LOCUS       pMZ828                  6613 bp    DNA     circular     24-JUN-2020
DEFINITION  PCaMV35S-PhyB(1-650)-VP16-NLS-Tnos.
ACCESSION   urn.local...4m-bxnh8ab
KEYWORDS    .
SOURCE      null
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     primer_bind     665..685
                     /created_by="primer3"
                     /annotation_group="368344346793449b2fd01e7-579d-418a-a8e8-
                     98348d47a09d"
                     /%GC="52.4"
                     /Tm="58.2"
                     /Hairpin_Tm="53.1"
                     /Self_Dimer_Tm="None"
                     /Sequence="CTATCCTTCGCAAGACCCTTC"
                     /Transferred_From="35S promoter "
                     /Transferred_Similarity="100.00%"
                     /label="35S promoter "
     ORF             791..3187
                     /Genetic_code="Standard"
                     /Reading_frame=1
                     /created_by="ORF Finder"
                     /modified_by="User"
                     /label="PhyB-VP16-NLS"
     CDS             791..2740
                     /label="PhyB(1-650)"
     misc_feature    1669
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    2750..2776
                     /label="Linker"
     CDS             2777..3163
                     /modified_by="User"
                     /label="VP16 gene"
     sig_peptide     3164..3184
                     /label="NLS"
     terminator      3290..3542
                     /note="nopaline synthase terminator and poly(A) signal"
                     /modified_by="User"
                     /label="Tnos"
     primer_bind     complement(3594..3614)
                     /created_by="primer3"
                     /annotation_group="368498081522160f56bf3d8-15a9-4f55-8207-
                     100fb1c7acab"
                     /%GC="42.9"
                     /Tm="54.5"
                     /Hairpin_Tm="36.1"
                     /Self_Dimer_Tm="None"
                     /Sequence="GCAATTAACCCTCACTAAAGG"
                     /Transferred_From="T3 "
                     /Transferred_Similarity="100.00%"
                     /label="T3 "
     primer_bind     complement(3646..3668)
                     /created_by="primer3"
                     /annotation_group="3681122420212641c0712f0-c42c-4e1e-a842-
                     656678a279c5"
                     /%GC="43.5"
                     /Tm="59.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="AGCGGATAACAATTTCACACAGG"
                     /Transferred_From="M13/pUC Reverse"
                     /Transferred_Similarity="100.00%"
                     /label="M13/pUC Reverse"
     rep_origin      complement(4035..4623)
                     /direction="LEFT; RIGHT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /modified_by="User"
                     /label="ori"
     primer_bind     complement(4115..4134)
                     /created_by="primer3"
                     /annotation_group="368013014609700e515e0e9-9633-4f68-b574-
                     2e898e0c518f"
                     /%GC="50.0"
                     /Tm="57.9"
                     /Hairpin_Tm="36.7"
                     /Self_Dimer_Tm="None"
                     /Sequence="GGGAAACGCCTGGTATCTTT"
                     /Transferred_From="pBR322ori-F"
                     /Transferred_Similarity="100.00%"
                     /label="pBR322ori-F"
     CDS             order(complement(5586..5654),complement(4794..5585))
                     /modified_by="User"
                     /label="AmpR"
     primer_bind     5417..5436
                     /created_by="primer3"
                     /annotation_group="368315469795102f7820b24-c956-49ef-8962-
                     5734406f9c9f"
                     /%GC="50.0"
                     /Tm="58.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="ATAATACCGCGCCACATAGC"
                     /Transferred_From="Amp-R "
                     /Transferred_Similarity="100.00%"
                     /label="Amp-R "
     promoter        complement(5655..5759)
                     /label="AmpR promoter"
     rep_origin      complement(5785..6240)
                     /direction="LEFT"
                     /note="arrow  indicates direction of (+) strand synthesis;
                     f1 bacteriophage origin of replication"
                     /modified_by="User"
                     /label="f1 ori"
     primer_bind     complement(5922..5943)
                     /created_by="primer3"
                     /annotation_group="3684274549334999fa52d5c-da05-437b-9cd0-
                     d103c403e83b"
                     /%GC="50.0"
                     /Tm="59.4"
                     /Hairpin_Tm="39.7"
                     /Self_Dimer_Tm="None"
                     /Sequence="GTGGACTCTTGTTCCAAACTGG"
                     /Transferred_From="F1ori-F "
                     /Transferred_Similarity="100.00%"
                     /label="F1ori-F "
     primer_bind     6367..6389
                     /created_by="primer3"
                     /annotation_group="368095086814295ab653c1c-dfb1-49a7-a63e-
                     08de6aa4b3c5"
                     /%GC="52.2"
                     /Tm="62.4"
                     /Hairpin_Tm="50.0"
                     /Self_Dimer_Tm="16.6"
                     /Sequence="CCCAGTCACGACGTTGTAAAACG"
                     /Transferred_From="M13/pUC Forward"
                     /Transferred_Similarity="100.00%"
                     /label="M13/pUC Forward"
     primer_bind     6405..6424
                     /created_by="primer3"
                     /annotation_group="368513478869144dcf9d8a1-f8f6-4d2c-8401-
                     e956510671a8"
                     /%GC="40.0"
                     /Tm="50.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TAATACGACTCACTATAGGG"
                     /Transferred_From="T7"
                     /Transferred_Similarity="100.00%"
                     /label="T7"
     promoter        6471..731
                     /modified_by="User"
                     /label="PCaMV35S "
ORIGIN      
        1 aataccttcc caagaaggtt aaagatgcag tcaaaagatt caggactaac tgcatcaaga
       61 acacagagaa agatatattt ctcaagatca gaagtactat tccagtatgg acgattcaag
      121 gcttgcttca caaaccaagg caagtaatag agattggagt ctctaaaaag gtagttccca
      181 ctgaatcaaa ggccatggag tcaaagattc aaatagagga cctaacagaa ctcgccgtaa
      241 agactggcga acagttcata cagagtctct tacgactcaa tgacaagaag aaaatcttcg
      301 tcaacatggt ggagcacgac acacttgtct actccaaaaa tatcaaagat acagtctcag
      361 aagaccaaag ggcaattgag acttttcaac aaagggtaat atccggaaac ctcctcggat
      421 tccattgccc agctatctgt cactttattg tgaagatagt ggaaaaggaa ggtggctcct
      481 acaaatgcca tcattgcgat aaaggaaagg ccatcgttga agatgcctct gccgacagtg
      541 gtcccaaaga tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca
      601 cgtcttcaaa gcaagtggat tgatgtgata tcaccactga cgtaagggat gacgcacaat
      661 cccactatcc ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagaa
      721 cacgggggac tctagcgcta ccggttggct aggtaagctt ggtaccacct gaacgacgca
      781 tatgacaaca atggtttccg gagtcggggg tagtggcggt ggccgtggcg gtggccgtgg
      841 cggagaagaa gaaccgtcgt caagtcacac tcctaataac cgaagaggag gagaacaagc
      901 tcaatcgtcg ggaacgaaat ctctcagacc aagaagcaac actgaatcaa tgagcaaagc
      961 aattcaacag tacaccgtcg acgcaagact ccacgccgtt ttcgaacaat ccggcgaatc
     1021 agggaaatca ttcgactact cacaatcact caaaacgacg acgtacggtt cctctgtacc
     1081 tgagcaacag atcacagctt atctctctcg aatccagcga ggtggttaca ttcagccttt
     1141 cggatgtatg atcgccgtcg atgaatccag tttccggatc atcggttaca gtgaaaacgc
     1201 cagagaaatg ttagggatta tgcctcaatc tgttcctact cttgagaaac ctgagattct
     1261 agctatggga actgatgtga gatctttgtt cacttcttcg agctcgattc tactcgagcg
     1321 tgctttcgtt gctcgagaga ttaccttgtt aaatccggtt tggatccatt ccaagaatac
     1381 tggtaaaccg ttttacgcca ttcttcatag gattgatgtt ggtgttgtta ttgatttaga
     1441 gccagctaga actgaagatc ctgcgctttc tattgctggt gctgttcaat cgcagaaact
     1501 cgcggttcgt gcgatttctc agttacaggc tcttcctggt ggagatatta agcttttgtg
     1561 tgacactgtc gtggaaagtg tgagggactt gactggttat gatcgtgtta tggtttataa
     1621 gtttcatgaa gatgagcatg gagaagttgt agctgagagt aaacgagacg atttagagcc
     1681 ttatattgga ctgcattatc ctgctactga tattcctcaa gcgtcaaggt tcttgtttaa
     1741 gcagaaccgt gtccgaatga tagtagattg caatgccaca cctgttcttg tggtccagga
     1801 cgataggcta actcagtcta tgtgcttggt tggttctact cttagggctc ctcatggttg
     1861 tcactctcag tatatggcta acatgggatc tattgcgtct ttagcaatgg cggttataat
     1921 caatggaaat gaagatgatg ggagcaatgt agctagtgga agaagctcga tgaggctttg
     1981 gggtttggtt gtttgccatc acacttcttc tcgctgcata ccgtttccgc taaggtatgc
     2041 ttgtgagttt ttgatgcagg ctttcggttt acagttaaac atggaattgc agttagcttt
     2101 gcaaatgtca gagaaacgcg ttttgagaac gcagacactg ttatgtgata tgcttctgcg
     2161 tgactcgcct gctggaattg ttacacagag tcccagtatc atggacttag tgaaatgtga
     2221 cggtgcagca tttctttacc acgggaagta ttacccgttg ggtgttgctc ctagtgaagt
     2281 tcagataaaa gatgttgtgg agtggttgct tgcgaatcat gcggattcaa ccggattaag
     2341 cactgatagt ttaggcgatg cggggtatcc cggtgcagct gcgttagggg atgctgtgtg
     2401 cggtatggca gttgcatata tcacaaaaag agactttctt ttttggtttc gatctcacac
     2461 tgcgaaagaa atcaaatggg gaggcgctaa gcatcatccg gaggataaag atgatgggca
     2521 acgaatgcat cctcgttcgt cctttcaggc ttttcttgaa gttgttaaga gccggagtca
     2581 gccatgggaa actgcggaaa tggatgcgat tcactcgctc cagcttattc tgagagactc
     2641 ttttaaagaa tctgaggcgg ctatgaactc taaagttgtg gatggtgtgg ttcagccatg
     2701 tagggatatg gcgggggaac aggggattga tgagttaggt gaattcgata gtgctggtag
     2761 tgctggtagt gctggttccg cgtacagccg cgcgcgtacg aaaaacaatt acgggtctac
     2821 catcgagggc ctgctcgatc tcccggacga cgacgccccc gaagaggcgg ggctggcggc
     2881 tccgcgcctg tcctttctcc ccgcgggaca cacgcgcaga ctgtcgacgg cccccccgac
     2941 cgatgtcagc ctgggggacg agctccactt agacggcgag gacgtggcga tggcgcatgc
     3001 cgacgcgcta gacgatttcg atctggacat gttgggggac ggggattccc cgggtccggg
     3061 atttaccccc cacgactccg ccccctacgg cgctctggat atggccgact tcgagtttga
     3121 gcagatgttt accgatgccc ttggaattga cgagtacggt gggccaaaga agaagaggaa
     3181 ggtgtaggca acggctagca cgacgtgatg cggcagcggc cgaattcccc gggctcgaga
     3241 agcttggatc caccggatct agataactga tctcgaggcg aatttccccg atcgttcaaa
     3301 catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat
     3361 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt
     3421 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa
     3481 caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga
     3541 tcgggaatta attcgataag ctagagatcc gtcaaggtac ccagcttttg ttccctttag
     3601 tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     3661 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     3721 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     3781 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     3841 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     3901 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     3961 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     4021 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     4081 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     4141 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     4201 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     4261 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     4321 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     4381 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     4441 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     4501 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     4561 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     4621 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     4681 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     4741 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     4801 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     4861 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     4921 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     4981 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     5041 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     5101 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     5161 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     5221 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     5281 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     5341 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     5401 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     5461 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     5521 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     5581 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     5641 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     5701 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     5761 acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta aaattcgcgt
     5821 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     5881 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     5941 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     6001 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     6061 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     6121 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     6181 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     6241 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     6301 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     6361 ggttttccca gtcacgacgt tgtaaaacga cggccagtga attgtaatac gactcactat
     6421 agggcgaatt ggagctccac cgcggagcgg agaattaagg gagtcaggcc ttaattaaga
     6481 gctcgcatgc ctgcaggtcc ccagattagc cttttcaatt tcagaaagaa tgctaaccca
     6541 cagatggtta gagaggctta cgcagcaggt ctcatcaaga cgatctaccc gagcaataat
     6601 ctccaggaaa tca
//
