LOCUS       421_24__pTYF_sup       12522 bp ds-DNA     circular     07-AUG-2018
SOURCE      
  ORGANISM  
COMMENT     revamped version with oligos ATSceTyf 5 & 6
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     2115..2132
                     /locus_tag="TPH2 NotI Venus Fw"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          201..2110
                     /organism="Macaca mulatta"
                     /mol_type="genomic DNA"
                     /isolate="17573"
                     /db_xref="taxon:9544"
                     /chromosome="16"
                     /sex="female"
                     /country="USA: Southwest National Primate Research Center
                     at the Southwest Foundation for Biomedical Research, San
                     Antonio, TX"
                     /note="derived from Indian origin rhesus"
                     /locus_tag="derived from Indian origin rhesus"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          2111..2112
                     /organism="Macaca mulatta"
                     /mol_type="genomic DNA"
                     /isolate="17573"
                     /db_xref="taxon:9544"
                     /chromosome="16"
                     /sex="female"
                     /country="USA: Southwest National Primate Research Center
                     at the Southwest Foundation for Biomedical Research, San
                     Antonio, TX"
                     /note="derived from Indian origin rhesus"
                     /locus_tag="derived from Indian origin rhesus(1)"
                     /ApEinfo_label="derived from Indian origin rhesus"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4255..5115
                     /locus_tag="sequence checked"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3514..4477
                     /locus_tag="sequence checked(1)"
                     /ApEinfo_label="sequence checked"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            1976..2110
                     /gene="SLC6A4"
                     /note="solute carrier family 6 (neurotransmitter
                     transporter, serotonin), member 4; Derived by automated
                     computational analysis using gene prediction method:
                     BestRefSeq,Gnomon."
                     /db_xref="GeneID:574140"
                     /locus_tag="SLC6A4"
                     /ApEinfo_fwdcolor="#ff797d"
                     /ApEinfo_revcolor="#ff102f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_hidden
     misc_feature    2900..3887
                     /locus_tag="sequence checked(2)"
                     /ApEinfo_label="sequence checked"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     mRNA            1976..2110
                     /gene="SLC6A4"
                     /product="solute carrier family 6 (neurotransmitter
                     transporter), member 4, transcript variant X1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Gnomon. Supporting evidence
                     includes similarity to: 6 mRNAs, 28 ESTs, 11 Proteins, and
                     100% coverage of the annotated genomic feature by RNAseq
                     alignments, including 5 samples with support for all
                     annotated introns"
                     /transcript_id="XM_015118835.1"
                     /db_xref="GeneID:574140"
                     /locus_tag="solute carrier family 6 (neurotransmitter
                     transporter), member 4, transcript variant X1"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_hidden
     gene            2111..2112
                     /gene="SLC6A4"
                     /note="solute carrier family 6 (neurotransmitter
                     transporter, serotonin), member 4; Derived by automated
                     computational analysis using gene prediction method:
                     BestRefSeq,Gnomon."
                     /db_xref="GeneID:574140"
                     /locus_tag="SLC6A4(1)"
                     /ApEinfo_label="SLC6A4"
                     /ApEinfo_fwdcolor="#ff797d"
                     /ApEinfo_revcolor="#ff102f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    10540..10677
                     /vntifkey="21"
                     /locus_tag="HIV-1\Genomic\RNA\Packing\Signal\(Psi)"
                     /ApEinfo_fwdcolor="#cfcfcf"
                     /ApEinfo_revcolor="#cfcfcf"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     mRNA            2111..2112
                     /gene="SLC6A4"
                     /product="solute carrier family 6 (neurotransmitter
                     transporter), member 4, transcript variant X1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Gnomon. Supporting evidence
                     includes similarity to: 6 mRNAs, 28 ESTs, 11 Proteins, and
                     100% coverage of the annotated genomic feature by RNAseq
                     alignments, including 5 samples with support for all
                     annotated introns"
                     /transcript_id="XM_015118835.1"
                     /db_xref="GeneID:574140"
                     /locus_tag="solute carrier family 6 (neurotransmitter
                     transporter), member 4, transcript variant X1(1)"
                     /ApEinfo_label="solute carrier family 6 (neurotransmitter
                     transporter), member 4, transcript variant X1"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    186..187
                     /vntifkey="31"
                     /note="GAL-4 binding sites"
                     /locus_tag="GAL4-BS"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          2877..3356
                     /organism="pIRES2-EGFP"
                     /mol_type="other DNA"
                     /locus_tag="source:pIRES2-EGFP"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    36..185
                     /vntifkey="31"
                     /note="GAL-4 binding sites"
                     /locus_tag="GAL4-BS(1)"
                     /ApEinfo_label="GAL4-BS"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     186..194
                     /locus_tag="156 invPCR Rv"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_signal     4558..5133
                     /locus_tag="WPRE"
                     /ApEinfo_fwdcolor="#00ff40"
                     /ApEinfo_revcolor="#00ff40"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    201..222
                     /locus_tag="primer 30 F"
                     /ApEinfo_fwdcolor="#ff0080"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    10309..10488
                     /vntifkey="21"
                     /locus_tag="5'\LRT"
                     /ApEinfo_fwdcolor="#cfcfcf"
                     /ApEinfo_revcolor="#cfcfcf"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2877..3356
                     /locus_tag="IRES"
                     /ApEinfo_fwdcolor="#c0c0c0"
                     /ApEinfo_revcolor="#c0c0c0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    11881..12085
                     /vntifkey="21"
                     /locus_tag="RRE"
                     /ApEinfo_fwdcolor="#cfcfcf"
                     /ApEinfo_revcolor="#cfcfcf"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             3453..3464
                     /gene="EGFP"
                     /note="ORF frame 3"
                     /translation="MATTMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATY
                     GKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERT
                     IFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMAD
                     KQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNE
                     KRDHMVLLEFVTAAGITLGMDELYK*"
                     /locus_tag="EGFP"
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="#0000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1561..1584
                     /locus_tag="primer 31F,69R"
                     /ApEinfo_fwdcolor="#ff0080"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             7677..8537
                     /vntifkey="4"
                     /locus_tag="Ampicillin\Resistance\Gene"
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="#0000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    872..888
                     /locus_tag="primer 67,68"
                     /ApEinfo_fwdcolor="#ff0080"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    12365..12483
                     /vntifkey="21"
                     /locus_tag="cPPT-DNA\FLAP"
                     /ApEinfo_fwdcolor="#cfcfcf"
                     /ApEinfo_revcolor="#cfcfcf"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5154..5171
                     /vntifkey="21"
                     /locus_tag="I-SceI"
                     /ApEinfo_fwdcolor="#00ff40"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5345..5602
                     /vntifkey="21"
                     /locus_tag="bGHpA"
                     /ApEinfo_fwdcolor="#cfcfcf"
                     /ApEinfo_revcolor="#cfcfcf"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5..22
                     /vntifkey="21"
                     /locus_tag="I-SceI(1)"
                     /ApEinfo_label="I-SceI"
                     /ApEinfo_fwdcolor="#00ff40"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    12409..12428
                     /locus_tag="pTYF out Fw1"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5..5
                     /locus_tag="T?"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5134..5148
                     /locus_tag="WPRE(1)"
                     /ApEinfo_label="WPRE"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     exon            3465..4526
                     /vntifkey="61"
                     /locus_tag="Gal4p65"
                     /ApEinfo_fwdcolor="#ff80c0"
                     /ApEinfo_revcolor="#ff80c0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          3358..3464
                     /organism="pIRES2-EGFP"
                     /mol_type="other DNA"
                     /locus_tag="source:pIRES2-EGFP(1)"
                     /ApEinfo_label="source:pIRES2-EGFP"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3358..3461
                     /locus_tag="IRES(1)"
                     /ApEinfo_label="IRES"
                     /ApEinfo_fwdcolor="#c0c0c0"
                     /ApEinfo_revcolor="#c0c0c0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3357..3357
                     /locus_tag="New Feature"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5268..5346
                     /locus_tag="LTR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     170..185
                     /locus_tag="156 invPCR Rv(1)"
                     /ApEinfo_label="156 invPCR Rv"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     2865..2877
                     /locus_tag="156 invPCR Fw +AscI"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             2130..2849
                     /locus_tag="Venus"
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="#0000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     2830..2864
                     /locus_tag="Venus AscI Rv"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2115..2827
                     /locus_tag="sequence verified"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    195..195
                     /locus_tag="Mlu I"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2115..2120
                     /locus_tag="Not I"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2083..2110
                     /locus_tag="pr32R"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    758..961
                     /locus_tag="sequence mada"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 ggcctaggga taacagggta atagatcttg ctagctctta tcatgtctgg atccaagctt
       61 gcatgcctgc aggtcggagt actgtcctcc gagcggagta ctgtcctccg agcggagtac
      121 tgtcctccga gcggagtact gtcctccgag cggagtactg tcctccgagc ggagactcta
      181 gagggtcccg ggatACGCGT ctctgaatgc cagcacctaa ccccctaatg tccctgctgc
      241 agccctccca gcatctccct gtacccctcc taggatctcc cctgcacccc ccattatcct
      301 ccctacaccc cccagcatcc cccctgcagc ctcccagcat ctcccctgca ccccccagca
      361 tcccccctgc agccctttca gcatccccct gcacccctcc caggatctcc cttgcatccc
      421 cattatctcc cctgcactcc tcgcagtatc cccccgcacc tccattatcc cccctgcacc
      481 ccttgcggca tcccccctgc accccccagt attccccctg cagcaccccc cagcatctcc
      541 cccgcaccgc ccagcatccc ccctgcagcc cttccagcaa ccccctgcat ccctcccagg
      601 atctcccctg caaccccatt atcccccctg cacccctcgc ggcatccccc tacacccccc
      661 aacataccct ctgcaccccc cggaatcccc cctgcacccc tccagcattc cccttgcacc
      721 ctcccagtat ccccctgcat cccgggctcc aagcctcccg cccaccttgc ggtccccgcc
      781 ctggcgtcta ggtggcacca gaatcccgcg cggactcctc ccgctgggtg ccgccctcgc
      841 ttgcccatgg ttgtccagct cagtccccct ctagacgctc agcccaaccg gccgcacagt
      901 tttcaggggt cagttcctcc aagtacaagg ggctgtggct tctctggagc tgcaaacttg
      961 ttactgctat ttcctttcgg tcttctactt cctatcgttc ctggcctctt cttggggaga
     1021 ggtagagcct tctcctctct gcctcaggga caacccaaag caagtactgc atgtgccctt
     1081 tttaaagttt taaataattt tagcaaaaag ggtattaaca ttaaatccat ttttaaactt
     1141 tttgaaaaaa attatcaaaa taacatgcac atggttcaaa acaataggct cctgctgggc
     1201 gctttcagat aattcaaatt gtcacccagg tttgagtgca gtggctccat cacggctcac
     1261 tgcagcctcg acctcctggg ctcagctgat cctccacctc agcctcgtga gtagctggga
     1321 ccacaggtgc gggcaaccac gcccggttaa ttaaaaaaaa attttttttt tttctagaga
     1381 tagggtcttg ctgtgttgcc caggctagtc tcgaattatt gggctcaagc gatcctcccc
     1441 cgcctcagcc tcccaaagca ctgtgctccg ttttgacgca gctttgaaca gtagctggtt
     1501 aacaaaatga gaaccagttc ttcattcctt cattgtggaa gtctttattg tgagactctg
     1561 gggacagaga ggaattagac aaggctaagc tgaactcaca tcccagcagt cagataaacg
     1621 catgggtacc aagtactgtt aacgtcccag gaagaaaaag caggtttcgg gatgggggcg
     1681 atggggaggt gttggaggtc aagaggaagc ggcaccagca gacccctatg tgccgttctg
     1741 ggggaggggg gcggcagggg aggcgcatac cccgtgcccc gttgtgcagc ctcccccctc
     1801 ccgcaaagtt aaagagcagg aaagtcagga ttcctcgctc ggccctgccc taccctgcct
     1861 gctgctccgc gctccgctcc tccctgcgag cgtgtgtgtg tcgggggtcc ctcccctcct
     1921 ggcgctgggg tcgggcgcgc accccgccca gtagcgcggc ccctcccggg cgagcgcaac
     1981 cccatccagc gggagcgccg agccgcggcc gcggggaagc attaagttta ttcgcctcaa
     2041 agtgacgcaa aaattcttca agagctcttt ggcggcggct atctagagat cagaccatgt
     2101 gaggaccggt GAGCGGCCGC GGATCCACCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG
     2161 GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT
     2221 CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGCTG ATCTGCACCA
     2281 CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGGGCTAC GGCCTGCAGT
     2341 GCTTCGCCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG
     2401 AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG
     2461 CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT
     2521 TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACAAC AGCCACAACG
     2581 TCTATATCAC CGCCGACAAG CAGAAGAACG GCATCAAGGC CAACTTCAAG ATCCGCCACA
     2641 ACATCGAGGA CGGCGGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC CCCATCGGCG
     2701 ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCTA CCAGTCCGCC CTGAGCAAAG
     2761 ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA
     2821 CTCTCGGCAT GGACGAGCTG TACAAGTAAg gcgcgccCTA GTcggatatc cgtcgaGCCC
     2881 CTCTCCCTCC CCCCCCCCTA ACGTTACTGG CCGAAGCCGC TTGGAATAAG GCCGGTGTGC
     2941 GTTTGTCTAT ATGTTATTTT CCACCATATT GCCGTCTTTT GGCAATGTGA GGGCCCGGAA
     3001 ACCTGGCCCT GTCTTCTTGA CGAGCATTCC TAGGGGTCTT TCCCCTCTCG CCAAAGGAAT
     3061 GCAAGGTCTG TTGAATGTCG TGAAGGAAGC AGTTCCTCTG GAAGCTTCTT GAAGACAAAC
     3121 AACGTCTGTA GCGACCCTTT GCAGGCAGCG GAACCCCCCA CCTGGCGACA GGTGCCTCTG
     3181 CGGCCAAAAG CCACGTGTAT AAGATACACC TGCAAAGGCG GCACAACCCC AGTGCCACGT
     3241 TGTGAGTTGG ATAGTTGTGG AAAGAGTCAA ATGGCTCTCC TCAAGCGTAT TCAACAAGGG
     3301 GCTGAAGGAT GCCCAGAAGG TACCCCATTG TATGGGATCT GATCTGGGGC CTCGGTACAC
     3361 ATGCTTTACA TGTGTTTAGT CGAGGTTAAA AAAACGTCTA GGCCCCCCGA ACCACGGGGA
     3421 CGTGGTTTTC CTTTGAAAAA CACGATGATA ATATGGCCAC AACCatgaag ctactgtctt
     3481 ctatcgaaca agcatgcgat atttgccgac ttaaaaagct caagtgctcc aaagaaaaac
     3541 cgaagtgcgc caagtgtctg aagaacaact gggagtgtcg ctactctccc aaaaccaaaa
     3601 ggtctccgct gactagggca catctgacag aagtggaatc aaggctagaa agactggaac
     3661 agctatttct actgattttt cctcgagaag accttgacat gattttgaaa atggattctt
     3721 tacaggatat aaaagcattg ttaacaggat tatttgtaca agataatgtg aataaagatg
     3781 ccgtcacaga tagattggct tcagtggaga ctgatatgcc tctaacattg agacagcata
     3841 gaataagtgc gacatcatca tcggaagaga gtagtaacaa aggtcaaaga cagttgactg
     3901 tatcgccgga attgggatcc atggtgtttc cttctgggca gatctcaaac caggccctgg
     3961 ccttagcacc gtcctctgcc ccagtccttg cccagaccat ggtcccttcc tcagccatgg
     4021 tacctctggc tcagccccca gctcctgccc cagttctaac cccgggtcct ccccagtccc
     4081 tgtctgcacc tgttccaaag agcacccagg ctggggaagg cacgctgtcg gaagccctgc
     4141 tgcacctgca gtttgatgct gatgaagact tgggggcctt gcttggcaac agcacagacc
     4201 caggagtgtt cacagacctg gcatctgtgg acaactcaga gtttcagcag ctcctgaacc
     4261 agggtgtgtc catgtctcac tccacagctg agcccatgct gatggagtac cctgaagcta
     4321 taactcgcct ggtgacaggg tcccagaggc cccctgaccc agctcccaca cccctgggga
     4381 cctcggggct tcccaatggt ctctccggag atgaagactt ctcctccatt gcggacatgg
     4441 acttctctgc tctgctgagt cagatcagct ccagcggcca atctagactg cagctcgacc
     4501 tcgagggggg gcccggtacc aggtaaGTTA ACATCGAGGG ATCAAGCTTA TCGATAATCA
     4561 ACCTCTGGAT TACAAAATTT GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT
     4621 TACGCTATGT GGATACGCTG CTTTAATGCC TTTGTATCAT GCTATTGCTT CCCGTATGGC
     4681 TTTCATTTTC TCCTCCTTGT ATAAATCCTG GTTGCTGTCT CTTTATGAGG AGTTGTGGCC
     4741 CGTTGTCAGG CAACGTGGCG TGGTGTGCAC TGTGTTTGCT GACGCAACCC CCACTGGTTG
     4801 GGGCATTGCC ACCACCTGTC AGCTCCTTTC CGGGACTTTC GCTTTCCCCC TCCCTATTGC
     4861 CACGGCGGAA CTCATCGCCG CCTGCCTTGC CCGCTGCTGG ACAGGGGCTC GGCTGTTGGG
     4921 CACTGACAAT TCCGTGGTGT TGTCGGGGAA GCTGACGTCC TTTCCATGGC TGCTCGCCTG
     4981 TGTTGCCACC TGGATTCTGC GCGGGACGTC CTTCTGCTAC GTCCCTTCGG CCCTCAATCC
     5041 AGCGGACCTT CCTTCCCGCG GCCTGCTGCC GGCTCTGCGG CCTCTTCCGC GTCTTCGCCT
     5101 TCGCCCTCAG ACGAGTCGGA TCTCCCTTTG GGCCGCCTCC CCGCATCGtc gactagggat
     5161 aacagggtaa tggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     5221 tttttaaaag aaaagggggg actggaaggg ctaattcact cccagggtct ctctggttag
     5281 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     5341 aaagctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     5401 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     5461 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     5521 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     5581 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
     5641 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     5701 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tgtgcgggag
     5761 aacggagttc tattatgact caaatcagtc tccccaagca ttcggggatc agagttttta
     5821 aggataactt agtgtgtagg gggccagtga gttggagatg aaagcgtagg gagtcgaagg
     5881 tgtccttttg cgccgagtca gttcctgggt gggggccaca agatcggatg agccagttta
     5941 tcaatccggg ggtgccagct gatccatgga gtgcagggtc tgcaaaatat ctcaagcact
     6001 gattgatctt aggttttaca atagtgatgt taccccagga acaatttggg gaaggtcaga
     6061 atcttgtagc ctgtagctgc atgactccta aaccataatt tcttttttgt tttttttttt
     6121 ttatttttga gacagggtct cactctgtca cctaggctgg agtgcagtgg tgcaatcaca
     6181 gctcactgca gcctcaacgt cgtaagctca agcgatcctc ccacctcagc ctgcctggta
     6241 gctgagacta caagcgacgc cccagttaat ttttgtattt ttggtagagg cagcgttttg
     6301 ccgtgtggcc ctggctggtc tcgaactcct gggctcaagt gatccagcct cagcctccca
     6361 aagtgctggg acaaccgggg ccagtcactg cacctggccc taaaccataa tttctaatct
     6421 tttggctaat ttgttagtcc tacaaaggca gtctagtccc caggcaaaaa gggggtttgt
     6481 ttcgggaaag ggctgttact gtctttgttt caaactataa actaagttcc tcctaaactt
     6541 agttcggcct acacccagga atgaacaagg agagcttgga ggttagaagc acgatggaat
     6601 tggttaggtc agatctcttt cactgtctga gttataattt tgcaatggtg gttcaaagac
     6661 tgcccgcttc tgacaccagt cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     6721 tttgcgtatt ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     6781 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     6841 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     6901 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     6961 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     7021 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     7081 tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg
     7141 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     7201 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     7261 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     7321 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     7381 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     7441 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     7501 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     7561 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     7621 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     7681 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     7741 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     7801 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     7861 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     7921 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     7981 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     8041 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     8101 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     8161 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     8221 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     8281 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     8341 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     8401 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     8461 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     8521 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     8581 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     8641 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     8701 acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaactgcc tcgcgcgttt
     8761 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
     8821 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
     8881 tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtactggc ttaactatgc
     8941 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     9001 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
     9061 agggcgatcg gtgcgggcct cttcgctatt acgccagcgc ggggaggcag agattgcagt
     9121 aagctgagat cgcagcactg cactccagcc tgggcgacag agtaagactc tgtctcaaaa
     9181 ataaaataaa taaatcaatc agatattcca atcttttcct ttatttattt atttattttc
     9241 tattttggaa acacagtcct tccttattcc agaattacac atatattcta tttttcttta
     9301 tatgctccag ttttttttag accttcacct gaaatgtgtg tatacaaaat ctaggccagt
     9361 ccagcagagc ctaaaggtaa aaaataaaat aataaaaaat aaataaaatc tagctcactc
     9421 cttcacatca aaatggagat acagctgtta gcattaaata ccaaataacc catcttgtcc
     9481 tcaataattt taagcgcctc tctccaccac atctaactcc tgtcaaaggc atgtgcccct
     9541 tccgggcgct ctgctgtgct gccaaccaac tggcatgtgg actctgcagg gtccctaact
     9601 gccaagcccc acagtgtgcc ctgaggctgc cccttccttc tagcggctgc ccccactcgg
     9661 ctttgctttc cctagtttca gttacttgcg ttcagccaag gtctgaaact aggtgcgcac
     9721 agagcggtaa gactgcgaga gaaagagacc agctttacag ggggtttatc acagtgcacc
     9781 ctgacagtcg tcagcctcac agggggttta tcacattgca ccctgacagt cgtcagcctc
     9841 acagggggtt tatcacagtg cacccttaca atcattccat ttgattcaca atttttttag
     9901 tctctactgt gcctaacttg taagttaaat ttgatcagag gtgtgttccc agaggggaaa
     9961 acagtatata cagggttcag tactatcgca tttcagggtg gcccgagagc tgcatccgga
    10021 gtatctagat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
    10081 ccaacgaccc ccgcccattg acgtcaataa tgactgatgt tcccatagta acgccaatag
    10141 ggactttcca ttgacgctaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
    10201 aaatgtcgta ataaccccgc cccgttgacg caaatgggcg gtaggcgtgt actctagaag
    10261 gtctatataa gcagagctcg tttagtgaac cgtttatact acttatctgg tctctctggt
    10321 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
    10381 aataaagctt gccttgagtg ctcaaagtag tgtgtgcccg tctgttgtgt gactctggta
    10441 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
    10501 cagggacttg aaagcgaaag taaagccaga ggagatctct cgacgcagga ctcggcttgc
    10561 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
    10621 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc ggtattaagc gggggagaat
    10681 tagataaatg ggaaaaaatt cggttaaggc cagggggaaa gaaacaatat aaactaaaac
    10741 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc cttttagaga
    10801 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
    10861 aagaacttag atcattatat aatacaatag cagtcctcta ttgtgtgcat caaaggatag
    10921 atgtaaaaga caccaaggaa gccttagata agatagagga agagcaaaac aaaagtaaga
    10981 aaaaggcaca gcaagcagca gctgacacag gaaacaacag ccaggtcagc caaaattacc
    11041 ctatagtgca gaacctccag gggcaaatgg tacatcaggc catatcacct agaactttaa
    11101 atgcatgggt aaaagtagta gaagagaagg ctttcagccc agaagtaata cccatgtttt
    11161 cagcattatc agaaggagcc accccacaag atttaaatac catgctaaac acagtggggg
    11221 gacatcaagc agccatgcaa atgttaaaag agaccatcaa tgaggaagct gcagaatggg
    11281 atagattgca tccagtgcat gcagggccta ttgcaccagg ccagatgaga gaaccaaggg
    11341 gaagtgacat agcaggaact actagcaaat taagagaaca atttggaaat aataaaacaa
    11401 taatctttaa gcaatcctca ggaggggacc cagaaattgt aacgcacagt tttaattgtg
    11461 gaggggaatt tttctactgt aattcaacac aactgtttaa tagtacttgg tttaatagta
    11521 cttggagtac tgaagggtca aataacactg aaggaagtga cacaatcaca ctcccatgca
    11581 gaataaaaca atttataaac atgtggcagg aagtaggaaa agcaatgtat gcccctccca
    11641 tcagtggaca aattagatgt tcatcaaata ttactgggct gctattaaca agagatggtg
    11701 gtaataacaa caatgggtcc gagatcttca gacctggagg aggcgatatg agggacaatt
    11761 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
    11821 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
    11881 tccttgggtt cttgggagca gcaggaagca ctatgggcgc acggtcaatg acgctgacgg
    11941 tacaggccag acaattattg tctgatatag tgcagcagca gaacaatttg ctgagggcta
    12001 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaaacag ctccaggcaa
    12061 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
    12121 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
    12181 tggaacagat ttggaataac atgacctgga tggagtggga cagagaaatt aacaattaca
    12241 caAgcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
    12301 aattattgga attagataaa tgggcttgcg gccggaattc acaaatggca gtattcatcc
    12361 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
    12421 taatagcaac agacatacaa actaaagaat tacaaaaaca aattactaaa aattcnaaaa
    12481 ttttcgggtt tattacaggg acagcagaga tccactttgg gc 
//
