LOCUS       pROF402                 5653 bp    DNA     circular UNA 15-JUN-2020
DEFINITION  P35Senhancer(-953 to -51)-(C120)5-PhCMVmin-FLuc-T35S .
ACCESSION   urn.local...3a-bxnh4rc
VERSION     urn.local...3a-bxnh4rc
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     region          complement(17..41)
                     /label="Right Border"
     rep_origin      complement(132..720)
                     /direction="LEFT; RIGHT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /label="ori"
     primer_bind     complement(212..231)
                     /created_by="primer3"
                     /annotation_group="368013014609700e515e0e9-9633-4f68-b574-
                     2e898e0c518f"
                     /%GC="50.0"
                     /Tm="57.9"
                     /Hairpin_Tm="36.7"
                     /Self_Dimer_Tm="None"
                     /Sequence="GGGAAACGCCTGGTATCTTT"
                     /Transferred_From="pBR322ori-F"
                     /Transferred_Similarity="100.00%"
                     /label="pBR322ori-F"
     modified_base   442
                     /created_by="User"
                     /label="G>T"
     CDS             complement(891..1706)
                     /label="KanR"
     modified_base   1516
                     /created_by="User"
                     /label="A>T"
     rep_origin      order(1997..2025,2026..2432)
                     /label="pSa ori"
     misc_feature    2555^2556
                     /Original_Bases="GA"
                     /label="GA"
                     /note="Geneious type: Editing History Deletion"
     region          2559..2581
                     /label="Left Border"
     origin          2578^2579
                     /label="origin"
     promoter        2604..3506
                     /modified_by="User"
                     /label="P35Senhancer(-953 to -51)"
     misc_feature    3366
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
     repeat_region   3523..3642
                     /label="(C120)5"
     promoter        3661..3760
                     /label="PhCMVmin"
     CDS             3768..5420
                     /modified_by="User"
                     /label="FLuc"
     primer_bind     complement(3834..3852)
                     /created_by="primer3"
                     /annotation_group="3682505912022276d08537f-025a-4a9b-b0c8-
                     7f12defb0af5"
                     /%GC="52.6"
                     /Tm="55.7"
                     /Hairpin_Tm="42.7"
                     /Self_Dimer_Tm="5.6"
                     /Sequence="CCTTATGCAGTTGCTCTCC"
                     /Transferred_From="LucNrev"
                     /Transferred_Similarity="100.00%"
                     /label="LucNrev"
     primer_bind     5277..5295
                     /created_by="primer3"
                     /annotation_group="368241913672039eacbda21-e817-4307-8cac-
                     6a33aa3f0f52"
                     /%GC="47.4"
                     /Tm="57.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="8.2"
                     /Sequence="AGTCAAGTAACAACCGCGA"
                     /Transferred_From="Luc-F"
                     /Transferred_Similarity="100.00%"
                     /label="Luc-F"
     terminator      5428..5653
                     /modified_by="User"
                     /label="T35S"
ORIGIN      
        1 cgcttgagac gaagcttgac aggatatatt ggcgggtaaa ctaagtcgct gtatgtgttt
       61 gtttgagatc tcatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
      121 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
      181 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
      241 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
      301 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
      361 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
      421 ttatccggta actatcgtct ttagtccaac ccggtaagac acgacttatc gccactggca
      481 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
      541 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
      601 aagccagtta ccttcggaag aagagttggt agctcttgat ccggcaaaca aaccaccgct
      661 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
      721 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
      781 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      841 tgaagtttta aatcaatcta aagtatatat gtgtaacatt ggtctagtga ttagaaaaac
      901 tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt
      961 tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca
     1021 agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc
     1081 ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt
     1141 gagaatggca aaagtttatg catttctttc cagacttgtt caacaggcca gccattacgc
     1201 tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg
     1261 agtcgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg
     1321 cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat
     1381 acctggaatg ctgttttccc tgggatcgca gtggtgagta accatgcatc atcaggagta
     1441 cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc
     1501 atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc
     1561 gcatcgggct tcccatacaa tcggtagatt gtcgcacctg attgcccgac attatcgcga
     1621 gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg ccttgagcaa
     1681 gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat gtaagcagac
     1741 agttttattg ttcatgatga tatattttta tcttgtgcaa tgtaacatca gagattttga
     1801 gacacaacgt ggctttgttg aataaatcga acttttgctg agttgaagga tcagatcacg
     1861 catcttcccg acaacgcaga ccgttccgtg gcaaagcaaa agttcaaaat caccaactgg
     1921 tccacctaca acaaagctct catcaaccgt ggctccctca ctttctggct ggatgatggg
     1981 gcgattcagg cgatccccat ccaacagccc gccgtcgagc gggctttttt atccccggaa
     2041 gcctgtggat agagggtagt tatccacgtg aaaccgctaa tgccccgcaa agccttgatt
     2101 cacggggctt tccggcccgc tccaaaaact atccacgtga aatcgctaat cagggtacgt
     2161 gaaatcgcta atcggagtac gtgaaatcgc taataaggtc acgtgaaatc gctaatcaaa
     2221 aaggcacgtg agaacgctaa tagccctttc agatcaacag cttgcaaaca cccctcgctc
     2281 cggcaagtag ttacagcaag tagtatgttc aattagcttt tcaattatga atatatatat
     2341 caattattgg tcgcccttgg cttgtggaca atgcgctacg cgcaccggct ccgcccgtgg
     2401 acaaccgcaa gcggttgccc accgtcgagc gcctttgccc acaacccggc ggccggccgc
     2461 aacagatcgt tttataaatt tttttttttg aaaaagaaaa agcccgaaag gcggcaacct
     2521 ctcgggcttc tggatttccg atccccggaa ttagatcttg gcaggatata ttgtggtgta
     2581 acaagcttcg tctcagtcag gagactagag ccaagctgat ctcctttgcc ccggagatca
     2641 ccatggacga ctttctctat ctctacgatc taggaagaaa gttcgacgga gaaggtgacg
     2701 ataccatgtt caccaccgat aatgagaaga ttagcctctt caatttcaga aagaatgctg
     2761 acccacagat ggttagagag gcctacgcgg caggtctgat caagacgatc tacccgagta
     2821 ataatctcca ggagatcaaa taccttccca agaaggttaa agatgcagtc aaaagattca
     2881 ggactaactg catcaagaac acagagaaag atatatttct caagatcaga agtactattc
     2941 cagtatggac gattcaaggc ttgcttcata aaccaaggca agtaatagag attggagtct
     3001 ctaagaaagt agttcctact gaatcaaagg ccatggagtc aaaaattcag atcgaggatc
     3061 taacagaact cgccgtgaag actggcgaac agttcataca gagtctttta cgactcaatg
     3121 acaagaagaa aatcttcgtc aacatggtgg agcacgacac tctcgtctac tccaagaata
     3181 tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa agggtaatat
     3241 cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa aggacagtag
     3301 aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct atcgttcaag
     3361 atgcccctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa
     3421 aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg
     3481 taagggatga cgcacaatcc cactattgac gctagcctcg agtaggtagc ctttagtcca
     3541 tgcgttatag gtagccttta gtccatgcgt tataggtagc ctttagtcca tgcgttatag
     3601 gtagccttta gtccatgcgt tataggtagc ctttagtcca tgaagctttc ccgcggccgc
     3661 cctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct
     3721 gttttgacct ccatagaaga caccgggacc gatccagcct accggtaatg gaagacgcca
     3781 aaaacataaa gaaaggcccg gcgccattct atccgctgga agatggaacc gctggagagc
     3841 aactgcataa ggctatgaag agatacgccc tggttcctgg aacaattgct tttacagatg
     3901 cacatatcga ggtggacatc acttacgctg agtacttcga aatgtccgtt cggttggcag
     3961 aagctatgaa acgatatggg ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact
     4021 ctcttcaatt ctttatgccg gtgttgggcg cgttatttat cggagttgca gttgcgcccg
     4081 cgaacgacat ttataatgaa cgtgaattgc tcaacagtat gggcatttcg cagcctaccg
     4141 tggtgttcgt ttccaaaaag gggttgcaaa aaattttgaa cgtgcaaaaa aagctcccaa
     4201 tcatccaaaa aattattatc atggattcta aaacggatta ccagggattt cagtcgatgt
     4261 acacgttcgt cacatctcat ctacctcccg gttttaatga atacgatttt gtgccagagt
     4321 ccttcgatag ggacaagaca attgcactga tcatgaactc ctctggatct actggtctgc
     4381 ctaaaggtgt cgctctgcct catagaactg cctgcgtgag attctcgcat gccagagatc
     4441 ctatttttgg caatcaaatc attccggata ctgcgatttt aagtgttgtt ccattccatc
     4501 acggttttgg aatgtttact acactcggat atttgatatg tggatttcga gtcgtcttaa
     4561 tgtatagatt tgaagaagag ctgtttctga ggagccttca ggattacaag attcaaagtg
     4621 cgctgctggt gccaacccta ttctccttct tcgccaaaag cactctgatt gacaaatacg
     4681 atttatctaa tttacacgaa attgcttctg gtggcgctcc cctctctaag gaagtcgggg
     4741 aagcggttgc caagaggttc catctgccag gtatcaggca aggatatggg ctcactgaga
     4801 ctacatcagc tattctgatt acacccgagg gggatgataa accgggcgcg gtcggtaaag
     4861 ttgttccatt ttttgaagcg aaggttgtgg atctggatac cgggaaaacg ctgggcgtta
     4921 atcaaagagg cgaactgtgt gtgagaggtc ctatgattat gtccggttat gtaaacaatc
     4981 cggaagcgac caacgccttg attgacaagg atggatggct acattctgga gacatagctt
     5041 actgggacga agacgaacac ttcttcatcg ttgaccgcct gaagtctctg attaagtaca
     5101 aaggctatca ggtggctccc gctgaattgg aatccatctt gctccaacac cccaacatct
     5161 tcgacgcagg tgtcgcaggt cttcccgacg atgacgccgg tgaacttccc gccgccgttg
     5221 ttgttttgga gcacggaaag acgatgacgg aaaaagagat cgtggattac gtcgccagtc
     5281 aagtaacaac cgcgaaaaag ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag
     5341 gtcttaccgg aaaactcgac gcaagaaaaa tcagagagat cctcataaag gccaagaagg
     5401 gcggaaagat cgccgtgtaa tgagcttcgg ccatgctaga gtccgcaaaa atcaccagtc
     5461 tctctctaca aatctatctc tctctatttt tctccagaat aatgtgtgag tagttcccag
     5521 ataagggaat tagggttctt atagggtttc gctcatgtgt tgagcatata agaaaccctt
     5581 agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa
     5641 aatccagtga cct
//
