LOCUS       pJK398_sgRNA_repressila 2691 bp ds-DNA     circular     19-NOV-2019
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     rep_origin      complement(1331..1876)
                     /label="p15A ori"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(join(2132..2691,1..301))
                     /label="AmpR"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     Terminator      705..765
                     /label="L3S2P21"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    981..1015
                     /label="P_A1"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    776..974
                     /label="PCR2 (from pAN-AND)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        complement(302..406)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    567..601
                     /label="P_A2"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     Terminator      1119..1175
                     /label="L3S2P55"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_RNA        622..697
                     /label="g RNA scaffold"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1016..1118
                     /label="A2NT"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    776..810
                     /label="P_A4"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    1197..1997
                     /label="p15A from pdCas9"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    557..769
                     /label="PCR1 (pAN-OR)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     terminator      496..539
                     /label="bacterial terminator"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_RNA        1036..1111
                     /label="g RNA scaffold"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1998..2037
                     /label="found in LPT41"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     64..83
                     /label="Amp-R"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    811..913
                     /label="sgRNA_A1NT"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    989..1001
                     /label="oper A1"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    914..970
                     /label="L3S2P11 (error in SI)"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     474..492
                     /label="pBRforEco"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer_bind     complement(1411..1430)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    602..704
                     /label="sgRNA_A4NT"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer_bind     complement(1197..1214)
                     /label="L4440"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    1016..1179
                     /label="PCR3 (from pAN-OR)"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    join(1998..2691,1..1196)
                     /label="from pJK388"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(1177..1211)
                     /label="398-388-R"
                     /note="sequence: gagtcagtgagcgaggaagcctgcagtctagataa"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          769..798
                     /label="386-BgH-F"
                     /note="sequence: agatctaagctttttacacctccacaactagctgg"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          1983..2017
                     /label="398-388-F"
                     /note="sequence: gttactagtgcttggcagtggaacgaaaactcacg"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(1978..2017)
                     /label="398-p15A-R"
                     /note="sequence: cgtgagttttcgttccactgccaagcactagtaacaactt"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          215..240
                     /label="mid-Amp-F"
                     /note="sequence: caccagcgtttctgggtgagcaaaaa"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          975..1035
                     /label="387-BB-PA1-F"
                     /note="sequence: agatctggatcctttacaccTAGCTCAGTCCTAggtattatgctagcataatacccgtgtgacccgt"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(953..980)
                     /label="386-Bam-R"
                     /note="sequence: ggatcctatcggaccaaaacgaaaaaag"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(1262..1281)
                     /label="433end-R"
                     /note="sequence: atcttccaggaaatctccgc"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(1154..1185)
                     /label="387-Xba-R"
                     /note="sequence: tctagataaaggaccaaaacgaaaaaagacgc"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          551..579
                     /label="385-BglII-F"
                     /note="sequence: agatctcagaaatcattttacaccaacgg"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          476..497
                     /label="AHC138-end-F"
                     /note="sequence: taggcgtatcacgaggcagaat"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          complement(747..779)
                     /label="385-HindIII-R"
                     /note="sequence: atataagcttaagcggaccaaaacgaaaaaagg"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          1184..1216
                     /label="398-p15A-F"
                     /note="sequence: gactgcaggcttcctcgctcactgactcgctac"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
ORIGIN
        1 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
       61 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
      121 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
      181 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
      241 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
      301 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
      361 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
      421 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
      481 gtatcacgag gcagaatttc agataaaaaa aatccttagc tttcgctaag gatgatttct
      541 ggaattctaa agatctcaga aatcatttta caccaacggg tcacacgggt attatgctag
      601 cataatacca gctagttgtg ggttttagag ctagaaatag caagttaaaa taaggctagt
      661 ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt ttttctcggt accaaattcc
      721 agaaaagagg cctcccgaaa ggggggcctt ttttcgtttt ggtccgctta agctttttac
      781 acctccacaa ctagctggta ttatgctagc ataataccta ggactgagct gttttagagc
      841 tagaaatagc aagttaaaat aaggctagtc cgattgcaac ttgaaaaagt ggcaccgagt
      901 cggtgctttt tttctcggta ccaaattcca gaaaagagac gctttcgagc gtcttttttc
      961 gttttggtcc gataggatcc tttacaccTA GCTCAGTCCT Aggtattatg ctagcataat
     1021 acccgtgtga cccgtgtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta
     1081 tcaacttgaa aaagtggcac cgagtcggtg ctttttttct cggtaccaaa gacgaacaat
     1141 aagacgctga aaagcgtctt ttttcgtttt ggtcctttat ctagactgca ggcttcctcg
     1201 ctcactgact cgctacgctc ggtcgttcga ctgcggcgag cggaaatggc ttacgaacgg
     1261 ggcggagatt tcctggaaga tgccaggaag atacttaaca gggaagtgag agggccgcgg
     1321 caaagccgtt tttccatagg ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa
     1381 atcagtggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggcggct
     1441 ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg ctgttatggc
     1501 cgcgtttgtc tcattccacg cctgacactc agttccgggt aggcagttcg ctccaagctg
     1561 gactgtatgc acgaaccccc cgttcagtcc gaccgctgcg ccttatccgg taactatcgt
     1621 cttgagtcca acccggaaag acatgcaaaa gcaccactgg cagcagccac tggtaattga
     1681 tttagaggag ttagtcttga agtcatgcgc cggttaaggc taaactgaaa ggacaagttt
     1741 tggtgactgc gctcctccaa gccagttacc tcggttcaaa gagttggtag ctcagagaac
     1801 cttcgaaaaa ccgccctgca aggcggtttt ttcgttttca gagcaagaga ttacgcgcag
     1861 accaaaacga tctcaagaag atcatcttat taatcagata aaatatttct agatttcagt
     1921 gcaatttatc tcttcaaatg tagcacctga agtcagcccc atacgatata agttgttaag
     1981 ttgttactag tgcttggcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     2041 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     2101 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     2161 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     2221 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     2281 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     2341 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     2401 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     2461 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     2521 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     2581 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     2641 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca a
//