LOCUS       pJK423_dCR413_clontegr 12874 bp ds-DNA     circular     05-JUL-2018
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     RBS             8823..8845
                     /label="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     RBS             207..218
                     /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     rep_origin      10974..11362
                     /label="R6K gamma ori"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        126..200
                     /label="RiboJ"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     terminator      6957..6984
                     /label="T7Te terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             complement(1215..1874)
                     /label="CmR"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    91..125
                     /label="P_A4"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(7202..7400)
                     /label="PCR2 (from pAN-AND)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     terminator      complement(7637..7680)
                     /label="bacterial terminator"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     protein_bind    7961..7994
                     /label="FRT (minimal)"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    99..111
                     /label="A4 oper"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     terminator      11764..12010
                     /label="-lambda tL3 terminator"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     terminator      6870..6941
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer_bind     880..900
                     /label="GFP-F"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    complement(7263..7365)
                     /label="sgRNA_A1NT"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer_bind     1789..1808
                     /label="CAT-R"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(7058..7160)
                     /label="A2NT"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     2538..2557
                     /label="Tet-R"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             complement(2007..2630)
                     /label="TetR"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer_bind     complement(10110..10129)
                     /label="Kan-R"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    complement(6996..7702)
                     /label="dCRv1 from JK398"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     promoter        complement(1875..1977)
                     /label="cat promoter"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    1088..1093
                     /label="SpeI/XbaI scar"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_RNA        complement(7065..7140)
                     /label="g RNA scaffold"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             8853..9863
                     /label="phage 186 integrase"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     terminator      1001..1087
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer_bind     complement(257..285)
                     /label="GFP-R"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer_bind     complement(2941..2952)
                     /label="Puro-F"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(7366..7400)
                     /label="P_A4"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     terminator      7777..7804
                     /label="rrnB T2 terminator"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    227..943
                     /label="Venus"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     complement(12100..12122)
                     /label="pGEX 3'"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    complement(7175..7187)
                     /label="oper A1"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             10037..10852
                     /label="KanR"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    complement(7472..7574)
                     /label="sgRNA_A4NT"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    6990..6995
                     /label="SpeI/AvrII scar"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     RBS             8803..8811
                     /label="Shine-Dalgarno sequence"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     promoter        2649..2704
                     /label="tetR/tetA promoter s"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     RBS             2722..2733
                     /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     complement(10026..10045)
                     /label="pENTR-R"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     terminator      7853..7884
                     /label="tonB terminator"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_RNA        complement(7479..7554)
                     /label="g RNA scaffold"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    76..7702
                     /label="from pJK413"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    complement(7407..7619)
                     /label="PCR1 (pAN-OR)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     terminator      12515..12601
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(7575..7609)
                     /label="P_A2"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             complement(8001..8714)
                     /label="-lambda repressor (ts)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    7703..7722
                     /label="BioBrick suffix"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(7206..7262)
                     /label="L3S2P11 (error in SI)"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(6997..7160)
                     /label="PCR3 (from pAN-OR)"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     protein_bind    11373..11420
                     /label="FRT"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     Terminator      complement(7411..7471)
                     /label="L3S2P21"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     terminator      1100..1194
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     terminator      12698..12784
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(7161..7195)
                     /label="P_A1"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     protein_bind    11433..11647
                     /label="phage 186 attP"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer_bind     complement(7684..7702)
                     /label="pBRforEco"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer_bind     12273..12291
                     /label="pBRforEco"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             2740..6846
                     /label="dCas9"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     protein_bind    2685..2703
                     /label="tet operator"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     Terminator      complement(7001..7057)
                     /label="L3S2P55"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_RNA        128..177
                     /label="sTRSV HHRz"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     terminator      12332..12418
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          6934..6961
                     /label="Bss-425-412-F"
                     /note="sequence: TATAGCGCGCtactagagtcacactggctcac"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          68..98
                     /label="Bss-412-F"
                     /note="sequence: TATAgcgcgcctagtatgcgtctcctttacacc"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          complement(7683..7712)
                     /label="SpeI-413-Rv"
                     /note="sequence: AATTactagtaataggcgtatcacgaggca"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
ORIGIN
        1 gaattcgagc tcggtacccg gggatcctct agagggctta ctaaaagcca gataacagta
       61 tgcgtatttg cgcgcctagt atgcgtctcc tttacacctc cacaactagc tggtattatg
      121 ctagcagctg tcaccggatg tgctttccgg tctgatgagt ccgtgaggac gaaacagcct
      181 ctacaaataa ttttgtttaa ctttacaaag aggagaaagg taccgcatga gtaaaggaga
      241 agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca
      301 caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa
      361 attgatttgc actactggaa aactacctgt tccatggcca acacttgtca ctactttggg
      421 ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa
      481 gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa
      541 ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt
      601 aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta
      661 taactcacac aatgtataca tcacggcaga caaacaaaag aatggaatca aagcgaactt
      721 caaaattaga cacaacattg aagatggagg tgttcaacta gcagaccatt atcaacaaaa
      781 tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt cctaccaatc
      841 taagctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac
      901 agctgctggg attacacatg gcatggatga actatacaaa taagcttgat atcgaattcc
      961 tgcagcccgg gggatcccat tgtacgcgtg ctagaggcat caaataaaac gaaaggctca
     1021 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag
     1081 gacaaatact agagcttgga ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc
     1141 tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg
     1201 agctcgatat caaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta
     1261 agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc
     1321 atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag
     1381 ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag
     1441 acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac
     1501 gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag
     1561 agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc
     1621 catatcacca gctcaccgtc tttcattgcc atacgaaatt ccggatgagc attcatcagg
     1681 cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt
     1741 aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga
     1801 aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg
     1861 atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg
     1921 cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg ccgatcaacg
     1981 tctcattttc gccagatatc gacgtcttaa gacccacttt cacatttaag ttgtttttct
     2041 aatccgcata tgatcaattc aaggccgaat aagaaggctg gctctgcacc ttggtgatca
     2101 aataattcga tagcttgtcg taataatggc ggcatactat cagtagtagg tgtttccctt
     2161 tcttctttag cgacttgatg ctcttgatct tccaatacgc aacctaaagt aaaatgcccc
     2221 acagcgctga gtgcatataa tgcattctct agtgaaaaac cttgttggca taaaaaggct
     2281 aattgatttt cgagagtttc atactgtttt tctgtaggcc gtgtacctaa atgtactttt
     2341 gctccatcgc gatgacttag taaagcacat ctaaaacttt tagcgttatt acgtaaaaaa
     2401 tcttgccagc tttccccttc taaagggcaa aagtgagtat ggtgcctatc taacatctca
     2461 atggctaagg cgtcgagcaa agcccgctta ttttttacat gccaatacaa tgtaggctgc
     2521 tctacaccta gcttctgggc gagtttacgg gttgttaaac cttcgattcc gacctcatta
     2581 agcagctcta atgcgctgtt aatcacttta cttttatcta atctagacat cattaattcc
     2641 taatttttgt tgacactcta tcgttgatag agttatttta ccactcccta tcagtgatag
     2701 agaaaagaat tcaaaagatc taaagaggag aaaggatcta tggataagaa atactcaata
     2761 ggcttagcta tcggcacaaa tagcgtcgga tgggcggtga tcactgatga atataaggtt
     2821 ccgtctaaaa agttcaaggt tctgggaaat acagaccgcc acagtatcaa aaaaaatctt
     2881 ataggggctc ttttatttga cagtggagag acagcggaag cgactcgtct caaacggaca
     2941 gctcgtagaa ggtatacacg tcggaagaat cgtatttgtt atctacagga gattttttca
     3001 aatgagatgg cgaaagtaga tgatagtttc tttcatcgac ttgaagagtc ttttttggtg
     3061 gaagaagaca agaagcatga acgtcatcct atttttggaa atatagtaga tgaagttgct
     3121 tatcatgaga aatatccaac tatctatcat ctgcgaaaaa aattggtaga ttctactgat
     3181 aaagcggatt tgcgcttaat ctatttggcc ttagcgcata tgattaagtt tcgtggtcat
     3241 tttttgattg agggagattt aaatcctgat aatagtgatg tggacaaact atttatccag
     3301 ttggtacaaa cctacaatca attatttgaa gaaaacccta ttaacgcaag tggagtagat
     3361 gctaaagcga ttctttctgc acgattgagt aaatcaagac gattagaaaa tctcattgct
     3421 cagctccccg gtgagaagaa aaatggctta tttgggaatc tcattgcttt gtcattgggt
     3481 ttgaccccta attttaaatc aaattttgat ttggcagaag atgctaaatt acagctttca
     3541 aaagatactt acgatgatga tttagataat ttattggcgc aaattggaga tcaatatgct
     3601 gatttgtttt tggcagctaa gaatttatca gatgctattt tactttcaga tatcctaaga
     3661 gtaaatactg aaataactaa ggctccccta tcagcttcaa tgattaaacg ctacgatgaa
     3721 catcatcaag acttgactct tttaaaagct ttagttcgac aacaacttcc agaaaagtat
     3781 aaagaaatct tttttgatca atcaaaaaac ggatatgcag gttatattga tgggggagct
     3841 agccaagaag aattttataa atttatcaaa ccaattttag aaaaaatgga tggtactgag
     3901 gaattattgg tgaaactaaa tcgtgaagat ttgctgcgca agcaacggac ctttgacaac
     3961 ggctctattc cccatcaaat tcacttgggt gagctgcatg ctattttgag aagacaagaa
     4021 gacttttatc catttttaaa agacaatcgt gagaagattg aaaaaatctt gacttttcga
     4081 attccttatt atgttggtcc attggcgcgt ggcaatagtc gttttgcatg gatgactcgg
     4141 aagtctgaag aaacaattac cccatggaat tttgaagaag ttgtcgataa aggtgcttca
     4201 gctcaatcat ttattgaacg catgacaaac tttgataaaa atcttccaaa tgaaaaagta
     4261 ctaccaaaac atagtttgct ttatgagtat tttacggttt ataacgaatt gacaaaggtc
     4321 aaatatgtta ctgaaggaat gcgaaaacca gcatttcttt caggtgaaca gaagaaagcc
     4381 attgttgatt tactcttcaa aacaaatcga aaagtaaccg ttaagcaatt aaaagaagat
     4441 tatttcaaaa aaatagaatg ttttgatagt gttgaaattt caggagttga agatagattt
     4501 aatgcttcat taggtaccta ccatgatttg ctaaaaatta ttaaagataa agattttttg
     4561 gataatgaag aaaatgaaga tatcttagag gatattgttt taacattgac cttatttgaa
     4621 gatagggaga tgattgagga aagacttaaa acatatgctc acctctttga tgataaggtg
     4681 atgaaacagc ttaaacgtcg ccgttatact ggttggggac gtttgtctcg aaaattgatt
     4741 aatggtatta gggataagca atctggcaaa acaatattag attttttgaa atcagatggt
     4801 tttgccaatc gcaattttat gcagctgatc catgatgata gtttgacatt taaagaagac
     4861 attcaaaaag cacaagtgtc tggacaaggc gatagtttac atgaacatat tgcaaattta
     4921 gctggtagcc ctgctattaa aaaaggtatt ttacagactg taaaagttgt tgatgaattg
     4981 gtcaaagtaa tggggcggca taagccagaa aatatcgtta ttgaaatggc acgtgaaaat
     5041 cagacaactc aaaagggcca gaaaaattcg cgagagcgta tgaaacgaat cgaagaaggt
     5101 atcaaagaat taggaagtca gattcttaaa gagcatcctg ttgaaaatac tcaattgcaa
     5161 aatgaaaagc tctatctcta ttatctccaa aatggaagag acatgtatgt ggaccaagaa
     5221 ttagatatta atcgtttaag tgattatgat gtcgatgcca ttgttccaca aagtttcctt
     5281 aaagacgatt caatagacaa taaggtctta acgcgttctg ataaaaatcg tggtaaatcg
     5341 gataacgttc caagtgaaga agtagtcaaa aagatgaaaa actattggag acaacttcta
     5401 aacgccaagt taatcactca acgtaagttt gataatttaa cgaaagctga acgtggaggt
     5461 ttgagtgaac ttgataaagc tggttttatc aaacgccaat tggttgaaac tcgccaaatc
     5521 actaagcatg tggcacaaat tttggatagt cgcatgaata ctaaatacga tgaaaatgat
     5581 aaacttattc gagaggttaa agtgattacc ttaaaatcta aattagtttc tgacttccga
     5641 aaagatttcc aattctataa agtacgtgag attaacaatt accatcatgc ccatgatgcg
     5701 tatctaaatg ccgtcgttgg aactgctttg attaagaaat atccaaaact tgaatcggag
     5761 tttgtctatg gtgattataa agtttatgat gttcgtaaaa tgattgctaa gtctgagcaa
     5821 gaaataggca aagcaaccgc aaaatatttc ttttactcta atatcatgaa cttcttcaaa
     5881 acagaaatta cacttgcaaa tggagagatt cgcaaacgcc ctctaatcga aactaatggg
     5941 gaaactggag aaattgtctg ggataaaggg cgagattttg ccacagtgcg caaagtattg
     6001 tccatgcccc aagtcaatat tgtcaagaaa acagaagtac agacaggcgg attctccaag
     6061 gagtcaattt taccaaaaag aaattcggac aagcttattg ctcgtaaaaa agactgggat
     6121 ccaaaaaaat atggtggttt tgatagtcca acggtagctt attcagtcct agtggttgct
     6181 aaggtggaaa aagggaaatc gaagaagtta aaatccgtta aagagttact agggatcaca
     6241 attatggaaa gaagttcctt tgaaaaaaat ccgattgact ttttagaagc taaaggatat
     6301 aaggaagtta aaaaagactt aatcattaaa ctacctaaat atagtctttt tgagttagaa
     6361 aacggtcgta aacggatgct ggctagtgcc ggagaattac aaaaaggaaa tgagctggct
     6421 ctgccaagca aatatgtgaa ttttttatat ttagctagtc attatgaaaa gttgaagggt
     6481 agtccagaag ataacgaaca aaaacaattg tttgtggagc agcataagca ttatttagat
     6541 gagattattg agcaaatcag tgaattttct aagcgtgtta ttttagcaga tgccaattta
     6601 gataaagttc ttagtgcata taacaaacat agagacaaac caatacgtga acaagcagaa
     6661 aatattattc atttatttac gttgacgaat cttggagctc ccgctgcttt taaatatttt
     6721 gatacaacaa ttgatcgtaa acgatatacg tctacaaaag aagttttaga tgccactctt
     6781 atccatcaat ccatcactgg tctttatgaa acacgcattg atttgagtca gctaggaggt
     6841 gactaactcg agtaaggatc tccaggcatc aaataaaacg aaaggctcag tcgaaagact
     6901 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
     6961 caccttcggg tgggcctttc tgcgtttatc ctagtataaa ggaccaaaac gaaaaaagac
     7021 gcttttcagc gtcttattgt tcgtctttgg taccgagaaa aaaagcaccg actcggtgcc
     7081 actttttcaa gttgataacg gactagcctt attttaactt gctatttcta gctctaaaac
     7141 acgggtcaca cgggtattat gctagcataa taccTAGGAC TGAGCTAggt gtaaaggatc
     7201 ctatcggacc aaaacgaaaa aagacgctcg aaagcgtctc ttttctggaa tttggtaccg
     7261 agaaaaaaag caccgactcg gtgccacttt ttcaagttgc aatcggacta gccttatttt
     7321 aacttgctat ttctagctct aaaacagctc agtcctaggt attatgctag cataatacca
     7381 gctagttgtg gaggtgtaaa aagcttaagc ggaccaaaac gaaaaaaggc ccccctttcg
     7441 ggaggcctct tttctggaat ttggtaccga gaaaaaaagc accgactcgg tgccactttt
     7501 tcaagttgat aacggactag ccttatttta acttgctatt tctagctcta aaacccacaa
     7561 ctagctggta ttatgctagc ataatacccg tgtgacccgt tggtgtaaaa tgatttctga
     7621 gatctttaga attccagaaa tcatccttag cgaaagctaa ggattttttt tatctgaaat
     7681 tctgcctcgt gatacgccta ttactagtag cggccgctgc aggcatgcct cgagatgcat
     7741 ggcgcctaac ctaaactgac aggcatcaaa ttaagcagaa ggccatcctg acggatggcc
     7801 tttttgcgtt tcgaacaatt gaaaaaacct cgcgccttac ctgttgagta atagtcaaaa
     7861 gcctccggtc ggaggctttt gactttctgc ttactgaatt tcggtggtgc cgttaattaa
     7921 ccggtgggcc ctcatgataa taatggtttc ttagacgtcc gaagttccta ttctctagaa
     7981 agtataggaa cttccctagg tcagccaaac gtctcttcag gccactgact agcgataact
     8041 ttccccacaa cggaacaact ctcattgcat gggatcattg ggtactgtgg gtttagtggt
     8101 tgtaaaaaca cctgaccgct atccctgatc agtttcttga aggtaaactc atcaccccca
     8161 agtctggcta tgcagaaatc acctggctca acagcctgct cagggtcaac gagaattaac
     8221 attccgtcag gaaagcttgg cttggagcct gttggtgcgg tcatggaatt accttcaacc
     8281 tcaagccaga atgcagaatc actggctttt ttggttgtgc ttacccatct ctccgcatca
     8341 cctttggtaa aggttctaag cttaggtgag aacatccctg cctgaacatg agaaaaaaca
     8401 gggtactcat actcacttct aagtgacggc tgcatactaa ccgcttcata catctcgtag
     8461 atttctctgg cgattgaagg gctaaattct tcaacgctaa ctttgagaat ttttgtaagc
     8521 aatgcggcgt tataagcatt taatgcattg atgccattaa ataaagcacc aacgcctgac
     8581 tgccccatcc ccatcttgtc tgcgacagat tcctgggata agccaagttc atttttcttt
     8641 ttttcataaa ttgctttaag gcgacgtgcg tcctcaagct gctcttgtgt taatggtttc
     8701 ttttttgtgc tcatacgtta aatctatcac cgcaagggat aaatatctaa caccgtgcgt
     8761 gttgactatt ttacctctgg cggtgataat ggttgcatgt actaaggagg ttgtatacgc
     8821 gttttgttta actttaagaa ggagatatac atatgaccgt ccgtaaaaat ccggctggcg
     8881 gttggatttg tgaactctac ccaaacggtg caaaaggcaa acgtatcaga aagaaattcg
     8941 ctactaaagg cgaggctctg gcgtttgagc agtacaccgt tcaaaacccg tggcaggaag
     9001 aaaaggaaga caggcgcacg ttaaaagagc tggttgattc atggtatagc gctcatggca
     9061 ttacactgaa agatggtttg aaacgccagt tagccatgca ccatgctttt gagtgtatgg
     9121 gcgaaccact cgcacgcgat ttcgatgcgc agatgttttc ccgctaccga gaaaaacggt
     9181 taaaaggtga gtatgcccgt tcaaacagag tgaaagaggt atcgcctcgc acgcttaatc
     9241 ttgagctggc ctacttccgg gcggtgttca atgagctaaa ccgcctcgga gaatggaagg
     9301 gtgaaaaccc actaaaaaat atgcgcccat tccgcacaga agaaatggaa atgacttggc
     9361 taactcacga ccaaatttcg caactgctcg gagagtgtaa ccgacatgac caccctgatt
     9421 tagaaaccgt ggtaagaatc tgtctcgcaa ctggcgcacg gtggtctgag gccgagagtc
     9481 tgagaaaaag ccaactcgcg aaatacaaaa tcacatacac caacacgaaa ggtagaaaaa
     9541 atcgcaccgt cccaatcagc aaagagctct atgagtctct gcctgatgat aaaaagggcc
     9601 ggttgttcag tgattgttat ggcgcgttcc ggtcagcttt ggaaagaaca ggtatcgaac
     9661 taccggcagg acaacttacc cacgttttac gtcacacctt cgctagtcac tttatgatga
     9721 atggcggtaa tattttggtg ttgcagcgcg tactcggcca tacagatata aaaatgacca
     9781 tgcgatatgc gcactttgca cccgaccatt tagaggatgc agttaaacta aatcctttag
     9841 ttcacattac taacagcaaa taagttatgg aggttgtatg tcagttcggc tagcccatgg
     9901 gtatggacag ttttcccttt gatatgtaac gcacgttgtg tctcaaaatc tctgatgtta
     9961 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag
    10021 taatacaagg ggtgttatga gccatattca acgggaaacg tcttgctccc gtccgcgctt
    10081 aaactccaac atggacgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca
    10141 atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa
    10201 acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtccgtc tcaactggct
    10261 gacggagttt atgcctctcc cgaccatcaa gcattttatc cgtactcctg atgatgcgtg
    10321 gttactcacc accgcgattc ctgggaaaac agccttccag gtattagaag aatatcctga
    10381 ttcaggtgaa aatattgttg atgcgctggc cgtgttcctg cgccggttac attcgattcc
    10441 tgtttgtaat tgtcctttta acagcgatcg tgtatttcgt cttgctcagg cgcaatcacg
    10501 catgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt
    10561 tgaacaagtc tggaaagaaa tgcacaagct cttgccattc tcaccggatt cagtcgtcac
    10621 tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat
    10681 tgatgttgga cgggtcggaa tcgcagaccg ttaccaggac cttgccattc tttggaactg
    10741 cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa
    10801 tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct aataatacta
    10861 gcagaaatca tccttagcga aagctaagga ttttttttat ctgattaccg cctttgagtg
    10921 agcgtcgacc tagtgcggcc gcaagatccg gccacgatgc gtccggcgta gaggatctga
    10981 agatcagcag ttcaacctgt tgatagtacg tactaagctc tcatgtttca cgtactaagc
    11041 tctcatgttt aacgtactaa gctctcatgt ttaacgaact aaaccctcat ggctaacgta
    11101 ctaagctctc atggctaacg tactaagctc tcatgtttca cgtactaagc tctcatgttt
    11161 gaacaataaa attaatataa atcagcaact taaatagcct ctaaggtttt aagttttata
    11221 agaaaaaaaa gaatatataa ggcttttaaa gcttttaagg tttaacggtt gtggacaaca
    11281 agccagggat gtaacgcact gagaagccct tagagcctct caaagcaatt ttgagtgaca
    11341 caggaacact taacggctga catgggaatt aggaagttcc tattccgaag ttcctattct
    11401 ctagaaagta taggaacttc catatgccat gggcgatggt tctgagtaac agataataga
    11461 atggcgataa agtggcggta gaaatggcga ataataggta attattggca aacaatggca
    11521 atctatgtca atgataaata acgcatacta ttgattttcg gttgttcttg taggaactca
    11581 taatcgcttg gtcgctggtt caagtccagc aggggccacc aaatttaagt agtaaataca
    11641 tacagttagg ccactctctc gagtggcctt tttgttgcct cgttttcgag tggcgataga
    11701 atggcggtgg attttttacc gccactttcc tgaggagtgc atagctagct tcttcgtctg
    11761 tttctactgg tattggcaca aacctgattc caatttgagc aaggctatgt gccatctcga
    11821 tactcgttct taactcaaca gaagatgctt tgtgcataca gcccctcgtt tattatttat
    11881 ctcctcagcc agccgctgtg ctttcagtgg atttcggata acagaaaggc cgggaaatac
    11941 ccagcctcgc tttgtaacgg agtagagacg aaagtgattg cgcctacccg gatattatcg
    12001 tgaggatgcg tcatcgccat taattcactg atcagtgata gctgtcaaac atgagaattg
    12061 atccggctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
    12121 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
    12181 gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg
    12241 agtgtatgct gcacatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
    12301 ttcaagaatt aattcccaat tccccaggca tcaaataaaa cgaaaggctc agtcgaaaga
    12361 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc
    12421 gccgggagcg gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc
    12481 gccataaact gccaggaatt aattccccag gcatcaaata aaacgaaagg ctcagtcgaa
    12541 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa
    12601 tccgccggga gcggatttga acgttgcgaa gcaacggccc ggagggtggc gggcaggacg
    12661 cccgccataa actgccagga attaattccc caggcatcaa ataaaacgaa aggctcagtc
    12721 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac
    12781 aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg
    12841 acgcccgcca taaactgcca ggaattgggg atcg
//