LOCUS       pJK422_dCR412_clontegr 12893 bp ds-DNA     circular     23-MAY-2019
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     terminator      6957..6984
                     /label="T7Te terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    7538..7701
                     /label="PCR3 (from pAN-OR)"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             8872..9882
                     /label="phage 186 integrase"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    7298..7496
                     /label="PCR2 (from pAN-AND)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     protein_bind    11452..11666
                     /label="phage 186 attP"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     RBS             8842..8864
                     /label="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     promoter        complement(1875..1977)
                     /label="cat promoter"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     protein_bind    11392..11439
                     /label="FRT"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     protein_bind    2685..2703
                     /label="tet operator"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     Terminator      7227..7287
                     /label="L3S2P21"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    227..943
                     /label="Venus"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     1789..1808
                     /label="CAT-R"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    11367..11384
                     /label="P2 (Cui 2017)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    6996..7702
                     /label="dCRv1 from JK398"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1088..1093
                     /label="SpeI/XbaI scar"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     RBS             207..218
                     /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_RNA        7144..7219
                     /label="g RNA scaffold"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     RBS             2722..2733
                     /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     protein_bind    7980..8013
                     /label="FRT (minimal)"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             complement(8020..8733)
                     /label="-lambda repressor (ts)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer_bind     12292..12310
                     /label="pBRforEco"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        126..200
                     /label="RiboJ"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    7722..7741
                     /label="BioBrick suffix"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    76..80
                     /label="AvrII/SpeI scar"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     Terminator      7641..7697
                     /label="L3S2P55"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    7503..7537
                     /label="P_A1"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     terminator      1001..1087
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer_bind     complement(10129..10148)
                     /label="Kan-R"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer_bind     complement(2941..2952)
                     /label="Puro-F"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    7702..7707
                     /label="XbaI/AvrII scar"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             complement(2007..2630)
                     /label="TetR"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    7089..7123
                     /label="P_A2"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     terminator      12351..12437
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_RNA        7558..7633
                     /label="g RNA scaffold"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    7079..7291
                     /label="PCR1 (pAN-OR)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(10045..10064)
                     /label="pENTR-R"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    7333..7435
                     /label="sgRNA_A1NT"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer_bind     6996..7014
                     /label="pBRforEco"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    7511..7523
                     /label="oper A1"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             complement(1215..1874)
                     /label="CmR"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     promoter        2649..2704
                     /label="tetR/tetA promoter s"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer_bind     complement(257..285)
                     /label="GFP-R"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    6990..6995
                     /label="SpeI/AvrII scar"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer_bind     complement(12119..12141)
                     /label="pGEX 3'"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     terminator      12717..12803
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     terminator      7796..7823
                     /label="rrnB T2 terminator"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    7538..7640
                     /label="A2NT"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     2538..2557
                     /label="Tet-R"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    76..7721
                     /label="from pJK412"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     terminator      11783..12029
                     /label="-lambda tL3 terminator"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     terminator      1100..1194
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     RBS             8822..8830
                     /label="Shine-Dalgarno sequence"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_RNA        128..177
                     /label="sTRSV HHRz"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     terminator      6870..6941
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer_bind     880..900
                     /label="GFP-F"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     terminator      7872..7903
                     /label="tonB terminator"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     terminator      7018..7061
                     /label="bacterial terminator"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    99..111
                     /label="A4 oper"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             2740..6846
                     /label="dCas9"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    7298..7332
                     /label="P_A4"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             10056..10871
                     /label="KanR"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    7124..7226
                     /label="sgRNA_A4NT"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     rep_origin      10993..11381
                     /label="R6K gamma ori"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(11829..11846)
                     /label="P3 (Cui 2017)"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     terminator      12534..12620
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    7436..7492
                     /label="L3S2P11 (error in SI)"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    91..125
                     /label="P_A4"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          6998..7019
                     /label="AHC138-end-F"
                     /note="sequence: taggcgtatcacgaggcagaat"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          complement(1172..1204)
                     /label="SpeI-lt0Vs-R"
                     /note="sequence: AATTACTAGTgactcctgttgatagatccagta"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          66..98
                     /label="Bss-412-F"
                     /note="sequence: TATAgcgcgcctagtatgcgtctcctttacacc"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          complement(8450..8476)
                     /label="lambda_cI-F"
                     /note="sequence: cgagatgtatgaagcggttagtatgca"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          complement(7702..7728)
                     /label="435-SpeI-R"
                     /note="sequence: AATTACTAGTagcaaaacccgtaccctaga"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          6936..6963
                     /label="Bss-425-412-F"
                     /note="sequence: TATAGCGCGCtactagagtcacactggctcac"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          7073..7101
                     /label="385-BglII-F"
                     /note="sequence: agatctcagaaatcattttacaccaacgg"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          complement(7701..7731)
                     /label="SpeI-412-R"
                     /note="sequence: AATTactagtagcaaaacccgtaccctagat"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(209..229)
                     /label="381_Venus-F"
                     /note="sequence: catgcggtacctttctcctct"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 gaattcgagc tcggtacccg gggatcctct agagggctta ctaaaagcca gataacagta
       61 tgcgtatttg cgcgcctagt atgcgtctcc tttacacctc cacaactagc tggtattatg
      121 ctagcagctg tcaccggatg tgctttccgg tctgatgagt ccgtgaggac gaaacagcct
      181 ctacaaataa ttttgtttaa ctttacaaag aggagaaagg taccgcatga gtaaaggaga
      241 agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca
      301 caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa
      361 attgatttgc actactggaa aactacctgt tccatggcca acacttgtca ctactttggg
      421 ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa
      481 gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa
      541 ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt
      601 aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta
      661 taactcacac aatgtataca tcacggcaga caaacaaaag aatggaatca aagcgaactt
      721 caaaattaga cacaacattg aagatggagg tgttcaacta gcagaccatt atcaacaaaa
      781 tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt cctaccaatc
      841 taagctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac
      901 agctgctggg attacacatg gcatggatga actatacaaa taagcttgat atcgaattcc
      961 tgcagcccgg gggatcccat tgtacgcgtg ctagaggcat caaataaaac gaaaggctca
     1021 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag
     1081 gacaaatact agagcttgga ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc
     1141 tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg
     1201 agctcgatat caaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta
     1261 agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc
     1321 atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag
     1381 ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag
     1441 acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac
     1501 gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag
     1561 agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc
     1621 catatcacca gctcaccgtc tttcattgcc atacgaaatt ccggatgagc attcatcagg
     1681 cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt
     1741 aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga
     1801 aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg
     1861 atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg
     1921 cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg ccgatcaacg
     1981 tctcattttc gccagatatc gacgtcttaa gacccacttt cacatttaag ttgtttttct
     2041 aatccgcata tgatcaattc aaggccgaat aagaaggctg gctctgcacc ttggtgatca
     2101 aataattcga tagcttgtcg taataatggc ggcatactat cagtagtagg tgtttccctt
     2161 tcttctttag cgacttgatg ctcttgatct tccaatacgc aacctaaagt aaaatgcccc
     2221 acagcgctga gtgcatataa tgcattctct agtgaaaaac cttgttggca taaaaaggct
     2281 aattgatttt cgagagtttc atactgtttt tctgtaggcc gtgtacctaa atgtactttt
     2341 gctccatcgc gatgacttag taaagcacat ctaaaacttt tagcgttatt acgtaaaaaa
     2401 tcttgccagc tttccccttc taaagggcaa aagtgagtat ggtgcctatc taacatctca
     2461 atggctaagg cgtcgagcaa agcccgctta ttttttacat gccaatacaa tgtaggctgc
     2521 tctacaccta gcttctgggc gagtttacgg gttgttaaac cttcgattcc gacctcatta
     2581 agcagctcta atgcgctgtt aatcacttta cttttatcta atctagacat cattaattcc
     2641 taatttttgt tgacactcta tcgttgatag agttatttta ccactcccta tcagtgatag
     2701 agaaaagaat tcaaaagatc taaagaggag aaaggatcta tggataagaa atactcaata
     2761 ggcttagcta tcggcacaaa tagcgtcgga tgggcggtga tcactgatga atataaggtt
     2821 ccgtctaaaa agttcaaggt tctgggaaat acagaccgcc acagtatcaa aaaaaatctt
     2881 ataggggctc ttttatttga cagtggagag acagcggaag cgactcgtct caaacggaca
     2941 gctcgtagaa ggtatacacg tcggaagaat cgtatttgtt atctacagga gattttttca
     3001 aatgagatgg cgaaagtaga tgatagtttc tttcatcgac ttgaagagtc ttttttggtg
     3061 gaagaagaca agaagcatga acgtcatcct atttttggaa atatagtaga tgaagttgct
     3121 tatcatgaga aatatccaac tatctatcat ctgcgaaaaa aattggtaga ttctactgat
     3181 aaagcggatt tgcgcttaat ctatttggcc ttagcgcata tgattaagtt tcgtggtcat
     3241 tttttgattg agggagattt aaatcctgat aatagtgatg tggacaaact atttatccag
     3301 ttggtacaaa cctacaatca attatttgaa gaaaacccta ttaacgcaag tggagtagat
     3361 gctaaagcga ttctttctgc acgattgagt aaatcaagac gattagaaaa tctcattgct
     3421 cagctccccg gtgagaagaa aaatggctta tttgggaatc tcattgcttt gtcattgggt
     3481 ttgaccccta attttaaatc aaattttgat ttggcagaag atgctaaatt acagctttca
     3541 aaagatactt acgatgatga tttagataat ttattggcgc aaattggaga tcaatatgct
     3601 gatttgtttt tggcagctaa gaatttatca gatgctattt tactttcaga tatcctaaga
     3661 gtaaatactg aaataactaa ggctccccta tcagcttcaa tgattaaacg ctacgatgaa
     3721 catcatcaag acttgactct tttaaaagct ttagttcgac aacaacttcc agaaaagtat
     3781 aaagaaatct tttttgatca atcaaaaaac ggatatgcag gttatattga tgggggagct
     3841 agccaagaag aattttataa atttatcaaa ccaattttag aaaaaatgga tggtactgag
     3901 gaattattgg tgaaactaaa tcgtgaagat ttgctgcgca agcaacggac ctttgacaac
     3961 ggctctattc cccatcaaat tcacttgggt gagctgcatg ctattttgag aagacaagaa
     4021 gacttttatc catttttaaa agacaatcgt gagaagattg aaaaaatctt gacttttcga
     4081 attccttatt atgttggtcc attggcgcgt ggcaatagtc gttttgcatg gatgactcgg
     4141 aagtctgaag aaacaattac cccatggaat tttgaagaag ttgtcgataa aggtgcttca
     4201 gctcaatcat ttattgaacg catgacaaac tttgataaaa atcttccaaa tgaaaaagta
     4261 ctaccaaaac atagtttgct ttatgagtat tttacggttt ataacgaatt gacaaaggtc
     4321 aaatatgtta ctgaaggaat gcgaaaacca gcatttcttt caggtgaaca gaagaaagcc
     4381 attgttgatt tactcttcaa aacaaatcga aaagtaaccg ttaagcaatt aaaagaagat
     4441 tatttcaaaa aaatagaatg ttttgatagt gttgaaattt caggagttga agatagattt
     4501 aatgcttcat taggtaccta ccatgatttg ctaaaaatta ttaaagataa agattttttg
     4561 gataatgaag aaaatgaaga tatcttagag gatattgttt taacattgac cttatttgaa
     4621 gatagggaga tgattgagga aagacttaaa acatatgctc acctctttga tgataaggtg
     4681 atgaaacagc ttaaacgtcg ccgttatact ggttggggac gtttgtctcg aaaattgatt
     4741 aatggtatta gggataagca atctggcaaa acaatattag attttttgaa atcagatggt
     4801 tttgccaatc gcaattttat gcagctgatc catgatgata gtttgacatt taaagaagac
     4861 attcaaaaag cacaagtgtc tggacaaggc gatagtttac atgaacatat tgcaaattta
     4921 gctggtagcc ctgctattaa aaaaggtatt ttacagactg taaaagttgt tgatgaattg
     4981 gtcaaagtaa tggggcggca taagccagaa aatatcgtta ttgaaatggc acgtgaaaat
     5041 cagacaactc aaaagggcca gaaaaattcg cgagagcgta tgaaacgaat cgaagaaggt
     5101 atcaaagaat taggaagtca gattcttaaa gagcatcctg ttgaaaatac tcaattgcaa
     5161 aatgaaaagc tctatctcta ttatctccaa aatggaagag acatgtatgt ggaccaagaa
     5221 ttagatatta atcgtttaag tgattatgat gtcgatgcca ttgttccaca aagtttcctt
     5281 aaagacgatt caatagacaa taaggtctta acgcgttctg ataaaaatcg tggtaaatcg
     5341 gataacgttc caagtgaaga agtagtcaaa aagatgaaaa actattggag acaacttcta
     5401 aacgccaagt taatcactca acgtaagttt gataatttaa cgaaagctga acgtggaggt
     5461 ttgagtgaac ttgataaagc tggttttatc aaacgccaat tggttgaaac tcgccaaatc
     5521 actaagcatg tggcacaaat tttggatagt cgcatgaata ctaaatacga tgaaaatgat
     5581 aaacttattc gagaggttaa agtgattacc ttaaaatcta aattagtttc tgacttccga
     5641 aaagatttcc aattctataa agtacgtgag attaacaatt accatcatgc ccatgatgcg
     5701 tatctaaatg ccgtcgttgg aactgctttg attaagaaat atccaaaact tgaatcggag
     5761 tttgtctatg gtgattataa agtttatgat gttcgtaaaa tgattgctaa gtctgagcaa
     5821 gaaataggca aagcaaccgc aaaatatttc ttttactcta atatcatgaa cttcttcaaa
     5881 acagaaatta cacttgcaaa tggagagatt cgcaaacgcc ctctaatcga aactaatggg
     5941 gaaactggag aaattgtctg ggataaaggg cgagattttg ccacagtgcg caaagtattg
     6001 tccatgcccc aagtcaatat tgtcaagaaa acagaagtac agacaggcgg attctccaag
     6061 gagtcaattt taccaaaaag aaattcggac aagcttattg ctcgtaaaaa agactgggat
     6121 ccaaaaaaat atggtggttt tgatagtcca acggtagctt attcagtcct agtggttgct
     6181 aaggtggaaa aagggaaatc gaagaagtta aaatccgtta aagagttact agggatcaca
     6241 attatggaaa gaagttcctt tgaaaaaaat ccgattgact ttttagaagc taaaggatat
     6301 aaggaagtta aaaaagactt aatcattaaa ctacctaaat atagtctttt tgagttagaa
     6361 aacggtcgta aacggatgct ggctagtgcc ggagaattac aaaaaggaaa tgagctggct
     6421 ctgccaagca aatatgtgaa ttttttatat ttagctagtc attatgaaaa gttgaagggt
     6481 agtccagaag ataacgaaca aaaacaattg tttgtggagc agcataagca ttatttagat
     6541 gagattattg agcaaatcag tgaattttct aagcgtgtta ttttagcaga tgccaattta
     6601 gataaagttc ttagtgcata taacaaacat agagacaaac caatacgtga acaagcagaa
     6661 aatattattc atttatttac gttgacgaat cttggagctc ccgctgcttt taaatatttt
     6721 gatacaacaa ttgatcgtaa acgatatacg tctacaaaag aagttttaga tgccactctt
     6781 atccatcaat ccatcactgg tctttatgaa acacgcattg atttgagtca gctaggaggt
     6841 gactaactcg agtaaggatc tccaggcatc aaataaaacg aaaggctcag tcgaaagact
     6901 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
     6961 caccttcggg tgggcctttc tgcgtttatc ctagtaatag gcgtatcacg aggcagaatt
     7021 tcagataaaa aaaatcctta gctttcgcta aggatgattt ctggaattct aaagatctca
     7081 gaaatcattt tacaccaacg ggtcacacgg gtattatgct agcataatac cagctagttg
     7141 tgggttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa
     7201 agtggcaccg agtcggtgct ttttttctcg gtaccaaatt ccagaaaaga ggcctcccga
     7261 aaggggggcc ttttttcgtt ttggtccgct taagcttttt acacctccac aactagctgg
     7321 tattatgcta gcataatacc taggactgag ctgttttaga gctagaaata gcaagttaaa
     7381 ataaggctag tccgattgca acttgaaaaa gtggcaccga gtcggtgctt tttttctcgg
     7441 taccaaattc cagaaaagag acgctttcga gcgtcttttt tcgttttggt ccgataggat
     7501 cctttacacc TAGCTCAGTC CTAggtatta tgctagcata atacccgtgt gacccgtgtt
     7561 ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc
     7621 accgagtcgg tgcttttttt ctcggtacca aagacgaaca ataagacgct gaaaagcgtc
     7681 ttttttcgtt ttggtccttt atctagggta cgggttttgc tactagtagc ggccgctgca
     7741 ggcatgcctc gagatgcatg gcgcctaacc taaactgaca ggcatcaaat taagcagaag
     7801 gccatcctga cggatggcct ttttgcgttt cgaacaattg aaaaaacctc gcgccttacc
     7861 tgttgagtaa tagtcaaaag cctccggtcg gaggcttttg actttctgct tactgaattt
     7921 cggtggtgcc gttaattaac cggtgggccc tcatgataat aatggtttct tagacgtccg
     7981 aagttcctat tctctagaaa gtataggaac ttccctaggt cagccaaacg tctcttcagg
     8041 ccactgacta gcgataactt tccccacaac ggaacaactc tcattgcatg ggatcattgg
     8101 gtactgtggg tttagtggtt gtaaaaacac ctgaccgcta tccctgatca gtttcttgaa
     8161 ggtaaactca tcacccccaa gtctggctat gcagaaatca cctggctcaa cagcctgctc
     8221 agggtcaacg agaattaaca ttccgtcagg aaagcttggc ttggagcctg ttggtgcggt
     8281 catggaatta ccttcaacct caagccagaa tgcagaatca ctggcttttt tggttgtgct
     8341 tacccatctc tccgcatcac ctttggtaaa ggttctaagc ttaggtgaga acatccctgc
     8401 ctgaacatga gaaaaaacag ggtactcata ctcacttcta agtgacggct gcatactaac
     8461 cgcttcatac atctcgtaga tttctctggc gattgaaggg ctaaattctt caacgctaac
     8521 tttgagaatt tttgtaagca atgcggcgtt ataagcattt aatgcattga tgccattaaa
     8581 taaagcacca acgcctgact gccccatccc catcttgtct gcgacagatt cctgggataa
     8641 gccaagttca tttttctttt tttcataaat tgctttaagg cgacgtgcgt cctcaagctg
     8701 ctcttgtgtt aatggtttct tttttgtgct catacgttaa atctatcacc gcaagggata
     8761 aatatctaac accgtgcgtg ttgactattt tacctctggc ggtgataatg gttgcatgta
     8821 ctaaggaggt tgtatacgcg ttttgtttaa ctttaagaag gagatataca tatgaccgtc
     8881 cgtaaaaatc cggctggcgg ttggatttgt gaactctacc caaacggtgc aaaaggcaaa
     8941 cgtatcagaa agaaattcgc tactaaaggc gaggctctgg cgtttgagca gtacaccgtt
     9001 caaaacccgt ggcaggaaga aaaggaagac aggcgcacgt taaaagagct ggttgattca
     9061 tggtatagcg ctcatggcat tacactgaaa gatggtttga aacgccagtt agccatgcac
     9121 catgcttttg agtgtatggg cgaaccactc gcacgcgatt tcgatgcgca gatgttttcc
     9181 cgctaccgag aaaaacggtt aaaaggtgag tatgcccgtt caaacagagt gaaagaggta
     9241 tcgcctcgca cgcttaatct tgagctggcc tacttccggg cggtgttcaa tgagctaaac
     9301 cgcctcggag aatggaaggg tgaaaaccca ctaaaaaata tgcgcccatt ccgcacagaa
     9361 gaaatggaaa tgacttggct aactcacgac caaatttcgc aactgctcgg agagtgtaac
     9421 cgacatgacc accctgattt agaaaccgtg gtaagaatct gtctcgcaac tggcgcacgg
     9481 tggtctgagg ccgagagtct gagaaaaagc caactcgcga aatacaaaat cacatacacc
     9541 aacacgaaag gtagaaaaaa tcgcaccgtc ccaatcagca aagagctcta tgagtctctg
     9601 cctgatgata aaaagggccg gttgttcagt gattgttatg gcgcgttccg gtcagctttg
     9661 gaaagaacag gtatcgaact accggcagga caacttaccc acgttttacg tcacaccttc
     9721 gctagtcact ttatgatgaa tggcggtaat attttggtgt tgcagcgcgt actcggccat
     9781 acagatataa aaatgaccat gcgatatgcg cactttgcac ccgaccattt agaggatgca
     9841 gttaaactaa atcctttagt tcacattact aacagcaaat aagttatgga ggttgtatgt
     9901 cagttcggct agcccatggg tatggacagt tttccctttg atatgtaacg cacgttgtgt
     9961 ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac
    10021 tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt
    10081 cttgctcccg tccgcgctta aactccaaca tggacgctga tttatatggg tataaatggg
    10141 ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg
    10201 cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga
    10261 tggtccgtct caactggctg acggagttta tgcctctccc gaccatcaag cattttatcc
    10321 gtactcctga tgatgcgtgg ttactcacca ccgcgattcc tgggaaaaca gccttccagg
    10381 tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggcc gtgttcctgc
    10441 gccggttaca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgt gtatttcgtc
    10501 ttgctcaggc gcaatcacgc atgaataacg gtttggttga tgcgagtgat tttgatgacg
    10561 agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcacaagctc ttgccattct
    10621 caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg
    10681 ggaaattaat aggttgtatt gatgttggac gggtcggaat cgcagaccgt taccaggacc
    10741 ttgccattct ttggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc
    10801 aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg
    10861 agtttttcta ataatactag cagaaatcat ccttagcgaa agctaaggat tttttttatc
    10921 tgattaccgc ctttgagtga gcgtcgacct agtgcggccg caagatccgg ccacgatgcg
    10981 tccggcgtag aggatctgaa gatcagcagt tcaacctgtt gatagtacgt actaagctct
    11041 catgtttcac gtactaagct ctcatgttta acgtactaag ctctcatgtt taacgaacta
    11101 aaccctcatg gctaacgtac taagctctca tggctaacgt actaagctct catgtttcac
    11161 gtactaagct ctcatgtttg aacaataaaa ttaatataaa tcagcaactt aaatagcctc
    11221 taaggtttta agttttataa gaaaaaaaag aatatataag gcttttaaag cttttaaggt
    11281 ttaacggttg tggacaacaa gccagggatg taacgcactg agaagccctt agagcctctc
    11341 aaagcaattt tgagtgacac aggaacactt aacggctgac atgggaatta ggaagttcct
    11401 attccgaagt tcctattctc tagaaagtat aggaacttcc atatgccatg ggcgatggtt
    11461 ctgagtaaca gataatagaa tggcgataaa gtggcggtag aaatggcgaa taataggtaa
    11521 ttattggcaa acaatggcaa tctatgtcaa tgataaataa cgcatactat tgattttcgg
    11581 ttgttcttgt aggaactcat aatcgcttgg tcgctggttc aagtccagca ggggccacca
    11641 aatttaagta gtaaatacat acagttaggc cactctctcg agtggccttt ttgttgcctc
    11701 gttttcgagt ggcgatagaa tggcggtgga ttttttaccg ccactttcct gaggagtgca
    11761 tagctagctt cttcgtctgt ttctactggt attggcacaa acctgattcc aatttgagca
    11821 aggctatgtg ccatctcgat actcgttctt aactcaacag aagatgcttt gtgcatacag
    11881 cccctcgttt attatttatc tcctcagcca gccgctgtgc tttcagtgga tttcggataa
    11941 cagaaaggcc gggaaatacc cagcctcgct ttgtaacgga gtagagacga aagtgattgc
    12001 gcctacccgg atattatcgt gaggatgcgt catcgccatt aattcactga tcagtgatag
    12061 ctgtcaaaca tgagaattga tccggctgcc tcgcgcgttt cggtgatgac ggtgaaaacc
    12121 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
    12181 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc
    12241 agtcacgtag cgatagcgga gtgtatgctg cacatgacat taacctataa aaataggcgt
    12301 atcacgaggc cctttcgtct tcaagaatta attcccaatt ccccaggcat caaataaaac
    12361 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
    12421 tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag
    12481 ggtggcgggc aggacgcccg ccataaactg ccaggaatta attccccagg catcaaataa
    12541 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
    12601 ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag caacggcccg
    12661 gagggtggcg ggcaggacgc ccgccataaa ctgccaggaa ttaattcccc aggcatcaaa
    12721 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
    12781 acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgcg aagcaacggc
    12841 ccggagggtg gcgggcagga cgcccgccat aaactgccag gaattgggga tcg
//