LOCUS       pJBJZ_003                 3021 bp    DNA     circular UNA 07-JUL-2020
DEFINITION  synthetic circular DNA
ACCESSION   Addgene ID:155358
VERSION     07/06/2020
KEYWORDS    pKD3, Rifampicin
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
FEATURES             Location/Qualifiers
     misc_feature    31..50
                     /note="priming site 1"
                     /standard_name="priming site 1"
     misc_feature    32..51
                     /note="priming site 3"
                     /standard_name="priming site 3"
     protein_bind    complement(51..98)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core
                     sequence TCTAGAAA (Turan and Bode, 2011)."
                     /note="Geneious type: misc_feature"
                     /standard_name="FRT"
     protein_bind    complement(51..84)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
                     /standard_name="FRT (minimal)"
     primer_bind     99..119
                     /standard_name="UR1"
                     /note="cf Roth lab"
     CDS             complement(358..810)
                     /note="confers resistance to Rifampicin"
                     /codon_start=1
                     /transl_table=11
                     /label=RifR
                     /standard_name="Rif R"
                     /standard_name="Rifampicin cassette"
     promoter        complement(873..899)
                     /standard_name="promoter Rif"
     primer_bind     complement(1092..1112)
                     /standard_name="UL1"
                     /note="cf Roth lab"
     misc_feature    1137..1156
                     /note="common priming site c2"
                     /standard_name="common priming site c2"
     protein_bind    complement(1163..1196)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"     misc_feature    complement(1246..1287)
                     /note="distal 42-nt of natural FRT site"
                     /standard_name="distal 42-nt of natural FRT site"
     misc_feature    complement(1163..1204)
                     /note="distal 42-nt of natural FRT site"
                     /standard_name="distal 42-nt of natural FRT site"
     misc_feature    1226..1245
                     /note="priming site 2"
                     /standard_name="priming site 2"
     rep_origin      1229..1627
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             complement(1734..2594)
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase"
                     /label=AmpR
                     /protein_id="AAL02034.1"
                     /db_xref="GI:15554331"
                     /standard_name="AmpR"
     promoter        complement(2595..2699)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      2789..2875
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2967..2994
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN      
        1 agattgcagc attacacgtc ttgagcgatt gtgtaggctg gagctgcttc gaagttccta
       61 tactttctag agaataggaa cttcggaata ggaacttcca cacaaccaca ccacaccaca
      121 cggggagagc ctgagcaaac tggcctcagg catttgagaa gcacacggtc acactgcttc
      181 cggtagtcaa taaaccggta aaccagcaat agacataagc ggctatttaa cgaccctgcc
      241 ctgaaccgac gaccgggtcg aatttgcttt cgaatttctg ccattcatcc gcttattatc
      301 acttattcag gcgtagcaac caggcgttta agggcaccaa taactgcctt aaaaaaacta
      361 gtcttcaatg acgtgtaaac cacggcgctt taagtcctct aacgaatcca acattcccct
      421 tattaattca acaggatgcc cctcccagtc ttcaacaacg ccaacaattc tcaagggttc
      481 gcaggttcta taggactgtg ttggattacc gggaaatttt ttgttcgtaa gattcggatc
      541 gtcttcgaac ggtcctgttg gctcaactat gtatatgtag ccgcgaccct cgaggccaga
      601 cagtgacata gcaagttcag ctccccaaac tgctggctcc atcaaggctg aaaagtagat
      661 gtgcttaaga atacgaccgt cctcgaaatg agagatgaac cctgtggtta gcaagtcacc
      721 aatcgccaaa ttggctttgg ttccatgata gaacggtcct tgcacctgct tgtaattatc
      781 atgagagatg ggaatccaat cttttaccat tttagctcct tagctcctga actcgataac
      841 tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg
      901 tgccgatcaa cgtctcattt tcgccaaaag ttggcccagg gcttcccggt atcaacaggg
      961 acaccaggat ttatttattc tgcgaagtga tcttccgtca caggtattta ttcggcgcaa
     1021 agtgcgtcgg gtgatgctgc caacttactg atttagtgta tgatggtgtt tttgaggtgc
     1081 tccagtggct tggttttggg gggtgtttgg tgcaggattt atttattctg cgaagtgatc
     1141 ttccgtcaca ggtaggcgcg ccgaagttcc tatactttct agagaatagg aacttcggaa
     1201 taggaactaa ggaggatatt catatggacc atggctaatt cccatgtcag ccgttaagtg
     1261 ttcctgtgtc actcaaaatt gctttgagag gctctaaggg cttctcagtg cgttacatcc
     1321 ctggcttgtt gtccacaacc gttaaacctt aaaagcttta aaagccttat atattctttt
     1381 tttcttataa aacttaaaac cttagaggct atttaagttg ctgatttata ttaattttat
     1441 tgttcaaaca tgagagctta gtacgtgaaa catgagagct tagtacgtta gccatgagag
     1501 cttagtacgt tagccatgag ggtttagttc gttaaacatg agagcttagt acgttaaaca
     1561 tgagagctta gtacgtgaaa catgagagct tagtacgtac tatcaacagg ttgaactgct
     1621 gatcttcaga tcctctacgc cggacgcatc gtggccggat cttgcggccg caaaaattaa
     1681 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     1741 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     1801 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     1861 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     1921 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     1981 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     2041 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     2101 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     2161 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     2221 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     2281 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     2341 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     2401 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     2461 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     2521 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     2581 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     2641 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     2701 acatttcccc gaaaagtgcc acctgcatcg atggcccccg atggtagtgt ggggtctccc
     2761 catgcgagag tagggaactg ccaggcatca aataaaatga aaggctcagt cgaaagactg
     2821 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc
     2881 gggagcggat ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc
     2941 ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg acggatggcc tttttgcgtg
     3001 gccagtgcca agcttgcatg c
//
