LOCUS       Exported                7162 bp ds-DNA     circular SYN 24-JUN-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pAAV.CMV.SV40.THBS1-HA-KDEL.SV40(polyA)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7162)
  AUTHORS   UNKNOWN
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jun 24, 2020 from SnapGene Viewer 5.1.3.1
            https://www.snapgene.com
COMMENT     ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..7162
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /label=AAV2\ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2
                     vntifkey=34"
                     /note="color: #ffff99"
     enhancer        168..296
                     /label=partial CMV enhancer
                     /label=partial\CMV\enhancer
                     /note="vntifkey=9"
                     /note="color: #c0c0c0; direction: RIGHT"
     promoter        297..500
                     /label=CMV promoter
                     /label=CMV\promoter
                     /note="human cytomegalovirus (CMV) immediate early promoter
                     vntifkey=30"
                     /note="color: #c0c0c0; direction: RIGHT"
     intron          542..634
                     /label=SV40 intron
                     /label=SV40\intron
                     /note="vntifkey=15"
                     /note="color: #------"
     CDS             685..4197
                     /codon_start=1
                     /label=Thbs1
                     /note="vntifkey=4"
                     /note="color: #ff9900"
                     /translation="MELLRGLGVLFLLHMCGSNRIPESGGDNGVFDIFELIGGARRGPG
                     RRLVKGQDLSSPAFRIENANLIPAVPDDKFQDLLDAVWADKGFIFLASLRQMKKTRGTL
                     LAVERKDNTGQIFSVVSNGKAGTLDLSLSLPGKQQVVSVEEALLATGQWKSITLFVQED
                     RAQLYIDCDKMESAELDVPIQSIFTRDLASVARLRVAKGDVNDNFQGVLQNVRFVFGTT
                     PEDILRNKGCSSSATNVLLTLDNNVVNGSSPAIRTNYIGHKTKDLQAICGLSCDELSSM
                     VLELKGLRTIVTTLQDSIRKVTEENRELVSELKRPPLCFHNGVQYKNNEEWTVDSCTEC
                     HCQNSVTICKKVSCPIMPCSNATVPDGECCPRCWPSDSADDGWSPWSEWTSCSATCGNG
                     IQQRGRSCDSLNNRCEGSSVQTRTCHIQECDKRFKQDGGWSHWSPWSSCSVTCGDGVIT
                     RIRLCNSPSPQMNGKPCEGEARETKACKKDACPINGGWGPWSPWDICSVTCGGGVQRRS
                     RLCNNPTPQFGGKDCVGDVTENQVCNKQDCPIDGCLSNPCFAGAKCTSYPDGSWKCGAC
                     PPGYSGNGIQCKDVDECKEVPDACFNHNGEHRCKNTDPGYNCLPCPPRFTGSQPFGRGV
                     EHAMANKQVCKPRNPCTDGTHDCNKNAKCNYLGHYSDPMYRCECKPGYAGNGIICGEDT
                     DLDGWPNENLVCVANATYHCKKDNCPNLPNSGQEDYDKDGIGDACDDDDDNDKIPDDRD
                     NCPFHYNPAQYDYDRDDVGDRCDNCPYNHNPDQADTDKNGEGDACAVDIDGDGILNERD
                     NCQYVYNVDQRDTDMDGVGDQCDNCPLEHNPDQLDSDSDLIGDTCDNNQDIDEDGHQNN
                     LDNCPYVPNANQADHDKDGKGDACDHDDDNDGIPDDRDNCRLVPNPDQKDSDGDGRGDA
                     CKDDFDHDNVPDIDDICPENFDISETDFRQFQMIPLDPKGTSQNDPNWVVRHQGKELVQ
                     TVNCDPGLAVGYDEFNAVDFSGTFFINTERDDDYAGFVFGYQSSSRFYVVMWKQVTQSY
                     WDTNPTRAQGYSGLSVKVVNSTTGPGEHLRNALWHTGNTPGQVRTLWHDPRHIGWKDFT
                     AYRWRLSHRPKTGYIRVVMYEGKKIMADSGPIYDKTYAGGRLGLFVFSQEMVFFSDMKY
                     ECRDS"
     CDS             4198..4224
                     /codon_start=1
                     /label=HA-tag
                     /label=HA
                     /note="vntifkey=4"
                     /note="color: #ff0000"
                     /translation="YPYDVPDYA"
     CDS             4225..4236
                     /codon_start=1
                     /label=KDEL
                     /note="vntifkey=21"
                     /note="color: #00ff00"
                     /translation="KDEL"
     intron          4280..4413
                     /label=SV40 polyA
                     /label=SV40\polyA
                     /note="vntifkey=15"
                     /note="color: #------"
     repeat_region   4425..4565
                     /label=AAV2 ITR
                     /label=AAV2\ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2
                     vntifkey=34"
                     /note="color: #ffff99"
     rep_origin      4640..5095
                     /direction=RIGHT
                     /label=f1 ori
                     /label=f1\ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis
                     vntifkey=33"
                     /note="color: #------"
     promoter        5377..5481
                     /gene="bla"
                     /label=AmpR promoter
                     /label=AmpR\promoter
                     /note="vntifkey=30"
                     /note="color: #800000; direction: RIGHT"
     CDS             5482..6342
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics
                     vntifkey=4"
                     /note="color: #ffcc99"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6513..7101
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication
                     vntifkey=33"
                     /note="color: #------"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgcc gctcgtgacc taggcatatg ccaagtacgc
      181 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      241 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      301 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      361 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      421 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      481 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccgg
      541 actctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg
      601 tttctctctt ttagattcca acctttggaa ctgaattccg cgggcccggg atcctctaga
      661 gtcgacctgc agaagcttat gaccatggag ctcctgcggg gactaggtgt cctgttcctg
      721 ttgcatatgt gtggaagcaa ccgcattcca gagtctgggg gagataacgg tgtgtttgac
      781 atctttgaac tcattggagg tgcacgaagg ggccccggtc gccgactggt gaagggccaa
      841 gatctatcca gccccgcctt ccgcattgag aatgccaacc tgatccccgc tgtgccggat
      901 gacaagttcc aagacctact ggacgctgtg tgggccgaca aaggcttcat cttcctggct
      961 tccttgaggc agatgaagaa gacccggggc acactcctgg ctgtggaacg gaaagacaac
     1021 actggccaga tcttcagtgt ggtctccaac ggcaaagctg gcaccctgga cctgagcctg
     1081 agcctgccag ggaagcaaca agtggtgtca gtggaggaag ctctcctggc cactggccag
     1141 tggaagagca tcacgctgtt tgttcaagag gaccgggctc aactctacat agactgtgat
     1201 aagatggaga gcgcggagct ggatgtaccc atccagagca tcttcaccag ggatctggcc
     1261 agcgttgcca ggctccgagt tgcaaaggga gatgtcaatg acaattttca gggggtgctg
     1321 cagaatgtga ggtttgtctt tggaaccacc ccagaagaca ttctcaggaa caaaggctgc
     1381 tccagctcag ctaccaacgt ccttcttacc cttgacaaca acgtggtgaa cggttccagc
     1441 cctgctatcc gcaccaacta catcggccac aaaacaaagg acctccaagc tatctgtggc
     1501 ctctcctgtg atgaactatc cagcatggtc ctggaactga agggcctgcg caccatcgtg
     1561 accactctgc aggacagcat ccgaaaagtg acggaagaga acagagagct ggtcagtgag
     1621 ctgaagcggc ctcccctctg ctttcacaat ggagtccagt acaagaacaa cgaggagtgg
     1681 actgtagaca gttgcacaga gtgtcactgc cagaactcgg ttaccatctg caaaaaggtg
     1741 tcctgtccca tcatgccctg ctccaacgcc acagttcctg atggtgaatg ctgcccacgg
     1801 tgctggccca gcgactctgc tgacgatggc tggtctccct ggtctgagtg gacctcctgc
     1861 tctgccacat gtggcaatgg aattcagcaa cgtggtcgtt cctgtgacag cctcaacaac
     1921 agatgcgagg gctcttcggt acagacaagg acctgccaca ttcaggagtg tgacaaaaga
     1981 tttaaacagg atggtggctg gagtcactgg tctccatggt cgtcctgttc tgtgacctgt
     2041 ggtgacggtg tgatcacaag gatccggctc tgcaactccc ccagccccca gatgaacggg
     2101 aagccctgtg aaggtgaagc ccgggagacc aaagcctgca agaaagacgc ctgcccaatt
     2161 aatggaggct ggggtccctg gtcaccatgg gacatctgct ctgtcacctg tggaggagga
     2221 gtgcagagac gcagccgact ctgtaacaac cccacacccc agtttggagg caaagactgt
     2281 gttggcgatg tgacagaaaa tcaagtttgc aacaagcagg actgcccaat tgatggatgc
     2341 ctgtccaatc cctgctttgc tggtgccaag tgtactagct accctgatgg tagctggaaa
     2401 tgtggtgcgt gtcctcctgg ctacagtgga aatggcatcc agtgcaaaga cgtcgatgag
     2461 tgcaaagaag tgcctgatgc ttgcttcaat cacaacggag aacatcggtg caagaacaca
     2521 gatcctggct acaactgcct gccctgccca ccacgattca ctggctcaca gcccttcggc
     2581 cgaggtgtcg aacatgccat ggccaacaaa caggtgtgca aaccgcgaaa cccctgcacg
     2641 gacgggacgc atgactgcaa caagaacgct aagtgcaact acctgggtca ctacagtgac
     2701 cccatgtacc gctgtgagtg caagcccggc tatgcaggca atggcatcat ctgcggagag
     2761 gacacagacc tggacggctg gcctaatgaa aacctggtgt gtgtggccaa cgcaacctac
     2821 cactgcaaaa aggacaactg ccccaacctt cccaactcgg ggcaggaaga ctatgacaag
     2881 gacgggattg gcgatgcctg cgatgatgac gatgacaacg acaagatccc cgatgacagg
     2941 gacaactgtc cattccatta caacccagcc cagtatgact atgacagaga tgatgtggga
     3001 gaccgctgtg acaactgccc ctacaaccac aaccctgacc aagcagacac agacaaaaac
     3061 ggggagggcg atgcctgtgc tgtggacatc gatggagatg gaatcctcaa tgaacgagac
     3121 aactgccagt acgtttacaa cgtggaccag agggacacgg acatggatgg ggttggagat
     3181 cagtgtgaca actgccccct ggaacacaat ccagaccagc tggactctga ctcagacctc
     3241 ataggggaca cttgtgacaa caatcaggac atcgatgagg atggccatca gaacaatctg
     3301 gacaactgtc cctatgtgcc taatgccaac caggccgacc atgataaaga tggcaaagga
     3361 gatgcctgtg accatgacga tgacaatgac ggcatccctg atgacagaga caactgcagg
     3421 ctggtgccca atcctgacca gaaggactct gatggtgatg gccgaggtga cgcctgcaaa
     3481 gacgactttg accatgacaa tgtgccagat attgatgaca tctgtcctga gaattttgac
     3541 atcagtgaaa ccgatttccg acaattccag atgattcctc tagatcccaa aggaacctcc
     3601 caaaatgacc ctaactgggt tgtccgccat cagggcaaag aacttgtcca gactgtaaac
     3661 tgtgaccctg gacttgctgt aggttatgat gagtttaatg ctgtggactt cagcggtacc
     3721 ttcttcatca acaccgagag agatgatgac tacgctggct ttgttttcgg ctaccagtcc
     3781 agcagccgct tctacgttgt gatgtggaaa caagtcaccc agtcctactg ggacaccaac
     3841 cccacaaggg ctcagggata ctcaggcctg tctgtaaagg ttgtgaactc caccaccggc
     3901 cctggcgagc acctgcggaa tgcactgtgg cacacaggaa acacccctgg ccaggtgcgc
     3961 accctgtggc atgaccctcg ccacatcggc tggaaagatt tcactgcata cagatggcgt
     4021 ctcagccaca ggccaaagac cggttatatc agagtggtga tgtatgaagg aaagaaaatc
     4081 atggctgact cgggacccat ctatgacaaa acctacgccg gcggtagact aggcctgttc
     4141 gtcttctctc aggaaatggt gttcttctca gacatgaaat acgagtgtcg agattcctac
     4201 ccatacgatg ttccagatta cgctaaagac gaactgtaac tcgagagatc taggccgcta
     4261 ggcctcacct gcgatctcga tgctttattt gtgaaatttg tgatgctatt gctttatttg
     4321 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     4381 aggttcaggg ggaggtgtgg gaggtttttt aaactagcgg ccgcaggaac ccctagtgat
     4441 ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt
     4501 cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct
     4561 gcaggggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     4621 acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     4681 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     4741 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     4801 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt
     4861 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     4921 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     4981 ggctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     5041 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg
     5101 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     5161 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     5221 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     5281 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     5341 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     5401 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     5461 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     5521 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     5581 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     5641 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     5701 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     5761 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     5821 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     5881 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     5941 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     6001 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     6061 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     6121 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     6181 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     6241 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     6301 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     6361 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     6421 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     6481 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     6541 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     6601 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     6661 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     6721 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     6781 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     6841 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     6901 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     6961 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     7021 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     7081 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     7141 tgctggcctt ttgctcacat gt
//
