LOCUS       pNBA001                11442 bp    DNA     circular UNA 24-JUN-2020
DEFINITION  (etr)8-(C120)5-PhCMVmin-EFR-GFP-T35S.
ACCESSION   urn.local...3jd-bxstnxb
KEYWORDS    .
SOURCE      null
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     cds             371..1162
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /Transferred_Translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVH
                     LYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTI
                     VVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGP
                     AAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKD
                     VAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
                     /Transferred_From="Xpre2-S"
                     /Transferred_Similarity="100.00%"
                     /label="SmR"
     region          1540..1562
                     /label="Left Border"
     repeat_region   1620..1906
                     /label="(etr)8 "
     repeat_region   1922..2041
                     /label="(C120)5 "
     promoter        2064..2163
                     /label="PhCMVmin"
     gene            2178..5357
                     /feature_type="Insertion"
                     /origin="EFR genomic (3,207bp linear)Fragment: from BsaI
                     cut at 9^10 to BsaI cut at 3194^3195, 3,185bpOrientation:
                     originalPlus strand 5': no changePlus strand 3': no
                     changeMinus strand 5': no changeMinus strand 3': no
                     change"
                     /modified_by="User"
                     /label="gEFR"
     intron          4879..4965
                     /modified_by="User"
                     /label="Intron"
     misc_feature    5364..5408
                     /modified_by="User"
                     /label="15 aa linker"
     CDS             5409..6125
                     /gene="sGFP"
                     /note="sGFP (S65T) gene"
                     /codon_start=1
                     /transl_table=11
                     /product="sGFP"
                     /protein_id="BAG12994.1"
                     /db_xref="GI:170172452"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITHGMDELYK"
                     /label="sGFP (ATG removed)"
     terminator      6130..6355
                     /modified_by="User"
                     /label="T35S"
     region          complement(6416..6440)
                     /label="Right Border"
     modified_base   6470
                     /modified_by="User"
                     /label="T addition"
     CDS             6756..7442
                     /modified_by="User"
                     /label="pVS1 resolvase"
     rep_origin      7549..10334
                     /label="pVS1 ori (short)"
     cds             7741..8370
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /Transferred_Translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGAD
                     VLLVDSDPQGSARDWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADL
                     AVSAIKAADFVLIPVQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRI
                     GGEVAEALAGYELPILESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKS
                     KLI"
                     /Transferred_From="Xpre2-S, pCambia-addgene"
                     /Transferred_Similarity="100.00%"
                     /label="pVS1 StaA"
     modified_base   8750
                     /modified_by="User"
                     /label="T>C"
     cds             8799..9872
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid
                      pVS1 (Heeb et al., 2000)"
                     /Transferred_Translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQR
                     QRIEAEARPGESWQAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTR
                     DSRSIMDVAVFRLSKRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHL
                     TESMNRYREGKGDKPGRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQR
                     TKKAKNGRLVTVSEGEALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTV
                     HPDYFLIDPGIGRFLYRLARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGS
                     NDLPEYDLKEEAGQAGPILVMRYRNLIEGEASAGS"
                     /Transferred_From="Xpre2-S"
                     /Transferred_Similarity="100.00%"
                     /label="pVS1 RepA"
     modified_base   8803
                     /modified_by="User"
                     /label="G addtion"
     modified_base   9332
                     /modified_by="User"
                     /label="C>A"
     rep_origin      9938..10132
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /Transferred_From="Xpre2-S"
                     /Transferred_Similarity="100.00%"
                     /label="pVS1 oriV"
     misc_feature    10476..10616
                     /note="basis of mobility region from pBR322"
                     /Transferred_From="Xpre2-S"
                     /Transferred_Similarity="100.00%"
                     /label="bom"
     rep_origin      complement(10802..11390)
                     /direction="LEFT; RIGHT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /label="ori"
     primer_bind     complement(10882..10901)
                     /note="pBR322 origin, forward primer"
                     /X-Transferred_From="Xpre2-S"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="BM00654 (reversed)"
                     /Transferred_Similarity="100.00%"
                     /label="pBR322ori-F"
     modified_base   11319
                     /modified_by="User"
                     /label="C>A"
ORIGIN      
        1 actaagctgc cgggtttgaa acacggatga tctcgcggag ggtagcatgt tgattgtaac
       61 gatgacagag cgttgctgcc tgtgatcaat tcgggcacga acccagtgga cataagcctg
      121 ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt
      181 gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt
      241 ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg
      301 atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa
      361 gttaaacatc atgggggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt
      421 tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc
      481 agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag
      541 gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc
      601 tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat
      661 tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat
      721 tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa
      781 agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt
      841 tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc
      901 cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc
      961 agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc
     1021 ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga
     1081 tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat
     1141 caccaaggta gtcggcaaat aatgtctagc tagaaattcg ttcaagccga cgccgcttcg
     1201 cggcgcggct taactcaagc gttagatgca ctaagcacat aattgctcac agccaaacta
     1261 tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccctac acaaattggg
     1321 agatatatca tgcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     1381 ccccgtagaa aagatcaaag gatcttctgc cggtaacatg agcaaagtct gccgccttac
     1441 aacggctctc ccgctgacgc cgtcccggac tgatgggctg cctgtatcga gtggtgattt
     1501 tgtgccgagc tgccggtcgg ggagctgttg gctggctggt ggcaggatat attgtggtgt
     1561 aaacaaattg acgcttagac ggattacgga agacaatacg gcggcttcga agtttaaacg
     1621 attgaatata accgacgtga ctgttacatt tagggattga atataaccga cgtgactgtt
     1681 acatttaggg attgaatata accgacgtga ctgttacatt tagggattga atataaccga
     1741 cgtgactgtt acatttaggg ataaacgatt gaatataacc gacgtgactg ttacatttag
     1801 ggattgaata taaccgacgt gactgttaca tttagggatt gaatataacc gacgtgactg
     1861 ttacatttag ggattgaata taaccgacgt gactgttaca tttagggatg ctagcctcga
     1921 gtaggtagcc tttagtccat gcgttatagg tagcctttag tccatgcgtt ataggtagcc
     1981 tttagtccat gcgttatagg tagcctttag tccatgcgtt ataggtagcc tttagtccat
     2041 gaagcttaga cactagagct agccctatat aagcagagct cgtttagtga accgtcagat
     2101 cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag
     2161 ccttctctgg aagcaccatg aagctgtcct tttcacttgt tttcaatgct ctcacgttgc
     2221 ttcttcaagt ttgcatcttt gctcaagcca ggttttctaa tgagactgat atgcaagctt
     2281 tgcttgagtt caagtctcaa gtttctgaaa acaacaagag agaggtcttg gcttcatgga
     2341 atcactcctc cccattttgt aattggattg gagtcacatg tggccgcagg agagaaagag
     2401 ttataagttt gaaccttgga ggattcaagt taaccggtgt gatctcaccc tccattggta
     2461 atctctcctt tcttagatta cttaatcttg cagacaactc ttttggaagt accatccctc
     2521 aaaaggtggg aaggctattt aggcttcagt acttgaacat gagctataat cttctcgaag
     2581 gaaggattcc gtctagtctt tctaactgct ctagactgtc gaccgttgat ttatcgtcaa
     2641 accatcttgg acatggtgtt ccttcagaac taggttcact ttctaagctt gccattctgg
     2701 atcttagcaa aaacaacctt actggaaatt ttcctgcatc tttaggaaac ttgacgtcac
     2761 ttcagaagct tgactttgca tataaccaga tgagaggtga gattccagac gaagtagcta
     2821 gattgactca aatggtgttt ttccaaatag cactgaatag tttttcaggt ggttttcctc
     2881 ctgcattgta caacatctcc tctcttgagt ctctatctct agctgacaat agcttttcgg
     2941 gtaatcttag ggctgatttt ggttatcttc taccaaatct aagaagactt cttttgggaa
     3001 caaatcagtt cactggagct attcccaaaa cacttgccaa tatctcaagc cttgaaaggt
     3061 ttgatatctc atctaattac ctgtctggta gtatcccttt gagctttgga aagttacgta
     3121 atctgtggtg gttagggatt cgtaataact ctcttggaaa taactcgtcc agtggtcttg
     3181 aatttattgg ggctgtggcg aactgcactc aattagagta cttagatgtt ggttacaata
     3241 gacttggagg tgagcttcct gcttctatag ccaatctgtc cactacattg actagtctgt
     3301 tccttggaca aaatcttatc tctggaacca ttcctcatga catcgggaat cttgtaagcc
     3361 tgcaagaact cagcttagaa acaaatatgt tgagtggaga acttcccgtc tctttcggga
     3421 agcttttgaa cttgcaggtt gtggatctgt attcaaatgc aatatcgggg gaaataccat
     3481 cttattttgg caacatgact cggttgcaga agctccattt gaatagcaat agtttccacg
     3541 gaagaatccc tcagagtctt ggacgttgtc gatacttgct agacctgtgg atggatacaa
     3601 ataggttgaa tgggactata cctcaggaaa tactgcaaat tccatccctc gcttacatag
     3661 atttgtcaaa caatttcttg acaggccatt ttccagaaga agttggaaag ttagaacttc
     3721 ttgttggact aggtgcttcg tacaacaaat tatcaggaaa gatgccacaa gctatagggg
     3781 gttgtctttc gatggaattt ctctttatgc aaggaaattc gtttgacgga gccattccag
     3841 atataagccg gttggtaagc ctaaagaatg ttgacttctc caacaacaat ctctctggcc
     3901 ggatacctcg atatctggcc agtcttcctt cgctgcgaaa tctgaatctt tctatgaaca
     3961 agtttgaggg aagggtgcca acaacaggag tgtttcgaaa tgctacagca gtttctgttt
     4021 ttggtaacac aaatatttgc ggaggcgtcc gagaaatgca actaaagcca tgcattgtac
     4081 aggcatctcc aaggaagaga aagcctctgt cagttagaaa gaaagttgtc agtggtattt
     4141 gtataggtat agcttcgctt ttgttaatca taattgtggc ttctctgtgt tggttcatga
     4201 agaggaaaaa gaaaaacaat gccagtgatg gtaacccatc tgattctact actttgggga
     4261 tgttccatga gaaggtaagt tatgaagagc ttcatagtgc aacaagtcgc ttctcttcaa
     4321 ccaatttgat tggttcaggc aatttcggta atgtgtttaa aggattgctt ggccctgaga
     4381 ataaactcgt cgcggttaaa gttttgaacc tcctaaagca tggagcgacg aaaagcttta
     4441 tggcggaatg tgaaaccttc aagggtatac gacatcgtaa ccttgtaaaa ctgataacgg
     4501 tttgttcaag ccttgattcc gagggaaatg atttcagagc tctggtctat gagttcatgc
     4561 caaaaggaag tctggatatg tggctgcagc tagaagatct ggaaagggta aacgatcact
     4621 cgagatcttt aacacccgca gagaaactca acatagcaat agatgtggct tcagctttgg
     4681 agtatctgca cgttcattgt catgaccctg tagctcactg tgatattaag ccaagcaaca
     4741 ttcttctaga cgatgatctg actgctcatg ttagtgactt tggtttggct cagctcctct
     4801 ataaatacga tcgagaatcc tttctaaacc agtttagttc tgctggtgtc agaggcacca
     4861 ttggctatgc cgcgccaggt taataaacat ctatatgcaa aaagtattgt ttaacaaaag
     4921 agttatccaa cctcattgtg tgttgtttct ttgaaatatg tgcagagtat ggaatgggag
     4981 gccaaccatc aatacaagga gatgtgtaca gcttcggaat tctacttttg gagatgttta
     5041 gtggaaagaa accaacagat gaatcatttg caggcgatta taacctccac agctacacaa
     5101 agtctatatt atcgggttgc acgagcagtg gaggcagcaa cgccattgat gaggggttga
     5161 gactggtttt gcaggtgggg ataaagtgtt ctgaagaata tccgagggat aggatgagaa
     5221 cggatgaagc agtacgagaa ttaatctcaa taagatctaa gttcttcagt tccaagacga
     5281 ctattacaga gagtcctcga gatgctccgc aaagttctcc tcaggaatgg atgttaaata
     5341 cggacatgca tactatgaag ggaggtggag gaggttctgg aggcggtgga agtggtggcg
     5401 gaggtagcgt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     5461 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     5521 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     5581 ccaccctcgt gaccaccttc acctacggcg tgcagtgctt cagccgctac cccgaccaca
     5641 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     5701 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     5761 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     5821 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     5881 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     5941 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     6001 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     6061 tggtcctgct ggagttcgtg accgccgccg ggatcactca cggcatggac gagctgtaca
     6121 agtaaaatcc ggccatgcta gagtccgcaa aaatcaccag tctctctcta caaatctatc
     6181 tctctctatt tttctccaga ataatgtgtg agtagttccc agataaggga attagggttc
     6241 ttatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta tttgtatttg
     6301 taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt gaccttgagt
     6361 gacctaagct agcctagtgt cgggcttgtc ttctctggtt taaactatca gtgtttgaca
     6421 ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataat cggatattta
     6481 aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa ccacagggtt
     6541 cccctcggga tcaaagtact ttgatccaac ccctccgctg ctatagtgca gtcggcttct
     6601 gacgttcagt gcagccttct tctgaaaacg acatgtcgca caagtcctaa gttacgcgac
     6661 aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt
     6721 agaatacttg cgactagaac cggagacatt acgccatgaa caagagcgcc gccgctggcc
     6781 tgctgggcta tgcccgcgtc agcaccgacg accaggactt gaccaaccaa cgggccgaac
     6841 tgcacgcggc cggctgcacc aagctgtttt ccgagaagat caccggcacc aggcgcgacc
     6901 gcccggagct ggccaggatg cttgaccacc tacgccctgg cgacgttgtg acagtgacca
     6961 ggctagaccg cctggcccgc agcacccgcg acctactgga cattgccgag cgcatccagg
     7021 aggccggcgc gggcctgcgt agcctggcag agccgtgggc cgacaccacc acgccggccg
     7081 gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg
     7141 accgcacccg gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt ggcccccgcc
     7201 ctaccctcac cccggcacag atcgcgcacg cccgcgagct gatcgaccag gaaggccgca
     7261 ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg
     7321 agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg
     7381 cattgaccga ggccgacgcc ctggcggccg ccgagaatga acgccaagag gaacaagcat
     7441 gaaaccgcac caggacggcc aggacgaacc gtttttcatt accgaagaga tcgaggcgga
     7501 gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac atctcaaccg tgcggctgca
     7561 tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc tggccggcca gcttggccgc
     7621 tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg
     7681 tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa caaatacgca aggggaacgc
     7741 atgaaggtta tcgctgtact taaccagaaa ggcgggtcag gcaagacgac catcgcaacc
     7801 catctagccc gcgccctgca actcgccggg gccgatgttc tgttagtcga ttccgatccc
     7861 cagggcagtg cccgcgattg ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc
     7921 atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg
     7981 atcgacggag cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc agccgacttc
     8041 gtgctgattc cggtgcagcc aagcccttac gacatatggg ccaccgccga cctggtggag
     8101 ctggttaagc agcgcattga ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg
     8161 cgggcgatca aaggcacgcg catcggcggt gaggttgccg aggcgctggc cgggtacgag
     8221 ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct acccaggcac tgccgccgcc
     8281 ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg
     8341 gccgctgaaa ttaaatcaaa actcatttga gttaatgagg taaagagaaa atgagcaaaa
     8401 gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg
     8461 ccagcctggc agacacgcca gccatgaagc gggtcaactt tcagttgccg gcggaggatc
     8521 acaccaagct gaagatgtac gcggtacgcc aaggcaagac cattaccgag ctgctatctg
     8581 aatacatcgc gcagctacca gagtaaatga gcaaatgaat aaatgagtag atgaatttta
     8641 gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc
     8701 cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg gctgggttgc ctgccggccc
     8761 tgcaatggca ctggaacccc caagcccgag gaatcggcgt gagcggtcgc aaaccatccg
     8821 gcccggtaca aatcggcgcg gcgctgggtg atgacctggt ggagaagttg aaggccgcgc
     8881 aggccgccca gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg
     8941 ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc agccggtgcg ccgtcgatta
     9001 ggaagccgcc caagggcgac gagcaaccag attttttcgt tccgatgctc tatgacgtgg
     9061 gcacccgcga tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc
     9121 gacgagctgg cgaggtgatc cgctacgagc ttccagacgg gcacgtagag gtttccgcag
     9181 ggccggccgg catggccagt gtgtgggatt acgacctggt actgatggcg gtttcccatc
     9241 taaccgaatc catgaaccga taccgggaag ggaagggaga caagcccggc cgcgtgttcc
     9301 gtccacacgt tgcggacgta ctcaagttct gccggcgagc cgatggcgga aagcagaaag
     9361 acgacctggt agaaacctgc attcggttaa acaccacgca cgttgccatg cagcgtacga
     9421 agaaggccaa gaacggccgc ctggtgacgg tatccgaggg tgaagccttg attagccgct
     9481 acaagatcgt aaagagcgaa accgggcggc cggagtacat cgagatcgag ctagctgatt
     9541 ggatgtaccg cgagatcaca gaaggcaaga acccggacgt gctgacggtt caccccgatt
     9601 actttttgat cgatcccggc atcggccgtt ttctctaccg cctggcacgc cgcgccgcag
     9661 gcaaggcaga agccagatgg ttgttcaaga cgatctacga acgcagtggc agcgccggag
     9721 agttcaagaa gttctgtttc accgtgcgca agctgatcgg gtcaaatgac ctgccggagt
     9781 acgatttgaa ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc taccgcaacc
     9841 tgatcgaggg cgaagcatcc gccggttcct aatgtacgga gcagatgcta gggcaaattg
     9901 ccctagcagg ggaaaaaggt cgaaaaggac tctttcctgt ggatagcacg tacattggga
     9961 acccaaagcc gtacattggg aaccggaacc cgtacattgg gaacccaaag ccgtacattg
    10021 ggaaccggtc acacatgtaa gtgactgata taaaagagaa aaaaggcgat ttttccgcct
    10081 aaaactcttt aaaacttatt aaaactctta aaacccgcct ggcctgtgca taactgtctg
    10141 gccagcgcac agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac
    10201 gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac
    10261 ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc
    10321 cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
    10381 cagctcccgg agacagtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
    10441 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc
    10501 gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
    10561 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct
    10621 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
    10681 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
    10741 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
    10801 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
    10861 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
    10921 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
    10981 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
    11041 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
    11101 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
    11161 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
    11221 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
    11281 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
    11341 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
    11401 tgatcttttc tacggggtct gacgctcatc ggaagaacgg ca
//
