LOCUS       pJK528_OSIP_511_(KanR; 12613 bp ds-DNA     circular     23-JUN-2020
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     CDS                9776..10591
                        /label="KanR"
                        /ApEinfo_revcolor=#d6b295
                        /ApEinfo_fwdcolor=#d6b295
     terminator         7516..7543
                        /label="rrnB T2 terminator"
                        /ApEinfo_revcolor=#d6b295
                        /ApEinfo_fwdcolor=#d6b295
     terminator         1105..1199
                        /label="lambda t0 terminator"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
     misc_feature       complement(7030..7049)
                        /label="crRNA_TA2"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
     primer_bind        complement(262..290)
                        /label="GFP-R"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
     CDS                complement(1220..1879)
                        /label="CmR"
                        /ApEinfo_revcolor=#f8d3a9
                        /ApEinfo_fwdcolor=#f8d3a9
     misc_feature       complement(7269..7288)
                        /label="DR_FnCpf1"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
     Terminator         complement(7188..7248)
                        /label="L3S2P21"
                        /ApEinfo_revcolor=#75c6a9
                        /ApEinfo_fwdcolor=#75c6a9
     primer_bind        12012..12030
                        /label="pBRforEco"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     primer_bind        complement(7398..7416)
                        /label="pBRforEco"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
     primer_bind        complement(9849..9868)
                        /label="Kan-R"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
     protein_bind       11112..11159
                        /label="FRT"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
     misc_feature       complement(6823..6842)
                        /label="fnCpf1 direct repeat (Zetsche 2015)"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
     misc_feature       232..948
                        /label="Venus"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     primer_bind        2543..2562
                        /label="Tet-R"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     misc_feature       complement(7289..7323)
                        /label="P_A2"
                        /ApEinfo_revcolor=#f8d3a9
                        /ApEinfo_fwdcolor=#f8d3a9
     misc_feature       complement(6738..7422)
                        /label="from pJK500 via PacI"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
     CDS                8592..9602
                        /label="phage 186 integrase"
                        /ApEinfo_revcolor=#d6b295
                        /ApEinfo_fwdcolor=#d6b295
     misc_feature       complement(7269..7288)
                        /label="fnCpf1 direct repeat (Zetsche 2015)"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
     misc_RNA           133..182
                        /label="sTRSV HHRz"
                        /ApEinfo_revcolor=#d6b295
                        /ApEinfo_fwdcolor=#d6b295
     misc_feature       5493..5495
                        /label="D917A"
                        /ApEinfo_revcolor=#ff9ccd
                        /ApEinfo_fwdcolor=#ff9ccd
     RBS                212..223
                        /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
                        /ApEinfo_revcolor=#9eafd2
                        /ApEinfo_fwdcolor=#9eafd2
     misc_feature       80..85
                        /label="AvrII/SpeI scar"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     terminator         7592..7623
                        /label="tonB terminator"
                        /ApEinfo_revcolor=#75c6a9
                        /ApEinfo_fwdcolor=#75c6a9
     misc_feature       complement(6803..6822)
                        /label="crRNA_TA4"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     primer_bind        complement(11839..11861)
                        /label="pGEX 3'"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
     Terminator         complement(6746..6802)
                        /label="L3S2P55"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
     protein_bind       11172..11386
                        /label="phage 186 attP"
                        /ApEinfo_revcolor=#9eafd2
                        /ApEinfo_fwdcolor=#9eafd2
     promoter           complement(1880..1982)
                        /label="cat promoter"
                        /ApEinfo_revcolor=#9eafd2
                        /ApEinfo_fwdcolor=#9eafd2
     CDS                complement(7740..8453)
                        /label="-lambda repressor (ts)"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
     misc_feature       complement(6886..6966)
                        /label="PlmJ (Lou 2013)"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     promoter           131..205
                        /label="RiboJ"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     misc_feature       complement(6843..6877)
                        /label="P_A1"
                        /ApEinfo_revcolor=#d6b295
                        /ApEinfo_fwdcolor=#d6b295
     protein_bind       2690..2708
                        /label="tet operator"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
     misc_feature       complement(7050..7069)
                        /label="fnCpf1 direct repeat (Zetsche 2015)"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
     misc_feature       7423..7457
                        /label="JKgB17"
                        /ApEinfo_revcolor=#9eafd2
                        /ApEinfo_fwdcolor=#9eafd2
     promoter           2654..2709
                        /label="tetR/tetA promoter s"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
     RBS                8562..8584
                        /label="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)"
                        /ApEinfo_revcolor=#9eafd2
                        /ApEinfo_fwdcolor=#9eafd2
     misc_feature       6618..6737
                        /label="JKgB15"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     misc_feature       2745..6647
                        /label="FndCas12a"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
     misc_feature       complement(6878..7111)
                        /label="JKgB16"
                        /ApEinfo_revcolor=#d6b295
                        /ApEinfo_fwdcolor=#d6b295
     misc_feature       96..130
                        /label="P_A4"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
     primer_bind        complement(9765..9784)
                        /label="pENTR-R"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
     terminator         11503..11749
                        /label="-lambda tL3 terminator"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
     misc_feature       complement(7050..7069)
                        /label="DR_FnCpf1"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
     terminator         complement(7351..7394)
                        /label="bacterial terminator"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
     CDS                complement(2012..2635)
                        /label="TetR"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
     rep_origin         10713..11101
                        /label="R6K gamma ori"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
     primer_bind        885..905
                        /label="GFP-F"
                        /ApEinfo_revcolor=#75c6a9
                        /ApEinfo_fwdcolor=#75c6a9
     protein_bind       7700..7733
                        /label="FRT (minimal)"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
     misc_feature       complement(6973..7029)
                        /label="L3S2P11 (error in SI)"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
     RBS                8542..8550
                        /label="Shine-Dalgarno sequence"
                        /ApEinfo_revcolor=#ff9ccd
                        /ApEinfo_fwdcolor=#ff9ccd
     ribozyme insulator complement(7109..7187)
                        /label="SarJ (Lou 2013)"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
     primer_bind        1794..1813
                        /label="CAT-R"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
     terminator         12071..12157
                        /label="rrnB T1 terminator"
                        /ApEinfo_revcolor=#f8d3a9
                        /ApEinfo_fwdcolor=#f8d3a9
     misc_feature       104..116
                        /label="A4 oper"
                        /ApEinfo_revcolor=#d6b295
                        /ApEinfo_fwdcolor=#d6b295
     misc_feature       76..7461
                        /label="(from pJK511)"
                        /ApEinfo_revcolor=#ff9ccd
                        /ApEinfo_fwdcolor=#ff9ccd
     misc_feature       complement(7249..7268)
                        /label="crRNA_TA1"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
     terminator         1006..1092
                        /label="rrnB T1 terminator"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
     terminator         6648..6737
                        /label="ECK120029600 (Chen 2013)"
                        /ApEinfo_revcolor=#ff9ccd
                        /ApEinfo_fwdcolor=#ff9ccd
     RBS                2727..2738
                        /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     terminator         12254..12340
                        /label="rrnB T1 terminator"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
     terminator         12437..12523
                        /label="rrnB T1 terminator"
                        /ApEinfo_revcolor=#f8d3a9
                        /ApEinfo_fwdcolor=#f8d3a9
     misc_feature       complement(7070..7104)
                        /label="P_A4"
                        /ApEinfo_revcolor=#d6b295
                        /ApEinfo_fwdcolor=#d6b295
     misc_feature       complement(6823..6842)
                        /label="DR_FnCpf1"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
     misc_feature       1093..1098
                        /label="SpeI/XbaI scar"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
     primer             complement(5797..5815)
                        /label="dCpfseqR"
                        /note="sequence: tagacctgcttctctacct"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
     primer             complement(5466..5515)
                        /label="500-Cp-R"
                        /note="sequence: gctaaatgtctttcacctctaGctatacttaatatatgaacatcatttgc"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
     primer             complement(8428..8453)
                        /label="pLPT119-F"
                        /note="sequence: atgagcacaaaaaagaaaccattaac"
                        /ApEinfo_revcolor=#75c6a9
                        /ApEinfo_fwdcolor=#75c6a9
     primer             7415..7441
                        /label="500-vF"
                        /note="sequence: TTAATTAAgtacgggttttgctgcccg"
                        /ApEinfo_revcolor=#ff9ccd
                        /ApEinfo_fwdcolor=#ff9ccd
     primer             complement(6737..6762)
                        /label="502seqR"
                        /note="sequence: gcctcgtgatacgcctattAATTAAt"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
     primer             complement(7442..7461)
                        /label="SC101-R"
                        /note="sequence: acaccagaacagcccgtttg"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
     primer             2739..2766
                        /label="500-Cp-F"
                        /note="sequence: ggatctatgtcaatttatcaagaatttg"
                        /ApEinfo_revcolor=#ff9ccd
                        /ApEinfo_fwdcolor=#ff9ccd
     primer             complement(56..95)
                        /label="528-v-R"
                        /note="sequence: ggagacgcatactaggccaagcgcgcaaatacgcatactg"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
     primer             complement(7304..7323)
                        /label="513-Bss-F"
                        /note="sequence: TATAgcgcgctttacaccaacgggtcacac"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     primer             complement(7393..7414)
                        /label="AHC138-end-F"
                        /note="sequence: taggcgtatcacgaggcagaat"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
     primer             complement(8170..8196)
                        /label="lambda_cI-F"
                        /note="sequence: cgagatgtatgaagcggttagtatgca"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
     primer             6621..6672
                        /label="502-F"
                        /note="sequence: gagttcgtgcagaataggaataactaattgagaagagaaaagaaaaccgccg"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
     primer             6618..6634
                        /label="gB15-F"
                        /note="sequence: tttgagttcgtgcagaa"
                        /ApEinfo_revcolor=#75c6a9
                        /ApEinfo_fwdcolor=#75c6a9
     primer             56..95
                        /label="528-511-F"
                        /note="sequence: cagtatgcgtatttgcgcgcttggcctagtatgcgtctcc"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
     primer             complement(7082..7113)
                        /label="386-BgH-F"
                        /note="sequence: agatctaagctttttacacctccacaactagctgg"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
     primer             complement(7311..7339)
                        /label="385-BglII-F"
                        /note="sequence: agatctcagaaatcattttacaccaacgg"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
     primer             complement(2727..2756)
                        /label="500-vR"
                        /note="sequence: ataaattgacatagatcctttctcctcttt"
                        /ApEinfo_revcolor=#d59687
                        /ApEinfo_fwdcolor=#d59687
     primer             complement(2727..2747)
                        /label="461-chk-R"
                        /note="sequence: ccatagatcctttctcctcttt"
                        /ApEinfo_revcolor=#75c6a9
                        /ApEinfo_fwdcolor=#75c6a9
     primer             7442..7481
                        /label="528-v-F"
                        /note="sequence: caaacgggctgttctggtgtgcatgcctcgagatgcatgg"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
     primer             5490..5515
                        /label="500-Cp-2F"
                        /note="sequence: atagCtagaggtgaaagacatttagc"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
     primer             6973..6994
                        /label="387-Xba-R"
                        /note="sequence: tctagataaaggaccaaaacgaaaaaagacgc"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     primer             6972..6990
                        /label="386-Bam-R"
                        /note="sequence: ggatcctatcggaccaaaacgaaaaaag"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
     primer             complement(7442..7481)
                        /label="528-511-R"
                        /note="sequence: ccatgcatctcgaggcatgcacaccagaacagcccgtttg"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
     primer             complement(1188..1232)
                        /label="378-t0-Rv"
                        /note="sequence: gggcggggcgtaatttgatatcgagctcgcttggactcctgttga"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
     primer             7077..7095
                        /label="gB15-R"
                        /note="sequence: taataccAGCTAGTTGTGG"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
     primer             complement(6621..6646)
                        /label="500-Cp-2R"
                        /note="sequence: tagttattcctattctgcacgaactc"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
     primer             complement(6713..6768)
                        /label="502-R"
                        /note="sequence: aattctgcctcgtgatacgcctattAATTAAttcagccaaaaaacttaagaccgcc"
                        /ApEinfo_revcolor=#f8d3a9
                        /ApEinfo_fwdcolor=#f8d3a9
     primer             complement(7442..7461)
                        /label="119-genomicINT-Rv"
                        /note="sequence: acaccagaacagcccgtttg"
                        /ApEinfo_revcolor=#d59687
                        /ApEinfo_fwdcolor=#d59687
     primer             complement(257..277)
                        /label="VenusR"
                        /note="sequence: caagaattgggacaactccag"
                        /ApEinfo_revcolor=#9eafd2
                        /ApEinfo_fwdcolor=#9eafd2
ORIGIN
        1 gaattcgagc tcggtacccg gggatcctct agagggctta ctaaaagcca gataacagta
       61 tgcgtatttg cgcgcttggc ctagtatgcg tctcctttac acctccacaa ctagctggta
      121 ttatgctagc agctgtcacc ggatgtgctt tccggtctga tgagtccgtg aggacgaaac
      181 agcctctaca aataattttg tttaacttta caaagaggag aaaggtaccg catgagtaaa
      241 ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat
      301 gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc
      361 cttaaattga tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact
      421 ttgggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca gcatgacttt
      481 ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac
      541 gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc
      601 gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac
      661 aactataact cacacaatgt atacatcacg gcagacaaac aaaagaatgg aatcaaagcg
      721 aacttcaaaa ttagacacaa cattgaagat ggaggtgttc aactagcaga ccattatcaa
      781 caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta cctgtcctac
      841 caatctaagc tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt
      901 gtaacagctg ctgggattac acatggcatg gatgaactat acaaataagc ttgatatcga
      961 attcctgcag cccgggggat cccattgtac gcgtgctaga ggcatcaaat aaaacgaaag
     1021 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
     1081 agtaggacaa atactagagc ttggattctc accaataaaa aacgcccggc ggcaaccgag
     1141 cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc
     1201 aagcgagctc gatatcaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt
     1261 cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca
     1321 gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga
     1381 agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg
     1441 ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt
     1501 aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac
     1561 tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac
     1621 tatcccatat caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca
     1681 tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg
     1741 tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg
     1801 actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc
     1861 cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa
     1921 atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgccgat
     1981 caacgtctca ttttcgccag atatcgacgt cttaagaccc actttcacat ttaagttgtt
     2041 tttctaatcc gcatatgatc aattcaaggc cgaataagaa ggctggctct gcaccttggt
     2101 gatcaaataa ttcgatagct tgtcgtaata atggcggcat actatcagta gtaggtgttt
     2161 ccctttcttc tttagcgact tgatgctctt gatcttccaa tacgcaacct aaagtaaaat
     2221 gccccacagc gctgagtgca tataatgcat tctctagtga aaaaccttgt tggcataaaa
     2281 aggctaattg attttcgaga gtttcatact gtttttctgt aggccgtgta cctaaatgta
     2341 cttttgctcc atcgcgatga cttagtaaag cacatctaaa acttttagcg ttattacgta
     2401 aaaaatcttg ccagctttcc ccttctaaag ggcaaaagtg agtatggtgc ctatctaaca
     2461 tctcaatggc taaggcgtcg agcaaagccc gcttattttt tacatgccaa tacaatgtag
     2521 gctgctctac acctagcttc tgggcgagtt tacgggttgt taaaccttcg attccgacct
     2581 cattaagcag ctctaatgcg ctgttaatca ctttactttt atctaatcta gacatcatta
     2641 attcctaatt tttgttgaca ctctatcgtt gatagagtta ttttaccact ccctatcagt
     2701 gatagagaaa agaattcaaa agatctaaag aggagaaagg atctatgtca atttatcaag
     2761 aatttgttaa taaatatagt ttaagtaaaa ctctaagatt tgagttaatc ccacagggta
     2821 aaacacttga aaacataaaa gcaagaggtt tgattttaga tgatgagaaa agagctaaag
     2881 actacaaaaa ggctaaacaa ataattgata aatatcatca gttttttata gaggagatat
     2941 taagttcggt ttgtattagc gaagatttat tacaaaacta ttctgatgtt tattttaaac
     3001 ttaaaaagag tgatgatgat aatctacaaa aagattttaa aagtgcaaaa gatacgataa
     3061 agaaacaaat atctgaatat ataaaggact cagagaaatt taagaatttg tttaatcaaa
     3121 accttatcga tgctaaaaaa gggcaagagt cagatttaat tctatggcta aagcaatcta
     3181 aggataatgg tatagaacta tttaaagcca atagtgatat cacagatata gatgaggcgt
     3241 tagaaataat caaatctttt aaaggttgga caacttattt taagggtttt catgaaaata
     3301 gaaaaaatgt ttatagtagc aatgatattc ctacatctat tatttatagg atagtagatg
     3361 ataatttgcc taaatttcta gaaaataaag ctaagtatga gagtttaaaa gacaaagctc
     3421 cagaagctat aaactatgaa caaattaaaa aagatttggc agaagagcta acctttgata
     3481 ttgactacaa aacatctgaa gttaatcaaa gagttttttc acttgatgaa gtttttgaga
     3541 tagcaaactt taataattat ctaaatcaaa gtggtattac taaatttaat actattattg
     3601 gtggtaaatt tgtaaatggt gaaaatacaa agagaaaagg tataaatgaa tatataaatc
     3661 tatactcaca gcaaataaat gataaaacac tcaaaaaata taaaatgagt gttttattta
     3721 agcaaatttt aagtgataca gaatctaaat cttttgtaat tgataagtta gaagatgata
     3781 gtgatgtagt tacaacgatg caaagttttt atgagcaaat agcagctttt aaaacagtag
     3841 aagaaaaatc tattaaagaa acactatctt tattatttga tgatttaaaa gctcaaaaac
     3901 ttgatttgag taaaatttat tttaaaaatg ataaatctct tactgatcta tcacaacaag
     3961 tttttgatga ttatagtgtt attggtacag cggtactaga atatataact caacaaatag
     4021 cacctaaaaa tcttgataac cctagtaaga aagagcaaga attaatagcc aaaaaaactg
     4081 aaaaagcaaa atacttatct ctagaaacta taaagcttgc cttagaagaa tttaataagc
     4141 atagagatat agataaacag tgtaggtttg aagaaatact tgcaaacttt gcggctattc
     4201 cgatgatatt tgatgaaata gctcaaaaca aagacaattt ggcacagata tctatcaaat
     4261 atcaaaatca aggtaaaaaa gacctacttc aagctagtgc ggaagatgat gttaaagcta
     4321 tcaaggatct tttagatcaa actaataatc tcttacataa actaaaaata tttcatatta
     4381 gtcagtcaga agataaggca aatattttag acaaggatga gcatttttat ctagtatttg
     4441 aggagtgcta ctttgagcta gcgaatatag tgcctcttta taacaaaatt agaaactata
     4501 taactcaaaa gccatatagt gatgagaaat ttaagctcaa ttttgagaac tcgactttgg
     4561 ctaatggttg ggataaaaat aaagagcctg acaatacggc aattttattt atcaaagatg
     4621 ataaatatta tctgggtgtg atgaataaga aaaataacaa aatatttgat gataaagcta
     4681 tcaaagaaaa taaaggcgag ggttataaaa aaattgttta taaactttta cctggcgcaa
     4741 ataaaatgtt acctaaggtt ttcttttctg ctaaatctat aaaattttat aatcctagtg
     4801 aagatatact tagaataaga aatcattcca cacatacaaa aaatggtagt cctcaaaaag
     4861 gatatgaaaa atttgagttt aatattgaag attgccgaaa atttatagat ttttataaac
     4921 agtctataag taagcatccg gagtggaaag attttggatt tagattttct gatactcaaa
     4981 gatataattc tatagatgaa ttttatagag aagttgaaaa tcaaggctac aaactaactt
     5041 ttgaaaatat atcagagagc tatattgata gcgtagttaa tcagggtaaa ttgtacctat
     5101 tccaaatcta taataaagat ttttcagctt atagcaaagg gcgaccaaat ctacatactt
     5161 tatattggaa agcgctgttt gatgagagaa atcttcaaga tgtggtttat aagctaaatg
     5221 gtgaggcaga gcttttttat cgtaaacaat caatacctaa aaaaatcact cacccagcta
     5281 aagaggcaat agctaataaa aacaaagata atcctaaaaa agagagtgtt tttgaatatg
     5341 atttaatcaa agataaacgc tttactgaag ataagttttt ctttcactgt cctattacaa
     5401 tcaattttaa atctagtgga gctaataagt ttaatgatga aatcaattta ttgctaaaag
     5461 aaaaagcaaa tgatgttcat atattaagta tagCtagagg tgaaagacat ttagcttact
     5521 atactttggt agatggtaaa ggcaatatca tcaaacaaga tactttcaac atcattggta
     5581 atgatagaat gaaaacaaac taccatgata agcttgctgc aatagagaaa gatagggatt
     5641 cagctaggaa agactggaaa aagataaata acatcaaaga gatgaaagag ggctatctat
     5701 ctcaggtagt tcatgaaata gctaagctag ttatagagta taatgctatt gtggtttttg
     5761 aggatttaaa ttttggattt aaaagagggc gtttcaaggt agagaagcag gtctatcaaa
     5821 agttagaaaa aatgctaatt gagaaactaa actatctagt tttcaaagat aatgagtttg
     5881 ataaaactgg gggagtgctt agagcttatc agctaacagc accttttgag acttttaaaa
     5941 agatgggtaa acaaacaggt attatctact atgtaccagc tggttttact tcaaaaattt
     6001 gtcctgtaac tggttttgta aatcagttat atcctaagta tgaaagtgtc agcaaatctc
     6061 aagagttctt tagtaagttt gacaagattt gttataacct tgataagggc tattttgagt
     6121 ttagttttga ttataaaaac tttggtgaca aggctgccaa aggcaagtgg actatagcta
     6181 gctttgggag tagattgatt aactttagaa attcagataa aaatcataat tgggatactc
     6241 gagaagttta tccaactaaa gagttggaga aattgctaaa agattattct atcgaatatg
     6301 ggcatggcga atgtatcaaa gcagctattt gcggtgagag cgacaaaaag ttttttgcta
     6361 agctaactag tgtcctaaat actatcttac aaatgcgtaa ctcaaaaaca ggtactgagt
     6421 tagattatct aatttcacca gtagcagatg taaatggcaa tttctttgat tcgcgacagg
     6481 cgccaaaaaa tatgcctcaa gatgctgatg ccaatggtgc ttatcatatt gggctaaaag
     6541 gtctgatgct actaggtagg atcaaaaata atcaagaggg caaaaaactc aatttggtta
     6601 tcaaaaatga agagtatttt gagttcgtgc agaataggaa taactaattg agaagagaaa
     6661 agaaaaccgc cgatcctgtc caccgcatta ctgcaaggta gtggacaaga ccggcggtct
     6721 taagtttttt ggctgaaTTA ATTAAggacc aaaacgaaaa aagacgcttt tcagcgtctt
     6781 attgttcgtc tttggtaccg agaccAGCTA GTTGTGGAgg tgATCTACAA CAGTAGAAAT
     6841 TAgctagcat aataccTAGG ACTGAGCTAg gtgtaaaatc ctatcTTAAA CAAAATTATT
     6901 TGTAGAGGTC ATAAGTTTCG TCGCATTTCA GCGACTCATC AGTGGGCTTA GCCCAGACTT
     6961 ATGACTggat ccggaccaaa acgaaaaaag acgctcgaaa gcgtctcttt tctggaattt
     7021 ggtaccgaga ccCGTGTGAC CCGTTggtgA TCTACAACAG TAGAAATTAg ctagcataat
     7081 accAGCTAGT TGTGGAggtg taaaaagcTT AAACAAAATT ATTTGTAGAG GACTGTTTCG
     7141 GCCCTTTTGG GCCATCGTCA GGTCGGATAC ACATCCGGCG ACAGTCTgga ccaaaacgaa
     7201 aaaaggcccc cctttcggga ggcctctttt ctggaatttg gtaccgagac cTAGGACTGA
     7261 GCTAggtgAT CTACAACAGT AGAAATTAgc tagcataata ccCGTGTGAC CCGTTggtgt
     7321 aaaatgattt ctgagatctt tagaattcca gaaatcatcc ttagcgaaag ctaaggattt
     7381 tttttatctg aaattctgcc tcgtgatacg cctattAATT AAgtacgggt tttgctgccc
     7441 gcaaacgggc tgttctggtg tgcatgcctc gagatgcatg gcgcctaacc taaactgaca
     7501 ggcatcaaat taagcagaag gccatcctga cggatggcct ttttgcgttt cgaacaattg
     7561 aaaaaacctc gcgccttacc tgttgagtaa tagtcaaaag cctccggtcg gaggcttttg
     7621 actttctgct tactgaattt cggtggtgcc gttaattaac cggtgggccc tcatgataat
     7681 aatggtttct tagacgtccg aagttcctat tctctagaaa gtataggaac ttccctaggt
     7741 cagccaaacg tctcttcagg ccactgacta gcgataactt tccccacaac ggaacaactc
     7801 tcattgcatg ggatcattgg gtactgtggg tttagtggtt gtaaaaacac ctgaccgcta
     7861 tccctgatca gtttcttgaa ggtaaactca tcacccccaa gtctggctat gcagaaatca
     7921 cctggctcaa cagcctgctc agggtcaacg agaattaaca ttccgtcagg aaagcttggc
     7981 ttggagcctg ttggtgcggt catggaatta ccttcaacct caagccagaa tgcagaatca
     8041 ctggcttttt tggttgtgct tacccatctc tccgcatcac ctttggtaaa ggttctaagc
     8101 ttaggtgaga acatccctgc ctgaacatga gaaaaaacag ggtactcata ctcacttcta
     8161 agtgacggct gcatactaac cgcttcatac atctcgtaga tttctctggc gattgaaggg
     8221 ctaaattctt caacgctaac tttgagaatt tttgtaagca atgcggcgtt ataagcattt
     8281 aatgcattga tgccattaaa taaagcacca acgcctgact gccccatccc catcttgtct
     8341 gcgacagatt cctgggataa gccaagttca tttttctttt tttcataaat tgctttaagg
     8401 cgacgtgcgt cctcaagctg ctcttgtgtt aatggtttct tttttgtgct catacgttaa
     8461 atctatcacc gcaagggata aatatctaac accgtgcgtg ttgactattt tacctctggc
     8521 ggtgataatg gttgcatgta ctaaggaggt tgtatacgcg ttttgtttaa ctttaagaag
     8581 gagatataca tatgaccgtc cgtaaaaatc cggctggcgg ttggatttgt gaactctacc
     8641 caaacggtgc aaaaggcaaa cgtatcagaa agaaattcgc tactaaaggc gaggctctgg
     8701 cgtttgagca gtacaccgtt caaaacccgt ggcaggaaga aaaggaagac aggcgcacgt
     8761 taaaagagct ggttgattca tggtatagcg ctcatggcat tacactgaaa gatggtttga
     8821 aacgccagtt agccatgcac catgcttttg agtgtatggg cgaaccactc gcacgcgatt
     8881 tcgatgcgca gatgttttcc cgctaccgag aaaaacggtt aaaaggtgag tatgcccgtt
     8941 caaacagagt gaaagaggta tcgcctcgca cgcttaatct tgagctggcc tacttccggg
     9001 cggtgttcaa tgagctaaac cgcctcggag aatggaaggg tgaaaaccca ctaaaaaata
     9061 tgcgcccatt ccgcacagaa gaaatggaaa tgacttggct aactcacgac caaatttcgc
     9121 aactgctcgg agagtgtaac cgacatgacc accctgattt agaaaccgtg gtaagaatct
     9181 gtctcgcaac tggcgcacgg tggtctgagg ccgagagtct gagaaaaagc caactcgcga
     9241 aatacaaaat cacatacacc aacacgaaag gtagaaaaaa tcgcaccgtc ccaatcagca
     9301 aagagctcta tgagtctctg cctgatgata aaaagggccg gttgttcagt gattgttatg
     9361 gcgcgttccg gtcagctttg gaaagaacag gtatcgaact accggcagga caacttaccc
     9421 acgttttacg tcacaccttc gctagtcact ttatgatgaa tggcggtaat attttggtgt
     9481 tgcagcgcgt actcggccat acagatataa aaatgaccat gcgatatgcg cactttgcac
     9541 ccgaccattt agaggatgca gttaaactaa atcctttagt tcacattact aacagcaaat
     9601 aagttatgga ggttgtatgt cagttcggct agcccatggg tatggacagt tttccctttg
     9661 atatgtaacg cacgttgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat
     9721 atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag
     9781 ccatattcaa cgggaaacgt cttgctcccg tccgcgctta aactccaaca tggacgctga
     9841 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg
     9901 cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc
     9961 caatgatgtt acagatgaga tggtccgtct caactggctg acggagttta tgcctctccc
    10021 gaccatcaag cattttatcc gtactcctga tgatgcgtgg ttactcacca ccgcgattcc
    10081 tgggaaaaca gccttccagg tattagaaga atatcctgat tcaggtgaaa atattgttga
    10141 tgcgctggcc gtgttcctgc gccggttaca ttcgattcct gtttgtaatt gtccttttaa
    10201 cagcgatcgt gtatttcgtc ttgctcaggc gcaatcacgc atgaataacg gtttggttga
    10261 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat
    10321 gcacaagctc ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga
    10381 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gggtcggaat
    10441 cgcagaccgt taccaggacc ttgccattct ttggaactgc ctcggtgagt tttctccttc
    10501 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca
    10561 gtttcatttg atgctcgatg agtttttcta ataatactag cagaaatcat ccttagcgaa
    10621 agctaaggat tttttttatc tgattaccgc ctttgagtga gcgtcgacct agtgcggccg
    10681 caagatccgg ccacgatgcg tccggcgtag aggatctgaa gatcagcagt tcaacctgtt
    10741 gatagtacgt actaagctct catgtttcac gtactaagct ctcatgttta acgtactaag
    10801 ctctcatgtt taacgaacta aaccctcatg gctaacgtac taagctctca tggctaacgt
    10861 actaagctct catgtttcac gtactaagct ctcatgtttg aacaataaaa ttaatataaa
    10921 tcagcaactt aaatagcctc taaggtttta agttttataa gaaaaaaaag aatatataag
    10981 gcttttaaag cttttaaggt ttaacggttg tggacaacaa gccagggatg taacgcactg
    11041 agaagccctt agagcctctc aaagcaattt tgagtgacac aggaacactt aacggctgac
    11101 atgggaatta ggaagttcct attccgaagt tcctattctc tagaaagtat aggaacttcc
    11161 atatgccatg ggcgatggtt ctgagtaaca gataatagaa tggcgataaa gtggcggtag
    11221 aaatggcgaa taataggtaa ttattggcaa acaatggcaa tctatgtcaa tgataaataa
    11281 cgcatactat tgattttcgg ttgttcttgt aggaactcat aatcgcttgg tcgctggttc
    11341 aagtccagca ggggccacca aatttaagta gtaaatacat acagttaggc cactctctcg
    11401 agtggccttt ttgttgcctc gttttcgagt ggcgatagaa tggcggtgga ttttttaccg
    11461 ccactttcct gaggagtgca tagctagctt cttcgtctgt ttctactggt attggcacaa
    11521 acctgattcc aatttgagca aggctatgtg ccatctcgat actcgttctt aactcaacag
    11581 aagatgcttt gtgcatacag cccctcgttt attatttatc tcctcagcca gccgctgtgc
    11641 tttcagtgga tttcggataa cagaaaggcc gggaaatacc cagcctcgct ttgtaacgga
    11701 gtagagacga aagtgattgc gcctacccgg atattatcgt gaggatgcgt catcgccatt
    11761 aattcactga tcagtgatag ctgtcaaaca tgagaattga tccggctgcc tcgcgcgttt
    11821 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
    11881 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
    11941 tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatgctg cacatgacat
    12001 taacctataa aaataggcgt atcacgaggc cctttcgtct tcaagaatta attcccaatt
    12061 ccccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
    12121 ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg atttgaacgt
    12181 tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg ccaggaatta
    12241 attccccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
    12301 ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa
    12361 cgttgcgaag caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggaa
    12421 ttaattcccc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
    12481 tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt
    12541 gaacgttgcg aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag
    12601 gaattgggga tcg
//