LOCUS       Exported                6652 bp ds-DNA     circular SYN 24-JUN-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pAAV.SV40.Thbs1-Delta-TSR1-HA.SV40(polyA)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6652)
  AUTHORS   UNKNOWN
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jun 24, 2020 from SnapGene Viewer 5.1.3.1
            https://www.snapgene.com
COMMENT     ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..6652
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /label=AAV2\ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2
                     vntifkey=34"
                     /note="color: #ffff99"
     enhancer        168..296
                     /label=partial CMV enhancer
                     /label=partial\CMV\enhancer
                     /note="vntifkey=9"
                     /note="color: #c0c0c0"
     promoter        297..500
                     /label=CMV promoter
                     /label=CMV\promoter
                     /note="human cytomegalovirus (CMV) immediate early promoter
                     vntifkey=30"
                     /note="color: #c0c0c0; direction: RIGHT"
     intron          542..634
                     /label=SV40 intron
                     /label=SV40\intron
                     /note="vntifkey=15"
                     /note="color: #------"
     CDS             685..3699
                     /codon_start=1
                     /label=Thbs1-Delta-TSR1-C-HA
                     /label=Thbs1-delta-TSR-C-HA
                     /note="vntifkey=4"
                     /note="color: #ff9900"
                     /translation="MELLRGLGVLFLLHMCGSNRIPESGGDNGVFDIFELIGGARRGPG
                     RRLVKGQDLSSPAFRIENANLIPAVPDDKFQDLLDAVWADKGFIFLASLRQMKKTRGTL
                     LAVERKDNTGQIFSVVSNGKAGTLDLSLSLPGKQQVVSVEEALLATGQWKSITLFVQED
                     RAQLYIDCDKMESAELDVPIQSIFTRDLASVARLRVAKGDVNDNFQGVLQNVRFVFGTT
                     PEDILRNKGCSSSATNVLLTLDNNVVNGSSPAIRTNYIGHKTKDLQAICGLSCDELSSM
                     VLELKGLRTIVTTLQDSIRKVTEENRELVSELKRPPLCFHNGVQYKNNEEWTVDSCTEC
                     HCQNSVTICKKVSCPIMPCSNATVPDGECCPRCWPSDSADDGIDGCLSNPCFAGAKCTS
                     YPDGSWKCGACPPGYSGNGIQCKDVDECKEVPDACFNHNGEHRCKNTDPGYNCLPCPPR
                     FTGSQPFGRGVEHAMANKQVCKPRNPCTDGTHDCNKNAKCNYLGHYSDPMYRCECKPGY
                     AGNGIICGEDTDLDGWPNENLVCVANATYHCKKDNCPNLPNSGQEDYDKDGIGDACDDD
                     DDNDKIPDDRDNCPFHYNPAQYDYDRDDVGDRCDNCPYNHNPDQADTDKNGEGDACAVD
                     IDGDGILNERDNCQYVYNVDQRDTDMDGVGDQCDNCPLEHNPDQLDSDSDLIGDTCDNN
                     QDIDEDGHQNNLDNCPYVPNANQADHDKDGKGDACDHDDDNDGIPDDRDNCRLVPNPDQ
                     KDSDGDGRGDACKDDFDHDNVPDIDDICPENFDISETDFRQFQMIPLDPKGTSQNDPNW
                     VVRHQGKELVQTVNCDPGLAVGYDEFNAVDFSGTFFINTERDDDYAGFVFGYQSSSRFY
                     VVMWKQVTQSYWDTNPTRAQGYSGLSVKVVNSTTGPGEHLRNALWHTGNTPGQVRTLWH
                     DPRHIGWKDFTAYRWRLSHRPKTGYIRVVMYEGKKIMADSGPIYDKTYAGGRLGLFVFS
                     QEMVFFSDMKYECRDS"
     CDS             3700..3726
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="vntifkey=4"
                     /note="color: #ff0000"
                     /translation="YPYDVPDYA"
     intron          3770..3903
                     /label=SV40 polyA
                     /label=SV40\polyA
                     /note="vntifkey=15"
                     /note="color: #------"
     repeat_region   3915..4055
                     /label=AAV2 ITR
                     /label=AAV2\ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2
                     vntifkey=34"
                     /note="color: #ffff99"
     rep_origin      4130..4585
                     /direction=RIGHT
                     /label=f1 ori
                     /label=f1\ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis
                     vntifkey=33"
                     /note="color: #------"
     promoter        4867..4971
                     /gene="bla"
                     /label=AmpR promoter
                     /label=AmpR\promoter
                     /note="vntifkey=30"
                     /note="color: #800000; direction: RIGHT"
     CDS             4972..5832
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics
                     vntifkey=4"
                     /note="color: #ff9966"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6003..6591
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication
                     vntifkey=33"
                     /note="color: #------"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgcc gctcgtgacc taggcatatg ccaagtacgc
      181 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      241 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      301 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      361 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      421 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      481 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccgg
      541 actctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg
      601 tttctctctt ttagattcca acctttggaa ctgaattccg cgggcccggg atcctctaga
      661 gtcgacctgc agaagcttat gaccatggag ctcctgcggg gactaggtgt cctgttcctg
      721 ttgcatatgt gtggaagcaa ccgcattcca gagtctgggg gagataacgg tgtgtttgac
      781 atctttgaac tcattggagg tgcacgaagg ggccccggtc gccgactggt gaagggccaa
      841 gatctatcca gccccgcctt ccgcattgag aatgccaacc tgatccccgc tgtgccggat
      901 gacaagttcc aagacctact ggacgctgtg tgggccgaca aaggcttcat cttcctggct
      961 tccttgaggc agatgaagaa gacccggggc acactcctgg ctgtggaacg gaaagacaac
     1021 actggccaga tcttcagtgt ggtctccaac ggcaaagctg gcaccctgga cctgagcctg
     1081 agcctgccag ggaagcaaca agtggtgtca gtggaggaag ctctcctggc cactggccag
     1141 tggaagagca tcacgctgtt tgttcaagag gaccgggctc aactctacat agactgtgat
     1201 aagatggaga gcgcggagct ggatgtaccc atccagagca tcttcaccag ggatctggcc
     1261 agcgttgcca ggctccgagt tgcaaaggga gatgtcaatg acaattttca gggggtgctg
     1321 cagaatgtga ggtttgtctt tggaaccacc ccagaagaca ttctcaggaa caaaggctgc
     1381 tccagctcag ctaccaacgt ccttcttacc cttgacaaca acgtggtgaa cggttccagc
     1441 cctgctatcc gcaccaacta catcggccac aaaacaaagg acctccaagc tatctgtggc
     1501 ctctcctgtg atgaactatc cagcatggtc ctggaactga agggcctgcg caccatcgtg
     1561 accactctgc aggacagcat ccgaaaagtg acggaagaga acagagagct ggtcagtgag
     1621 ctgaagcggc ctcccctctg ctttcacaat ggagtccagt acaagaacaa cgaggagtgg
     1681 actgtagaca gttgcacaga gtgtcactgc cagaactcgg ttaccatctg caaaaaggtg
     1741 tcctgtccca tcatgccctg ctccaacgcc acagttcctg atggtgaatg ctgcccacgg
     1801 tgctggccca gcgactctgc tgacgatggc attgatggat gcctgtccaa tccctgcttt
     1861 gctggtgcca agtgtactag ctaccctgat ggtagctgga aatgtggtgc gtgtcctcct
     1921 ggctacagtg gaaatggcat ccagtgcaaa gacgtcgatg agtgcaaaga agtgcctgat
     1981 gcttgcttca atcacaacgg agaacatcgg tgcaagaaca cagatcctgg ctacaactgc
     2041 ctgccctgcc caccacgatt cactggctca cagcccttcg gccgaggtgt cgaacatgcc
     2101 atggccaaca aacaggtgtg caaaccgcga aacccctgca cggacgggac gcatgactgc
     2161 aacaagaacg ctaagtgcaa ctacctgggt cactacagtg accccatgta ccgctgtgag
     2221 tgcaagcccg gctatgcagg caatggcatc atctgcggag aggacacaga cctggacggc
     2281 tggcctaatg aaaacctggt gtgtgtggcc aacgcaacct accactgcaa aaaggacaac
     2341 tgccccaacc ttcccaactc ggggcaggaa gactatgaca aggacgggat tggcgatgcc
     2401 tgcgatgatg acgatgacaa cgacaagatc cccgatgaca gggacaactg tccattccat
     2461 tacaacccag cccagtatga ctatgacaga gatgatgtgg gagaccgctg tgacaactgc
     2521 ccctacaacc acaaccctga ccaagcagac acagacaaaa acggggaggg cgatgcctgt
     2581 gctgtggaca tcgatggaga tggaatcctc aatgaacgag acaactgcca gtacgtttac
     2641 aacgtggacc agagggacac ggacatggat ggggttggag atcagtgtga caactgcccc
     2701 ctggaacaca atccagacca gctggactct gactcagacc tcatagggga cacttgtgac
     2761 aacaatcagg acatcgatga ggatggccat cagaacaatc tggacaactg tccctatgtg
     2821 cctaatgcca accaggccga ccatgataaa gatggcaaag gagatgcctg tgaccatgac
     2881 gatgacaatg acggcatccc tgatgacaga gacaactgca ggctggtgcc caatcctgac
     2941 cagaaggact ctgatggtga tggccgaggt gacgcctgca aagacgactt tgaccatgac
     3001 aatgtgccag atattgatga catctgtcct gagaattttg acatcagtga aaccgatttc
     3061 cgacaattcc agatgattcc tctagatccc aaaggaacct cccaaaatga ccctaactgg
     3121 gttgtccgcc atcagggcaa agaacttgtc cagactgtaa actgtgaccc tggacttgct
     3181 gtaggttatg atgagtttaa tgctgtggac ttcagcggta ccttcttcat caacaccgag
     3241 agagatgatg actacgctgg ctttgttttc ggctaccagt ccagcagccg cttctacgtt
     3301 gtgatgtgga aacaagtcac ccagtcctac tgggacacca accccacaag ggctcaggga
     3361 tactcaggcc tgtctgtaaa ggttgtgaac tccaccaccg gccctggcga gcacctgcgg
     3421 aatgcactgt ggcacacagg aaacacccct ggccaggtgc gcaccctgtg gcatgaccct
     3481 cgccacatcg gctggaaaga tttcactgca tacagatggc gtctcagcca caggccaaag
     3541 accggttata tcagagtggt gatgtatgaa ggaaagaaaa tcatggctga ctcgggaccc
     3601 atctatgaca aaacctacgc cggcggtaga ctaggcctgt tcgtcttctc tcaggaaatg
     3661 gtgttcttct cagacatgaa atacgagtgt cgagattcct acccatacga tgttccagat
     3721 tacgcttaac tcgagagatc taggccgcta ggcctcacct gcgatctcga tgctttattt
     3781 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     3841 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
     3901 aaactagcgg ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg
     3961 ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc
     4021 ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt attttctcct
     4081 tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt
     4141 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     4201 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     4261 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     4321 cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga
     4381 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     4441 caaactggaa caacactcaa ccctatctcg ggctattctt ttgatttata agggattttg
     4501 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     4561 aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc
     4621 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     4681 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     4741 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     4801 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     4861 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     4921 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     4981 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     5041 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     5101 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     5161 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     5221 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     5281 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     5341 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     5401 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     5461 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     5521 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     5581 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     5641 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     5701 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     5761 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     5821 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     5881 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     5941 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     6001 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     6061 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     6121 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     6181 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     6241 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     6301 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     6361 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     6421 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     6481 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     6541 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     6601 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gt
//
