LOCUS       Exported                6498 bp ds-DNA     circular SYN 24-JUN-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pAAV.CMV-SV40.THBS1-Delta-CTD-HA.SV40(polyA)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6498)
  AUTHORS   UNKNOWN
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jun 24, 2020 from SnapGene Viewer 5.1.3.1
            https://www.snapgene.com
COMMENT     ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..6498
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /label=AAV2\ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2
                     vntifkey=34"
                     /note="color: #ffff99"
     enhancer        168..296
                     /label=partial CMV enhancer
                     /label=partial\CMV\enhancer
                     /note="vntifkey=9"
                     /note="color: #c0c0c0; direction: RIGHT"
     promoter        297..500
                     /label=CMV promoter
                     /label=CMV\promoter
                     /note="human cytomegalovirus (CMV) immediate early promoter
                     vntifkey=30"
                     /note="color: #c0c0c0; direction: RIGHT"
     intron          542..634
                     /label=SV40 intron
                     /label=SV40\intron
                     /note="vntifkey=15"
                     /note="color: #------"
     CDS             678..3539
                     /codon_start=1
                     /label=THBS1-Delta-CTD
                     /label=Thbs1-C-HA-T3-8
                     /note="vntifkey=4"
                     /note="color: #ff9900"
                     /translation="MELLRGLGVLFLLHMCGSNRIPESGGDNGVFDIFELIGGARRGPG
                     RRLVKGQDLSSPAFRIENANLIPAVPDDKFQDLLDAVWADKGFIFLASLRQMKKTRGTL
                     LAVERKDNTGQIFSVVSNGKAGTLDLSLSLPGKQQVVSVEEALLATGQWKSITLFVQED
                     RAQLYIDCDKMESAELDVPIQSIFTRDLASVARLRVAKGDVNDNFQGVLQNVRFVFGTT
                     PEDILRNKGCSSSATNVLLTLDNNVVNGSSPAIRTNYIGHKTKDLQAICGLSCDELSSM
                     VLELKGLRTIVTTLQDSIRKVTEENRELVSELKRPPLCFHNGVQYKNNEEWTVDSCTEC
                     HCQNSVTICKKVSCPIMPCSNATVPDGECCPRCWPSDSADDGWSPWSEWTSCSATCGNG
                     IQQRGRSCDSLNNRCEGSSVQTRTCHIQECDKRFKQDGGWSHWSPWSSCSVTCGDGVIT
                     RIRLCNSPSPQMNGKPCEGEARETKACKKDACPINGGWGPWSPWDICSVTCGGGVQRRS
                     RLCNNPTPQFGGKDCVGDVTENQVCNKQDCPIDGCLSNPCFAGAKCTSYPDGSWKCGAC
                     PPGYSGNGIQCKDVDECKEVPDACFNHNGEHRCKNTDPGYNCLPCPPRFTGSQPFGRGV
                     EHAMANKQVCKPRNPCTDGTHDCNKNAKCNYLGHYSDPMYRCECKPGYAGNGIICGEDT
                     DLDGWPNENLVCVANATYHCKKDNCPNLPNSGQEDYDKDGIGDACDDDDDNDKIPDDRD
                     NCPFHYNPAQYDYDRDDVGDRCDNCPYNHNPDQADTDKNGEGDACAVDIDGDGILNERD
                     NCQYVYNVDQRDTDMDGVGDQCDNCPLEHNPDQLDSDSDLIGDTCDNNQDIDEDGHQNN
                     LDNCPYVPNANQADHDKDGKGDACDHDDDNDGIPDDRDNCRLVPNPDQKDSDGDGRGDA
                     CKDDFDHDNVPDIDDICPENFDIS"
     CDS             3546..3572
                     /codon_start=1
                     /label=HA-tag
                     /label=C-HA-Tag
                     /label=insertion_seq
                     /note="vntifkey=14"
                     /note="color: #ff0000"
                     /translation="YPYDVPDYA"
     intron          3616..3749
                     /label=SV40 polyA
                     /label=SV40\polyA
                     /note="vntifkey=15"
                     /note="color: #------"
     repeat_region   3761..3901
                     /label=AAV2 ITR
                     /label=AAV2\ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2
                     vntifkey=34"
                     /note="color: #ffff99"
     rep_origin      3976..4431
                     /direction=RIGHT
                     /label=f1 ori
                     /label=f1\ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis
                     vntifkey=33"
                     /note="color: #------"
     promoter        4713..4817
                     /gene="bla"
                     /label=AmpR promoter
                     /label=AmpR\promoter
                     /note="vntifkey=30"
                     /note="color: #800000; direction: RIGHT"
     CDS             4818..5678
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics
                     vntifkey=4"
                     /note="color: #ff9966"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5849..6437
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication
                     vntifkey=33"
                     /note="color: #------"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgcc gctcgtgacc taggcatatg ccaagtacgc
      181 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      241 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      301 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      361 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      421 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      481 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccgg
      541 actctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg
      601 tttctctctt ttagattcca acctttggaa ctgaattccg cgggcccggg atcctctaga
      661 gtcgacctgc agaagctatg gagctcctgc ggggactagg tgtcctgttc ctgttgcata
      721 tgtgtggaag caaccgcatt ccagagtctg ggggagataa cggtgtgttt gacatctttg
      781 aactcattgg aggtgcacga aggggccccg gtcgccgact ggtgaagggc caagatctat
      841 ccagccccgc cttccgcatt gagaatgcca acctgatccc cgctgtgccg gatgacaagt
      901 tccaagacct actggacgct gtgtgggccg acaaaggctt catcttcctg gcttccttga
      961 ggcagatgaa gaagacccgg ggcacactcc tggctgtgga acggaaagac aacactggcc
     1021 agatcttcag tgtggtctcc aacggcaaag ctggcaccct ggacctgagc ctgagcctgc
     1081 cagggaagca acaagtggtg tcagtggagg aagctctcct ggccactggc cagtggaaga
     1141 gcatcacgct gtttgttcaa gaggaccggg ctcaactcta catagactgt gataagatgg
     1201 agagcgcgga gctggatgta cccatccaga gcatcttcac cagggatctg gccagcgttg
     1261 ccaggctccg agttgcaaag ggagatgtca atgacaattt tcagggggtg ctgcagaatg
     1321 tgaggtttgt ctttggaacc accccagaag acattctcag gaacaaaggc tgctccagct
     1381 cagctaccaa cgtccttctt acccttgaca acaacgtggt gaacggttcc agccctgcta
     1441 tccgcaccaa ctacatcggc cacaaaacaa aggacctcca agctatctgt ggcctctcct
     1501 gtgatgaact atccagcatg gtcctggaac tgaagggcct gcgcaccatc gtgaccactc
     1561 tgcaggacag catccgaaaa gtgacggaag agaacagaga gctggtcagt gagctgaagc
     1621 ggcctcccct ctgctttcac aatggagtcc agtacaagaa caacgaggag tggactgtag
     1681 acagttgcac agagtgtcac tgccagaact cggttaccat ctgcaaaaag gtgtcctgtc
     1741 ccatcatgcc ctgctccaac gccacagttc ctgatggtga atgctgccca cggtgctggc
     1801 ccagcgactc tgctgacgat ggctggtctc cctggtctga gtggacctcc tgctctgcca
     1861 catgtggcaa tggaattcag caacgtggtc gttcctgtga cagcctcaac aacagatgcg
     1921 agggctcttc ggtacagaca aggacctgcc acattcagga gtgtgacaaa agatttaaac
     1981 aggatggtgg ctggagtcac tggtctccat ggtcgtcctg ttctgtgacc tgtggtgacg
     2041 gtgtgatcac aaggatccgg ctctgcaact cccccagccc ccagatgaac gggaagccct
     2101 gtgaaggtga agcccgggag accaaagcct gcaagaaaga cgcctgccca attaatggag
     2161 gctggggtcc ctggtcacca tgggacatct gctctgtcac ctgtggagga ggagtgcaga
     2221 gacgcagccg actctgtaac aaccccacac cccagtttgg aggcaaagac tgtgttggcg
     2281 atgtgacaga aaatcaagtt tgcaacaagc aggactgccc aattgatgga tgcctgtcca
     2341 atccctgctt tgctggtgcc aagtgtacta gctaccctga tggtagctgg aaatgtggtg
     2401 cgtgtcctcc tggctacagt ggaaatggca tccagtgcaa agacgtcgat gagtgcaaag
     2461 aagtgcctga tgcttgcttc aatcacaacg gagaacatcg gtgcaagaac acagatcctg
     2521 gctacaactg cctgccctgc ccaccacgat tcactggctc acagcccttc ggccgaggtg
     2581 tcgaacatgc catggccaac aaacaggtgt gcaaaccgcg aaacccctgc acggacggga
     2641 cgcatgactg caacaagaac gctaagtgca actacctggg tcactacagt gaccccatgt
     2701 accgctgtga gtgcaagccc ggctatgcag gcaatggcat catctgcgga gaggacacag
     2761 acctggacgg ctggcctaat gaaaacctgg tgtgtgtggc caacgcaacc taccactgca
     2821 aaaaggacaa ctgccccaac cttcccaact cggggcagga agactatgac aaggacggga
     2881 ttggcgatgc ctgcgatgat gacgatgaca acgacaagat ccccgatgac agggacaact
     2941 gtccattcca ttacaaccca gcccagtatg actatgacag agatgatgtg ggagaccgct
     3001 gtgacaactg cccctacaac cacaaccctg accaagcaga cacagacaaa aacggggagg
     3061 gcgatgcctg tgctgtggac atcgatggag atggaatcct caatgaacga gacaactgcc
     3121 agtacgttta caacgtggac cagagggaca cggacatgga tggggttgga gatcagtgtg
     3181 acaactgccc cctggaacac aatccagacc agctggactc tgactcagac ctcatagggg
     3241 acacttgtga caacaatcag gacatcgatg aggatggcca tcagaacaat ctggacaact
     3301 gtccctatgt gcctaatgcc aaccaggccg accatgataa agatggcaaa ggagatgcct
     3361 gtgaccatga cgatgacaat gacggcatcc ctgatgacag agacaactgc aggctggtgc
     3421 ccaatcctga ccagaaggac tctgatggtg atggccgagg tgacgcctgc aaagacgact
     3481 ttgaccatga caatgtgcca gatattgatg acatctgtcc tgagaatttt gacatcagtg
     3541 cagcttaccc atacgatgtt ccagattacg cttagctcga gagatctagg ccgctaggcc
     3601 tcacctgcga tctcgatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     3661 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     3721 tcagggggag gtgtgggagg ttttttaaac tagcggccgc aggaacccct agtgatggag
     3781 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc
     3841 cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag
     3901 gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt
     3961 caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     4021 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     4081 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     4141 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg
     4201 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     4261 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcgggct
     4321 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     4381 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca
     4441 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     4501 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     4561 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     4621 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     4681 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     4741 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     4801 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     4861 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     4921 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     4981 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     5041 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     5101 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     5161 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     5221 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     5281 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     5341 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     5401 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     5461 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     5521 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     5581 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     5641 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     5701 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     5761 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     5821 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     5881 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     5941 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     6001 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     6061 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     6121 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     6181 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     6241 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     6301 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     6361 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     6421 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     6481 ggccttttgc tcacatgt
//
