LOCUS       Exported                6660 bp ds-DNA     circular SYN 23-JUN-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jun 23, 2020 from SnapGene Viewer 5.1.4.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6660
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        17..526
                     /label=PGK promoter
                     /note="/vntifkey=30"
     misc_feature    558..1841
                     /label=coBpaRS1
     misc_feature    666..668
                     /label=37Gly
     misc_feature    1101..1103
                     /label=Gly182
     misc_feature    1104..1106
                     /label=183Phe
     misc_feature    1113..1115
                     /label=186Ala
     misc_feature    1892..2104
                     /label=SV40 polyA late
     misc_feature    2120..2377
                     /label=human U6 promoter
                     /label=human U6 promoter(3)
     misc_feature    2378..2459
                     /label=B. stearothermophilus Tyr-tRNA-CUA (BstYam)
     misc_feature    2412..2414
                     /label=amber
                     /label=amber(1)
     misc_feature    2460..2471
                     /label=3' termination sequence
                     /label=3' termination sequence(2)
     misc_feature    2478..2735
                     /label=human U6 promoter
                     /label=human U6 promoter(1)
     misc_feature    2736..2817
                     /label=B. stearothermophilus Tyr-tRNA-CUA
                     (BstYam)
                     /label=B. stearothermophilus Tyr-tRNA-CUA (BstYam)(3)
     misc_feature    2770..2772
                     /label=amber
     misc_feature    2818..2829
                     /label=3' termination sequence
                     /label=3' termination sequence(1)
     misc_feature    2836..3093
                     /label=human U6 promoter
                     /label=human U6 promoter(2)
     misc_feature    3094..3175
                     /label=B. stearothermophilus Tyr-tRNA-CUA
                     (BstYam)
                     /label=B. stearothermophilus Tyr-tRNA-CUA (BstYam)(1)
     misc_feature    3128..3130
                     /label=amber
                     /label=amber(3)
     misc_feature    3176..3187
                     /label=3' termination sequence
     misc_feature    3194..3451
                     /label=human U6 promoter
     misc_feature    3452..3533
                     /label=B. stearothermophilus Tyr-tRNA-CUA
                     (BstYam)
                     /label=B. stearothermophilus Tyr-tRNA-CUA (BstYam)(2)
     misc_feature    3486..3488
                     /label=amber
                     /label=amber(2)
     misc_feature    3534..3545
                     /label=3' termination sequence
                     /label=3' termination sequence(3)
     rep_origin      complement(3554..4008)
                     /direction=LEFT
                     /label=F1 ori
     rep_origin      complement(3686..3992)
                     /direction=LEFT
                     /label=F1 ori
                     /label=F1 ori(1)
     misc_feature    4071..4099
                     /label=Bacterial promoter for KAN
     promoter        4183..4499
                     /label=SV40 early promoter
     promoter        4303..4499
                     /label=SV40 early promoter
                     /label=SV40 early promoter(1)
     misc_feature    4350..4427
                     /label=SV40 Origin
     CDS             4534..5328
                     /label=Kan/neoR
     CDS             4537..5328
                     /label=Kan/neoR
                     /label=Kan/neoR(1)
     misc_feature    5522..5765
                     /label=TK PA terminator
     rep_origin      5882..6568
                     /label=pUC Origin
ORIGIN
        1 acgcgttagt tattaaaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga
       61 gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc
      121 acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg
      181 ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc
      241 aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct
      301 gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct
      361 ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg
      421 ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc
      481 acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg ctagcgttta
      541 aacttaagct tgccaccatg gccagcagca acctgatcaa gcagctgcag gaacggggcc
      601 tggtggccca agtgacagat gaagaagccc tggccgagag actggcccag ggacctattg
      661 ccctgggctg cggctttgac cccaccgccg attctctgca cctgggacat ctggtgcctc
      721 tgctgtgtct gaagcggttc cagcaggccg gccacaaacc tgtggctctc gtgggcggag
      781 ccaccggcct gattggcgat cctagcttta aggccgccga gcggaagctg aacaccgaag
      841 agacagtgca ggaatgggtg gacaagatcc ggaaacaggt ggcccccttc ctggacttcg
      901 actgcggcga gaatagcgcc attgccgcca acaactacga ttggttcggc aatatgaacg
      961 tgctgacctt cctgcgggac atcggcaagc acttcagcgt gaaccagatg atcaacaaag
     1021 aagccgtgaa gcagcggctg aacagagagg accagggcat cagcttcacc gagttcagct
     1081 acaacctgct gcagggctac ggcttcgcct gcgccaacaa gcagtacggc gtggtgctgc
     1141 agatcggcgg ctctgaccag tggggcaata tcaccagcgg catcgacctg accagacggc
     1201 tgcaccagaa ccaggtgttc ggcctgaccg tgcccctgat cacaaaggcc gacggcacca
     1261 agttcggcaa gaccgaaggc ggagccgtgt ggctggaccc caagaaaacc agcccctaca
     1321 agttctacca gttctggatc aacaccgcca gagccgacgt gtaccggttc ctgaagttct
     1381 tcaccttcat gagcatcgag gaaatcaacg ccctggaaga agaggacaag aacagcggca
     1441 aggcccccag agcccagtac gtgctggcag aacaagtgac caggctggtg cacggcgagg
     1501 aaggactgca ggccgccaag agaatcaccg agtgcctgtt cagcggcagc ctgagcgctc
     1561 tgagcgaggc cgattttgag cagctggctc aggacggcgt gcccatggtg gaaatggaaa
     1621 agggcgccga cctgatgcag gccctggtgg attctgagct gcagcccagc agaggacagg
     1681 ccagaaagac aatcgccagc aacgccatca ccatcaacgg cgagaagcag agcgaccccg
     1741 agtacttctt caaagaggaa gatcggctgt tcggccggtt caccctgctg cggagaggca
     1801 agaagaacta ctgcctgatc tgctggaagt aataagaatt ctgcagatat ccagcacagt
     1861 ggcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     1921 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     1981 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     2041 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     2101 cttaaggtct agactcgagg ggcaggaaga gggcctattt cccatgattc cttcatattt
     2161 gcatatacga tacaaggctg ttagagagat aattagaatt aatttgactg taaacacaaa
     2221 gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt
     2281 aaaattatgt tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt
     2341 cttggcttta tatatcttgt ggaaaggacg aaacaccgga ggggtagcga agtggctaaa
     2401 cgcggcggac tctaaatccg ctccctttgg gttcggcggt tcgaatccgt ccccctccag
     2461 tccttttttt ggtcgagggg caggaagagg gcctatttcc catgattcct tcatatttgc
     2521 atatacgata caaggctgtt agagagataa ttagaattaa tttgactgta aacacaaaga
     2581 tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa
     2641 aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct
     2701 tggctttata tatcttgtgg aaaggacgaa acaccggagg ggtagcgaag tggctaaacg
     2761 cggcggactc taaatccgct ccctttgggt tcggcggttc gaatccgtcc ccctccagtc
     2821 ctttttttgg tcgaggggca ggaagagggc ctatttccca tgattccttc atatttgcat
     2881 atacgataca aggctgttag agagataatt agaattaatt tgactgtaaa cacaaagata
     2941 ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa
     3001 ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg
     3061 gctttatata tcttgtggaa aggacgaaac accggagggg tagcgaagtg gctaaacgcg
     3121 gcggactcta aatccgctcc ctttgggttc ggcggttcga atccgtcccc ctccagtcct
     3181 ttttttggtc gaggggcagg aagagggcct atttcccatg attccttcat atttgcatat
     3241 acgatacaag gctgttagag agataattag aattaatttg actgtaaaca caaagatatt
     3301 agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt
     3361 atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc
     3421 tttatatatc ttgtggaaag gacgaaacac cggaggggta gcgaagtggc taaacgcggc
     3481 ggactctaaa tccgctccct ttgggttcgg cggttcgaat ccgtccccct ccagtccttt
     3541 ttttggtcga cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
     3601 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     3661 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     3721 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     3781 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     3841 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     3901 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     3961 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact
     4021 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     4081 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     4141 cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     4201 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     4261 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     4321 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     4381 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     4441 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag
     4501 atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     4561 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     4621 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     4681 aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg
     4741 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     4801 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     4861 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     4921 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     4981 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     5041 ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc
     5101 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     5161 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     5221 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     5281 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     5341 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     5401 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     5461 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa
     5521 cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata
     5581 aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact
     5641 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc
     5701 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag
     5761 gccctgccat agcagtacta aatatttatc ccgggctcag gttactcata tatactttag
     5821 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     5881 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     5941 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     6001 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     6061 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     6121 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     6181 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     6241 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     6301 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     6361 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     6421 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     6481 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     6541 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     6601 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat
//
