LOCUS       pJK430v2_PphlF-A2NT_dC 10815 bp ds-DNA     circular     23-JUN-2020
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     misc_feature    5079..5291
                     /label="PCR1 (pAN-OR)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    5506..5540
                     /label="PJ23101"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    5089..5123
                     /label="P_A2"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_RNA        8943..8992
                     /label="sTRSV HHRz"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    5333..5435
                     /label="sgRNA_A1NT"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer_bind     complement(9072..9100)
                     /label="GFP-R"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    5436..5492
                     /label="L3S2P11 (error in SI)"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     rep_origin      complement(8176..8398)
                     /label="pSC101 ori"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     terminator      9816..9902
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer_bind     complement(941..952)
                     /label="Puro-F"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    4996..6653
                     /label="from pJK411"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     insulator       5545..5624
                     /label="LtsvJ"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     terminator      4957..4984
                     /label="T7Te terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             740..4846
                     /label="dCas9"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4990..4995
                     /label="SpeI/AvrII scar"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    6409..6474
                     /label="PphlF"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     promoter        complement(10690..10792)
                     /label="cat promoter"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_RNA        6495..6570
                     /label="g RNA scaffold"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Terminator      6578..6634
                     /label="L3S2P55"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     terminator      6371..6398
                     /label="T7Te terminator"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     terminator      6284..6355
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    6660..8889
                     /label="SC101 ori (from LPT119)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     Terminator      5227..5287
                     /label="L3S2P21"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     terminator      5018..5061
                     /label="bacterial terminator"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     promoter        649..704
                     /label="tetR/tetA promoter s"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     terminator      4870..4941
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    5658..6260
                     /label="PhlF"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6654..6654
                     /label="dCRv1 from JK398"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    5298..5496
                     /label="PCR2 (from pAN-AND)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     538..557
                     /label="Tet-R"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     terminator      9915..10009
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    8906..8940
                     /label="P_A4"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    6475..6577
                     /label="A2NT"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    8890..8895
                     /label="AvrII/SpeI scar"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_RNA        5144..5219
                     /label="g RNA scaffold"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    5298..5332
                     /label="P_A4"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    6276..6355
                     /label="B0010 rrnB terminator"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             complement(10030..10689)
                     /label="CmR"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             complement(7..630)
                     /label="TetR"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             complement(7178..8128)
                     /label="rep101 CDS"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     RBS             9022..9033
                     /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     promoter        8941..9015
                     /label="RiboJ"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     4996..5014
                     /label="pBRforEco"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    9042..9758
                     /label="Venus"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    5124..5226
                     /label="sgRNA_A4NT"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    8914..8926
                     /label="A4 oper"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    9903..9908
                     /label="SpeI/XbaI scar"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     protein_bind    685..703
                     /label="tet operator"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     RBS             722..733
                     /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     9695..9715
                     /label="GFP-F"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    6654..6659
                     /label="XbaI/AvrII scar"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer_bind     10604..10623
                     /label="CAT-R"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          complement(6679..6698)
                     /label="119-genomicINT-Rv"
                     /note="sequence: acaccagaacagcccgtttg"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(5475..5502)
                     /label="386-Bam-R"
                     /note="sequence: ggatcctatcggaccaaaacgaaaaaag"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(5269..5301)
                     /label="385-HindIII-R"
                     /note="sequence: atataagcttaagcggaccaaaacgaaaaaagg"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          8376..8395
                     /label="119_ori-F"
                     /note="sequence: gatcccagccctgtgtataa"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(6654..6683)
                     /label="435-SpeI-R"
                     /note="sequence: AATTACTAGTagcaaaacccgtaccctaga"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          5493..5524
                     /label="411-Bam-PhlF-F"
                     /note="sequence: TTAAggatccacttttacagctagctcagtcc"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          complement(8376..8400)
                     /label="119_ori-Rv"
                     /note="sequence: gtgagttatacacagggctgggatc"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(6679..6698)
                     /label="SC101-R"
                     /note="sequence: acaccagaacagcccgtttg"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          4998..5019
                     /label="AHC138-end-F"
                     /note="sequence: taggcgtatcacgaggcagaat"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          complement(9998..10042)
                     /label="378-t0-Rv"
                     /note="sequence: gggcggggcgtaatttgatatcgagctcgcttggactcctgttga"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          4843..4869
                     /label="434-Psp-F"
                     /note="sequence: ctaactcgagtaaggatctccaggcat"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(9067..9087)
                     /label="VenusR"
                     /note="sequence: caagaattgggacaactccag"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(6833..6849)
                     /label="SC101R2"
                     /note="sequence: gcgatgctgcttatcga"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          8889..8913
                     /label="Bss-412-F"
                     /note="sequence: TATAgcgcgcctagtatgcgtctcctttacacc"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          complement(6634..6663)
                     /label="430-XbaI-R2"
                     /note="sequence: TTAATCTAGAagcctgcaggaattctaaag"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          5291..5320
                     /label="386-BgH-F"
                     /note="sequence: agatctaagctttttacacctccacaactagctgg"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(8869..8895)
                     /label="382-SC101-R"
                     /note="sequence: attggtgagaatccaagcactagtccaagcactagggacagtaag"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          5073..5101
                     /label="385-BglII-F"
                     /note="sequence: agatctcagaaatcattttacaccaacgg"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          4844..4869
                     /label="384-Xho-101-F"
                     /note="sequence: ATATctcgagtaaggatctccaggcat"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
ORIGIN
        1 gacgtcttaa gacccacttt cacatttaag ttgtttttct aatccgcata tgatcaattc
       61 aaggccgaat aagaaggctg gctctgcacc ttggtgatca aataattcga tagcttgtcg
      121 taataatggc ggcatactat cagtagtagg tgtttccctt tcttctttag cgacttgatg
      181 ctcttgatct tccaatacgc aacctaaagt aaaatgcccc acagcgctga gtgcatataa
      241 tgcattctct agtgaaaaac cttgttggca taaaaaggct aattgatttt cgagagtttc
      301 atactgtttt tctgtaggcc gtgtacctaa atgtactttt gctccatcgc gatgacttag
      361 taaagcacat ctaaaacttt tagcgttatt acgtaaaaaa tcttgccagc tttccccttc
      421 taaagggcaa aagtgagtat ggtgcctatc taacatctca atggctaagg cgtcgagcaa
      481 agcccgctta ttttttacat gccaatacaa tgtaggctgc tctacaccta gcttctgggc
      541 gagtttacgg gttgttaaac cttcgattcc gacctcatta agcagctcta atgcgctgtt
      601 aatcacttta cttttatcta atctagacat cattaattcc taatttttgt tgacactcta
      661 tcgttgatag agttatttta ccactcccta tcagtgatag agaaaagaat tcaaaagatc
      721 taaagaggag aaaggatcta tggataagaa atactcaata ggcttagcta tcggcacaaa
      781 tagcgtcgga tgggcggtga tcactgatga atataaggtt ccgtctaaaa agttcaaggt
      841 tctgggaaat acagaccgcc acagtatcaa aaaaaatctt ataggggctc ttttatttga
      901 cagtggagag acagcggaag cgactcgtct caaacggaca gctcgtagaa ggtatacacg
      961 tcggaagaat cgtatttgtt atctacagga gattttttca aatgagatgg cgaaagtaga
     1021 tgatagtttc tttcatcgac ttgaagagtc ttttttggtg gaagaagaca agaagcatga
     1081 acgtcatcct atttttggaa atatagtaga tgaagttgct tatcatgaga aatatccaac
     1141 tatctatcat ctgcgaaaaa aattggtaga ttctactgat aaagcggatt tgcgcttaat
     1201 ctatttggcc ttagcgcata tgattaagtt tcgtggtcat tttttgattg agggagattt
     1261 aaatcctgat aatagtgatg tggacaaact atttatccag ttggtacaaa cctacaatca
     1321 attatttgaa gaaaacccta ttaacgcaag tggagtagat gctaaagcga ttctttctgc
     1381 acgattgagt aaatcaagac gattagaaaa tctcattgct cagctccccg gtgagaagaa
     1441 aaatggctta tttgggaatc tcattgcttt gtcattgggt ttgaccccta attttaaatc
     1501 aaattttgat ttggcagaag atgctaaatt acagctttca aaagatactt acgatgatga
     1561 tttagataat ttattggcgc aaattggaga tcaatatgct gatttgtttt tggcagctaa
     1621 gaatttatca gatgctattt tactttcaga tatcctaaga gtaaatactg aaataactaa
     1681 ggctccccta tcagcttcaa tgattaaacg ctacgatgaa catcatcaag acttgactct
     1741 tttaaaagct ttagttcgac aacaacttcc agaaaagtat aaagaaatct tttttgatca
     1801 atcaaaaaac ggatatgcag gttatattga tgggggagct agccaagaag aattttataa
     1861 atttatcaaa ccaattttag aaaaaatgga tggtactgag gaattattgg tgaaactaaa
     1921 tcgtgaagat ttgctgcgca agcaacggac ctttgacaac ggctctattc cccatcaaat
     1981 tcacttgggt gagctgcatg ctattttgag aagacaagaa gacttttatc catttttaaa
     2041 agacaatcgt gagaagattg aaaaaatctt gacttttcga attccttatt atgttggtcc
     2101 attggcgcgt ggcaatagtc gttttgcatg gatgactcgg aagtctgaag aaacaattac
     2161 cccatggaat tttgaagaag ttgtcgataa aggtgcttca gctcaatcat ttattgaacg
     2221 catgacaaac tttgataaaa atcttccaaa tgaaaaagta ctaccaaaac atagtttgct
     2281 ttatgagtat tttacggttt ataacgaatt gacaaaggtc aaatatgtta ctgaaggaat
     2341 gcgaaaacca gcatttcttt caggtgaaca gaagaaagcc attgttgatt tactcttcaa
     2401 aacaaatcga aaagtaaccg ttaagcaatt aaaagaagat tatttcaaaa aaatagaatg
     2461 ttttgatagt gttgaaattt caggagttga agatagattt aatgcttcat taggtaccta
     2521 ccatgatttg ctaaaaatta ttaaagataa agattttttg gataatgaag aaaatgaaga
     2581 tatcttagag gatattgttt taacattgac cttatttgaa gatagggaga tgattgagga
     2641 aagacttaaa acatatgctc acctctttga tgataaggtg atgaaacagc ttaaacgtcg
     2701 ccgttatact ggttggggac gtttgtctcg aaaattgatt aatggtatta gggataagca
     2761 atctggcaaa acaatattag attttttgaa atcagatggt tttgccaatc gcaattttat
     2821 gcagctgatc catgatgata gtttgacatt taaagaagac attcaaaaag cacaagtgtc
     2881 tggacaaggc gatagtttac atgaacatat tgcaaattta gctggtagcc ctgctattaa
     2941 aaaaggtatt ttacagactg taaaagttgt tgatgaattg gtcaaagtaa tggggcggca
     3001 taagccagaa aatatcgtta ttgaaatggc acgtgaaaat cagacaactc aaaagggcca
     3061 gaaaaattcg cgagagcgta tgaaacgaat cgaagaaggt atcaaagaat taggaagtca
     3121 gattcttaaa gagcatcctg ttgaaaatac tcaattgcaa aatgaaaagc tctatctcta
     3181 ttatctccaa aatggaagag acatgtatgt ggaccaagaa ttagatatta atcgtttaag
     3241 tgattatgat gtcgatgcca ttgttccaca aagtttcctt aaagacgatt caatagacaa
     3301 taaggtctta acgcgttctg ataaaaatcg tggtaaatcg gataacgttc caagtgaaga
     3361 agtagtcaaa aagatgaaaa actattggag acaacttcta aacgccaagt taatcactca
     3421 acgtaagttt gataatttaa cgaaagctga acgtggaggt ttgagtgaac ttgataaagc
     3481 tggttttatc aaacgccaat tggttgaaac tcgccaaatc actaagcatg tggcacaaat
     3541 tttggatagt cgcatgaata ctaaatacga tgaaaatgat aaacttattc gagaggttaa
     3601 agtgattacc ttaaaatcta aattagtttc tgacttccga aaagatttcc aattctataa
     3661 agtacgtgag attaacaatt accatcatgc ccatgatgcg tatctaaatg ccgtcgttgg
     3721 aactgctttg attaagaaat atccaaaact tgaatcggag tttgtctatg gtgattataa
     3781 agtttatgat gttcgtaaaa tgattgctaa gtctgagcaa gaaataggca aagcaaccgc
     3841 aaaatatttc ttttactcta atatcatgaa cttcttcaaa acagaaatta cacttgcaaa
     3901 tggagagatt cgcaaacgcc ctctaatcga aactaatggg gaaactggag aaattgtctg
     3961 ggataaaggg cgagattttg ccacagtgcg caaagtattg tccatgcccc aagtcaatat
     4021 tgtcaagaaa acagaagtac agacaggcgg attctccaag gagtcaattt taccaaaaag
     4081 aaattcggac aagcttattg ctcgtaaaaa agactgggat ccaaaaaaat atggtggttt
     4141 tgatagtcca acggtagctt attcagtcct agtggttgct aaggtggaaa aagggaaatc
     4201 gaagaagtta aaatccgtta aagagttact agggatcaca attatggaaa gaagttcctt
     4261 tgaaaaaaat ccgattgact ttttagaagc taaaggatat aaggaagtta aaaaagactt
     4321 aatcattaaa ctacctaaat atagtctttt tgagttagaa aacggtcgta aacggatgct
     4381 ggctagtgcc ggagaattac aaaaaggaaa tgagctggct ctgccaagca aatatgtgaa
     4441 ttttttatat ttagctagtc attatgaaaa gttgaagggt agtccagaag ataacgaaca
     4501 aaaacaattg tttgtggagc agcataagca ttatttagat gagattattg agcaaatcag
     4561 tgaattttct aagcgtgtta ttttagcaga tgccaattta gataaagttc ttagtgcata
     4621 taacaaacat agagacaaac caatacgtga acaagcagaa aatattattc atttatttac
     4681 gttgacgaat cttggagctc ccgctgcttt taaatatttt gatacaacaa ttgatcgtaa
     4741 acgatatacg tctacaaaag aagttttaga tgccactctt atccatcaat ccatcactgg
     4801 tctttatgaa acacgcattg atttgagtca gctaggaggt gactaactcg agtaaggatc
     4861 tccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     4921 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
     4981 tgcgtttatc ctagtaatag gcgtatcacg aggcagaatt tcagataaaa aaaatcctta
     5041 gctttcgcta aggatgattt ctggaattct aaagatctca gaaatcattt tacaccaacg
     5101 ggtcacacgg gtattatgct agcataatac cagctagttg tgggttttag agctagaaat
     5161 agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct
     5221 ttttttctcg gtaccaaatt ccagaaaaga ggcctcccga aaggggggcc ttttttcgtt
     5281 ttggtccgct taagcttttt acacctccac aactagctgg tattatgcta gcataatacc
     5341 taggactgag ctgttttaga gctagaaata gcaagttaaa ataaggctag tccgattgca
     5401 acttgaaaaa gtggcaccga gtcggtgctt tttttctcgg taccaaattc cagaaaagag
     5461 acgctttcga gcgtcttttt tcgttttggt ccgataggat ccacttttac agctagctca
     5521 gtcctaggta ttatgctagc ctgaagtacg tctgagcgtg atacccgctc actgaagatg
     5581 gcccggtagg gccgaaacgt acctctacaa ataattttgt ttaactatgg actatgtttg
     5641 aaagggagaa atactagatg gcacgtaccc cgagccgtag cagcattggt agcctgcgta
     5701 gtccgcatac ccataaagca attctgacca gcaccattga aatcctgaaa gaatgtggtt
     5761 atagcggtct gagcattgaa agcgttgcac gtcgtgccgg tgcaagcaaa ccgaccattt
     5821 atcgttggtg gaccaataaa gcagcactga ttgccgaagt gtatgaaaat gaaagcgaac
     5881 aggtgcgtaa atttccggat ctgggtagct ttaaagccga tctggatttt ctgctgcgta
     5941 atctgtggaa agtttggcgt gaaaccattt gtggtgaagc atttcgttgt gttattgcag
     6001 aagcacagct ggaccctgca accctgaccc agctgaaaga tcagtttatg gaacgtcgtc
     6061 gtgagatgcc gaaaaaactg gttgaaaatg ccattagcaa tggtgaactg ccgaaagata
     6121 ccaatcgtga actgctgctg gatatgattt ttggtttttg ttggtatcgc ctgctgaccg
     6181 aacagctgac cgttgaacag gatattgaag aatttacctt cctgctgatt aatggtgttt
     6241 gtccgggtac acagcgttaa ctagggccca tacccccagg catcaaataa aacgaaaggc
     6301 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
     6361 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatacgcg acgtacggtg
     6421 gaatctgatt cgttaccaat tgacatgata cgaaacgtac cgtatcgtta aggtataata
     6481 cccgtgtgac ccgtgtttta gagctagaaa tagcaagtta aaataaggct agtccgttat
     6541 caacttgaaa aagtggcacc gagtcggtgc tttttttctc ggtaccaaag acgaacaata
     6601 agacgctgaa aagcgtcttt tttcgttttg gtcctttaga attcctgcag gcttctaggg
     6661 tacgggtttt gctgcccgca aacgggctgt tctggtgttg ctagtttgtt atcagaatcg
     6721 cagatccggc ttcagccggt ttgccggctg aaagcgctat ttcttccaga attgccatga
     6781 ttttttcccc acgggaggcg tcactggctc ccgtgttgtc ggcagctttg attcgataag
     6841 cagcatcgcc tgtttcaggc tgtctatgtg tgactgttga gctgtaacaa gttgtctcag
     6901 gtgttcaatt tcatgttcta gttgctttgt tttactggtt tcacctgttc tattaggtgt
     6961 tacatgctgt tcatctgtta cattgtcgat ctgttcatgg tgaacagctt tgaatgcacc
     7021 aaaaactcgt aaaagctctg atgtatctat cttttttaca ccgttttcat ctgtgcatat
     7081 ggacagtttt ccctttgata tgtaacggtg aacagttgtt ctacttttgt ttgttagtct
     7141 tgatgcttca ctgatagata caagagccat aagaacctca gatccttccg tatttagcca
     7201 gtatgttctc tagtgtggtt cgttgttttt gcgtgagcca tgagaacgaa ccattgagat
     7261 catacttact ttgcatgtca ctcaaaaatt ttgcctcaaa actggtgagc tgaatttttg
     7321 cagttaaagc atcgtgtagt gtttttctta gtccgttatg taggtaggaa tctgatgtaa
     7381 tggttgttgg tattttgtca ccattcattt ttatctggtt gttctcaagt tcggttacga
     7441 gatccatttg tctatctagt tcaacttgga aaatcaacgt atcagtcggg cggcctcgct
     7501 tatcaaccac caatttcata ttgctgtaag tgtttaaatc tttacttatt ggtttcaaaa
     7561 cccattggtt aagcctttta aactcatggt agttattttc aagcattaac atgaacttaa
     7621 attcatcaag gctaatctct atatttgcct tgtgagtttt cttttgtgtt agttctttta
     7681 ataaccactc ataaatcctc atagagtatt tgttttcaaa agacttaaca tgttccagat
     7741 tatattttat gaattttttt aactggaaaa gataaggcaa tatctcttca ctaaaaacta
     7801 attctaattt ttcgcttgag aacttggcat agtttgtcca ctggaaaatc tcaaagcctt
     7861 taaccaaagg attcctgatt tccacagttc tcgtcatcag ctctctggtt gctttagcta
     7921 atacaccata agcattttcc ctactgatgt tcatcatctg agcgtattgg ttataagtga
     7981 acgataccgt ccgttctttc cttgtagggt tttcaatcgt ggggttgagt agtgccacac
     8041 agcataaaat tagcttggtt tcatgctccg ttaagtcata gcgactaatc gctagttcat
     8101 ttgctttgaa aacaactaat tcagacatac atctcaattg gtctaggtga ttttaatcac
     8161 tataccaatt gagatgggct agtcaatgat aattactagt ccttttcctt tgagttgtgg
     8221 gtatctgtaa attctgctag acctttgctg gaaaacttgt aaattctgct agaccctctg
     8281 taaattccgc tagacctttg tgtgtttttt ttgtttatat tcaagtggtt ataatttata
     8341 gaataaagaa agaataaaaa aagataaaaa gaatagatcc cagccctgtg tataactcac
     8401 tactttagtc agttccgcag tattacaaaa ggatgtcgca aacgctgttt gctcctctac
     8461 aaaacagacc ttaaaaccct aaaggcttaa gtagcaccct cgcaagctcg ggcaaatcgc
     8521 tgaatattcc ttttgtctcc gaccatcagg cacctgagtc gctgtctttt tcgtgacatt
     8581 cagttcgctg cgctcacggc tctggcagtg aatgggggta aatggcacta caggcgcctt
     8641 ttatggattc atgcaaggaa actacccata atacaagaaa agcccgtcac gggcttctca
     8701 gggcgtttta tggcgggtct gctatgtggt gctatctgac tttttgctgt tcagcagttc
     8761 ctgccctctg attttccagt ctgaccactt cggattatcc cgtgacaggt cattcagact
     8821 ggctaatgca cccagtaagg cagcggtatc atcaacaggc ttacccgtct tactgtccct
     8881 agtgcttggc ctagtatgcg tctcctttac acctccacaa ctagctggta ttatgctagc
     8941 agctgtcacc ggatgtgctt tccggtctga tgagtccgtg aggacgaaac agcctctaca
     9001 aataattttg tttaacttta caaagaggag aaaggtaccg catgagtaaa ggagaagaac
     9061 ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat
     9121 tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattga
     9181 tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact ttgggttatg
     9241 gtgttcaatg ctttgcgaga tacccagatc atatgaaaca gcatgacttt ttcaagagtg
     9301 ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca
     9361 agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag
     9421 gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac aactataact
     9481 cacacaatgt atacatcacg gcagacaaac aaaagaatgg aatcaaagcg aacttcaaaa
     9541 ttagacacaa cattgaagat ggaggtgttc aactagcaga ccattatcaa caaaatactc
     9601 caattggcga tggccctgtc cttttaccag acaaccatta cctgtcctac caatctaagc
     9661 tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg
     9721 ctgggattac acatggcatg gatgaactat acaaataagc ttgatatcga attcctgcag
     9781 cccgggggat cccattgtac gcgtgctaga ggcatcaaat aaaacgaaag gctcagtcga
     9841 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa
     9901 atactagagc ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac
     9961 aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc
    10021 gatatcaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat
    10081 tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag
    10141 caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc
    10201 catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa
    10261 aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac
    10321 atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga
    10381 tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat
    10441 caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc
    10501 aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa
    10561 ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc
    10621 ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt
    10681 tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg
    10741 tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgccgat caacgtctca
    10801 ttttcgccag atatc
//