LOCUS       pJK506_dCas12a_PA4-Venu 8896 bp ds-DNA     circular     23-JUN-2020
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     CDS             complement(1..660)
                     /label="CmR"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    7757..7762
                     /label="AvrII/SpeI scar"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    4274..4276
                     /label="D917A"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     RBS             7889..7900
                     /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     RBS             1508..1519
                     /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     terminator      8683..8769
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     terminator      8782..8876
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        7808..7882
                     /label="RiboJ"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        complement(661..763)
                     /label="cat promoter"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer_bind     1324..1343
                     /label="Tet-R"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    join(5519..8896,1..1525)
                     /label="from pJK472"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    5519..5561
                     /label="JKgB17"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     promoter        1435..1490
                     /label="tetR/tetA promoter s"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     terminator      5429..5518
                     /label="ECK120029600 (Chen 2013)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    8770..8775
                     /label="SpeI/XbaI scar"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     575..594
                     /label="CAT-R"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     protein_bind    1471..1489
                     /label="tet operator"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    7909..8625
                     /label="Venus"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1526..5428
                     /label="FndCas12a"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer_bind     complement(7939..7967)
                     /label="GFP-R"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     rep_origin      complement(7043..7265)
                     /label="pSC101 ori"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             complement(793..1416)
                     /label="TetR"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    7781..7793
                     /label="A4 oper"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             complement(6045..6995)
                     /label="rep101 CDS"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_RNA        7810..7859
                     /label="sTRSV HHRz"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    5399..5518
                     /label="JKgB15"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    5527..7756
                     /label="SC101 ori (from LPT119)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    7773..7807
                     /label="P_A4"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer_bind     8562..8582
                     /label="GFP-F"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(5494..5549)
                     /label="502-R"
                     /note="sequence: aattctgcctcgtgatacgcctattAATTAAttcagccaaaaaacttaagaccgcc"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          4271..4296
                     /label="500-Cp-2F"
                     /note="sequence: atagCtagaggtgaaagacatttagc"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          complement(4578..4596)
                     /label="dCpfseqR"
                     /note="sequence: tagacctgcttctctacct"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(1508..1537)
                     /label="500-vR"
                     /note="sequence: ataaattgacatagatcctttctcctcttt"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(5518..5543)
                     /label="502seqR"
                     /note="sequence: gcctcgtgatacgcctattAATTAAt"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          complement(5402..5427)
                     /label="500-Cp-2R"
                     /note="sequence: tagttattcctattctgcacgaactc"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(1508..1528)
                     /label="461-chk-R"
                     /note="sequence: ccatagatcctttctcctcttt"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(5553..5572)
                     /label="SC101seqR"
                     /note="sequence: actagcaacaccagaacagc"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          1520..1547
                     /label="500-Cp-F"
                     /note="sequence: ggatctatgtcaatttatcaagaatttg"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          7243..7262
                     /label="119_ori-F"
                     /note="sequence: gatcccagccctgtgtataa"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(5546..5565)
                     /label="SC101-R"
                     /note="sequence: acaccagaacagcccgtttg"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          complement(join(8865..8896,1..13))
                     /label="378-t0-Rv"
                     /note="sequence: gggcggggcgtaatttgatatcgagctcgcttggactcctgttga"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(4247..4296)
                     /label="500-Cp-R"
                     /note="sequence: gctaaatgtctttcacctctaGctatacttaatatatgaacatcatttgc"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(7934..7954)
                     /label="VenusR"
                     /note="sequence: caagaattgggacaactccag"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          5402..5453
                     /label="502-F"
                     /note="sequence: gagttcgtgcagaataggaataactaattgagaagagaaaagaaaaccgccg"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(7243..7267)
                     /label="119_ori-Rv"
                     /note="sequence: gtgagttatacacagggctgggatc"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(7736..7762)
                     /label="382-SC101-R"
                     /note="sequence: attggtgagaatccaagcactagtccaagcactagggacagtaag"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          5519..5545
                     /label="500-vF"
                     /note="sequence: TTAATTAAgtacgggttttgctgcccg"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(5546..5565)
                     /label="119-genomicINT-Rv"
                     /note="sequence: acaccagaacagcccgtttg"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          5399..5415
                     /label="gB15-F"
                     /note="sequence: tttgagttcgtgcagaa"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(5858..5877)
                     /label="414-chk-Rv"
                     /note="sequence: aagctgttcaccatgaacag"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac
       61 atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc
      121 gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc
      181 cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt
      241 ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga
      301 atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt
      361 ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc
      421 accgtctttc attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg
      481 aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat
      541 atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg
      601 ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat
      661 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
      721 tatttcatta tggtgaaagt tggaacctct tacgtgccga tcaacgtctc attttcgcca
      781 gatatcgacg tcttaagacc cactttcaca tttaagttgt ttttctaatc cgcatatgat
      841 caattcaagg ccgaataaga aggctggctc tgcaccttgg tgatcaaata attcgatagc
      901 ttgtcgtaat aatggcggca tactatcagt agtaggtgtt tccctttctt ctttagcgac
      961 ttgatgctct tgatcttcca atacgcaacc taaagtaaaa tgccccacag cgctgagtgc
     1021 atataatgca ttctctagtg aaaaaccttg ttggcataaa aaggctaatt gattttcgag
     1081 agtttcatac tgtttttctg taggccgtgt acctaaatgt acttttgctc catcgcgatg
     1141 acttagtaaa gcacatctaa aacttttagc gttattacgt aaaaaatctt gccagctttc
     1201 cccttctaaa gggcaaaagt gagtatggtg cctatctaac atctcaatgg ctaaggcgtc
     1261 gagcaaagcc cgcttatttt ttacatgcca atacaatgta ggctgctcta cacctagctt
     1321 ctgggcgagt ttacgggttg ttaaaccttc gattccgacc tcattaagca gctctaatgc
     1381 gctgttaatc actttacttt tatctaatct agacatcatt aattcctaat ttttgttgac
     1441 actctatcgt tgatagagtt attttaccac tccctatcag tgatagagaa aagaattcaa
     1501 aagatctaaa gaggagaaag gatctatgtc aatttatcaa gaatttgtta ataaatatag
     1561 tttaagtaaa actctaagat ttgagttaat cccacagggt aaaacacttg aaaacataaa
     1621 agcaagaggt ttgattttag atgatgagaa aagagctaaa gactacaaaa aggctaaaca
     1681 aataattgat aaatatcatc agttttttat agaggagata ttaagttcgg tttgtattag
     1741 cgaagattta ttacaaaact attctgatgt ttattttaaa cttaaaaaga gtgatgatga
     1801 taatctacaa aaagatttta aaagtgcaaa agatacgata aagaaacaaa tatctgaata
     1861 tataaaggac tcagagaaat ttaagaattt gtttaatcaa aaccttatcg atgctaaaaa
     1921 agggcaagag tcagatttaa ttctatggct aaagcaatct aaggataatg gtatagaact
     1981 atttaaagcc aatagtgata tcacagatat agatgaggcg ttagaaataa tcaaatcttt
     2041 taaaggttgg acaacttatt ttaagggttt tcatgaaaat agaaaaaatg tttatagtag
     2101 caatgatatt cctacatcta ttatttatag gatagtagat gataatttgc ctaaatttct
     2161 agaaaataaa gctaagtatg agagtttaaa agacaaagct ccagaagcta taaactatga
     2221 acaaattaaa aaagatttgg cagaagagct aacctttgat attgactaca aaacatctga
     2281 agttaatcaa agagtttttt cacttgatga agtttttgag atagcaaact ttaataatta
     2341 tctaaatcaa agtggtatta ctaaatttaa tactattatt ggtggtaaat ttgtaaatgg
     2401 tgaaaataca aagagaaaag gtataaatga atatataaat ctatactcac agcaaataaa
     2461 tgataaaaca ctcaaaaaat ataaaatgag tgttttattt aagcaaattt taagtgatac
     2521 agaatctaaa tcttttgtaa ttgataagtt agaagatgat agtgatgtag ttacaacgat
     2581 gcaaagtttt tatgagcaaa tagcagcttt taaaacagta gaagaaaaat ctattaaaga
     2641 aacactatct ttattatttg atgatttaaa agctcaaaaa cttgatttga gtaaaattta
     2701 ttttaaaaat gataaatctc ttactgatct atcacaacaa gtttttgatg attatagtgt
     2761 tattggtaca gcggtactag aatatataac tcaacaaata gcacctaaaa atcttgataa
     2821 ccctagtaag aaagagcaag aattaatagc caaaaaaact gaaaaagcaa aatacttatc
     2881 tctagaaact ataaagcttg ccttagaaga atttaataag catagagata tagataaaca
     2941 gtgtaggttt gaagaaatac ttgcaaactt tgcggctatt ccgatgatat ttgatgaaat
     3001 agctcaaaac aaagacaatt tggcacagat atctatcaaa tatcaaaatc aaggtaaaaa
     3061 agacctactt caagctagtg cggaagatga tgttaaagct atcaaggatc ttttagatca
     3121 aactaataat ctcttacata aactaaaaat atttcatatt agtcagtcag aagataaggc
     3181 aaatatttta gacaaggatg agcattttta tctagtattt gaggagtgct actttgagct
     3241 agcgaatata gtgcctcttt ataacaaaat tagaaactat ataactcaaa agccatatag
     3301 tgatgagaaa tttaagctca attttgagaa ctcgactttg gctaatggtt gggataaaaa
     3361 taaagagcct gacaatacgg caattttatt tatcaaagat gataaatatt atctgggtgt
     3421 gatgaataag aaaaataaca aaatatttga tgataaagct atcaaagaaa ataaaggcga
     3481 gggttataaa aaaattgttt ataaactttt acctggcgca aataaaatgt tacctaaggt
     3541 tttcttttct gctaaatcta taaaatttta taatcctagt gaagatatac ttagaataag
     3601 aaatcattcc acacatacaa aaaatggtag tcctcaaaaa ggatatgaaa aatttgagtt
     3661 taatattgaa gattgccgaa aatttataga tttttataaa cagtctataa gtaagcatcc
     3721 ggagtggaaa gattttggat ttagattttc tgatactcaa agatataatt ctatagatga
     3781 attttataga gaagttgaaa atcaaggcta caaactaact tttgaaaata tatcagagag
     3841 ctatattgat agcgtagtta atcagggtaa attgtaccta ttccaaatct ataataaaga
     3901 tttttcagct tatagcaaag ggcgaccaaa tctacatact ttatattgga aagcgctgtt
     3961 tgatgagaga aatcttcaag atgtggttta taagctaaat ggtgaggcag agctttttta
     4021 tcgtaaacaa tcaataccta aaaaaatcac tcacccagct aaagaggcaa tagctaataa
     4081 aaacaaagat aatcctaaaa aagagagtgt ttttgaatat gatttaatca aagataaacg
     4141 ctttactgaa gataagtttt tctttcactg tcctattaca atcaatttta aatctagtgg
     4201 agctaataag tttaatgatg aaatcaattt attgctaaaa gaaaaagcaa atgatgttca
     4261 tatattaagt atagCtagag gtgaaagaca tttagcttac tatactttgg tagatggtaa
     4321 aggcaatatc atcaaacaag atactttcaa catcattggt aatgatagaa tgaaaacaaa
     4381 ctaccatgat aagcttgctg caatagagaa agatagggat tcagctagga aagactggaa
     4441 aaagataaat aacatcaaag agatgaaaga gggctatcta tctcaggtag ttcatgaaat
     4501 agctaagcta gttatagagt ataatgctat tgtggttttt gaggatttaa attttggatt
     4561 taaaagaggg cgtttcaagg tagagaagca ggtctatcaa aagttagaaa aaatgctaat
     4621 tgagaaacta aactatctag ttttcaaaga taatgagttt gataaaactg ggggagtgct
     4681 tagagcttat cagctaacag caccttttga gacttttaaa aagatgggta aacaaacagg
     4741 tattatctac tatgtaccag ctggttttac ttcaaaaatt tgtcctgtaa ctggttttgt
     4801 aaatcagtta tatcctaagt atgaaagtgt cagcaaatct caagagttct ttagtaagtt
     4861 tgacaagatt tgttataacc ttgataaggg ctattttgag tttagttttg attataaaaa
     4921 ctttggtgac aaggctgcca aaggcaagtg gactatagct agctttggga gtagattgat
     4981 taactttaga aattcagata aaaatcataa ttgggatact cgagaagttt atccaactaa
     5041 agagttggag aaattgctaa aagattattc tatcgaatat gggcatggcg aatgtatcaa
     5101 agcagctatt tgcggtgaga gcgacaaaaa gttttttgct aagctaacta gtgtcctaaa
     5161 tactatctta caaatgcgta actcaaaaac aggtactgag ttagattatc taatttcacc
     5221 agtagcagat gtaaatggca atttctttga ttcgcgacag gcgccaaaaa atatgcctca
     5281 agatgctgat gccaatggtg cttatcatat tgggctaaaa ggtctgatgc tactaggtag
     5341 gatcaaaaat aatcaagagg gcaaaaaact caatttggtt atcaaaaatg aagagtattt
     5401 tgagttcgtg cagaatagga ataactaatt gagaagagaa aagaaaaccg ccgatcctgt
     5461 ccaccgcatt actgcaaggt agtggacaag accggcggtc ttaagttttt tggctgaaTT
     5521 AATTAAgtac gggttttgct gcccgcaaac gggctgttct ggtgttgcta gtttgttatc
     5581 agaatcgcag atccggcttc agccggtttg ccggctgaaa gcgctatttc ttccagaatt
     5641 gccatgattt tttccccacg ggaggcgtca ctggctcccg tgttgtcggc agctttgatt
     5701 cgataagcag catcgcctgt ttcaggctgt ctatgtgtga ctgttgagct gtaacaagtt
     5761 gtctcaggtg ttcaatttca tgttctagtt gctttgtttt actggtttca cctgttctat
     5821 taggtgttac atgctgttca tctgttacat tgtcgatctg ttcatggtga acagctttga
     5881 atgcaccaaa aactcgtaaa agctctgatg tatctatctt ttttacaccg ttttcatctg
     5941 tgcatatgga cagttttccc tttgatatgt aacggtgaac agttgttcta cttttgtttg
     6001 ttagtcttga tgcttcactg atagatacaa gagccataag aacctcagat ccttccgtat
     6061 ttagccagta tgttctctag tgtggttcgt tgtttttgcg tgagccatga gaacgaacca
     6121 ttgagatcat acttactttg catgtcactc aaaaattttg cctcaaaact ggtgagctga
     6181 atttttgcag ttaaagcatc gtgtagtgtt tttcttagtc cgttatgtag gtaggaatct
     6241 gatgtaatgg ttgttggtat tttgtcacca ttcattttta tctggttgtt ctcaagttcg
     6301 gttacgagat ccatttgtct atctagttca acttggaaaa tcaacgtatc agtcgggcgg
     6361 cctcgcttat caaccaccaa tttcatattg ctgtaagtgt ttaaatcttt acttattggt
     6421 ttcaaaaccc attggttaag ccttttaaac tcatggtagt tattttcaag cattaacatg
     6481 aacttaaatt catcaaggct aatctctata tttgccttgt gagttttctt ttgtgttagt
     6541 tcttttaata accactcata aatcctcata gagtatttgt tttcaaaaga cttaacatgt
     6601 tccagattat attttatgaa tttttttaac tggaaaagat aaggcaatat ctcttcacta
     6661 aaaactaatt ctaatttttc gcttgagaac ttggcatagt ttgtccactg gaaaatctca
     6721 aagcctttaa ccaaaggatt cctgatttcc acagttctcg tcatcagctc tctggttgct
     6781 ttagctaata caccataagc attttcccta ctgatgttca tcatctgagc gtattggtta
     6841 taagtgaacg ataccgtccg ttctttcctt gtagggtttt caatcgtggg gttgagtagt
     6901 gccacacagc ataaaattag cttggtttca tgctccgtta agtcatagcg actaatcgct
     6961 agttcatttg ctttgaaaac aactaattca gacatacatc tcaattggtc taggtgattt
     7021 taatcactat accaattgag atgggctagt caatgataat tactagtcct tttcctttga
     7081 gttgtgggta tctgtaaatt ctgctagacc tttgctggaa aacttgtaaa ttctgctaga
     7141 ccctctgtaa attccgctag acctttgtgt gttttttttg tttatattca agtggttata
     7201 atttatagaa taaagaaaga ataaaaaaag ataaaaagaa tagatcccag ccctgtgtat
     7261 aactcactac tttagtcagt tccgcagtat tacaaaagga tgtcgcaaac gctgtttgct
     7321 cctctacaaa acagacctta aaaccctaaa ggcttaagta gcaccctcgc aagctcgggc
     7381 aaatcgctga atattccttt tgtctccgac catcaggcac ctgagtcgct gtctttttcg
     7441 tgacattcag ttcgctgcgc tcacggctct ggcagtgaat gggggtaaat ggcactacag
     7501 gcgcctttta tggattcatg caaggaaact acccataata caagaaaagc ccgtcacggg
     7561 cttctcaggg cgttttatgg cgggtctgct atgtggtgct atctgacttt ttgctgttca
     7621 gcagttcctg ccctctgatt ttccagtctg accacttcgg attatcccgt gacaggtcat
     7681 tcagactggc taatgcaccc agtaaggcag cggtatcatc aacaggctta cccgtcttac
     7741 tgtccctagt gcttggccta gtatgcgtct cctttacacc tccacaacta gctggtatta
     7801 tgctagcagc tgtcaccgga tgtgctttcc ggtctgatga gtccgtgagg acgaaacagc
     7861 ctctacaaat aattttgttt aactttacaa agaggagaaa ggtaccgcat gagtaaagga
     7921 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg
     7981 cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt
     8041 aaattgattt gcactactgg aaaactacct gttccatggc caacacttgt cactactttg
     8101 ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca tgactttttc
     8161 aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg
     8221 aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag
     8281 ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac
     8341 tataactcac acaatgtata catcacggca gacaaacaaa agaatggaat caaagcgaac
     8401 ttcaaaatta gacacaacat tgaagatgga ggtgttcaac tagcagacca ttatcaacaa
     8461 aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct gtcctaccaa
     8521 tctaagcttt cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta
     8581 acagctgctg ggattacaca tggcatggat gaactataca aataagcttg atatcgaatt
     8641 cctgcagccc gggggatccc attgtacgcg tgctagaggc atcaaataaa acgaaaggct
     8701 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
     8761 aggacaaata ctagagcttg gattctcacc aataaaaaac gcccggcggc aaccgagcgt
     8821 tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag
     8881 cgagctcgat atcaaa
//