwdcolor="#ff9f38"
                     /ApEinfo_revcolor="#ff9f38"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    11776..11847
                     /locus_tag="SV40 early promoter 72-repeat region(1)"
                     /label="SV40 early promoter 72-repeat region(1)"
                     /ApEinfo_label="SV40 early promoter 72-repeat region"
                     /ApEinfo_fwdcolor="#ff9f38"
                     /ApEinfo_revcolor="#ff9f38"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4068..4077
                     /locus_tag="Kozak"
                     /label="Kozak"
                     /ApEinfo_label="Kozak"
                     /ApEinfo_fwdcolor="#800080"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    11851..11871
                     /locus_tag="SV40 early promoter 21-repeat region"
                     /label="SV40 early promoter 21-repeat region"
                     /ApEinfo_label="SV40 early promoter 21-repeat region"
                     /ApEinfo_fwdcolor="#59ff9a"
                     /ApEinfo_revcolor="#59ff9a"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    11872..11892
                     /locus_tag="SV40 early promoter 21-repeat region(1)"
                     /label="SV40 early promoter 21-repeat region(1)"
                     /ApEinfo_label="SV40 early promoter 21-repeat region"
                     /ApEinfo_fwdcolor="#59ff9a"
                     /ApEinfo_revcolor="#59ff9a"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    11894..11914
                     /locus_tag="SV40 early promoter 21-repeat region (3)"
                     /label="SV40 early promoter 21-repeat region (3)"
                     /ApEinfo_label="SV40 early promoter 21-repeat region (3)"
                     /ApEinfo_fwdcolor="#53ff97"
                     /ApEinfo_revcolor="#53ff97"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2612..2626
                     /locus_tag="cPPT(1)"
                     /label="cPPT(1)"
                     /ApEinfo_label="cPPT"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        11824..12020
                     /locus_tag="SV40 early promoter"
                     /label="SV40 early promoter"
                     /ApEinfo_label="SV40 early promoter"
                     /ApEinfo_fwdcolor="cornflower blue"
                     /ApEinfo_revcolor="cornflower blue"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    7786..7794
                     /locus_tag="linker"
                     /label="linker"
                     /ApEinfo_label="linker"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     intron          3081..4019
                     /note="intron upstream of the start codon of human
                     EF-1-alpha"
                     /locus_tag="EF-1-alpha intron A"
                     /label="EF-1-alpha intron A"
                     /ApEinfo_label="EF-1-alpha intron A"
                     /ApEinfo_fwdcolor="#eb606c"
                     /ApEinfo_revcolor="#eb606c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2582..2732
                     /locus_tag="cPPT(2)"
                     /label="cPPT(2)"
                     /ApEinfo_label="cPPT"
                     /ApEinfo_fwdcolor="#ff85f7"
                     /ApEinfo_revcolor="#ff85f7"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        2850..4028
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
                     /locus_tag="EF-1-alpha promoter"
                     /label="EF-1-alpha promoter"
                     /ApEinfo_label="EF-1-alpha promoter"
                     /ApEinfo_fwdcolor="#ffffb5"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4078..4785
                     /note="mCherry"
                     /locus_tag="mCherry"
                     /label="mCherry"
                     /ApEinfo_label="mCherry"
                     /ApEinfo_fwdcolor="#c40000"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4795..4851
                     /note="P2A"
                     /locus_tag="P2A"
                     /label="P2A"
                     /ApEinfo_label="P2A"
                     /ApEinfo_fwdcolor="#80ffff"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    7757..7761
                     /locus_tag="SpeI"
                     /label="SpeI"
                     /ApEinfo_label="SpeI"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4849..4854
                     /locus_tag="XmaI"
                     /label="XmaI"
                     /ApEinfo_label="XmaI"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    7756..7756
                     /locus_tag="SpeI(1)"
                     /label="SpeI(1)"
                     /ApEinfo_label="SpeI"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    7822..7827
                     /locus_tag="BamHI"
                     /label="BamHI"
                     /ApEinfo_label="BamHI"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    7897..7902
                     /locus_tag="BsiWI"
                     /label="BsiWI"
                     /ApEinfo_label="BsiWI"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4063..4070
                     /locus_tag="AscI"
                     /label="AscI"
                     /ApEinfo_label="AscI"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    7762..7764
                     /locus_tag="linker(1)"
                     /label="linker(1)"
                     /ApEinfo_label="linker"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 catactgttt tttcttactc cacacaggca tagagtgtct gctattaata actatgctca
       61 aaaattgtgt acctttagct ttttaatttg taaaggggtt aataaggaat atttgatgta
      121 tagtgccttg actagagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
      181 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
      241 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
      301 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
      361 cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca
      421 ccccataccc tattaccact gccaattacc tagtggtttc atttactcta aacctgtgat
      481 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta
      541 gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc tgtggatcta
      601 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata
      661 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga
      721 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga
      781 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc
      841 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact
      901 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct
      961 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga
     1021 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     1081 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     1141 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     1201 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     1261 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     1321 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     1381 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     1441 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     1501 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     1561 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     1621 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     1681 acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     1741 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     1801 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     1861 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     1921 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     1981 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     2041 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     2101 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     2161 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     2221 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     2281 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     2341 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     2401 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     2461 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     2521 agagacagag acagatccat tcgattagtg aacggatcgg cactgcgtgc gccaattctg
     2581 cagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
     2641 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     2701 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg
     2761 gttaattaat gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga
     2821 ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca
     2881 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     2941 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     3001 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     3061 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta
     3121 cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac gtgattcttg
     3181 atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc
     3241 cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct
     3301 ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt
     3361 gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc
     3421 tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc
     3481 gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt
     3541 ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct
     3601 gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg
     3661 gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt
     3721 cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg
     3781 agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt
     3841 taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg
     3901 aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg
     3961 gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg
     4021 tgtcgtgaac gcgtctgcag gatacaagct tgcggtaccg cgggcgcgcc cgccaccatg
     4081 gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg
     4141 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
     4201 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc
     4261 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc
     4321 gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
     4381 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc
     4441 gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg
     4501 cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc
     4561 ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag
     4621 gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac
     4681 atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc
     4741 gccgagggcc gccactccac cggcggcatg gacgagctgt acaagggcag cggagctact
     4801 aacttcagcc tgctgaagca ggctggagac gtggaggaga accctggacc cgggatgatc
     4861 gaGaaaaaGa agtccttcgc Taagggcatg ggcgtgaagt ccacactcgt gtccggctcc
     4921 aaagtgtaca tgacaacctt cgccgaaggc agcgacgcca ggctggaaaa gatcgtggag
     4981 ggcgacagca tcaggagcgt gaatgagggc gaggccttca gcgctgaaat ggccgataaa
     5041 aacgccggct ataagatcgg caacgccaaa ttcagccatc ctaagggcta cgccgtggtg
     5101 gctaacaacc ctctgtatac aggacccgtc cagcaggata tgctcggcct gaaggaaact
     5161 ctggaaaaga ggtacttcgg cgagagcgct gatggcaatg acaatatttg tatccaggtg
     5221 atccataaca tcctggacat tgaaaaaatc ctcgccgaat acattaccaa cgccgcctac
     5281 gccgtcaaca atatctccgg cctggataag gacattattg gattcggcaa gttctccaca
     5341 gtgtatacct acgacgaatt caaagacccc gagcaccata gggccgcttt caacaataac
     5401 gataagctca tcaacgccat caaggcccag tatgacgagt tcgacaactt cctcgataac
     5461 cccagactcg gctatttcgg ccaggccttt ttcagcaagg agggcagaaa ttacatcatc
     5521 aattacggca acgaatgcta tgacattctg gccctcctga gcggactgAG Acactgggtg
     5581 gtcCACaaca acgaagaaga gtccaggatc tccaggacct ggctctacaa cctcgataag
     5641 aacctcgaca acgaatacat ctccaccctc aactacctct acgacaggat caccaatgag
     5701 ctgaccaact ccttctccaa gaactccgcc gccaacgtga actatattgc cgaaactctg
     5761 ggaatcaacc ctgccgaatt cgccgaacaa tatttcagat tcagcattat gaaagagcag
     5821 aaaaacctcg gattcaatat caccaagctc agggaagtga tgctggacag gaaggatatg
     5881 tccgagatca ggaaaaatca taaggtgttc gactccatca ggaccaaggt ctacaccatg
     5941 atggactttg tgatttatag gtattacatc gaagaggatg ccaaggtggc tgccgccaat
     6001 aagtccctcc ccgataatga gaagtccctg agcgagaagg atatctttgt gattaacctg
     6061 aggggctcct tcaacgacga ccagaaggat gccctctact acgatgaagc taatagaatt
     6121 tggagaaagc tcgaaaatat catgcacaac atcaaggaat ttaggggaaa caagacaaga
     6181 gagtataaga agaaggacgc ccctagactg cccagaatcc tgcccgctgg ccgtgatgtt
     6241 tccgccttca gcaaactcat gtatgccctg acAatgttcc tggatggcaa ggagatcaac
     6301 gacctcctga ccaccctgat taataaattc gataacatcc agagcttcct gaaggtgatg
     6361 cctctcatcg gagtcaacgc taagttcgtg gaggaatacg cctttttcaa agactccgcc
     6421 aagatcgccg atgagctgag gctgatcaag tccttcgcta gaatgggaga acctattgcc
     6481 gatgccagga gggccatgta tatcgacgcc atccgtattt taggaaccaa cctgtcctat
     6541 gatgagctca aggccctcgc cgacaccttt tccctggacg agaacggaaa caagctcaag
     6601 aaaggcaagc acggcatgag aaatttcatt attaataacg tgatcagcaa taaaaggttc
     6661 cactacctga tcagatacgg tgatcctgcc cacctccatg agatcgccaa aaacgaggcc
     6721 gtggtgaagt tcgtgctcgg caggatcgct gacatccaga aaaaacaggg ccagaacggc
     6781 aagaaccaga tcgacaggta ctacgaaact tgtatcggaa aggataaggg caagagcgtg
     6841 agcgaaaagg tggacgctct cacaaagatc atcaccggaa tgaactacga ccaattcgac
     6901 aagaaaagga gcgtcattga ggacaccggc agggaaaacg ccgagaggga gaagtttaaa
     6961 aagatcatca gcctgtacct caccgtgatc taccacatcc tcaagaatat tgtcaatatc
     7021 aacgccaggt acgtcatcgg attccattgc gtcgagcgtg atgctcaact gtacaaggag
     7081 aaaggctacg acatcaatct caagaaactg gaagagaagg gattcagctc cgtcaccaag
     7141 ctctgcgctg gcattgatga aactgccccc gataagagaa aggacgtgga aaaggagatg
     7201 gctgaaagag ccaaggagag cattgacagc ctcgagagcg ccaaccccaa gctgtatgcc
     7261 aattacatca aatacagcga cgagaagaaa gccgaggagt tcaccaggca gattaacagg
     7321 gagaaggcca aaaccgccct gaacgcctac ctgaggaaca ccaagtggaa tgtgatcatc
     7381 agggaggacc tcctgagaat tgacaacaag acatgtaccc tgttcAGGaa caaggccgtc
     7441 CATctggaag tggccaggta tgtccacgcc tatatcaacg acattgccga ggtcaattcc
     7501 tacttccaac tgtaccatta catcatgcag agaattatca tgaatgagag gtacgagaaa
     7561 agcagcggaa aggtgtccga gtacttcgac gctgtgaatg acgagaagaa gtacaacgat
     7621 aggctcctga aactgctgtg tgtgcctttc ggctactgta tccccaggtt taagaacctg
     7681 agcatcgagg ccctgttcga taggaacgag gccgccaagt tcgacaagga gaaaaagaaA
     7741 gtgtccggca attccACTAG Tgctcccaag aaaaagcgca aggtaggtgg aagcccagca
     7801 gctaaaagag ttaaattgga tggatccGAT TATAAGGATC ACGATGGAGA TTACAAGGAC
     7861 CACGACATAG ACTACAAAGA TGATGACGAC AAGtaaCGTA cggccgcgac tctagagtcg
     7921 acctgcaggc atgcaagctt gatatcaagc ttatcgataa tcaacctctg gattacaaaa
     7981 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     8041 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     8101 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     8161 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     8221 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     8281 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     8341 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     8401 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     8461 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     8521 ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgaggg aattaattcg
     8581 agctcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     8641 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt
     8701 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     8761 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     8821 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     8881 atctctagca gcatctagaa ttaattccgt gtattctata gtgtcaccta aatcgtatgt
     8941 gtatgataca taaggttatg tattaattgt agccgcgttc taacgacaat atgtacaagc
     9001 ctaattgtgt agcatctggc ttactgaagc agaccctatc atctctctcg taaactgccg
     9061 tcagagtcgg tttggttgga cgaaccttct gagtttctgg taacgccgtc ccgcacccgg
     9121 aaatggtcag cgaaccaatc agcagggtca tcgctagcca gatcctctac gccggacgca
     9181 tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca
     9241 ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta
     9301 tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc
     9361 ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt
     9421 cgcataaggg agagcgtcga atggtgcact ctcagtacaa tctgctctga tgccgcatag
     9481 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     9541 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     9601 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     9661 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     9721 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     9781 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     9841 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     9901 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     9961 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
    10021 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
    10081 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
    10141 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
    10201 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
    10261 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
    10321 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
    10381 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
    10441 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
    10501 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
    10561 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
    10621 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
    10681 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
    10741 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
    10801 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
    10861 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
    10921 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
    10981 agagcgcaga taccaaatac tgtctttcta gtgtagccgt agttaggcca ccacttcaag
    11041 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
    11101 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
    11161 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
    11221 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
    11281 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
    11341 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
    11401 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
    11461 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
    11521 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
    11581 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
    11641 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctgtggaat gtgtgtcagt
    11701 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
    11761 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
    11821 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
    11881 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
    11941 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
    12001 gaggcctagg cttttgcaaa aagcttggac acaagacagg cttgcgagat atgtttgaga
    12061 ataccacttt atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa
    12121 atactggttt ttagtgcgcc agatctctat aatctcgcgc aacctatttt cccctcgaac
    12181 actttttaag ccgtagataa acaggctggg acacttcaca tgagcgaaaa atacatcgtc
    12241 acctgggaca tgttgcagat ccatgcacgt aaactcgcaa gccgactgat gccttctgaa
    12301 caatggaaag gcattattgc cgtaagccgt ggcggtctgt accgggtgcg ttactggcgc
    12361 gtgaactggg tattcgtcat gtcgataccg tttgtatttc cagctacgat cacgacaacc
    12421 agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg
    12481 atgacctggt ggataccggt ggtactgcgg ttgcgattcg tgaaatgtat ccaaaagcgc
    12541 actttgtcac catcttcgca aaaccggctg gtcgtccgct ggttgatgac tatgttgttg
    12601 atatcccgca agatacctgg attgaacagc cgtgggatat gggcgtcgta ttcgtcccgc
    12661 caatctccgg tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt ctttttaact
    12721 tcaggcgggt tacaatagtt tccagtaagt attctggagg ctgcatccat gacacaggca
    12781 aacctgagcg aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc gacgctagtc
    12841 cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg cccttgatgg taaaaccatc
    12901 cctcactggt atcgcatgat taaccgtctg atgtggatct ggcgcggcat tgacccacgc
    12961 gaaatcctcg acgtccaggc acgtattgtg atgagcgatg ccgaacgtac cgacgatgat
    13021 ttatacgata cggtgattgg ctaccgtggc ggcaactgga tttatgagtg ggccccggat
    13081 ctttgtgaag gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat
    13141 ttaaagctct aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct
    13201 aattgtttgt gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa
    13261 tgcctttaat gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc
    13321 tactgctgac tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa
    13381 ggactttcct tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct
    13441 tgcttgcttt gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat
    13501 ggaaaaatat tctgtaacct ttataagtag gcataacagt tataatcata a
//
