LOCUS       Exported                8738 bp ds-DNA     circular SYN 24-JUN-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 8738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jun 25, 2020 from SnapGene Viewer 5.1.4.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8738
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     promoter        62..90
                     /label=Amp prom
     CDS             330..989
                     /label=AmpR
     rep_origin      1141..1769
                     /direction=RIGHT
                     /label=ColE1 origin
     promoter        2075..2104
                     /label=lac
     misc_binding    2109..2131
                     /label=LacO
     primer_bind     2137..2157
                     /label=M13-rev
     primer_bind     2175..2194
                     /label=T3
     LTR             2448..2628
                     /label=HIV-1 5 LTR
     misc_feature    2736..2780
                     /label=HIV-1 psi pack
     protein_bind    4746..4770
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_recomb     4747..4753
                     /label=AttR1
     primer_bind     4787..4811
                     /label=RAP2A-F
     misc_feature    4791..4800
                     /label=full ORF
     misc_feature    4791..4799
                     /label=cerulean+linker
     misc_feature    4801..4805
                     /label=HindIII
     CDS             4806..5357
                     /codon_start=1
                     /gene="RAP2A"
                     /gene_synonym="K-REV; KREV; RAP2; RbBP-30"
                     /product="ras-related protein Rap-2a precursor"
                     /label=RAP2A
                     /note="RAP2, member of RAS oncogene family 
                     (K-rev);ras-related protein Rap-2a"
                     /db_xref="CCDS:CCDS9485.1"
                     /db_xref="GeneID:5911"
                     /db_xref="HGNC:HGNC:9861"
                     /db_xref="MIM:179540"
                     /protein_id="NP_066361.1"
                     /translation="MREYKVVVLGSGGVGKNALTVQFVTGTFIEKYDPTIEDFYRKEIE
                     VDSSPSVLEILDTAGTEQFASMRDLYIKNGQGFILVYSLVNQQSFQDIKPMRDQIIRVK
                     RYEKVPVILVGNKVDLESEREVSSSEGRALAEEWGCPFMETSAKSKTMVDELFAEIVRQ
                     MNYAAQPDKDDPCCSACNIQ"
     gene            4806..5357
                     /gene="RAP2A"
                     /gene_synonym="K-REV; KREV; RAP2; RbBP-30"
                     /label=RAP2A
                     /note="RAP2A, member of RAS oncogene family"
                     /db_xref="GeneID:5911"
                     /db_xref="HGNC:HGNC:9861"
                     /db_xref="MIM:179540"
     mat_peptide     4806..5345
                     /gene="RAP2A"
                     /gene_synonym="K-REV; KREV; RAP2; RbBP-30"
                     /product="Ras-related protein Rap-2a"
                     /label=RAP2A (Hsapi)
                     /note="propagated from UniProtKB/Swiss-Prot (P10114.1)"
                     /experiment="experimental evidence, no additional 
                     detailsrecorded"
     exon            4806..5119
                     /gene="RAP2A"
                     /gene_synonym="K-REV; KREV; RAP2; RbBP-30"
                     /inference="alignment:same species:2.1.0"
     CDS             4854..4856
                     /codon_start=1
                     /label=Mutation N17 - Ser for Asp
                     /translation="N"
     misc_feature    4899..4925
                     /gene="RAP2A"
                     /gene_synonym="K-REV; KREV; RAP2; RbBP-30"
                     /note="propagated from UniProtKB/Swiss-Prot 
                     (P10114.1);Region: Effector region. {ECO:0000305}"
                     /experiment="experimental evidence, no additional 
                     detailsrecorded"
     exon            5120..5357
                     /gene="RAP2A"
                     /gene_synonym="K-REV; KREV; RAP2; RbBP-30"
                     /inference="alignment:same species:2.1.0"
     misc_feature    5343..5345
                     /gene="RAP2A"
                     /gene_synonym="K-REV; KREV; RAP2; RbBP-30"
                     /note="Cysteine methyl ester.{ECO:0000305|PubMed:8424780}; 
                     propagated from UniProtKB/Swiss-Prot (P10114.1); 
                     methylation site"
                     /experiment="experimental evidence, no additional 
                     detailsrecorded"
     primer_bind     complement(5349..5369)
                     /label=RAP2A-R
     misc_feature    5358
                     /label=EagI
     protein_bind    complement(5386..5410)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_recomb     complement(5396..5409)
                     /label=AttR2
     CDS             complement(5912..5923)
                     /label=FactorXa site
     promoter        6070..6409
                     /label=mPGK Prom
     CDS             6597..7388
                     /label=Kan/neoR
     misc_feature    7924..8001
                     /label=SV40 ORI
     primer_bind     complement(8091..8110)
                     /label=T7
     primer_bind     complement(8120..8137)
                     /label=M13-fwd
     CDS             8208..8276
                     /label=LacZ alpha
     rep_origin      8281..8736
                     /direction=RIGHT
                     /label=M13 origin
     rep_origin      complement(8286..8726)
                     /direction=LEFT
                     /label=F1 ori
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
     2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
     2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
     3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     3601 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     3661 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     3721 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     3781 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     3841 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     3901 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     3961 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt
     4021 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     4081 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg
     4141 atcacgagac tagcctcgag aagcttgata tcgaattccc acggggttgg ggttgcgcct
     4201 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa
     4261 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg
     4321 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg
     4381 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac
     4441 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc
     4501 tgtggccaat agcggctgct cagcggggcg cgccgagagc agcggccggg aaggggcggt
     4561 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc
     4621 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga
     4681 cctctctccc cagggggatc accggtcgtc gactagtcca gtgtggtgga attctgcaga
     4741 tatcaacaag tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc tctcgagcta
     4801 agcttatgcg cgagtacaaa gtggtggtgc tgggctcggg cggggtaggc aaaaacgccc
     4861 tgaccgtgca gttcgtgacc ggcaccttca tcgagaaata cgaccccacc atcgaggact
     4921 tctaccgcaa ggagatcgag gtggattcgt cgccgtcggt gctggagatc ctggacacgg
     4981 cgggcaccga gcagttcgcg tccatgcggg acctgtacat caagaacggc cagggcttca
     5041 tcctcgtcta cagcctcgtc aaccagcaga gcttccagga catcaagccc atgcgggacc
     5101 agatcatccg cgtgaagcgg tatgagaaag tgccagtcat cttggttggg aacaaagtgg
     5161 acctggaaag tgagagagaa gtatcgtcca gcgaaggcag agcccttgct gaagagtggg
     5221 gctgcccctt tatggaaact tccgctaaga gtaaaacaat ggtggacgaa ctctttgcag
     5281 aaattgtgag gcagatgaac tatgctgctc agcctgacaa agatgaccca tgctgttctg
     5341 catgtaacat acaatagcgg ccgcacagca agggtgggcg cgccgaccca gctttcttgt
     5401 acaaagtggt tgatatccag cacagtggcg gccgctcgac aatcaacctc tggattacaa
     5461 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     5521 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     5581 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     5641 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     5701 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     5761 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     5821 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat
     5881 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     5941 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     6001 tcggatctcc ctttgggccg cctccccgcc tggaattctg cagatatccg gactagtgat
     6061 ctaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc
     6121 agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc
     6181 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc
     6241 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat
     6301 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg
     6361 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag
     6421 gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc
     6481 ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct
     6541 gttctcctct tcctcatctc cgggcctttc gacctgcagc caatatggga tcggccattg
     6601 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     6661 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     6721 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
     6781 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     6841 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     6901 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     6961 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     7021 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     7081 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg
     7141 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     7201 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     7261 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     7321 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     7381 tcttctgagg ggatccgtcg agggcccggt acctttaaga ccaatgactt acaaggcagc
     7441 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctag ctcactccca
     7501 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     7561 ctgggagctc tctggctgcc tagggaaccc actgcttaag cctcaataaa gcttgccttg
     7621 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     7681 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     7741 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     7801 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     7861 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     7921 gctatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     7981 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     8041 ggaggctttt ttggaggcct aggcttttgc gtcgagacgt acccaattcg ccctatagtg
     8101 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     8161 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     8221 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcg
     8281 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     8341 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     8401 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     8461 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     8521 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     8581 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     8641 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     8701 acgcgaattt taacaaaata ttaacgttta caatttcc
//
