LOCUS       pROF018                 5327 bp ds-DNA     circular SYN 24-JUN-2020
DEFINITION  PCaMV35S-NLS-KRAB-EL222-Tnos.
ACCESSION
VERSION     .
KEYWORDS    pROF018.
SOURCE      null
  ORGANISM  
            .
REFERENCE   1  (bases 1 to 5455)
  AUTHORS   Trial and User
  TITLE     Direct Submission
  JOURNAL   Exported Montag, 27. Apr 2015 from SnapGene Viewer 2.7.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     primer_bind     665..685
                     /created_by="primer3"
                     /annotation_group="368344346793449b2fd01e7-579d-418a-a8e8-
                     98348d47a09d"
                     /%GC="52.4"
                     /Tm="58.2"
                     /Hairpin_Tm="53.1"
                     /Self_Dimer_Tm="None"
                     /Sequence="CTATCCTTCGCAAGACCCTTC"
                     /Transferred_From="35S promoter "
                     /Transferred_Similarity="100.00%"
                     /label="35S promoter "
     ORF             803..1855
                     /Genetic_code="Standard"
                     /Reading_frame=2
                     /created_by="ORF Finder"
                     /modified_by="User"
                     /label="2xNLS-KRAB-EL222"
     sig_peptide     806..829
                     /created_by="User"
                     /label="NLS"
     sig_peptide     830..853
                     /created_by="User"
                     /label="NLS"
     CDS             854..1220
                     /modified_by="User"
                     /label="KRAB"
     CDS             1226..1852
                     /modified_by="User"
                     /label="EL222"
     terminator      2004..2256
                     /modified_by="User"
                     /label="Tnos"
     primer_bind     complement(2308..2328)
                     /created_by="primer3"
                     /annotation_group="368498081522160f56bf3d8-15a9-4f55-8207-
                     100fb1c7acab"
                     /%GC="42.9"
                     /Tm="54.5"
                     /Hairpin_Tm="36.1"
                     /Self_Dimer_Tm="None"
                     /Sequence="GCAATTAACCCTCACTAAAGG"
                     /Transferred_From="T3 "
                     /Transferred_Similarity="100.00%"
                     /label="T3 "
     primer_bind     complement(2360..2382)
                     /created_by="primer3"
                     /annotation_group="3681122420212641c0712f0-c42c-4e1e-a842-
                     656678a279c5"
                     /%GC="43.5"
                     /Tm="59.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="AGCGGATAACAATTTCACACAGG"
                     /Transferred_From="M13/pUC Reverse"
                     /Transferred_Similarity="100.00%"
                     /label="M13/pUC Reverse"
     rep_origin      complement(2749..3337)
                     /Transferred_Similarity="100.00% -> 99.32%"
                     /label="ori"
     primer_bind     complement(2829..2848)
                     /created_by="primer3"
                     /annotation_group="368013014609700e515e0e9-9633-4f68-b574-
                     2e898e0c518f"
                     /%GC="50.0"
                     /Tm="57.9"
                     /Hairpin_Tm="36.7"
                     /Self_Dimer_Tm="None"
                     /Sequence="GGGAAACGCCTGGTATCTTT"
                     /Transferred_From="pBR322ori-F"
                     /Transferred_Similarity="100.00%"
                     /label="pBR322ori-F"
     CDS             order(complement(4300..4368),complement(3508..4299))
                     /label="AmpR"
     primer_bind     4131..4150
                     /created_by="primer3"
                     /annotation_group="368315469795102f7820b24-c956-49ef-8962-
                     5734406f9c9f"
                     /%GC="50.0"
                     /Tm="58.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="ATAATACCGCGCCACATAGC"
                     /Transferred_From="Amp-R "
                     /Transferred_Similarity="100.00%"
                     /label="Amp-R "
     promoter        complement(4369..4473)
                     /modified_by="User"
                     /label="AmpR promoter"
     rep_origin      complement(4499..4954)
                     /modified_by="User"
                     /label="f1 ori"
     primer_bind     complement(4636..4657)
                     /created_by="primer3"
                     /annotation_group="3684274549334999fa52d5c-da05-437b-9cd0-
                     d103c403e83b"
                     /%GC="50.0"
                     /Tm="59.4"
                     /Hairpin_Tm="39.7"
                     /Self_Dimer_Tm="None"
                     /Sequence="GTGGACTCTTGTTCCAAACTGG"
                     /Transferred_From="F1ori-F "
                     /Transferred_Similarity="100.00%"
                     /label="F1ori-F "
     primer_bind     5081..5103
                     /created_by="primer3"
                     /annotation_group="368095086814295ab653c1c-dfb1-49a7-a63e-
                     08de6aa4b3c5"
                     /%GC="52.2"
                     /Tm="62.4"
                     /Hairpin_Tm="50.0"
                     /Self_Dimer_Tm="16.6"
                     /Sequence="CCCAGTCACGACGTTGTAAAACG"
                     /Transferred_From="M13/pUC Forward"
                     /Transferred_Similarity="100.00%"
                     /label="M13/pUC Forward"
     primer_bind     5119..5138
                     /created_by="primer3"
                     /annotation_group="368513478869144dcf9d8a1-f8f6-4d2c-8401-
                     e956510671a8"
                     /%GC="40.0"
                     /Tm="50.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TAATACGACTCACTATAGGG"
                     /Transferred_From="T7"
                     /Transferred_Similarity="100.00%"
                     /label="T7"
     promoter        5185..730
                     /modified_by="User"
                     /label="PCaMV35S "
ORIGIN      
        1 aataccttcc caagaaggtt aaagatgcag tcaaaagatt caggactaac tgcatcaaga
       61 acacagagaa agatatattt ctcaagatca gaagtactat tccagtatgg acgattcaag
      121 gcttgcttca caaaccaagg caagtaatag agattggagt ctctaaaaag gtagttccca
      181 ctgaatcaaa ggccatggag tcaaagattc aaatagagga cctaacagaa ctcgccgtaa
      241 agactggcga acagttcata cagagtctct tacgactcaa tgacaagaag aaaatcttcg
      301 tcaacatggt ggagcacgac acacttgtct actccaaaaa tatcaaagat acagtctcag
      361 aagaccaaag ggcaattgag acttttcaac aaagggtaat atccggaaac ctcctcggat
      421 tccattgccc agctatctgt cactttattg tgaagatagt ggaaaaggaa ggtggctcct
      481 acaaatgcca tcattgcgat aaaggaaagg ccatcgttga agatgcctct gccgacagtg
      541 gtcccaaaga tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca
      601 cgtcttcaaa gcaagtggat tgatgtgata tcaccactga cgtaagggat gacgcacaat
      661 cccactatcc ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagaa
      721 cacgggggac tctagcgcta ccggttggct aggtaagctt ggtaccacct gaacgacgca
      781 tatgatctaa gctagcccca ccatggatcc aaaaaagaag agaaaggtag atccaaaaaa
      841 gaagagaaag gtaatggatg ctaagtcact aactgcctgg tcccggacac tggtgacctt
      901 caaggatgta tttgtggact tcaccaggga ggagtggaag ctgctggaca ctgctcagca
      961 gatcgtgtac agaaatgtga tgctggagaa ctataagaac ctggtttcct tgggttatca
     1021 gcttactaag ccagatgtga tcctccggtt ggagaaggga gaagagccct ggctggtgga
     1081 gagagaaatt caccaagaga cccatcctga ttcagagact gcatttgaaa tcaaatcatc
     1141 agtttccagc aggagcattt ttaaagataa gcaatcctgt gacattaaaa tggaaggaat
     1201 ggcaaggaat gatctctggg aattcggggc agacgacaca cgcgttgagg tgcaaccgcc
     1261 ggcgcagtgg gtcctcgacc tgatcgaggc cagcccgatc gcatcggtcg tgtccgatcc
     1321 gcgtctcgcc gacaatccgc tgatcgccat caaccaggcc ttcaccgacc tgaccggcta
     1381 ttccgaagaa gaatgcgtcg gccgcaattg ccgattcctg gcaggttccg gcaccgagcc
     1441 gtggctgacc gacaagatcc gccaaggcgt gcgcgagcac aagccggtgc tggtcgagat
     1501 cctgaactac aagaaggacg gcacgccgtt ccgcaatgcc gtgctcgttg caccgatcta
     1561 cgatgacgac gacgagcttc tctatttcct cggcagccag gtcgaagtcg acgacgacca
     1621 gcccaacatg ggcatggcgc gccgcgaacg cgccgcggaa atgctcaaga cgctgtcgcc
     1681 gcgccagctc gaggttacga cgctggtggc atcgggcttg cgcaacaagg aagtggcggc
     1741 ccggctcggc ctgtcggaga aaaccgtcaa gatgcaccgc gggctggtga tggaaaagct
     1801 caacctgaag accagtgccg atctggtgcg cattgccgtc gaagccggaa tctaaactag
     1861 ataagtaatg atcataatca gccatatcac atctgtagag gttttacttg ctttaaaaaa
     1921 cctcccacac ctcccgaatt ccccgggctc gagaagcttg gatccaccgg atctagataa
     1981 ctgatctcga ggcgaatttc cccgatcgtt caaacatttg gcaataaagt ttcttaagat
     2041 tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc
     2101 atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag
     2161 tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata
     2221 aattatcgcg cgcggtgtca tctatgttac tagatcggga attaattcga taagctagag
     2281 atccgtcaag gtacccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta
     2341 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     2401 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     2461 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     2521 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     2581 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     2641 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     2701 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     2761 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     2821 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     2881 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     2941 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     3001 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     3061 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     3121 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     3181 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     3241 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     3301 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     3361 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     3421 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     3481 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     3541 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     3601 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     3661 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     3721 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     3781 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     3841 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     3901 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     3961 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     4021 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     4081 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     4141 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     4201 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     4261 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     4321 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     4381 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     4441 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa
     4501 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     4561 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     4621 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     4681 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     4741 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     4801 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     4861 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     4921 cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac
     4981 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
     5041 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
     5101 acgacggcca gtgaattgta atacgactca ctatagggcg aattggagct ccaccgcgga
     5161 gcggagaatt aagggagtca ggccttaatt aagagctcgc atgcctgcag gtccccagat
     5221 tagccttttc aatttcagaa agaatgctaa cccacagatg gttagagagg cttacgcagc
     5281 aggtctcatc aagacgatct acccgagcaa taatctccag gaaatca
//
