LOCUS       pJK504_crRNA_dCpRv1_(p1 2639 bp ds-DNA     circular     04-JAN-2020
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     misc_feature       1029..1048
                        /label="fnCpf1 direct repeat (Zetsche 2015)"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
     misc_feature       1946..1985
                        /label="found in LPT41"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
     misc_feature       905..985
                        /label="PlmJ (Lou 2013)"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     misc_feature       802..821
                        /label="DR_FnCpf1"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
     rep_origin         complement(1279..1824)
                        /label="p15A ori"
                        /ApEinfo_revcolor=#9eafd2
                        /ApEinfo_fwdcolor=#9eafd2
     CDS                complement(join(2080..2639,1..301))
                        /label="AmpR"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
     primer_bind        64..83
                        /label="Amp-R"
                        /ApEinfo_revcolor=#d6b295
                        /ApEinfo_fwdcolor=#d6b295
     promoter           complement(302..406)
                        /label="AmpR promoter"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
     misc_feature       1029..1048
                        /label="DR_FnCpf1"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
     misc_feature       449..1133
                        /label="from pJK500 via PacI"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
     misc_feature       583..602
                        /label="fnCpf1 direct repeat (Zetsche 2015)"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
     misc_feature       583..602
                        /label="DR_FnCpf1"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
     primer_bind        complement(1145..1162)
                        /label="L4440"
                        /ApEinfo_revcolor=#d59687
                        /ApEinfo_fwdcolor=#d59687
     misc_feature       1049..1068
                        /label="crRNA_TA4"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     misc_feature       842..898
                        /label="L3S2P11 (error in SI)"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
     misc_feature       822..841
                        /label="crRNA_TA2"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
     terminator         477..520
                        /label="bacterial terminator"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
     Terminator         623..683
                        /label="L3S2P21"
                        /ApEinfo_revcolor=#75c6a9
                        /ApEinfo_fwdcolor=#75c6a9
     misc_feature       767..801
                        /label="P_A4"
                        /ApEinfo_revcolor=#d6b295
                        /ApEinfo_fwdcolor=#d6b295
     Terminator         1069..1125
                        /label="L3S2P55"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
     misc_feature       603..622
                        /label="crRNA_TA1"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
     primer_bind        455..473
                        /label="pBRforEco"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
     misc_feature       548..582
                        /label="P_A2"
                        /ApEinfo_revcolor=#f8d3a9
                        /ApEinfo_fwdcolor=#f8d3a9
     misc_feature       802..821
                        /label="fnCpf1 direct repeat (Zetsche 2015)"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
     misc_feature       join(1123..2639,1..454)
                        /label="from pJK398"
                        /ApEinfo_revcolor=#d6b295
                        /ApEinfo_fwdcolor=#d6b295
     ribozyme insulator 684..762
                        /label="SarJ (Lou 2013)"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
     primer_bind        complement(1359..1378)
                        /label="pBR322ori-F"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
     misc_feature       760..993
                        /label="JKgB16"
                        /ApEinfo_revcolor=#d6b295
                        /ApEinfo_fwdcolor=#d6b295
     misc_feature       994..1028
                        /label="P_A1"
                        /ApEinfo_revcolor=#d6b295
                        /ApEinfo_fwdcolor=#d6b295
     misc_feature       1145..1945
                        /label="p15A from pdCas9"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     primer             217..242
                        /label="mid-Amp-F2"
                        /note="sequence: ccagcgtttctgggtgagcaaaaaca"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
     primer             758..789
                        /label="386-BgH-F"
                        /note="sequence: agatctaagctttttacacctccacaactagctgg"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
     primer             complement(881..899)
                        /label="386-Bam-R"
                        /note="sequence: ggatcctatcggaccaaaacgaaaaaag"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
     primer             complement(1121..1150)
                        /label="514-SpeI-R2"
                        /note="sequence: AATTACTAGTcctgcagTTAATTAAggacc"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     primer             443..476
                        /label="SpeI-398-F"
                        /note="sequence: ATATATactagtaataggcgtatcacgaggcaga"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
     primer             complement(1926..1965)
                        /label="398-p15A-R"
                        /note="sequence: cgtgagttttcgttccactgccaagcactagtaacaactt"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     primer             548..567
                        /label="513-Bss-F"
                        /note="sequence: TATAgcgcgctttacaccaacgggtcacac"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     primer             complement(2060..2079)
                        /label="pKD46-back-R"
                        /note="sequence: ctgtcagaccaagtttactc"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
     primer             532..560
                        /label="385-BglII-F"
                        /note="sequence: agatctcagaaatcattttacaccaacgg"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
     primer             complement(1210..1229)
                        /label="433end-R"
                        /note="sequence: atcttccaggaaatctccgc"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     primer             1640..1659
                        /label="p15a-F"
                        /note="sequence: tagtcttgaagtcatgcgcc"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
     primer             complement(217..242)
                        /label="mid-Amp-Rv"
                        /note="sequence: tgtttttgctcacccagaaacgctgg"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
     primer             1132..1164
                        /label="398-p15A-F"
                        /note="sequence: gactgcaggcttcctcgctcactgactcgctac"
                        /ApEinfo_revcolor=#ff9ccd
                        /ApEinfo_fwdcolor=#ff9ccd
     primer             complement(429..459)
                        /label="504-v-R"
                        /note="sequence: ccgTTAATTAAatggtttcttagacgtcagg"
                        /ApEinfo_revcolor=#ff9ccd
                        /ApEinfo_fwdcolor=#ff9ccd
     primer             complement(776..794)
                        /label="gB15-R"
                        /note="sequence: taataccAGCTAGTTGTGG"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
     primer             457..478
                        /label="AHC138-end-F"
                        /note="sequence: taggcgtatcacgaggcagaat"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
     primer             complement(877..898)
                        /label="387-Xba-R"
                        /note="sequence: tctagataaaggaccaaaacgaaaaaagacgc"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     primer             1931..1965
                        /label="398-388-F"
                        /note="sequence: gttactagtgcttggcagtggaacgaaaactcacg"
                        /ApEinfo_revcolor=#75c6a9
                        /ApEinfo_fwdcolor=#75c6a9
     primer             215..240
                        /label="mid-Amp-F"
                        /note="sequence: caccagcgtttctgggtgagcaaaaa"
                        /ApEinfo_revcolor=#d6b295
                        /ApEinfo_fwdcolor=#d6b295
     primer             404..422
                        /label="2u-ins-R"
                        /note="sequence: gcgcacatttccccgaaaa"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
     primer             1123..1164
                        /label="504-v-F"
                        /note="sequence: ccgTTAATTAActgcaggcttcctcgctcactgactcgctac"
                        /ApEinfo_revcolor=#d6b295
                        /ApEinfo_fwdcolor=#d6b295
ORIGIN
        1 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
       61 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
      121 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
      181 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
      241 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
      301 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
      361 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
      421 aagtgccacc tgacgtctaa gaaaccatTT AATTaatagg cgtatcacga ggcagaattt
      481 cagataaaaa aaatccttag ctttcgctaa ggatgatttc tggaattcta aagatctcag
      541 aaatcatttt acaccAACGG GTCACACGgg tattatgcta gcTAATTTCT ACTGTTGTAG
      601 ATcaccTAGC TCAGTCCTAg gtctcggtac caaattccag aaaagaggcc tcccgaaagg
      661 ggggcctttt ttcgttttgg tccAGACTGT CGCCGGATGT GTATCCGACC TGACGATGGC
      721 CCAAAAGGGC CGAAACAGTC CTCTACAAAT AATTTTGTTT AAgcttttta caccTCCACA
      781 ACTAGCTggt attatgctag cTAATTTCTA CTGTTGTAGA TcaccAACGG GTCACACGgg
      841 tctcggtacc aaattccaga aaagagacgc tttcgagcgt cttttttcgt tttggtccgg
      901 atccAGTCAT AAGTCTGGGC TAAGCCCACT GATGAGTCGC TGAAATGCGA CGAAACTTAT
      961 GACCTCTACA AATAATTTTG TTTAAgatag gattttacac cTAGCTCAGT CCTAggtatt
     1021 atgctagcTA ATTTCTACTG TTGTAGATca ccTCCACAAC TAGCTggtct cggtaccaaa
     1081 gacgaacaat aagacgctga aaagcgtctt ttttcgtttt ggtccTTAAT TAActgcagg
     1141 cttcctcgct cactgactcg ctacgctcgg tcgttcgact gcggcgagcg gaaatggctt
     1201 acgaacgggg cggagatttc ctggaagatg ccaggaagat acttaacagg gaagtgagag
     1261 ggccgcggca aagccgtttt tccataggct ccgcccccct gacaagcatc acgaaatctg
     1321 acgctcaaat cagtggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     1381 tggcggctcc ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg tcattccgct
     1441 gttatggccg cgtttgtctc attccacgcc tgacactcag ttccgggtag gcagttcgct
     1501 ccaagctgga ctgtatgcac gaaccccccg ttcagtccga ccgctgcgcc ttatccggta
     1561 actatcgtct tgagtccaac ccggaaagac atgcaaaagc accactggca gcagccactg
     1621 gtaattgatt tagaggagtt agtcttgaag tcatgcgccg gttaaggcta aactgaaagg
     1681 acaagttttg gtgactgcgc tcctccaagc cagttacctc ggttcaaaga gttggtagct
     1741 cagagaacct tcgaaaaacc gccctgcaag gcggtttttt cgttttcaga gcaagagatt
     1801 acgcgcagac caaaacgatc tcaagaagat catcttatta atcagataaa atatttctag
     1861 atttcagtgc aatttatctc ttcaaatgta gcacctgaag tcagccccat acgatataag
     1921 ttgttaagtt gttactagtg cttggcagtg gaacgaaaac tcacgttaag ggattttggt
     1981 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     2041 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     2101 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     2161 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     2221 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     2281 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     2341 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     2401 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     2461 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     2521 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     2581 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaa
//