LOCUS       pOD_004                 4366 bp    DNA     circular UNA 23-AUG-2020
DEFINITION  synthetic circular DNA
ACCESSION   07/06/2020 Addgene #155361
KEYWORDS    pDEL, Gentamicin
SOURCE      synthetic DNA construct
  ORGANISM  .
COMMENT     Construction: the vector was build by amplification of pDEL vector (Tikh et al. 2016) without the kan cassette (primers: oOD_068 GGGTTCGAAATGACCG / oOD_069 GCTTGCTGTCCATAAAACC) and then assembled by HiFi to the Zeocin cassette from Roth collection (strain TT24691).

            Sequencing: This vector was amplified with two primer pairs (oJZ_260 CAGAACGTTCAGCACCAC / oJZ_261 AAGGACCTGGTCATTACC and oJZ_262 GCTACCACATCGTCTTTGC / oJZ_263 CGTGATAGTTTGCGACAGTG). These amplicons were sequenced using PacBio RSII sequencer. The reads were mapped and variant called on the in silico reference with the tool Reseq from SMRT portal.

FEATURES             Location/Qualifiers
     rep_origin      82..463
                     /standard_name="R6K origin of replication"
     terminator      617..650
                     /standard_name="Terminator BBa-1002"
     terminator      654..687
                     /standard_name="Terminator BBa-1004"
     misc_feature    696..713
                     /note="Geneious type: domain"
                     /note="Geneious type: misc_feature"
                     /standard_name="I-SceI binding site"
     promoter        738..856
                     /standard_name="pRHA promoter"
     CDS             880..1587
                     /standard_name="I-SceI CDS"
     primer_bind     1629..1649
                     /standard_name="UL1"
     promoter        1842..1868
     gene            1935..2468
                     /note="Geneious type: CDS"
                     /standard_name="Gentamicin 3'-acetyltransferase CDS"
     primer_bind     complement(2706..2726)
                     /standard_name="UR1"
     misc_feature    2789..2801
                     /note="CAP binding site"
                     /standard_name="CAP binding site"
     promoter        2821..2850
                     /standard_name="pLac promoter"
     CDS             2945..4366
                     /note="SacB"
                     /standard_name="SacB CDS"
ORIGIN      
        1 cgtttaaggg caccaataac tgcagcgcta gtacggttat ccacagaatc aggggataac
       61 gcaggaaaga acatgtgaga tcagcagttc aacctgttga tagtacgtac taagctctca
      121 tgtttcacgt actaagctct catgtttaac gtactaagct ctcatgttta acgaactaaa
      181 ccctcatggc taacgtacta agctctcatg gctaacgtac taagctctca tgtttcacgt
      241 actaagctct catgtttgaa caataaaatt aatataaatc agcaacttaa atagcctcta
      301 aggttttaag ttttataaga aaaaaaagaa tatataaggc ttttaaagct agcttttaag
      361 gtttaacggt tgtggacaac aagccaggga tgtaacgcac tgagaagccc ttagagcctc
      421 tcaaagcaat tttgagtgac acaggaacac ttaacggctg acatggcctt agaaggcgtt
      481 ttagaagccc taatggatgg agtcaaaaca aactagcgat ctgacgctca gtggaacgaa
      541 aactcacgtt aagggatttt ggtcattgta attctccatg gtaattcacg acggaggcca
      601 cctcatgtta gtcatgcgca aaaaaccccg cttcggcggg gttttttcgc gcccgccgaa
      661 aaccccgctt cggcggggtt ttgccgctca gctactaggg ataacagggt aatgaccagg
      721 tccttcagaa cgttcagcac cacaattcag caaattgtga acatcatcac gttcatcttt
      781 ccctggttgc caatggccca ttttcctgtc agtaacgaga aggtcgcgaa ttcaggcgct
      841 ttttagactg gtcgtagttt aactttaagg aggggatcta tgaagaacat caaaaagaat
      901 caagtaatca atttcggacc taattctaaa ttattaaaag aatataaatc acaattaatt
      961 gaattaaata ttgaacaatt tgaagcaggt attggtttaa ttttaggaga tgcttatatt
     1021 cgtagtcgtg atgaaggtaa actatattgt atgcaatttg agtggaaaaa taaggcatac
     1081 atggatcatg tatgtttatt atatgatcaa tgggtattat cacctcctca taaaaaagaa
     1141 agagttaatc atttaggtaa tttagtaatt acctggggag ctcaaacttt caaacatcaa
     1201 gcttttaata aattggctaa cttattcatt gtaaataaca aaaagcttat tcctaacaat
     1261 ttagttgaaa attacttgac acctataagt ttggcatact ggtttataga tgatggaggt
     1321 aaatgggatt acaataaaaa ctctcttaat aaaagtatcg tattaaatac acaaagtttt
     1381 actttcgaag aagtagaata tttggttaaa ggtttaagaa ataaattcca attgaattgt
     1441 tatgttaaaa tcaataaaaa caaaccaatc atctacattg attctataag ttatttaatc
     1501 ttttataatt tgatcaaacc ttacttaatc cctcaaatga tatacaaatt acctaatact
     1561 atctcatccg aaactttctt gaaataacat ggcgatagct agactgggcg gttttatgga
     1621 cagcaagcca ccaaacaccc cccaaaacca agccactgga gcacctcaaa aacaccatca
     1681 tacactaaat cagtaagttg gcagcaccac ccgacgcact ttgcgccgaa taaatacctg
     1741 tgacggaaga tcacttcgca gaataaataa atcctggtgt ccctgttgat accgggaagc
     1801 cctgggccaa cttttggcga aaatgagacg ttgatcggca cgtaagaggt tccaactttc
     1861 accataatga aataagatca ctaccgggcg tattttttga gttatcgaga ttttcaggag
     1921 ctaaggaagc taaaatgtta cgcagcagca acgatgttac gcagcagggc agtcgcccta
     1981 aaacaaagtt aggtggctca agtatgggca tcattcgcac atgtaggctc ggccctgacc
     2041 aagtcaaatc catgcgggct gctcttgatc ttttcggtcg tgagttcgga gacgtagcca
     2101 cctactccca acatcagccg gactccgatt acctcgggaa cttgctccgt agtaagacat
     2161 tcatcgcgct tgctgccttc gaccaagaag cggttgttgg cgctctcgcg gcttacgttc
     2221 tgcccaagtt tgagcagccg cgtagtgaga tctatatcta tgatctcgca gtctccggcg
     2281 agcaccggag gcagggcatt gccaccgcgc tcatcaatct cctcaagcat gaggccaacg
     2341 cgcttggtgc ttatgtgatc tacgtgcaag cagattacgg tgacgatccc gcagtggctc
     2401 tctatacaaa gttgggcata cgggaagaag tgatgcactt tgatatcgac ccaagtaccg
     2461 ccacctaatt tttttaaggc agttattggt gcccttaaac gcctggttgc tacgcctgaa
     2521 taagtgataa taagcggatg aatggcagaa attcgaaagc aaattcgacc cggtcgtcgg
     2581 ttcagggcag ggtcgttaaa tagccgctta tgtctattgc tggtttaccg gtttattgac
     2641 taccggaagc agtgtgaccg tgtgcttctc aaatgcctga ggccagtttg ctcaggctct
     2701 cccgtgtggt gtggtgtggt tgtgtggggt tcgaaatgac cgaccaagcg acatcataac
     2761 ggttctggca aatattctgg caattaatgt gagttagctc actcattagg caccccaggc
     2821 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     2881 cacaggaaac agccagtccg tttaggtgtt ttcacgagca attgaccaac aggaggatct
     2941 ggaaatgaac atcaaaaagt ttgcaaaaca agcaacagta ttaaccttta ctaccgcact
     3001 gctggcagga ggcgcaactc aagcgtttgc gaaagaaacg aaccaaaagc catataagga
     3061 aacatacggc atttcccata ttacacgcca tgatatgctg caaatccctg aacagcaaaa
     3121 aaatgaaaaa tatcaagttc ctgagttcga ttcgtccaca attaaaaata tctcttctgc
     3181 aaaaggcctg gacgtttggg acagctggcc attacaaaac gctgacggca ctgtcgcaaa
     3241 ctatcacggc taccacatcg tctttgcatt agccggagat cctaaaaatg cggatgacac
     3301 atcgatttac atgttctatc aaaaagtcgg cgaaacttct attgacagct ggaaaaacgc
     3361 tggccgcgtc tttaaagaca gcgacaaatt cgatgcaaat gattctatcc taaaagacca
     3421 aacacaagaa tggtcaggtt cagccacatt tacatctgac ggaaaaatcc gtttattcta
     3481 cactgatttc tccggtaaac attacggcaa acaaacactg acaactgcac aagttaacgt
     3541 atcagcatca gacagctctt tgaacatcaa cggtgtagag gattataaat caatctttga
     3601 cggtgacgga aaaacgtatc aaaatgtaca gcagttcatc gatgaaggca actacagctc
     3661 aggcgacaac catacgctga gagatcctca ctacgtagaa gataaaggcc acaaatactt
     3721 agtatttgaa gcaaacactg gaactgaaga tggctaccaa ggcgaagaat ctttatttaa
     3781 caaagcatac tatggcaaaa gcacatcatt cttccgtcaa gaaagtcaaa aacttctgca
     3841 aagcgataaa aaacgcacgg ctgagttagc aaacggcgct ctcggtatga ttgagctaaa
     3901 cgatgattac acactgaaaa aagtgatgaa accgctgatt gcatctaaca cagtaacaga
     3961 tgaaattgaa cgcgcgaacg tctttaaaat gaacggcaaa tggtatctgt tcactgactc
     4021 ccgcggatca aaaatgacga ttgacggcat tacgtctaac gatatttaca tgcttggtta
     4081 tgtttctaat tctttaactg gcccatacaa gccgctgaac aaaactggcc ttgtgttaaa
     4141 aatggatctt gatcctaacg atgtaacctt tacttactca cacttcgctg tacctcaagc
     4201 gaaaggaaac aatgtcgtga ttacaagcta tatgacaaac agaggattct acgcagacaa
     4261 acaatcaacg tttgcgccca gcttcctgct gaacatcaaa ggcaagaaaa catctgttgt
     4321 caaagacagc atccttgaac aaggacaatt aacagttaac aaataa
//
