LOCUS       pJK412_dCRv1_dCas9_PA4- 9863 bp ds-DNA     circular     11-MAY-2020
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     misc_feature    5708..7937
                     /label="SC101 ori (from LPT119)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    8090..8806
                     /label="Venus"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     terminator      8963..9057
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     Terminator      5227..5287
                     /label="L3S2P21"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    4990..4995
                     /label="SpeI/AvrII scar"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     rep_origin      complement(7224..7446)
                     /label="pSC101 ori"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    5124..5226
                     /label="sgRNA_A4NT"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    5538..5701
                     /label="PCR3 (from pAN-OR)"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     promoter        7989..8063
                     /label="RiboJ"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    5503..5537
                     /label="P_A1"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer_bind     8743..8763
                     /label="GFP-F"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    5298..5332
                     /label="P_A4"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    5702..5707
                     /label="XbaI/AvrII scar"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    5436..5492
                     /label="L3S2P11 (error in SI)"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     terminator      5018..5061
                     /label="bacterial terminator"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             complement(6226..7176)
                     /label="rep101 CDS"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_RNA        7991..8040
                     /label="sTRSV HHRz"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     Terminator      5641..5697
                     /label="L3S2P55"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    7954..7988
                     /label="P_A4"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    5298..5496
                     /label="PCR2 (from pAN-AND)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    7938..7943
                     /label="AvrII/SpeI scar"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     538..557
                     /label="Tet-R"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    5333..5435
                     /label="sgRNA_A1NT"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             complement(9078..9737)
                     /label="CmR"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    7962..7974
                     /label="A4 oper"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     terminator      8864..8950
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     RBS             8070..8081
                     /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    5538..5640
                     /label="A2NT"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     promoter        649..704
                     /label="tetR/tetA promoter s"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    5089..5123
                     /label="P_A2"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    5079..5291
                     /label="PCR1 (pAN-OR)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    5511..5523
                     /label="oper A1"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     promoter        complement(9738..9840)
                     /label="cat promoter"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     protein_bind    685..703
                     /label="tet operator"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer_bind     complement(941..952)
                     /label="Puro-F"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    4996..5702
                     /label="dCRv1 from JK398"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     RBS             722..733
                     /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_RNA        5558..5633
                     /label="g RNA scaffold"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     terminator      4870..4941
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             740..4846
                     /label="dCas9"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer_bind     4996..5014
                     /label="pBRforEco"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     terminator      4957..4984
                     /label="T7Te terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer_bind     9652..9671
                     /label="CAT-R"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    8951..8956
                     /label="SpeI/XbaI scar"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(8120..8148)
                     /label="GFP-R"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_RNA        5144..5219
                     /label="g RNA scaffold"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             complement(7..630)
                     /label="TetR"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(7917..7943)
                     /label="382-SC101-R"
                     /note="sequence: attggtgagaatccaagcactagtccaagcactagggacagtaag"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          4984..5017
                     /label="SpeI-398-F"
                     /note="sequence: ATATATactagtaataggcgtatcacgaggcaga"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          7424..7443
                     /label="119_ori-F"
                     /note="sequence: gatcccagccctgtgtataa"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(7424..7448)
                     /label="119_ori-Rv"
                     /note="sequence: gtgagttatacacagggctgggatc"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(9046..9090)
                     /label="378-t0-Rv"
                     /note="sequence: gggcggggcgtaatttgatatcgagctcgcttggactcctgttga"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          4844..4869
                     /label="384-Xho-101-F"
                     /note="sequence: ATATctcgagtaaggatctccaggcat"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          complement(8115..8135)
                     /label="VenusR"
                     /note="sequence: caagaattgggacaactccag"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(5727..5746)
                     /label="SC101-R"
                     /note="sequence: acaccagaacagcccgtttg"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          complement(5727..5746)
                     /label="119-genomicINT-Rv"
                     /note="sequence: acaccagaacagcccgtttg"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
ORIGIN
        1 gacgtcttaa gacccacttt cacatttaag ttgtttttct aatccgcata tgatcaattc
       61 aaggccgaat aagaaggctg gctctgcacc ttggtgatca aataattcga tagcttgtcg
      121 taataatggc ggcatactat cagtagtagg tgtttccctt tcttctttag cgacttgatg
      181 ctcttgatct tccaatacgc aacctaaagt aaaatgcccc acagcgctga gtgcatataa
      241 tgcattctct agtgaaaaac cttgttggca taaaaaggct aattgatttt cgagagtttc
      301 atactgtttt tctgtaggcc gtgtacctaa atgtactttt gctccatcgc gatgacttag
      361 taaagcacat ctaaaacttt tagcgttatt acgtaaaaaa tcttgccagc tttccccttc
      421 taaagggcaa aagtgagtat ggtgcctatc taacatctca atggctaagg cgtcgagcaa
      481 agcccgctta ttttttacat gccaatacaa tgtaggctgc tctacaccta gcttctgggc
      541 gagtttacgg gttgttaaac cttcgattcc gacctcatta agcagctcta atgcgctgtt
      601 aatcacttta cttttatcta atctagacat cattaattcc taatttttgt tgacactcta
      661 tcgttgatag agttatttta ccactcccta tcagtgatag agaaaagaat tcaaaagatc
      721 taaagaggag aaaggatcta tggataagaa atactcaata ggcttagcta tcggcacaaa
      781 tagcgtcgga tgggcggtga tcactgatga atataaggtt ccgtctaaaa agttcaaggt
      841 tctgggaaat acagaccgcc acagtatcaa aaaaaatctt ataggggctc ttttatttga
      901 cagtggagag acagcggaag cgactcgtct caaacggaca gctcgtagaa ggtatacacg
      961 tcggaagaat cgtatttgtt atctacagga gattttttca aatgagatgg cgaaagtaga
     1021 tgatagtttc tttcatcgac ttgaagagtc ttttttggtg gaagaagaca agaagcatga
     1081 acgtcatcct atttttggaa atatagtaga tgaagttgct tatcatgaga aatatccaac
     1141 tatctatcat ctgcgaaaaa aattggtaga ttctactgat aaagcggatt tgcgcttaat
     1201 ctatttggcc ttagcgcata tgattaagtt tcgtggtcat tttttgattg agggagattt
     1261 aaatcctgat aatagtgatg tggacaaact atttatccag ttggtacaaa cctacaatca
     1321 attatttgaa gaaaacccta ttaacgcaag tggagtagat gctaaagcga ttctttctgc
     1381 acgattgagt aaatcaagac gattagaaaa tctcattgct cagctccccg gtgagaagaa
     1441 aaatggctta tttgggaatc tcattgcttt gtcattgggt ttgaccccta attttaaatc
     1501 aaattttgat ttggcagaag atgctaaatt acagctttca aaagatactt acgatgatga
     1561 tttagataat ttattggcgc aaattggaga tcaatatgct gatttgtttt tggcagctaa
     1621 gaatttatca gatgctattt tactttcaga tatcctaaga gtaaatactg aaataactaa
     1681 ggctccccta tcagcttcaa tgattaaacg ctacgatgaa catcatcaag acttgactct
     1741 tttaaaagct ttagttcgac aacaacttcc agaaaagtat aaagaaatct tttttgatca
     1801 atcaaaaaac ggatatgcag gttatattga tgggggagct agccaagaag aattttataa
     1861 atttatcaaa ccaattttag aaaaaatgga tggtactgag gaattattgg tgaaactaaa
     1921 tcgtgaagat ttgctgcgca agcaacggac ctttgacaac ggctctattc cccatcaaat
     1981 tcacttgggt gagctgcatg ctattttgag aagacaagaa gacttttatc catttttaaa
     2041 agacaatcgt gagaagattg aaaaaatctt gacttttcga attccttatt atgttggtcc
     2101 attggcgcgt ggcaatagtc gttttgcatg gatgactcgg aagtctgaag aaacaattac
     2161 cccatggaat tttgaagaag ttgtcgataa aggtgcttca gctcaatcat ttattgaacg
     2221 catgacaaac tttgataaaa atcttccaaa tgaaaaagta ctaccaaaac atagtttgct
     2281 ttatgagtat tttacggttt ataacgaatt gacaaaggtc aaatatgtta ctgaaggaat
     2341 gcgaaaacca gcatttcttt caggtgaaca gaagaaagcc attgttgatt tactcttcaa
     2401 aacaaatcga aaagtaaccg ttaagcaatt aaaagaagat tatttcaaaa aaatagaatg
     2461 ttttgatagt gttgaaattt caggagttga agatagattt aatgcttcat taggtaccta
     2521 ccatgatttg ctaaaaatta ttaaagataa agattttttg gataatgaag aaaatgaaga
     2581 tatcttagag gatattgttt taacattgac cttatttgaa gatagggaga tgattgagga
     2641 aagacttaaa acatatgctc acctctttga tgataaggtg atgaaacagc ttaaacgtcg
     2701 ccgttatact ggttggggac gtttgtctcg aaaattgatt aatggtatta gggataagca
     2761 atctggcaaa acaatattag attttttgaa atcagatggt tttgccaatc gcaattttat
     2821 gcagctgatc catgatgata gtttgacatt taaagaagac attcaaaaag cacaagtgtc
     2881 tggacaaggc gatagtttac atgaacatat tgcaaattta gctggtagcc ctgctattaa
     2941 aaaaggtatt ttacagactg taaaagttgt tgatgaattg gtcaaagtaa tggggcggca
     3001 taagccagaa aatatcgtta ttgaaatggc acgtgaaaat cagacaactc aaaagggcca
     3061 gaaaaattcg cgagagcgta tgaaacgaat cgaagaaggt atcaaagaat taggaagtca
     3121 gattcttaaa gagcatcctg ttgaaaatac tcaattgcaa aatgaaaagc tctatctcta
     3181 ttatctccaa aatggaagag acatgtatgt ggaccaagaa ttagatatta atcgtttaag
     3241 tgattatgat gtcgatgcca ttgttccaca aagtttcctt aaagacgatt caatagacaa
     3301 taaggtctta acgcgttctg ataaaaatcg tggtaaatcg gataacgttc caagtgaaga
     3361 agtagtcaaa aagatgaaaa actattggag acaacttcta aacgccaagt taatcactca
     3421 acgtaagttt gataatttaa cgaaagctga acgtggaggt ttgagtgaac ttgataaagc
     3481 tggttttatc aaacgccaat tggttgaaac tcgccaaatc actaagcatg tggcacaaat
     3541 tttggatagt cgcatgaata ctaaatacga tgaaaatgat aaacttattc gagaggttaa
     3601 agtgattacc ttaaaatcta aattagtttc tgacttccga aaagatttcc aattctataa
     3661 agtacgtgag attaacaatt accatcatgc ccatgatgcg tatctaaatg ccgtcgttgg
     3721 aactgctttg attaagaaat atccaaaact tgaatcggag tttgtctatg gtgattataa
     3781 agtttatgat gttcgtaaaa tgattgctaa gtctgagcaa gaaataggca aagcaaccgc
     3841 aaaatatttc ttttactcta atatcatgaa cttcttcaaa acagaaatta cacttgcaaa
     3901 tggagagatt cgcaaacgcc ctctaatcga aactaatggg gaaactggag aaattgtctg
     3961 ggataaaggg cgagattttg ccacagtgcg caaagtattg tccatgcccc aagtcaatat
     4021 tgtcaagaaa acagaagtac agacaggcgg attctccaag gagtcaattt taccaaaaag
     4081 aaattcggac aagcttattg ctcgtaaaaa agactgggat ccaaaaaaat atggtggttt
     4141 tgatagtcca acggtagctt attcagtcct agtggttgct aaggtggaaa aagggaaatc
     4201 gaagaagtta aaatccgtta aagagttact agggatcaca attatggaaa gaagttcctt
     4261 tgaaaaaaat ccgattgact ttttagaagc taaaggatat aaggaagtta aaaaagactt
     4321 aatcattaaa ctacctaaat atagtctttt tgagttagaa aacggtcgta aacggatgct
     4381 ggctagtgcc ggagaattac aaaaaggaaa tgagctggct ctgccaagca aatatgtgaa
     4441 ttttttatat ttagctagtc attatgaaaa gttgaagggt agtccagaag ataacgaaca
     4501 aaaacaattg tttgtggagc agcataagca ttatttagat gagattattg agcaaatcag
     4561 tgaattttct aagcgtgtta ttttagcaga tgccaattta gataaagttc ttagtgcata
     4621 taacaaacat agagacaaac caatacgtga acaagcagaa aatattattc atttatttac
     4681 gttgacgaat cttggagctc ccgctgcttt taaatatttt gatacaacaa ttgatcgtaa
     4741 acgatatacg tctacaaaag aagttttaga tgccactctt atccatcaat ccatcactgg
     4801 tctttatgaa acacgcattg atttgagtca gctaggaggt gactaactcg agtaaggatc
     4861 tccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     4921 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
     4981 tgcgtttatc ctagtaatag gcgtatcacg aggcagaatt tcagataaaa aaaatcctta
     5041 gctttcgcta aggatgattt ctggaattct aaagatctca gaaatcattt tacaccAACG
     5101 GGTCACACGg gtattatgct agcataatac cagctagttg tgggttttag agctagaaat
     5161 agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct
     5221 ttttttctcg gtaccaaatt ccagaaaaga ggcctcccga aaggggggcc ttttttcgtt
     5281 ttggtccgct taagcttttt acaccTCCAC AACTAGCTgg tattatgcta gcataatacc
     5341 taggactgag ctgttttaga gctagaaata gcaagttaaa ataaggctag tccgattgca
     5401 acttgaaaaa gtggcaccga gtcggtgctt tttttctcgg taccaaattc cagaaaagag
     5461 acgctttcga gcgtcttttt tcgttttggt ccgataggat cctttacacc TAGCTCAGTC
     5521 CTAggtatta tgctagcata atacccgtgt gacccgtgtt ttagagctag aaatagcaag
     5581 ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt
     5641 ctcggtacca aagacgaaca ataagacgct gaaaagcgtc ttttttcgtt ttggtccttt
     5701 atctagggta cgggttttgc tgcccgcaaa cgggctgttc tggtgttgct agtttgttat
     5761 cagaatcgca gatccggctt cagccggttt gccggctgaa agcgctattt cttccagaat
     5821 tgccatgatt ttttccccac gggaggcgtc actggctccc gtgttgtcgg cagctttgat
     5881 tcgataagca gcatcgcctg tttcaggctg tctatgtgtg actgttgagc tgtaacaagt
     5941 tgtctcaggt gttcaatttc atgttctagt tgctttgttt tactggtttc acctgttcta
     6001 ttaggtgtta catgctgttc atctgttaca ttgtcgatct gttcatggtg aacagctttg
     6061 aatgcaccaa aaactcgtaa aagctctgat gtatctatct tttttacacc gttttcatct
     6121 gtgcatatgg acagttttcc ctttgatatg taacggtgaa cagttgttct acttttgttt
     6181 gttagtcttg atgcttcact gatagataca agagccataa gaacctcaga tccttccgta
     6241 tttagccagt atgttctcta gtgtggttcg ttgtttttgc gtgagccatg agaacgaacc
     6301 attgagatca tacttacttt gcatgtcact caaaaatttt gcctcaaaac tggtgagctg
     6361 aatttttgca gttaaagcat cgtgtagtgt ttttcttagt ccgttatgta ggtaggaatc
     6421 tgatgtaatg gttgttggta ttttgtcacc attcattttt atctggttgt tctcaagttc
     6481 ggttacgaga tccatttgtc tatctagttc aacttggaaa atcaacgtat cagtcgggcg
     6541 gcctcgctta tcaaccacca atttcatatt gctgtaagtg tttaaatctt tacttattgg
     6601 tttcaaaacc cattggttaa gccttttaaa ctcatggtag ttattttcaa gcattaacat
     6661 gaacttaaat tcatcaaggc taatctctat atttgccttg tgagttttct tttgtgttag
     6721 ttcttttaat aaccactcat aaatcctcat agagtatttg ttttcaaaag acttaacatg
     6781 ttccagatta tattttatga atttttttaa ctggaaaaga taaggcaata tctcttcact
     6841 aaaaactaat tctaattttt cgcttgagaa cttggcatag tttgtccact ggaaaatctc
     6901 aaagccttta accaaaggat tcctgatttc cacagttctc gtcatcagct ctctggttgc
     6961 tttagctaat acaccataag cattttccct actgatgttc atcatctgag cgtattggtt
     7021 ataagtgaac gataccgtcc gttctttcct tgtagggttt tcaatcgtgg ggttgagtag
     7081 tgccacacag cataaaatta gcttggtttc atgctccgtt aagtcatagc gactaatcgc
     7141 tagttcattt gctttgaaaa caactaattc agacatacat ctcaattggt ctaggtgatt
     7201 ttaatcacta taccaattga gatgggctag tcaatgataa ttactagtcc ttttcctttg
     7261 agttgtgggt atctgtaaat tctgctagac ctttgctgga aaacttgtaa attctgctag
     7321 accctctgta aattccgcta gacctttgtg tgtttttttt gtttatattc aagtggttat
     7381 aatttataga ataaagaaag aataaaaaaa gataaaaaga atagatccca gccctgtgta
     7441 taactcacta ctttagtcag ttccgcagta ttacaaaagg atgtcgcaaa cgctgtttgc
     7501 tcctctacaa aacagacctt aaaaccctaa aggcttaagt agcaccctcg caagctcggg
     7561 caaatcgctg aatattcctt ttgtctccga ccatcaggca cctgagtcgc tgtctttttc
     7621 gtgacattca gttcgctgcg ctcacggctc tggcagtgaa tgggggtaaa tggcactaca
     7681 ggcgcctttt atggattcat gcaaggaaac tacccataat acaagaaaag cccgtcacgg
     7741 gcttctcagg gcgttttatg gcgggtctgc tatgtggtgc tatctgactt tttgctgttc
     7801 agcagttcct gccctctgat tttccagtct gaccacttcg gattatcccg tgacaggtca
     7861 ttcagactgg ctaatgcacc cagtaaggca gcggtatcat caacaggctt acccgtctta
     7921 ctgtccctag tgcttggcct agtatgcgtc tcctttacac ctccacaact agctggtatt
     7981 atgctagcag ctgtcaccgg atgtgctttc cggtctgatg agtccgtgag gacgaaacag
     8041 cctctacaaa taattttgtt taactttaca aagaggagaa aggtaccgca tgagtaaagg
     8101 agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg
     8161 gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct
     8221 taaattgatt tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt
     8281 gggttatggt gttcaatgct ttgcgagata cccagatcat atgaaacagc atgacttttt
     8341 caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg
     8401 gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga
     8461 gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa
     8521 ctataactca cacaatgtat acatcacggc agacaaacaa aagaatggaa tcaaagcgaa
     8581 cttcaaaatt agacacaaca ttgaagatgg aggtgttcaa ctagcagacc attatcaaca
     8641 aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc tgtcctacca
     8701 atctaagctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt
     8761 aacagctgct gggattacac atggcatgga tgaactatac aaataagctt gatatcgaat
     8821 tcctgcagcc cgggggatcc cattgtacgc gtgctagagg catcaaataa aacgaaaggc
     8881 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag
     8941 taggacaaat actagagctt ggattctcac caataaaaaa cgcccggcgg caaccgagcg
     9001 ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa
     9061 gcgagctcga tatcaaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca
     9121 ttaagcattc tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc
     9181 ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag
     9241 aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccca gggattggct
     9301 gagacgaaaa acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa
     9361 cacgccacat cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc
     9421 cagagcgatg aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta
     9481 tcccatatca ccagctcacc gtctttcatt gccatacgaa attccggatg agcattcatc
     9541 aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc
     9601 tttaaaaagg ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac
     9661 tgaaatgcct caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca
     9721 gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat
     9781 acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgccgatca
     9841 acgtctcatt ttcgccagat atc
//