LOCUS       pJK503_dCpRv1_dCpf1_PA4 9573 bp ds-DNA     circular     23-JUN-2020
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     misc_feature       6099..6118
                        /label="fnCpf1 direct repeat (Zetsche 2015)"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
     misc_feature       6119..6138
                        /label="crRNA_TA4"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     misc_feature       5892..5911
                        /label="crRNA_TA2"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
     primer_bind        5525..5543
                        /label="pBRforEco"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
     misc_feature       5872..5891
                        /label="fnCpf1 direct repeat (Zetsche 2015)"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
     CDS                complement(1..660)
                        /label="CmR"
                        /ApEinfo_revcolor=#f8d3a9
                        /ApEinfo_fwdcolor=#f8d3a9
     misc_feature       5975..6055
                        /label="PlmJ (Lou 2013)"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     misc_feature       5653..5672
                        /label="DR_FnCpf1"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
     misc_feature       6031..6238
                        /label="JKgB17"
                        /ApEinfo_revcolor=#9eafd2
                        /ApEinfo_fwdcolor=#9eafd2
     Terminator         6139..6195
                        /label="L3S2P55"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
     misc_feature       5912..5968
                        /label="L3S2P11 (error in SI)"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
     misc_feature       5837..5871
                        /label="P_A4"
                        /ApEinfo_revcolor=#d6b295
                        /ApEinfo_fwdcolor=#d6b295
     CDS                complement(793..1416)
                        /label="TetR"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
     misc_feature       8458..8470
                        /label="A4 oper"
                        /ApEinfo_revcolor=#d6b295
                        /ApEinfo_fwdcolor=#d6b295
     primer_bind        575..594
                        /label="CAT-R"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
     misc_feature       5399..5864
                        /label="JKgB15"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     CDS                complement(6722..7672)
                        /label="rep101 CDS"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
     misc_feature       join(6139..9573,1..1525)
                        /label="from pJK472"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
     primer_bind        complement(8616..8644)
                        /label="GFP-R"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
     misc_feature       6204..8433
                        /label="SC101 ori (from LPT119)"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     misc_feature       8434..8439
                        /label="AvrII/SpeI scar"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     misc_feature       4274..4276
                        /label="D917A"
                        /ApEinfo_revcolor=#ff9ccd
                        /ApEinfo_fwdcolor=#ff9ccd
     protein_bind       1471..1489
                        /label="tet operator"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
     misc_feature       8450..8484
                        /label="P_A4"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
     rep_origin         complement(7720..7942)
                        /label="pSC101 ori"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
     ribozyme insulator 5754..5832
                        /label="SarJ (Lou 2013)"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
     misc_feature       6099..6118
                        /label="DR_FnCpf1"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
     misc_RNA           8487..8536
                        /label="sTRSV HHRz"
                        /ApEinfo_revcolor=#d6b295
                        /ApEinfo_fwdcolor=#d6b295
     misc_feature       9447..9452
                        /label="SpeI/XbaI scar"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
     misc_feature       5872..5891
                        /label="DR_FnCpf1"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
     terminator         9360..9446
                        /label="rrnB T1 terminator"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
     terminator         5547..5590
                        /label="bacterial terminator"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
     primer_bind        9239..9259
                        /label="GFP-F"
                        /ApEinfo_revcolor=#75c6a9
                        /ApEinfo_fwdcolor=#75c6a9
     misc_feature       1526..5428
                        /label="FndCas12a"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
     misc_feature       5653..5672
                        /label="fnCpf1 direct repeat (Zetsche 2015)"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
     promoter           complement(661..763)
                        /label="cat promoter"
                        /ApEinfo_revcolor=#9eafd2
                        /ApEinfo_fwdcolor=#9eafd2
     Terminator         5693..5753
                        /label="L3S2P21"
                        /ApEinfo_revcolor=#75c6a9
                        /ApEinfo_fwdcolor=#75c6a9
     promoter           1435..1490
                        /label="tetR/tetA promoter s"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
     terminator         5429..5518
                        /label="ECK120029600 (Chen 2013)"
                        /ApEinfo_revcolor=#ff9ccd
                        /ApEinfo_fwdcolor=#ff9ccd
     primer_bind        1324..1343
                        /label="Tet-R"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     misc_feature       8586..9302
                        /label="Venus"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     misc_feature       5673..5692
                        /label="crRNA_TA1"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
     RBS                8566..8577
                        /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
                        /ApEinfo_revcolor=#9eafd2
                        /ApEinfo_fwdcolor=#9eafd2
     misc_feature       5830..6063
                        /label="JKgB16"
                        /ApEinfo_revcolor=#d6b295
                        /ApEinfo_fwdcolor=#d6b295
     misc_feature       6064..6098
                        /label="P_A1"
                        /ApEinfo_revcolor=#d6b295
                        /ApEinfo_fwdcolor=#d6b295
     RBS                1508..1519
                        /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     misc_feature       5618..5652
                        /label="P_A2"
                        /ApEinfo_revcolor=#f8d3a9
                        /ApEinfo_fwdcolor=#f8d3a9
     terminator         9459..9553
                        /label="lambda t0 terminator"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
     promoter           8485..8559
                        /label="RiboJ"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     primer             complement(5494..5549)
                        /label="502-R"
                        /note="sequence: aattctgcctcgtgatacgcctattAATTAAttcagccaaaaaacttaagaccgcc"
                        /ApEinfo_revcolor=#f8d3a9
                        /ApEinfo_fwdcolor=#f8d3a9
     primer             complement(6230..6249)
                        /label="SC101seqR"
                        /note="sequence: actagcaacaccagaacagc"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
     primer             5527..5548
                        /label="AHC138-end-F"
                        /note="sequence: taggcgtatcacgaggcagaat"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
     primer             complement(6223..6242)
                        /label="SC101-R"
                        /note="sequence: acaccagaacagcccgtttg"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
     primer             complement(1508..1537)
                        /label="500-vR"
                        /note="sequence: ataaattgacatagatcctttctcctcttt"
                        /ApEinfo_revcolor=#d59687
                        /ApEinfo_fwdcolor=#d59687
     primer             complement(6223..6242)
                        /label="119-genomicINT-Rv"
                        /note="sequence: acaccagaacagcccgtttg"
                        /ApEinfo_revcolor=#d59687
                        /ApEinfo_fwdcolor=#d59687
     primer             5399..5415
                        /label="gB15-F"
                        /note="sequence: tttgagttcgtgcagaa"
                        /ApEinfo_revcolor=#75c6a9
                        /ApEinfo_fwdcolor=#75c6a9
     primer             complement(7920..7944)
                        /label="119_ori-Rv"
                        /note="sequence: gtgagttatacacagggctgggatc"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
     primer             complement(5846..5864)
                        /label="gB15-R"
                        /note="sequence: taataccAGCTAGTTGTGG"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
     primer             complement(4578..4596)
                        /label="dCpfseqR"
                        /note="sequence: tagacctgcttctctacct"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
     primer             complement(8611..8631)
                        /label="VenusR"
                        /note="sequence: caagaattgggacaactccag"
                        /ApEinfo_revcolor=#9eafd2
                        /ApEinfo_fwdcolor=#9eafd2
     primer             7920..7939
                        /label="119_ori-F"
                        /note="sequence: gatcccagccctgtgtataa"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     primer             complement(5402..5427)
                        /label="500-Cp-2R"
                        /note="sequence: tagttattcctattctgcacgaactc"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
     primer             complement(4247..4296)
                        /label="500-Cp-R"
                        /note="sequence: gctaaatgtctttcacctctaGctatacttaatatatgaacatcatttgc"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
     primer             5513..5546
                        /label="SpeI-398-F"
                        /note="sequence: ATATATactagtaataggcgtatcacgaggcaga"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
     primer             complement(8413..8439)
                        /label="382-SC101-R"
                        /note="sequence: attggtgagaatccaagcactagtccaagcactagggacagtaag"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     primer             complement(1508..1528)
                        /label="461-chk-R"
                        /note="sequence: ccatagatcctttctcctcttt"
                        /ApEinfo_revcolor=#75c6a9
                        /ApEinfo_fwdcolor=#75c6a9
     primer             4271..4296
                        /label="500-Cp-2F"
                        /note="sequence: atagCtagaggtgaaagacatttagc"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
     primer             5402..5453
                        /label="502-F"
                        /note="sequence: gagttcgtgcagaataggaataactaattgagaagagaaaagaaaaccgccg"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
     primer             complement(join(9542..9573,1..13))
                        /label="378-t0-Rv"
                        /note="sequence: gggcggggcgtaatttgatatcgagctcgcttggactcctgttga"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
     primer             6196..6222
                        /label="500-vF"
                        /note="sequence: TTAATTAAgtacgggttttgctgcccg"
                        /ApEinfo_revcolor=#ff9ccd
                        /ApEinfo_fwdcolor=#ff9ccd
     primer             complement(5518..5543)
                        /label="502seqR"
                        /note="sequence: gcctcgtgatacgcctattAATTAAt"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
     primer             1520..1547
                        /label="500-Cp-F"
                        /note="sequence: ggatctatgtcaatttatcaagaatttg"
                        /ApEinfo_revcolor=#ff9ccd
                        /ApEinfo_fwdcolor=#ff9ccd
     primer             complement(6535..6554)
                        /label="414-chk-Rv"
                        /note="sequence: aagctgttcaccatgaacag"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac
       61 atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc
      121 gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc
      181 cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt
      241 ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga
      301 atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt
      361 ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc
      421 accgtctttc attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg
      481 aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat
      541 atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg
      601 ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat
      661 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
      721 tatttcatta tggtgaaagt tggaacctct tacgtgccga tcaacgtctc attttcgcca
      781 gatatcgacg tcttaagacc cactttcaca tttaagttgt ttttctaatc cgcatatgat
      841 caattcaagg ccgaataaga aggctggctc tgcaccttgg tgatcaaata attcgatagc
      901 ttgtcgtaat aatggcggca tactatcagt agtaggtgtt tccctttctt ctttagcgac
      961 ttgatgctct tgatcttcca atacgcaacc taaagtaaaa tgccccacag cgctgagtgc
     1021 atataatgca ttctctagtg aaaaaccttg ttggcataaa aaggctaatt gattttcgag
     1081 agtttcatac tgtttttctg taggccgtgt acctaaatgt acttttgctc catcgcgatg
     1141 acttagtaaa gcacatctaa aacttttagc gttattacgt aaaaaatctt gccagctttc
     1201 cccttctaaa gggcaaaagt gagtatggtg cctatctaac atctcaatgg ctaaggcgtc
     1261 gagcaaagcc cgcttatttt ttacatgcca atacaatgta ggctgctcta cacctagctt
     1321 ctgggcgagt ttacgggttg ttaaaccttc gattccgacc tcattaagca gctctaatgc
     1381 gctgttaatc actttacttt tatctaatct agacatcatt aattcctaat ttttgttgac
     1441 actctatcgt tgatagagtt attttaccac tccctatcag tgatagagaa aagaattcaa
     1501 aagatctaaa gaggagaaag gatctatgtc aatttatcaa gaatttgtta ataaatatag
     1561 tttaagtaaa actctaagat ttgagttaat cccacagggt aaaacacttg aaaacataaa
     1621 agcaagaggt ttgattttag atgatgagaa aagagctaaa gactacaaaa aggctaaaca
     1681 aataattgat aaatatcatc agttttttat agaggagata ttaagttcgg tttgtattag
     1741 cgaagattta ttacaaaact attctgatgt ttattttaaa cttaaaaaga gtgatgatga
     1801 taatctacaa aaagatttta aaagtgcaaa agatacgata aagaaacaaa tatctgaata
     1861 tataaaggac tcagagaaat ttaagaattt gtttaatcaa aaccttatcg atgctaaaaa
     1921 agggcaagag tcagatttaa ttctatggct aaagcaatct aaggataatg gtatagaact
     1981 atttaaagcc aatagtgata tcacagatat agatgaggcg ttagaaataa tcaaatcttt
     2041 taaaggttgg acaacttatt ttaagggttt tcatgaaaat agaaaaaatg tttatagtag
     2101 caatgatatt cctacatcta ttatttatag gatagtagat gataatttgc ctaaatttct
     2161 agaaaataaa gctaagtatg agagtttaaa agacaaagct ccagaagcta taaactatga
     2221 acaaattaaa aaagatttgg cagaagagct aacctttgat attgactaca aaacatctga
     2281 agttaatcaa agagtttttt cacttgatga agtttttgag atagcaaact ttaataatta
     2341 tctaaatcaa agtggtatta ctaaatttaa tactattatt ggtggtaaat ttgtaaatgg
     2401 tgaaaataca aagagaaaag gtataaatga atatataaat ctatactcac agcaaataaa
     2461 tgataaaaca ctcaaaaaat ataaaatgag tgttttattt aagcaaattt taagtgatac
     2521 agaatctaaa tcttttgtaa ttgataagtt agaagatgat agtgatgtag ttacaacgat
     2581 gcaaagtttt tatgagcaaa tagcagcttt taaaacagta gaagaaaaat ctattaaaga
     2641 aacactatct ttattatttg atgatttaaa agctcaaaaa cttgatttga gtaaaattta
     2701 ttttaaaaat gataaatctc ttactgatct atcacaacaa gtttttgatg attatagtgt
     2761 tattggtaca gcggtactag aatatataac tcaacaaata gcacctaaaa atcttgataa
     2821 ccctagtaag aaagagcaag aattaatagc caaaaaaact gaaaaagcaa aatacttatc
     2881 tctagaaact ataaagcttg ccttagaaga atttaataag catagagata tagataaaca
     2941 gtgtaggttt gaagaaatac ttgcaaactt tgcggctatt ccgatgatat ttgatgaaat
     3001 agctcaaaac aaagacaatt tggcacagat atctatcaaa tatcaaaatc aaggtaaaaa
     3061 agacctactt caagctagtg cggaagatga tgttaaagct atcaaggatc ttttagatca
     3121 aactaataat ctcttacata aactaaaaat atttcatatt agtcagtcag aagataaggc
     3181 aaatatttta gacaaggatg agcattttta tctagtattt gaggagtgct actttgagct
     3241 agcgaatata gtgcctcttt ataacaaaat tagaaactat ataactcaaa agccatatag
     3301 tgatgagaaa tttaagctca attttgagaa ctcgactttg gctaatggtt gggataaaaa
     3361 taaagagcct gacaatacgg caattttatt tatcaaagat gataaatatt atctgggtgt
     3421 gatgaataag aaaaataaca aaatatttga tgataaagct atcaaagaaa ataaaggcga
     3481 gggttataaa aaaattgttt ataaactttt acctggcgca aataaaatgt tacctaaggt
     3541 tttcttttct gctaaatcta taaaatttta taatcctagt gaagatatac ttagaataag
     3601 aaatcattcc acacatacaa aaaatggtag tcctcaaaaa ggatatgaaa aatttgagtt
     3661 taatattgaa gattgccgaa aatttataga tttttataaa cagtctataa gtaagcatcc
     3721 ggagtggaaa gattttggat ttagattttc tgatactcaa agatataatt ctatagatga
     3781 attttataga gaagttgaaa atcaaggcta caaactaact tttgaaaata tatcagagag
     3841 ctatattgat agcgtagtta atcagggtaa attgtaccta ttccaaatct ataataaaga
     3901 tttttcagct tatagcaaag ggcgaccaaa tctacatact ttatattgga aagcgctgtt
     3961 tgatgagaga aatcttcaag atgtggttta taagctaaat ggtgaggcag agctttttta
     4021 tcgtaaacaa tcaataccta aaaaaatcac tcacccagct aaagaggcaa tagctaataa
     4081 aaacaaagat aatcctaaaa aagagagtgt ttttgaatat gatttaatca aagataaacg
     4141 ctttactgaa gataagtttt tctttcactg tcctattaca atcaatttta aatctagtgg
     4201 agctaataag tttaatgatg aaatcaattt attgctaaaa gaaaaagcaa atgatgttca
     4261 tatattaagt atagCtagag gtgaaagaca tttagcttac tatactttgg tagatggtaa
     4321 aggcaatatc atcaaacaag atactttcaa catcattggt aatgatagaa tgaaaacaaa
     4381 ctaccatgat aagcttgctg caatagagaa agatagggat tcagctagga aagactggaa
     4441 aaagataaat aacatcaaag agatgaaaga gggctatcta tctcaggtag ttcatgaaat
     4501 agctaagcta gttatagagt ataatgctat tgtggttttt gaggatttaa attttggatt
     4561 taaaagaggg cgtttcaagg tagagaagca ggtctatcaa aagttagaaa aaatgctaat
     4621 tgagaaacta aactatctag ttttcaaaga taatgagttt gataaaactg ggggagtgct
     4681 tagagcttat cagctaacag caccttttga gacttttaaa aagatgggta aacaaacagg
     4741 tattatctac tatgtaccag ctggttttac ttcaaaaatt tgtcctgtaa ctggttttgt
     4801 aaatcagtta tatcctaagt atgaaagtgt cagcaaatct caagagttct ttagtaagtt
     4861 tgacaagatt tgttataacc ttgataaggg ctattttgag tttagttttg attataaaaa
     4921 ctttggtgac aaggctgcca aaggcaagtg gactatagct agctttggga gtagattgat
     4981 taactttaga aattcagata aaaatcataa ttgggatact cgagaagttt atccaactaa
     5041 agagttggag aaattgctaa aagattattc tatcgaatat gggcatggcg aatgtatcaa
     5101 agcagctatt tgcggtgaga gcgacaaaaa gttttttgct aagctaacta gtgtcctaaa
     5161 tactatctta caaatgcgta actcaaaaac aggtactgag ttagattatc taatttcacc
     5221 agtagcagat gtaaatggca atttctttga ttcgcgacag gcgccaaaaa atatgcctca
     5281 agatgctgat gccaatggtg cttatcatat tgggctaaaa ggtctgatgc tactaggtag
     5341 gatcaaaaat aatcaagagg gcaaaaaact caatttggtt atcaaaaatg aagagtattt
     5401 tgagttcgtg cagaatagga ataactaatt gagaagagaa aagaaaaccg ccgatcctgt
     5461 ccaccgcatt actgcaaggt agtggacaag accggcggtc ttaagttttt tggctgaaTT
     5521 AATTaatagg cgtatcacga ggcagaattt cagataaaaa aaatccttag ctttcgctaa
     5581 ggatgatttc tggaattcta aagatctcag aaatcatttt acaccAACGG GTCACACGgg
     5641 tattatgcta gcTAATTTCT ACTGTTGTAG ATcaccTAGC TCAGTCCTAg gtctcggtac
     5701 caaattccag aaaagaggcc tcccgaaagg ggggcctttt ttcgttttgg tccAGACTGT
     5761 CGCCGGATGT GTATCCGACC TGACGATGGC CCAAAAGGGC CGAAACAGTC CTCTACAAAT
     5821 AATTTTGTTT AAgcttttta caccTCCACA ACTAGCTggt attatgctag cTAATTTCTA
     5881 CTGTTGTAGA TcaccAACGG GTCACACGgg tctcggtacc aaattccaga aaagagacgc
     5941 tttcgagcgt cttttttcgt tttggtccgg atccAGTCAT AAGTCTGGGC TAAGCCCACT
     6001 GATGAGTCGC TGAAATGCGA CGAAACTTAT GACCTCTACA AATAATTTTG TTTAAgatag
     6061 gattttacac cTAGCTCAGT CCTAggtatt atgctagcTA ATTTCTACTG TTGTAGATca
     6121 ccTCCACAAC TAGCTggtct cggtaccaaa gacgaacaat aagacgctga aaagcgtctt
     6181 ttttcgtttt ggtccTTAAT TAAgtacggg ttttgctgcc cgcaaacggg ctgttctggt
     6241 gttgctagtt tgttatcaga atcgcagatc cggcttcagc cggtttgccg gctgaaagcg
     6301 ctatttcttc cagaattgcc atgatttttt ccccacggga ggcgtcactg gctcccgtgt
     6361 tgtcggcagc tttgattcga taagcagcat cgcctgtttc aggctgtcta tgtgtgactg
     6421 ttgagctgta acaagttgtc tcaggtgttc aatttcatgt tctagttgct ttgttttact
     6481 ggtttcacct gttctattag gtgttacatg ctgttcatct gttacattgt cgatctgttc
     6541 atggtgaaca gctttgaatg caccaaaaac tcgtaaaagc tctgatgtat ctatcttttt
     6601 tacaccgttt tcatctgtgc atatggacag ttttcccttt gatatgtaac ggtgaacagt
     6661 tgttctactt ttgtttgtta gtcttgatgc ttcactgata gatacaagag ccataagaac
     6721 ctcagatcct tccgtattta gccagtatgt tctctagtgt ggttcgttgt ttttgcgtga
     6781 gccatgagaa cgaaccattg agatcatact tactttgcat gtcactcaaa aattttgcct
     6841 caaaactggt gagctgaatt tttgcagtta aagcatcgtg tagtgttttt cttagtccgt
     6901 tatgtaggta ggaatctgat gtaatggttg ttggtatttt gtcaccattc atttttatct
     6961 ggttgttctc aagttcggtt acgagatcca tttgtctatc tagttcaact tggaaaatca
     7021 acgtatcagt cgggcggcct cgcttatcaa ccaccaattt catattgctg taagtgttta
     7081 aatctttact tattggtttc aaaacccatt ggttaagcct tttaaactca tggtagttat
     7141 tttcaagcat taacatgaac ttaaattcat caaggctaat ctctatattt gccttgtgag
     7201 ttttcttttg tgttagttct tttaataacc actcataaat cctcatagag tatttgtttt
     7261 caaaagactt aacatgttcc agattatatt ttatgaattt ttttaactgg aaaagataag
     7321 gcaatatctc ttcactaaaa actaattcta atttttcgct tgagaacttg gcatagtttg
     7381 tccactggaa aatctcaaag cctttaacca aaggattcct gatttccaca gttctcgtca
     7441 tcagctctct ggttgcttta gctaatacac cataagcatt ttccctactg atgttcatca
     7501 tctgagcgta ttggttataa gtgaacgata ccgtccgttc tttccttgta gggttttcaa
     7561 tcgtggggtt gagtagtgcc acacagcata aaattagctt ggtttcatgc tccgttaagt
     7621 catagcgact aatcgctagt tcatttgctt tgaaaacaac taattcagac atacatctca
     7681 attggtctag gtgattttaa tcactatacc aattgagatg ggctagtcaa tgataattac
     7741 tagtcctttt cctttgagtt gtgggtatct gtaaattctg ctagaccttt gctggaaaac
     7801 ttgtaaattc tgctagaccc tctgtaaatt ccgctagacc tttgtgtgtt ttttttgttt
     7861 atattcaagt ggttataatt tatagaataa agaaagaata aaaaaagata aaaagaatag
     7921 atcccagccc tgtgtataac tcactacttt agtcagttcc gcagtattac aaaaggatgt
     7981 cgcaaacgct gtttgctcct ctacaaaaca gaccttaaaa ccctaaaggc ttaagtagca
     8041 ccctcgcaag ctcgggcaaa tcgctgaata ttccttttgt ctccgaccat caggcacctg
     8101 agtcgctgtc tttttcgtga cattcagttc gctgcgctca cggctctggc agtgaatggg
     8161 ggtaaatggc actacaggcg ccttttatgg attcatgcaa ggaaactacc cataatacaa
     8221 gaaaagcccg tcacgggctt ctcagggcgt tttatggcgg gtctgctatg tggtgctatc
     8281 tgactttttg ctgttcagca gttcctgccc tctgattttc cagtctgacc acttcggatt
     8341 atcccgtgac aggtcattca gactggctaa tgcacccagt aaggcagcgg tatcatcaac
     8401 aggcttaccc gtcttactgt ccctagtgct tggcctagta tgcgtctcct ttacacctcc
     8461 acaactagct ggtattatgc tagcagctgt caccggatgt gctttccggt ctgatgagtc
     8521 cgtgaggacg aaacagcctc tacaaataat tttgtttaac tttacaaaga ggagaaaggt
     8581 accgcatgag taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag
     8641 atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat
     8701 acggaaaact tacccttaaa ttgatttgca ctactggaaa actacctgtt ccatggccaa
     8761 cacttgtcac tactttgggt tatggtgttc aatgctttgc gagataccca gatcatatga
     8821 aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat
     8881 ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc
     8941 ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac
     9001 acaaattgga atacaactat aactcacaca atgtatacat cacggcagac aaacaaaaga
     9061 atggaatcaa agcgaacttc aaaattagac acaacattga agatggaggt gttcaactag
     9121 cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc
     9181 attacctgtc ctaccaatct aagctttcga aagatcccaa cgaaaagaga gaccacatgg
     9241 tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa ctatacaaat
     9301 aagcttgata tcgaattcct gcagcccggg ggatcccatt gtacgcgtgc tagaggcatc
     9361 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     9421 tgaacgctct cctgagtagg acaaatacta gagcttggat tctcaccaat aaaaaacgcc
     9481 cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct
     9541 atcaacagga gtccaagcga gctcgatatc aaa
//