LOCUS       BM00369                17759 bp ds-DNA     circular     04-JUN-2020
DEFINITION  Tnos-nptII-Pnos_Tnos-NLS-PIF6-E-PCaMV35S_Tnos
            -dsRed-PAtUbi10_Tnos-NLS-VP16-PhyB-PCaMV35S_Tnos-EL222-NLS-SRDX-PCa
            MV35S_T35S-GUS-PhCMVmin-(C120)5-(etr)8.
ACCESSION   urn.local...4x-bxnhb80
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
COMMENT     ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     repeat_region   24..310
                     /label="(etr)8"
     repeat_region   326..445
                     /label="(C120)5"
     promoter        468..567
                     /label="PhCMVmin"
     exon            569..815
                     /modified_by="User"
                     /label="GUS_Exon1"
     intron          816..900
                     /modified_by="User"
                     /label="Intron1"
     exon            901..1283
                     /modified_by="User"
                     /label="GUS_Exon2"
     intron          1284..1387
                     /modified_by="User"
                     /label="Intron2"
     exon            1388..2584
                     /modified_by="User"
                     /label="GUS_Exon3"
     terminator      2589..2792
                     /modified_by="User"
                     /label="T35S"
     promoter        2818..3242
                     /modified_by="User"
                     /label="PCaMV35S(short)"
     ORF             3247..3942
                     /Genetic_code="Standard"
                     /Reading_frame=1
                     /created_by="ORF Finder"
                     /modified_by="User"
                     /label="SRDX-NLS-EL222"
     CDS             3250..3279
                     /label="SRDX"
     sig_peptide     3280..3300
                     /label="NLS"
     misc_feature    3301..3312
                     /label="Linker"
     CDS             3313..3939
                     /label="EL222"
     terminator      3947..4209
                     /modified_by="User"
                     /label="Tnos"
     promoter        4235..4659
                     /modified_by="User"
                     /label="PCaMV35S(short)"
     ORF             4670..7066
                     /Genetic_code="Standard"
                     /Reading_frame=2
                     /created_by="ORF Finder"
                     /modified_by="User"
                     /label="PhyB-VP16-NLS"
     CDS             4670..6619
                     /modified_by="User"
                     /label="PhyB(1-650)"
     misc_feature    6629..6655
                     /modified_by="User"
                     /label="Linker"
     CDS             6656..7042
                     /modified_by="User"
                     /label="VP16"
     sig_peptide     7043..7063
                     /label="NLS"
     terminator      7071..7333
                     /modified_by="User"
                     /label="Tnos"
     promoter        7358..8868
                     /Note="no BsaI or BpiI sites "
                     /modified_by="User"
                     /label="PAtUbi10(domesticated)"
     modified_base   8658
                     /created_by="User"
                     /label="A>T"
     CDS             8870..9547
                     /Note="no BsaI or BpiI sites"
                     /modified_by="User"
                     /label="dsRed(domesticated)"
     terminator      9552..9814
                     /modified_by="User"
                     /label="Tnos"
     promoter        9840..10264
                     /modified_by="User"
                     /label="PCaMV35S(short)"
     ORF             10275..11228
                     /Genetic_code="Standard"
                     /Reading_frame=3
                     /created_by="ORF Finder"
                     /modified_by="User"
                     /label="E-PIF6-NLS"
     CDS             10275..10868
                     /label="E"
     misc_feature    10878..10904
                     /label="Linker"
     CDS             10905..11204
                     /label="PIF6(1-100)"
     sig_peptide     11205..11225
                     /modified_by="User"
                     /vntifkey=21
                     /X-Transferred_From="BM00654 (reversed); pMZ828; pROF050;
                     pROF051; pROF141"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="BM00654 (reversed), pMZ827, pMZ828,
                     pROF050, pROF051, pROF141, pROF148"
                     /Transferred_Similarity="100.00%"
                     /label="NLS"
     terminator      11233..11495
                     /modified_by="User"
                     /label="Tnos"
     promoter        11520..11795
                     /modified_by="User"
                     /label="Pnos"
     primer_bind     11679..11698
                     /created_by="primer3"
                     /annotation_group="3682127303365308a03fcb8-f47c-483a-aa1b-
                     5e7f6d01748b"
                     /%GC="50.0"
                     /Tm="57.8"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GCGTTCAAAAGTCGCCTAAG"
                     /Transferred_From="NOS-F "
                     /Transferred_Similarity="100.00%"
                     /label="NOS-F "
     CDS             11797..12591
                     /modified_by="User"
                     /label="nptII"
     terminator      12596..12858
                     /modified_by="User"
                     /label="Tnos"
     region          complement(12953..12975)
                     /label="Left Border"
     rep_origin      join(13047..15292,15293..15831)
                     /X-Transferred_From="EC50505\pL2V-1"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="User"
                     /label="pVS1ori(short)"
     CDS             13239..13868
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /X-Transferred_Translation="MKVIAVLNQKGGSGKTTIATHLARALQLAG
                     ADVLLVDSDPQGSARDWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAA
                     DLAVSAIKAADFVLIPVQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGT
                     RIGGEVAEALAGYELPILESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEI
                     KSKLI"
                     /X-Transferred_From="Xpre2-S; pCambia-addgene"
                     /modified_by="User"
                     /label="pVS1 StaA"
     CDS             14297..15369
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid
                      pVS1 (Heeb et al., 2000)"
                     /X-Transferred_Translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAA
                     QRQRIEAEARPGESWQAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVG
                     TRDSRSIMDVAVFRLSKRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVS
                     HLTESMNRYREGKGDKPGRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAM
                     QRTKKAKNGRLVTVSEGEALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVL
                     TVHPDYFLIDPGIGRFLYRLARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLI
                     GSNDLPEYDLKEEAGQAGPILVMRYRNLIEGEASAGS"
                     /X-Transferred_From="Xpre2-S"
                     /label="pVS1 RepA"
     rep_origin      15435..15629
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /X-Transferred_From="Xpre2-S"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="User"
                     /label="pVS1 oriV"
     promoter        15912..16016
                     /label="AmpR promoter"
     CDS             16017..16808
                     /Note="no BpiI sites"
                     /label="KanR(domesticated)"
     modified_base   16499
                     /created_by="User"
                     /label="A>G"
     rep_origin      16982..17570
                     /direction="LEFT; RIGHT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /label="ori"
     primer_bind     17471..17490
                     /created_by="primer3"
                     /annotation_group="368013014609700e515e0e9-9633-4f68-b574-
                     2e898e0c518f"
                     /%GC="50.0"
                     /Tm="57.9"
                     /Hairpin_Tm="36.7"
                     /Self_Dimer_Tm="None"
                     /Sequence="GGGAAACGCCTGGTATCTTT"
                     /Transferred_From="pBR322ori-F"
                     /Transferred_Similarity="100.00%"
                     /label="pBR322ori-F"
     region          17693..17717
                     /label="Right Border"
ORIGIN      
        1 gctcggagct tcgaagttta aacgattgaa tataaccgac gtgactgtta catttaggga
       61 ttgaatataa ccgacgtgac tgttacattt agggattgaa tataaccgac gtgactgtta
      121 catttaggga ttgaatataa ccgacgtgac tgttacattt agggataaac gattgaatat
      181 aaccgacgtg actgttacat ttagggattg aatataaccg acgtgactgt tacatttagg
      241 gattgaatat aaccgacgtg actgttacat ttagggattg aatataaccg acgtgactgt
      301 tacatttagg gatgctagcc tcgagtaggt agcctttagt ccatgcgtta taggtagcct
      361 ttagtccatg cgttataggt agcctttagt ccatgcgtta taggtagcct ttagtccatg
      421 cgttataggt agcctttagt ccatgaagct tagacactag agctagccct atataagcag
      481 agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca
      541 tagaagacac cgggaccgat ccagcctaat gggtcagtcc cttatgttac gtcctgtaga
      601 aaccccaacc cgtgaaatca aaaaactcga cggcctgtgg gcattcagtc tggatcgcga
      661 aaactgtgga attgatcagc gttggtggga aagcgcgtta caagaaagcc gggcaattgc
      721 tgtgccaggc agttttaacg atcagttcgc cgatgcagat attcgtaatt atgcgggcaa
      781 cgtctggtat cagcgcgaag tctttatacc gaaaggtaag tcttactctc tcttttttgg
      841 tctgtatttt taattttttg aagtatacta tttgtactga cgctaataat cttttttcag
      901 gttgggcagg ccagcgtatc gtgctgcgtt tcgatgcggt cactcattac ggcaaagtgt
      961 gggtcaataa tcaggaagtg atggagcatc agggcggcta tacgccattt gaagccgatg
     1021 tcacgccgta tgttattgcc gggaaaagtg tacgtatcac cgtttgtgtg aacaacgaac
     1081 tgaactggca gactatcccg ccgggaatgg tgattaccga cgaaaacggc aagaaaaagc
     1141 agtcttactt ccatgatttc tttaactatg ccggaatcca tcgcagcgta atgctctaca
     1201 ccacgccgaa cacctgggtg gacgatatca ccgtggtgac gcatgtcgcg caagactgta
     1261 accacgcgtc tgttgactgg caggtacttc atgcttcaac gtgtaactta agagatactg
     1321 tgtgaaattt tatatttcca tacatttgct tgacctttgc tttttgtcaa tttttttccc
     1381 cttacaggtg gtggccaatg gtgatgtcag cgttgaactg cgtgatgcgg atcaacaggt
     1441 ggttgcaact ggacaaggca ctagcgggac tttgcaagtg gtgaatccgc acctctggca
     1501 accgggtgaa ggttatctct atgaactgtg cgtcacagcc aaaagccaga cagagtgtga
     1561 tatctacccg cttcgcgtcg gcatccggtc agtggcagtg aagggcgaac agttcctgat
     1621 taaccacaaa ccgttctact ttactggctt tggtcgtcat gaagatgcgg acttgcgtgg
     1681 caaaggattc gataacgtgc tgatggtgca cgaccacgca ttaatggact ggattggggc
     1741 caactcctac cgtacctcgc attaccctta cgctgaagag atgctcgact gggcagatga
     1801 acatggcatc gtggtgattg atgaaactgc tgctgtcggc tttaacctct ctttaggcat
     1861 tggtttcgaa gcgggcaaca agccgaaaga actgtacagc gaagaggcag tcaacgggga
     1921 aactcagcaa gcgcacttac aggcgattaa agagctgata gcgcgtgaca aaaaccaccc
     1981 aagcgtggtg atgtggagta ttgccaacga accggatacc cgtccgcaag gtgcacggga
     2041 atatttcgcg ccactggcgg aagcaacgcg taaactcgac ccgacgcgtc cgatcacctg
     2101 cgtcaatgta atgttctgcg acgctcacac cgataccatc agcgatctct ttgatgtgct
     2161 gtgcctgaac cgttattacg gatggtatgt ccaaagcggc gatttggaaa cggcagagaa
     2221 ggtactggaa aaagaacttc tggcctggca ggagaaactg catcagccga ttatcatcac
     2281 cgaatacggc gtggatacgt tagccgggct gcactcaatg tacaccgaca tgtggagtga
     2341 agagtatcag tgtgcatggc tggatatgta tcaccgcgtc tttgatcgcg tcagcgccgt
     2401 cgtcggtgaa caggtatgga atttcgccga ttttgcgacc tcgcaaggca tattgcgcgt
     2461 tggcggtaac aagaaaggga tcttcactcg cgaccgcaaa ccgaagtcgg cggcttttct
     2521 gctgcaaaaa cgctggactg gcatgaactt cggtgaaaaa ccgcagcagg gaggcaaaca
     2581 atgagcttct ctagctagag tcgatcgaca agctcgagtt tctccataat aatgtgtgag
     2641 tagttcccag ataagggaat tagggttcct atagggtttc gctcatgtgt tgagcatata
     2701 agaaaccctt agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc
     2761 ctaaaaccaa aatccagtac taaaatccag atcgctctcg aggaattcca caggaggtca
     2821 acatggtgga gcacgacact ctggtctact ccaaaaatgt caaagataca gtctcagaag
     2881 atcaaagggc tattgagact tttcaacaaa ggataatttc gggaaacctc ctcggattcc
     2941 attgcccagc tatctgtcac ttcatcgaaa ggacagtaga aaaggaaggt ggctcctaca
     3001 aatgccatca ttgcgataaa ggaaaggcta tcattcaaga tctctctgcc gacagtggtc
     3061 ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagaggtt ccaaccacgt
     3121 ctacaaagca agtggattga tgtgacatct ccactgacgt aagggatgac gcacaatccc
     3181 actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggacac
     3241 gcaatgatgc ttgatcttga acttagactt ggttttgctc caaagaagaa gaggaaggtg
     3301 ggagctggag ctggggcaga cgacacacgc gttgaggtgc aaccgccggc gcagtgggtc
     3361 ctcgacctga tcgaggccag cccgatcgca tcggtcgtgt ccgatccgcg tctcgccgac
     3421 aatccgctga tcgccatcaa ccaggccttc accgacctga ccggctattc cgaagaagaa
     3481 tgcgtcggcc gcaattgccg attcctggca ggttccggca ccgagccgtg gctgaccgac
     3541 aagatccgcc aaggcgtgcg cgagcacaag ccggtgctgg tcgagatcct gaactacaag
     3601 aaggacggca cgccgttccg caatgccgtg ctcgttgcac cgatctacga tgacgacgac
     3661 gagcttctct atttcctcgg cagccaggtc gaagtcgacg acgaccagcc caacatgggc
     3721 atggcgcgcc gcgaacgcgc cgcggaaatg ctcaagacgc tgtcgccgcg ccagctcgag
     3781 gttacgacgc tggtggcatc gggcttgcgc aacaaggaag tggcggcccg gctcggcctg
     3841 tcggagaaaa ccgtcaagat gcaccgcggg ctggtgatgg aaaagctcaa cctgaagacc
     3901 agtgccgatc tggtgcgcat tgccgtcgaa gccggaatct aagcttgtca agcagatcgt
     3961 tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt
     4021 atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg
     4081 ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata
     4141 gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta
     4201 ctagatcgac gctgcatgcg aattctctgg gaggtcaaca tggtggagca cgacactctg
     4261 gtctactcca aaaatgtcaa agatacagtc tcagaagatc aaagggctat tgagactttt
     4321 caacaaagga taatttcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc
     4381 atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga
     4441 aaggctatca ttcaagatct ctctgccgac agtggtccca aagatggacc cccacccacg
     4501 aggagcatcg tggaaaaaga agaggttcca accacgtcta caaagcaagt ggattgatgt
     4561 gacatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc
     4621 tctatataag gaagttcatt tcatttggag aggacacgca atgacaacaa tggtttccgg
     4681 agtcgggggt agtggcggtg gccgtggcgg tggccgtggc ggagaagaag aaccgtcgtc
     4741 aagtcacact cctaataacc gaagaggagg agaacaagct caatcgtcgg gaacgaaatc
     4801 tctcagacca agaagcaaca ctgaatcaat gagcaaagca attcaacagt acaccgtcga
     4861 cgcaagactc cacgccgttt tcgaacaatc cggcgaatca gggaaatcat tcgactactc
     4921 acaatcactc aaaacgacga cgtacggttc ctctgtacct gagcaacaga tcacagctta
     4981 tctctctcga atccagcgag gtggttacat tcagcctttc ggatgtatga tcgccgtcga
     5041 tgaatccagt ttccggatca tcggttacag tgaaaacgcc agagaaatgt tagggattat
     5101 gcctcaatct gttcctactc ttgagaaacc tgagattcta gctatgggaa ctgatgtgag
     5161 atctttgttc acttcttcga gctcgattct actcgagcgt gctttcgttg ctcgagagat
     5221 taccttgtta aatccggttt ggatccattc caagaatact ggtaaaccgt tttacgccat
     5281 tcttcatagg attgatgttg gtgttgttat tgatttagag ccagctagaa ctgaagatcc
     5341 tgcgctttct attgctggtg ctgttcaatc gcagaaactc gcggttcgtg cgatttctca
     5401 gttacaggct cttcctggtg gagatattaa gcttttgtgt gacactgtcg tggaaagtgt
     5461 gagggacttg actggttatg atcgtgttat ggtttataag tttcatgaag atgagcatgg
     5521 agaagttgta gctgagagta aacgagatga tttagagcct tatattggac tgcattatcc
     5581 tgctactgat attcctcaag cgtcaaggtt cttgtttaag cagaaccgtg tccgaatgat
     5641 agtagattgc aatgccacac ctgttcttgt ggtccaggac gataggctaa ctcagtctat
     5701 gtgcttggtt ggttctactc ttagggctcc tcatggttgt cactctcagt atatggctaa
     5761 catgggatct attgcgtctt tagcaatggc ggttataatc aatggaaatg aagatgatgg
     5821 gagcaatgta gctagtggaa gaagctcgat gaggctttgg ggtttggttg tttgccatca
     5881 cacttcttct cgctgcatac cgtttccgct aaggtatgct tgtgagtttt tgatgcaggc
     5941 tttcggttta cagttaaaca tggaattgca gttagctttg caaatgtcag agaaacgcgt
     6001 tttgagaacg cagacactgt tatgtgatat gcttctgcgt gactcgcctg ctggaattgt
     6061 tacacagagt cccagtatca tggacttagt gaaatgtgac ggtgcagcat ttctttacca
     6121 cgggaagtat tacccgttgg gtgttgctcc tagtgaagtt cagataaaag atgttgtgga
     6181 gtggttgctt gcgaatcatg cggattcaac cggattaagc actgatagtt taggcgatgc
     6241 ggggtatccc ggtgcagctg cgttagggga tgctgtgtgc ggtatggcag ttgcatatat
     6301 cacaaaaaga gactttcttt tttggtttcg atctcacact gcgaaagaaa tcaaatgggg
     6361 aggcgctaag catcatccgg aggataaaga tgatgggcaa cgaatgcatc ctcgttcgtc
     6421 ctttcaggct tttcttgaag ttgttaagag ccggagtcag ccatgggaaa ctgcggaaat
     6481 ggatgcgatt cactcgctcc agcttattct gagagactct tttaaagaat ctgaggcggc
     6541 tatgaactct aaagttgtgg atggtgtggt tcagccatgt agggatatgg cgggggaaca
     6601 ggggattgat gagttaggtg aattcgatag tgctggtagt gctggtagtg ctggttccgc
     6661 gtacagccgc gcgcgtacga aaaacaatta cgggtctacc atcgagggcc tgctcgatct
     6721 cccggacgac gacgcccccg aagaggcggg gctggcggct ccgcgcctgt cctttctccc
     6781 cgcgggacac acgcgcagac tgtcgacggc ccccccgacc gatgtcagcc tgggggacga
     6841 gctccactta gacggcgagg acgtggcgat ggcgcatgcc gacgcgctag acgatttcga
     6901 tctggacatg ttgggggacg gggattcccc gggtccggga tttacccccc acgactccgc
     6961 cccctacggc gctctggata tggccgactt cgagtttgag cagatgttta ccgatgccct
     7021 tggaattgac gagtacggtg ggccaaagaa gaagaggaag gtgtaggctt gtcaagcaga
     7081 tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat
     7141 gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat
     7201 gacgttattt atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc
     7261 gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat
     7321 gttactagat cgacgctcca tgggaattcg taaggaggac gtcgttgtgg ttggtgcttt
     7381 ccttacattc tgagcctctt tccttctaat ccactcatct gcatcttctt gtgtccttac
     7441 taatacctca ttggttccaa attccctccc tttaagcacc agctcgtttc tgttcttcca
     7501 cagcctccca agtatccaag ggactaaagc ctccacattc ttcagatcag gatattcttg
     7561 tttaagatgt tgaactctat ggaggtttgt atgaactgat gatctaggac cggataagtt
     7621 cccttcttca tagcgaactt attcaaagaa tgttttgtgt atcattcttg ttacattgtt
     7681 attaatgaaa aaatattatt ggtcattgga ctgaacacga gtgttaaata tggaccaggc
     7741 cccaaataag atccattgat atatgaatta aataacaaga ataaatcgag tcaccaaacc
     7801 acttgccttt tttaacgaga cttgttcacc aacttgatac aaaagtcatt atcctatgca
     7861 aatcaataat catacaaaaa tatccaataa cactaaaaaa attaaaagaa atggataatt
     7921 tcacaatatg ttatacgata aagaagttac ttttccaaga aattcactga ttttataagc
     7981 ccacttgcat tagataaatg gcaaaaaaaa acaaaaagga aaagaaataa agcacgaaga
     8041 attctagaaa atacgaaata cgcttcaatg cagtgggacc cacggttcaa ttattgccaa
     8101 ttttcagctc caccgtatat ttaaaaaata aaacgataat gctaaaaaaa tataaatcgt
     8161 aacgatcgtt aaatctcaac ggctggatct tatgacgacc gttaaggaaa ttgtggttgt
     8221 cggacgaagt ccagtaataa acggcgtcaa agtggttgca gccggcacac acgagtcgtg
     8281 tttatcaact caaagcacaa atacttttcc tcaacctaaa aataaggcaa ttagccaaaa
     8341 acaactttgc gtgtaaacaa cgctcaatac acgtgtcatt ttattattag ctattgcttc
     8401 accgccttag ctttctcgtg acctagtcgt cctcgtcttt tcttcttctt cttctataaa
     8461 acaataccca aagagctctt cttcttcaca attcagattt caatttctca aaatcttaaa
     8521 aactttctct caattctctc taccgtgatc aaggtaaatt tctgtgttcc ttattctctc
     8581 aaaatcttcg attttgtttt cgttcgatcc caatttcgta tatgttcttt ggtttagatt
     8641 ctgttaatct tagatcgtag acgattttct gggtttgatc gttagatatc atcttaattc
     8701 tcgattaggg tttcatagat atcatccgat ttgttcaaat aatttgagtt ttgtcgaata
     8761 attactcttc gatttgtgat ttctatctag atctggtgtt agtttctagt ttgtgcgatc
     8821 gaatttgtcg attaatctga gtttttctga ttaacagatg cagatcttaa tggggtcatc
     8881 caagaatgtt atcaaggagt tcatgaggtt taaggttcgc atggaaggaa cggtcaatgg
     8941 gcacgagttt gaaatagaag gcgaaggaga ggggaggcca tacgaaggcc acaataccgt
     9001 aaagcttaag gtaaccaagg ggggaccttt gccatttgct tgggatattt tgtcaccaca
     9061 atttcagtat ggaagcaagg tatatgtcaa gcaccctgcc gacataccag actataaaaa
     9121 gctgtcattt cctgaaggat ttaaatggga aagggtcatg aactttgaag atggtggcgt
     9181 cgttactgta acccaggatt ccagtttgca ggatggctgt ttcatctaca aggtcaagtt
     9241 cattggcgtg aactttcctt ccgatggacc tgttatgcaa aagaaaacaa tgggctggga
     9301 agccagcact gagcgtttgt atcctcgtga tggcgtgttg aaaggagaga ttcataaggc
     9361 tctgaagctg aaagacggtg gtcattacct agttgaattc aaaagtattt acatggcaaa
     9421 gaagcctgtg cagctaccag ggtactacta tgttgactcc aaactggata taacaagcca
     9481 caacgaagat tatacaatcg ttgagcagta tgaaagaacc gagggacgcc accatctgtt
     9541 cctttaagct tgtcaagcag atcgttcaaa catttggcaa taaagtttct taagattgaa
     9601 tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt
     9661 aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc
     9721 gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt
     9781 atcgcgcgcg gtgtcatcta tgttactaga tcgacgctga gctcgaattc tagtggaggt
     9841 caacatggtg gagcacgaca ctctggtcta ctccaaaaat gtcaaagata cagtctcaga
     9901 agatcaaagg gctattgaga cttttcaaca aaggataatt tcgggaaacc tcctcggatt
     9961 ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag gtggctccta
    10021 caaatgccat cattgcgata aaggaaaggc tatcattcaa gatctctctg ccgacagtgg
    10081 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagagg ttccaaccac
    10141 gtctacaaag caagtggatt gatgtgacat ctccactgac gtaagggatg acgcacaatc
    10201 ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac
    10261 acgcaatgac aacaatgccc cgccccaagc tcaagtccga tgacgaggta ctcgaggccg
    10321 ccaccgtagt gctgaagcgt tgcggtccca tagagttcac gctcagcgga gtagcaaagg
    10381 aggtggggct ctcccgcgca gcgttaatcc agcgcttcac caaccgcgat acgctgctgg
    10441 tgaggatgat ggagcgcggc gtcgagcagg tgcggcatta cctgaatgcg ataccgatag
    10501 gcgcagggcc gcaagggctc tgggaatttt tgcaggtgct cgttcggagc atgaacactc
    10561 gcaacgactt ctcggtgaac tatctcatct cctggtacga gctccaggtg ccggagctac
    10621 gcacgcttgc gatccagcgg aaccgcgcgg tggtggaggg gatccgcaag cgactgcccc
    10681 caggtgctcc tgcggcagct gagttgctcc tgcactcggt catcgctggc gcgacgatgc
    10741 agtgggccgt cgatccggat ggtgagctag ctgatcatgt gctggctcag atcgctgcca
    10801 tcctgtgttt aatgtttccc gaacacgacg atttccaact cctccaggca catgcgtccg
    10861 cgtacagctt aattaacagt gctggtagtg ctggtagtgc tggtatgatg ttcttaccaa
    10921 ccgattattg ttgcaggtta agcgatcaag agtatatgga gcttgtgttt gagaatggcc
    10981 agattcttgc aaagggccaa agatccaacg tttctctgca taatcaacgt accaaatcga
    11041 tcatggattt gtatgaggca gagtataacg aggatttcat gaagagtatc atccatggtg
    11101 gtggtggtgc catcacaaat ctcggggaca cgcaggttgt tccacaaagt catgttgctg
    11161 ctgcccatga aacaaacatg ttggaaagca ataaacatgt tgacccaaag aagaagagga
    11221 aggtgtaggc ttgtcaagca gatcgttcaa acatttggca ataaagtttc ttaagattga
    11281 atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg
    11341 taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc
    11401 cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat
    11461 tatcgcgcgc ggtgtcatct atgttactag atcgacgcta agcttgaatt cttgcggaga
    11521 gcggagaatt aagggagtca cgttatgacc cccgccgatg acgcgggaca agccgtttta
    11581 cgtttggaac tgacagaacc gcaacgttga aggagccact cagccgcggg tttctggagt
    11641 ttaatgagct aagcacatac gtcagaaacc attattgcgc gttcaaaagt cgcctaaggt
    11701 cactatcagc tagcaaatat ttcttgtcaa aaatgctcca ctgacgttcc ataaattccc
    11761 ctcggtatcc aattagagtc tcatattcac tctcaaatga ttgaacaaga tggattgcac
    11821 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
    11881 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
    11941 gtcaagaccg acctgtccgg tgccctgaat gaactccaag acgaggcagc gcggctatcg
    12001 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
    12061 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
    12121 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
    12181 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgaggacg tactcggatg
    12241 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
    12301 gaactgttcg ccaggctcaa ggcgcggatg cccgacggcg aggatctcgt cgtgacccag
    12361 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
    12421 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
    12481 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
    12541 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcttgtcaa
    12601 gcagatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt
    12661 gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa
    12721 tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa
    12781 tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca
    12841 tctatgttac tagatcgacg ctggatccga attcggcagg gcccactgca tccaccccag
    12901 tacattaaaa acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt gtttacacca
    12961 caatatatcc tgccaccagc cagccaacag ctccccgacc ggcagctcgg cacaaaatca
    13021 ccactcgata caggcagccc atcagccgtg cggctgcatg aaatcctggc cggtttgtct
    13081 gatgccaagc tggcggcctg gccggccagc ttggccgctg aagaaaccga gcgccgccgt
    13141 ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg
    13201 atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc gctgtactta
    13261 accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc gccctgcaac
    13321 tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg
    13381 cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc
    13441 gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg
    13501 cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg gtgcagccaa
    13561 gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag cgcattgagg
    13621 tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca
    13681 tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt gagtcccgta
    13741 tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag
    13801 aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac
    13861 tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc taagtgccgg
    13921 ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag acacgccagc
    13981 catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga agatgtacgc
    14041 ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc agctaccaga
    14101 gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga ggcggcatgg
    14161 aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga ggaacgggcg
    14221 gttggccagg cgtaagcggc tgggttgtct gccggccctg caatggcact ggaaccccca
    14281 agcccgagga atcggcgtga cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg
    14341 ctgggtgatg acctggtgga gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc
    14401 gaggcagaag cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa
    14461 tcccggcaac cgccggcagc cggtgcgccg tcgattagga agccgcccaa gggcgacgag
    14521 caaccagatt ttttcgttcc gatgctctat gacgtgggca cccgcgatag tcgcagcatc
    14581 atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc
    14641 tacgagcttc cagacgggca cgtagaggtt tccgcagggc cggccggcat ggccagtgtg
    14701 tgggattacg acctggtact gatggcggtt tcccatctaa ccgaatccat gaaccgatac
    14761 cgggaaggga agggagacaa gcccggccgc gtgttccgtc cacacgttgc ggacgtactc
    14821 aagttctgcc ggcgagccga tggcggaaag cagaaagacg acctggtaga aacctgcatt
    14881 cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa cggccgcctg
    14941 gtgacggtat ccgagggtga agccttgatt agccgctaca agatcgtaaa gagcgaaacc
    15001 gggcggccgg agtacatcga gatcgagcta gctgattgga tgtaccgcga gatcacagaa
    15061 ggcaagaacc cggacgtgct gacggttcac cccgattact ttttgatcga tcccggcatc
    15121 ggccgttttc tctaccgcct ggcacgccgc gccgcaggca aggcagaagc cagatggttg
    15181 ttcaagacga tctacgaacg cagtggcagc gccggagagt tcaagaagtt ctgtttcacc
    15241 gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg
    15301 caggctggcc cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc
    15361 ggttcctaat gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga
    15421 aaaagcttct ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac
    15481 cggaacccgt acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg
    15541 actgatataa aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa
    15601 actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg
    15661 caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct
    15721 atcgcggccg ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc
    15781 ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac cgagctccca
    15841 tccccccagg ggctgcgccc ctcggccgcg aacggcctca ccccaaaaat ggcagcgctg
    15901 gccaattcgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
    15961 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatgg
    16021 ctaaaatgag aatatcaccg gaattgaaaa aactgatcga aaaataccgc tgcgtaaaag
    16081 atacggaagg aatgtctcct gctaaggtat ataagctggt gggagaaaat gaaaacctat
    16141 atttaaaaat gacggacagc cggtataaag ggaccaccta tgatgtggaa cgggaaaagg
    16201 acatgatgct atggctggaa ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc
    16261 atgatggctg gagcaatctg ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt
    16321 atgaagatga acaaagccct gaaaagatta tcgagctgta tgcggagtgc atcaggctct
    16381 ttcactccat cgacatatcg gattgtccct atacgaatag cttagacagc cgcttagccg
    16441 aattggatta cttactgaat aacgatctgg ccgatgtgga ttgcgaaaac tgggaagagg
    16501 acactccatt taaagatccg cgcgagctgt atgatttttt aaagacggaa aagcccgaag
    16561 aggaacttgt cttttcccac ggcgacctgg gagacagcaa catctttgtg aaagatggca
    16621 aagtaagtgg ctttattgat cttgggagaa gcggcagggc ggacaagtgg tatgacattg
    16681 ccttctgcgt ccggtcgatc agggaggata tcggggaaga acagtatgtc gagctatttt
    16741 ttgacttact ggggatcaag cctgattggg agaaaataaa atattatatt ttactggatg
    16801 aattgtttta gctgtcagac caagtttact catatatact ttagattgat ttaaaacttc
    16861 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
    16921 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
    16981 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
    17041 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
    17101 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
    17161 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
    17221 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
    17281 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
    17341 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
    17401 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
    17461 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
    17521 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
    17581 acgcggcctt tttacggttc ctgtggttgg cacatacaaa tggacgaacg gataaacctt
    17641 ttcacgccct tttaaatatc cgattattct aataaacgct cttttctctt aggtttaccc
    17701 gccaatatat cctgtcaaac actgatagtt taaactccct cggtcacatg tgcatcctc
//
