LOCUS       pROF051                 4949 bp    DNA     circular UNA 24-JUN-2020
DEFINITION  PCaMV35S-SRDX-NLS-EL222-Tnos.
ACCESSION   urn.local...1h-bxngw4e
VERSION     urn.local...1h-bxngw4e
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     terminator      23..275
                     /note="nopaline synthase terminator and poly(A) signal"
                     /label="Tnos"
     primer_bind     complement(327..347)
                     /created_by="primer3"
                     /annotation_group="368498081522160f56bf3d8-15a9-4f55-8207-
                     100fb1c7acab"
                     /%GC="42.9"
                     /Tm="54.5"
                     /Hairpin_Tm="36.1"
                     /Self_Dimer_Tm="None"
                     /Sequence="GCAATTAACCCTCACTAAAGG"
                     /Transferred_From="T3 "
                     /Transferred_Similarity="100.00%"
                     /label="T3 "
     primer_bind     complement(379..401)
                     /created_by="primer3"
                     /annotation_group="3681122420212641c0712f0-c42c-4e1e-a842-
                     656678a279c5"
                     /%GC="43.5"
                     /Tm="59.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="AGCGGATAACAATTTCACACAGG"
                     /Transferred_From="M13/pUC Reverse"
                     /Transferred_Similarity="100.00%"
                     /label="M13/pUC Reverse"
     rep_origin      complement(768..1356)
                     /direction="LEFT; RIGHT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /label="ori"
     primer_bind     complement(848..867)
                     /created_by="primer3"
                     /annotation_group="368013014609700e515e0e9-9633-4f68-b574-
                     2e898e0c518f"
                     /%GC="50.0"
                     /Tm="57.9"
                     /Hairpin_Tm="36.7"
                     /Self_Dimer_Tm="None"
                     /Sequence="GGGAAACGCCTGGTATCTTT"
                     /Transferred_From="pBR322ori-F"
                     /Transferred_Similarity="100.00%"
                     /label="pBR322ori-F"
     CDS             order(complement(2319..2387),complement(1527..2318))
                     /label="AmpR"
     primer_bind     2150..2169
                     /created_by="primer3"
                     /annotation_group="368315469795102f7820b24-c956-49ef-8962-
                     5734406f9c9f"
                     /%GC="50.0"
                     /Tm="58.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="ATAATACCGCGCCACATAGC"
                     /X-Transferred_From="Amp-R "
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pROF021"
                     /Transferred_Similarity="100.00%"
                     /label="Amp-R "
     promoter        complement(2388..2492)
                     /label="AmpR promoter"
     rep_origin      complement(2518..2973)
                     /direction="LEFT"
                     /note="arrow  indicates direction of (+) strand synthesis;
                     f1 bacteriophage origin of replication"
                     /label="f1 ori"
     primer_bind     complement(2655..2676)
                     /created_by="primer3"
                     /annotation_group="3684274549334999fa52d5c-da05-437b-9cd0-
                     d103c403e83b"
                     /%GC="50.0"
                     /Tm="59.4"
                     /Hairpin_Tm="39.7"
                     /Self_Dimer_Tm="None"
                     /Sequence="GTGGACTCTTGTTCCAAACTGG"
                     /Transferred_From="F1ori-F "
                     /Transferred_Similarity="100.00%"
                     /label="F1ori-F "
     primer_bind     3100..3122
                     /X-Transferred_From="M13/pUC Forward"
                     /X-Transferred_Similarity="100.00%"
                     /Sequence="CCCAGTCACGACGTTGTAAAACG"
                     /Hairpin_Tm="50.0"
                     /Tm="62.4"
                     /Self_Dimer_Tm="16.6"
                     /%GC="52.2"
                     /modified_by="User"
                     /label="M13/pUC Forward"
     primer_bind     3138..3157
                     /created_by="primer3"
                     /annotation_group="368513478869144dcf9d8a1-f8f6-4d2c-8401-
                     e956510671a8"
                     /%GC="40.0"
                     /Tm="50.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TAATACGACTCACTATAGGG"
                     /Transferred_From="T7"
                     /Transferred_Similarity="100.00%"
                     /label="T7"
     promoter        3204..4077
                     /label="PCaMV35S "
     origin          3346^3347
                     /label="origin"
     primer_bind     4011..4031
                     /created_by="primer3"
                     /annotation_group="368344346793449b2fd01e7-579d-418a-a8e8-
                     98348d47a09d"
                     /%GC="52.4"
                     /Tm="58.2"
                     /Hairpin_Tm="53.1"
                     /Self_Dimer_Tm="None"
                     /Sequence="CTATCCTTCGCAAGACCCTTC"
                     /Transferred_From="35S promoter "
                     /Transferred_Similarity="100.00%"
                     /label="35S promoter "
     ORF             4128..4823
                     /Genetic_code="Standard"
                     /Reading_frame=3
                     /created_by="ORF Finder"
                     /modified_by="User"
                     /label="SRDX-NLS-EL222"
     CDS             4131..4160
                     /modified_by="User"
                     /label="SRDX"
     sig_peptide     4161..4181
                     /modified_by="User"
                     /vntifkey=21
                     /Transferred_From="BM00654 (reversed), pROF141"
                     /Transferred_Similarity="100.00%"
                     /label="NLS"
     misc_feature    4182..4193
                     /label="Linker"
     CDS             4194..4820
                     /label="EL222"
ORIGIN      
        1 tgatctcgag gcgaatttcc ccgatcgttc aaacatttgg caataaagtt tcttaagatt
       61 gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca
      121 tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt
      181 cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa
      241 attatcgcgc gcggtgtcat ctatgttact agatcgggaa ttaattcgat aagctagaga
      301 tccgtcaagg tacccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa
      361 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
      421 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
      481 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
      541 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
      601 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
      661 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
      721 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
      781 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
      841 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
      901 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
      961 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     1021 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     1081 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     1141 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     1201 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     1261 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     1321 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     1381 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     1441 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     1501 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     1561 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     1621 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     1681 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     1741 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     1801 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     1861 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     1921 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     1981 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     2041 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     2101 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     2161 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     2221 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     2281 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     2341 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     2401 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     2461 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctaaa
     2521 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     2581 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     2641 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     2701 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     2761 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     2821 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     2881 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     2941 ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg ctgcgcaact
     3001 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
     3061 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
     3121 cgacggccag tgaattgtaa tacgactcac tatagggcga attggagctc caccgcggag
     3181 cggagaatta agggagtcag gccttaatta agagctcgca tgcctgcagg tccccagatt
     3241 agccttttca atttcagaaa gaatgctaac ccacagatgg ttagagaggc ttacgcagca
     3301 ggtctcatca agacgatcta cccgagcaat aatctccagg aaatcaaata ccttcccaag
     3361 aaggttaaag atgcagtcaa aagattcagg actaactgca tcaagaacac agagaaagat
     3421 atatttctca agatcagaag tactattcca gtatggacga ttcaaggctt gcttcacaaa
     3481 ccaaggcaag taatagagat tggagtctct aaaaaggtag ttcccactga atcaaaggcc
     3541 atggagtcaa agattcaaat agaggaccta acagaactcg ccgtaaagac tggcgaacag
     3601 ttcatacaga gtctcttacg actcaatgac aagaagaaaa tcttcgtcaa catggtggag
     3661 cacgacacac ttgtctactc caaaaatatc aaagatacag tctcagaaga ccaaagggca
     3721 attgagactt ttcaacaaag ggtaatatcc ggaaacctcc tcggattcca ttgcccagct
     3781 atctgtcact ttattgtgaa gatagtggaa aaggaaggtg gctcctacaa atgccatcat
     3841 tgcgataaag gaaaggccat cgttgaagat gcctctgccg acagtggtcc caaagatgga
     3901 cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa
     3961 gtggattgat gtgatatcac cactgacgta agggatgacg cacaatccca ctatccttcg
     4021 caagaccctt cctctatata aggaagttca tttcatttgg agagaacacg ggggactcta
     4081 gcgctaccgg ttggctaggt aagcttggta ccacctgaac gacgcatatg cttgatcttg
     4141 aacttagact tggttttgct ccaaagaaga agaggaaggt gggagctgga gctggggcag
     4201 acgacacacg cgttgaggtg caaccgccgg cgcagtgggt cctcgacctg atcgaggcca
     4261 gcccgatcgc atcggtcgtg tccgatccgc gtctcgccga caatccgctg atcgccatca
     4321 accaggcctt caccgacctg accggctatt ccgaagaaga atgcgtcggc cgcaattgcc
     4381 gattcctggc aggttccggc accgagccgt ggctgaccga caagatccgc caaggcgtgc
     4441 gcgagcacaa gccggtgctg gtcgagatcc tgaactacaa gaaggacggc acgccgttcc
     4501 gcaatgccgt gctcgttgca ccgatctacg atgacgacga cgagcttctc tatttcctcg
     4561 gcagccaggt cgaagtcgac gacgaccagc ccaacatggg catggcgcgc cgcgaacgcg
     4621 ccgcggaaat gctcaagacg ctgtcgccgc gccagctcga ggttacgacg ctggtggcat
     4681 cgggcttgcg caacaaggaa gtggcggccc ggctcggcct gtcggagaaa accgtcaaga
     4741 tgcaccgcgg gctggtgatg gaaaagctca acctgaagac cagtgccgat ctggtgcgca
     4801 ttgccgtcga agccggaatc taaactagat aagtaatgat cataatcagc catatcacat
     4861 ctgtagaggt tttacttgct ttaaaaaacc tcccacacct cccgaattcc ccgggctcga
     4921 gaagcttgga tccaccggat ctagataac
//
