LOCUS       427_30__pTYF_sup       12283 bp ds-DNA     circular     07-AUG-2018
SOURCE      
  ORGANISM  
COMMENT     revamped version with oligos ATSceTyf 5 & 6
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    923..1098
                     /locus_tag="not read sequence"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="#00ff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     1876..1893
                     /locus_tag="TPH2 NotI Venus Fw"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          201..1873
                     /organism="Macaca mulatta"
                     /mol_type="genomic DNA"
                     /isolate="17573"
                     /db_xref="taxon:9544"
                     /chromosome="3"
                     /sex="female"
                     /country="USA: Southwest National Primate Research Center
                     at the Southwest Foundation for Biomedical Research, San
                     Antonio, TX"
                     /note="derived from Indian origin rhesus"
                     /locus_tag="derived from Indian origin rhesus"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4016..4876
                     /locus_tag="sequence checked"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            1691..1873
                     /gene="TAC1"
                     /note="tachykinin, precursor 1; Derived by automated
                     computational analysis using gene prediction method:
                     BestRefSeq,Gnomon."
                     /db_xref="GeneID:697350"
                     /locus_tag="TAC1"
                     /ApEinfo_fwdcolor="#ff797d"
                     /ApEinfo_revcolor="#ff102f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3275..4238
                     /locus_tag="sequence checked(1)"
                     /ApEinfo_label="sequence checked"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     mRNA            1691..1873
                     /gene="TAC1"
                     /product="tachykinin precursor 1, transcript variant X1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Gnomon. Supporting evidence
                     includes similarity to: 8 mRNAs, 45 ESTs, 4 Proteins, and
                     99% coverage of the annotated genomic feature by RNAseq
                     alignments, including 33 samples with support for all
                     annotated introns"
                     /transcript_id="XM_015134258.1"
                     /db_xref="GeneID:697350"
                     /locus_tag="tachykinin precursor 1, transcript variant X1"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2661..3648
                     /locus_tag="sequence checked(2)"
                     /ApEinfo_label="sequence checked"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     mRNA            1732..1873
                     /gene="TAC1"
                     /product="tachykinin precursor 1, transcript variant X3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Gnomon. Supporting evidence
                     includes similarity to: 2 mRNAs, 15 ESTs, 2 Proteins, and
                     100% coverage of the annotated genomic feature by RNAseq
                     alignments, including 31 samples with support for all
                     annotated introns"
                     /transcript_id="XM_015134260.1"
                     /db_xref="GeneID:697350"
                     /locus_tag="tachykinin precursor 1, transcript variant X3"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    10301..10438
                     /vntifkey="21"
                     /locus_tag="HIV-1\Genomic\RNA\Packing\Signal\(Psi)"
                     /ApEinfo_fwdcolor="#cfcfcf"
                     /ApEinfo_revcolor="#cfcfcf"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     mRNA            1734..1873
                     /gene="TAC1"
                     /product="tachykinin precursor 1, transcript variant X2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Gnomon. Supporting evidence
                     includes similarity to: 2 mRNAs, 21 ESTs, 3 Proteins, and
                     100% coverage of the annotated genomic feature by RNAseq
                     alignments, including 33 samples with support for all
                     annotated introns"
                     /transcript_id="XM_015134259.1"
                     /db_xref="GeneID:697350"
                     /locus_tag="tachykinin precursor 1, transcript variant X2"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    186..187
                     /vntifkey="31"
                     /note="GAL-4 binding sites"
                     /locus_tag="GAL4-BS"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     mRNA            1752..1873
                     /gene="TAC1"
                     /product="tachykinin precursor 1"
                     /inference="similar to RNA sequence, mRNA (same
                     species):RefSeq:NM_001257461.2"
                     /exception="annotated by transcript or proteomic data"
                     /note="The RefSeq transcript has 5 substitutions compared
                     to this genomic sequence; Derived by automated
                     computational analysis using gene prediction method:
                     BestRefSeq."
                     /transcript_id="NM_001257461.2"
                     /db_xref="GeneID:697350"
                     /locus_tag="tachykinin precursor 1"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          2638..3117
                     /organism="pIRES2-EGFP"
                     /mol_type="other DNA"
                     /locus_tag="source:pIRES2-EGFP"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    36..185
                     /vntifkey="31"
                     /note="GAL-4 binding sites"
                     /locus_tag="GAL4-BS(1)"
                     /ApEinfo_label="GAL4-BS"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     186..194
                     /locus_tag="156 invPCR Rv"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_signal     4319..4894
                     /locus_tag="WPRE"
                     /ApEinfo_fwdcolor="#00ff40"
                     /ApEinfo_revcolor="#00ff40"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    10070..10249
                     /vntifkey="21"
                     /locus_tag="5'\LRT"
                     /ApEinfo_fwdcolor="#cfcfcf"
                     /ApEinfo_revcolor="#cfcfcf"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    201..220
                     /locus_tag="primer 39 F1"
                     /ApEinfo_fwdcolor="#ff0080"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2638..3117
                     /locus_tag="IRES"
                     /ApEinfo_fwdcolor="#c0c0c0"
                     /ApEinfo_revcolor="#c0c0c0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1854..1873
                     /locus_tag="primer 41 R"
                     /ApEinfo_fwdcolor="#408080"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    11642..11846
                     /vntifkey="21"
                     /locus_tag="RRE"
                     /ApEinfo_fwdcolor="#cfcfcf"
                     /ApEinfo_revcolor="#cfcfcf"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1054..1078
                     /locus_tag="primer 40 F2"
                     /ApEinfo_fwdcolor="#ff0080"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             3214..3225
                     /gene="EGFP"
                     /note="ORF frame 3"
                     /translation="MATTMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATY
                     GKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERT
                     IFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMAD
                     KQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNE
                     KRDHMVLLEFVTAAGITLGMDELYK*"
                     /locus_tag="EGFP"
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="#0000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             7438..8298
                     /vntifkey="4"
                     /locus_tag="Ampicillin\Resistance\Gene"
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="#0000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    12126..12244
                     /vntifkey="21"
                     /locus_tag="cPPT-DNA\FLAP"
                     /ApEinfo_fwdcolor="#cfcfcf"
                     /ApEinfo_revcolor="#cfcfcf"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4915..4932
                     /vntifkey="21"
                     /locus_tag="I-SceI"
                     /ApEinfo_fwdcolor="#00ff40"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5106..5363
                     /vntifkey="21"
                     /locus_tag="bGHpA"
                     /ApEinfo_fwdcolor="#cfcfcf"
                     /ApEinfo_revcolor="#cfcfcf"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5..22
                     /vntifkey="21"
                     /locus_tag="I-SceI(1)"
                     /ApEinfo_label="I-SceI"
                     /ApEinfo_fwdcolor="#00ff40"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    12170..12189
                     /locus_tag="pTYF out Fw1"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5..5
                     /locus_tag="T?"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4895..4909
                     /locus_tag="WPRE(1)"
                     /ApEinfo_label="WPRE"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     exon            3226..4287
                     /vntifkey="61"
                     /locus_tag="Gal4p65"
                     /ApEinfo_fwdcolor="#ff80c0"
                     /ApEinfo_revcolor="#ff80c0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          3119..3225
                     /organism="pIRES2-EGFP"
                     /mol_type="other DNA"
                     /locus_tag="source:pIRES2-EGFP(1)"
                     /ApEinfo_label="source:pIRES2-EGFP"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3119..3222
                     /locus_tag="IRES(1)"
                     /ApEinfo_label="IRES"
                     /ApEinfo_fwdcolor="#c0c0c0"
                     /ApEinfo_revcolor="#c0c0c0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3118..3118
                     /locus_tag="New Feature"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5029..5107
                     /locus_tag="LTR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     170..185
                     /locus_tag="156 invPCR Rv(1)"
                     /ApEinfo_label="156 invPCR Rv"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     2626..2638
                     /locus_tag="156 invPCR Fw +AscI"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             1891..2610
                     /locus_tag="Venus"
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="#0000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     2591..2625
                     /locus_tag="Venus AscI Rv"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1876..2588
                     /locus_tag="sequence verified"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    195..195
                     /locus_tag="Mlu I"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1876..1881
                     /locus_tag="Not I"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 ggcctaggga taacagggta atagatcttg ctagctctta tcatgtctgg atccaagctt
       61 gcatgcctgc aggtcggagt actgtcctcc gagcggagta ctgtcctccg agcggagtac
      121 tgtcctccga gcggagtact gtcctccgag cggagtactg tcctccgagc ggagactcta
      181 gagggtcccg ggatACGCGT tttacgtcac ccagaccatg gtactttctt acagcagcct
      241 gaactaacaa aaccaattat aatatgtgga cttcattata attctgattt caactagtaa
      301 actgcgtggg gaggaattag gaggcaatga gaaatatttg aatgctaact ggatatttaa
      361 ggtaaggaat tattttatgt tgttttaggt gggaaaatgg tatggttatg tttttatttt
      421 tttaaatgct taccttttat acagtgatgc tgaaatattt acagttgaaa tgactaaatg
      481 tctgggattt tttttcaaaa taatttgagt gcagaggaaa ctggaagggt tgtagatgaa
      541 acctggaagt ggatgtacct ctctactttt ggagatgctg ataattttcc atataaaatt
      601 tctgaaaaca gaaaataaaa tgctcacatt gtgaggctaa ggtcaaaaga aatcagaaat
      661 gcatgagaat ttccaaatcc agggttaaga acaacataga ggaaaaaatt tctggtttaa
      721 gttgcaaaaa tgtgtttctt ttatacaata tactaacaaa tcataattaa ctgaaaaatg
      781 taacacttta tatgtaaagg ggagaaaact actccaccaa agatgccgca ttcatttaga
      841 gatcaaagaa atgtgtctgc caggcaacca agggctcatg gaaagctgtg gtttgtgtac
      901 aaatgctatt tgtctaatat tttgtgcgtt aatgactgtc ccattagcat cttcactaca
      961 cttattttca tagaaaggag aaacatgatt tatagagccc tttagtgaca agggtgagga
     1021 tcctatacat tatgttgctg gtttcctagt cttcagcaag aaagtgtagg agagaagcaa
     1081 aaaaagtcct gtttcaaccc ctgctcctgg atgtgacaag gaagaggagt tacccagctt
     1141 gaaacaaaag aaatcctaag tctgacacac aatgtcatgt ttaaattccc ctttctccaa
     1201 aatgtaaaat aaatgtgctt ccatcttcta aaatactatg ggactaaaca accttttgta
     1261 acgctaagga aatgccagta ttctcattga tttaggagag ggatgttctg gtcatagaaa
     1321 gatgctgtgt ctcagaaaca cttaaatact cttaagctag aaatagaggg gaaaagaatg
     1381 cctccctgca tctcccctca actgaagtcc tcctttctta gcctgatttc cgatgaaatg
     1441 tctgaatgcc tacagttatt tggccatcct caaaagtgca acttatcctg acgtctcaag
     1501 ggacggaaaa gttaccgaag tccaaggaat gagtcacttt gctcaaattt gatgagtaat
     1561 ctcaggtgtc atgaaaccca gtttcaaagg agaggggagg gggcgtcaga tctgcagacg
     1621 gaagcaggcc gctcccgatt ggatggcgag acctcgattt tcctaaaatt gcgtcattta
     1681 gaacccaatt gggtccagat gttatgggca tcgacgagtt accgtctcgg aaactctcaa
     1741 tcacgcaagc gaaaggagag gaggcggcta attaaatatt gagcagaaag tcgcgtgggg
     1801 agaatgtcac gtgggtttgg aggctcgagg aggctgggat aaataccgca aggcactcag
     1861 caggcgaaag agcGCGGCCG CGGATCCACC ATGGTGAGCA AGGGCGAGGA GCTGTTCACC
     1921 GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA GTTCAGCGTG
     1981 TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGCT GATCTGCACC
     2041 ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGGGCTA CGGCCTGCAG
     2101 TGCTTCGCCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC
     2161 GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA CAAGACCCGC
     2221 GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA GGGCATCGAC
     2281 TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA CAGCCACAAC
     2341 GTCTATATCA CCGCCGACAA GCAGAAGAAC GGCATCAAGG CCAACTTCAA GATCCGCCAC
     2401 AACATCGAGG ACGGCGGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC CCCCATCGGC
     2461 GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCT ACCAGTCCGC CCTGAGCAAA
     2521 GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGATC
     2581 ACTCTCGGCA TGGACGAGCT GTACAAGTAA ggcgcgccCT AGTcggatat ccgtcgaGCC
     2641 CCTCTCCCTC CCCCCCCCCT AACGTTACTG GCCGAAGCCG CTTGGAATAA GGCCGGTGTG
     2701 CGTTTGTCTA TATGTTATTT TCCACCATAT TGCCGTCTTT TGGCAATGTG AGGGCCCGGA
     2761 AACCTGGCCC TGTCTTCTTG ACGAGCATTC CTAGGGGTCT TTCCCCTCTC GCCAAAGGAA
     2821 TGCAAGGTCT GTTGAATGTC GTGAAGGAAG CAGTTCCTCT GGAAGCTTCT TGAAGACAAA
     2881 CAACGTCTGT AGCGACCCTT TGCAGGCAGC GGAACCCCCC ACCTGGCGAC AGGTGCCTCT
     2941 GCGGCCAAAA GCCACGTGTA TAAGATACAC CTGCAAAGGC GGCACAACCC CAGTGCCACG
     3001 TTGTGAGTTG GATAGTTGTG GAAAGAGTCA AATGGCTCTC CTCAAGCGTA TTCAACAAGG
     3061 GGCTGAAGGA TGCCCAGAAG GTACCCCATT GTATGGGATC TGATCTGGGG CCTCGGTACA
     3121 CATGCTTTAC ATGTGTTTAG TCGAGGTTAA AAAAACGTCT AGGCCCCCCG AACCACGGGG
     3181 ACGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCCA CAACCatgaa gctactgtct
     3241 tctatcgaac aagcatgcga tatttgccga cttaaaaagc tcaagtgctc caaagaaaaa
     3301 ccgaagtgcg ccaagtgtct gaagaacaac tgggagtgtc gctactctcc caaaaccaaa
     3361 aggtctccgc tgactagggc acatctgaca gaagtggaat caaggctaga aagactggaa
     3421 cagctatttc tactgatttt tcctcgagaa gaccttgaca tgattttgaa aatggattct
     3481 ttacaggata taaaagcatt gttaacagga ttatttgtac aagataatgt gaataaagat
     3541 gccgtcacag atagattggc ttcagtggag actgatatgc ctctaacatt gagacagcat
     3601 agaataagtg cgacatcatc atcggaagag agtagtaaca aaggtcaaag acagttgact
     3661 gtatcgccgg aattgggatc catggtgttt ccttctgggc agatctcaaa ccaggccctg
     3721 gccttagcac cgtcctctgc cccagtcctt gcccagacca tggtcccttc ctcagccatg
     3781 gtacctctgg ctcagccccc agctcctgcc ccagttctaa ccccgggtcc tccccagtcc
     3841 ctgtctgcac ctgttccaaa gagcacccag gctggggaag gcacgctgtc ggaagccctg
     3901 ctgcacctgc agtttgatgc tgatgaagac ttgggggcct tgcttggcaa cagcacagac
     3961 ccaggagtgt tcacagacct ggcatctgtg gacaactcag agtttcagca gctcctgaac
     4021 cagggtgtgt ccatgtctca ctccacagct gagcccatgc tgatggagta ccctgaagct
     4081 ataactcgcc tggtgacagg gtcccagagg ccccctgacc cagctcccac acccctgggg
     4141 acctcggggc ttcccaatgg tctctccgga gatgaagact tctcctccat tgcggacatg
     4201 gacttctctg ctctgctgag tcagatcagc tccagcggcc aatctagact gcagctcgac
     4261 ctcgaggggg ggcccggtac caggtaaGTT AACATCGAGG GATCAAGCTT ATCGATAATC
     4321 AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT GTTGCTCCTT
     4381 TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA TGCTATTGCT TCCCGTATGG
     4441 CTTTCATTTT CTCCTCCTTG TATAAATCCT GGTTGCTGTC TCTTTATGAG GAGTTGTGGC
     4501 CCGTTGTCAG GCAACGTGGC GTGGTGTGCA CTGTGTTTGC TGACGCAACC CCCACTGGTT
     4561 GGGGCATTGC CACCACCTGT CAGCTCCTTT CCGGGACTTT CGCTTTCCCC CTCCCTATTG
     4621 CCACGGCGGA ACTCATCGCC GCCTGCCTTG CCCGCTGCTG GACAGGGGCT CGGCTGTTGG
     4681 GCACTGACAA TTCCGTGGTG TTGTCGGGGA AGCTGACGTC CTTTCCATGG CTGCTCGCCT
     4741 GTGTTGCCAC CTGGATTCTG CGCGGGACGT CCTTCTGCTA CGTCCCTTCG GCCCTCAATC
     4801 CAGCGGACCT TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG CGTCTTCGCC
     4861 TTCGCCCTCA GACGAGTCGG ATCTCCCTTT GGGCCGCCTC CCCGCATCGt cgactaggga
     4921 taacagggta atggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     4981 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccagggtc tctctggtta
     5041 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     5101 taaagctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     5161 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     5221 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     5281 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     5341 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     5401 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     5461 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctgtgcggga
     5521 gaacggagtt ctattatgac tcaaatcagt ctccccaagc attcggggat cagagttttt
     5581 aaggataact tagtgtgtag ggggccagtg agttggagat gaaagcgtag ggagtcgaag
     5641 gtgtcctttt gcgccgagtc agttcctggg tgggggccac aagatcggat gagccagttt
     5701 atcaatccgg gggtgccagc tgatccatgg agtgcagggt ctgcaaaata tctcaagcac
     5761 tgattgatct taggttttac aatagtgatg ttaccccagg aacaatttgg ggaaggtcag
     5821 aatcttgtag cctgtagctg catgactcct aaaccataat ttcttttttg tttttttttt
     5881 tttatttttg agacagggtc tcactctgtc acctaggctg gagtgcagtg gtgcaatcac
     5941 agctcactgc agcctcaacg tcgtaagctc aagcgatcct cccacctcag cctgcctggt
     6001 agctgagact acaagcgacg ccccagttaa tttttgtatt tttggtagag gcagcgtttt
     6061 gccgtgtggc cctggctggt ctcgaactcc tgggctcaag tgatccagcc tcagcctccc
     6121 aaagtgctgg gacaaccggg gccagtcact gcacctggcc ctaaaccata atttctaatc
     6181 ttttggctaa tttgttagtc ctacaaaggc agtctagtcc ccaggcaaaa agggggtttg
     6241 tttcgggaaa gggctgttac tgtctttgtt tcaaactata aactaagttc ctcctaaact
     6301 tagttcggcc tacacccagg aatgaacaag gagagcttgg aggttagaag cacgatggaa
     6361 ttggttaggt cagatctctt tcactgtctg agttataatt ttgcaatggt ggttcaaaga
     6421 ctgcccgctt ctgacaccag tcgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     6481 gtttgcgtat tggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     6541 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     6601 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     6661 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     6721 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     6781 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     6841 ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc
     6901 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     6961 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     7021 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     7081 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     7141 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     7201 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     7261 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     7321 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     7381 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     7441 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     7501 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     7561 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     7621 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     7681 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     7741 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     7801 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     7861 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     7921 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     7981 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     8041 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     8101 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     8161 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     8221 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     8281 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     8341 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     8401 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     8461 aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaactgc ctcgcgcgtt
     8521 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     8581 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     8641 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtactgg cttaactatg
     8701 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     8761 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg
     8821 aagggcgatc ggtgcgggcc tcttcgctat tacgccagcg cggggaggca gagattgcag
     8881 taagctgaga tcgcagcact gcactccagc ctgggcgaca gagtaagact ctgtctcaaa
     8941 aataaaataa ataaatcaat cagatattcc aatcttttcc tttatttatt tatttatttt
     9001 ctattttgga aacacagtcc ttccttattc cagaattaca catatattct atttttcttt
     9061 atatgctcca gtttttttta gaccttcacc tgaaatgtgt gtatacaaaa tctaggccag
     9121 tccagcagag cctaaaggta aaaaataaaa taataaaaaa taaataaaat ctagctcact
     9181 ccttcacatc aaaatggaga tacagctgtt agcattaaat accaaataac ccatcttgtc
     9241 ctcaataatt ttaagcgcct ctctccacca catctaactc ctgtcaaagg catgtgcccc
     9301 ttccgggcgc tctgctgtgc tgccaaccaa ctggcatgtg gactctgcag ggtccctaac
     9361 tgccaagccc cacagtgtgc cctgaggctg ccccttcctt ctagcggctg cccccactcg
     9421 gctttgcttt ccctagtttc agttacttgc gttcagccaa ggtctgaaac taggtgcgca
     9481 cagagcggta agactgcgag agaaagagac cagctttaca gggggtttat cacagtgcac
     9541 cctgacagtc gtcagcctca cagggggttt atcacattgc accctgacag tcgtcagcct
     9601 cacagggggt ttatcacagt gcacccttac aatcattcca tttgattcac aattttttta
     9661 gtctctactg tgcctaactt gtaagttaaa tttgatcaga ggtgtgttcc cagaggggaa
     9721 aacagtatat acagggttca gtactatcgc atttcagggt ggcccgagag ctgcatccgg
     9781 agtatctaga tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     9841 cccaacgacc cccgcccatt gacgtcaata atgactgatg ttcccatagt aacgccaata
     9901 gggactttcc attgacgcta atgggagttt gttttggcac caaaatcaac gggactttcc
     9961 aaaatgtcgt aataaccccg ccccgttgac gcaaatgggc ggtaggcgtg tactctagaa
    10021 ggtctatata agcagagctc gtttagtgaa ccgtttatac tacttatctg gtctctctgg
    10081 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
    10141 caataaagct tgccttgagt gctcaaagta gtgtgtgccc gtctgttgtg tgactctggt
    10201 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
    10261 acagggactt gaaagcgaaa gtaaagccag aggagatctc tcgacgcagg actcggcttg
    10321 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
    10381 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cggtattaag cgggggagaa
    10441 ttagataaat gggaaaaaat tcggttaagg ccagggggaa agaaacaata taaactaaaa
    10501 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg ccttttagag
    10561 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
    10621 gaagaactta gatcattata taatacaata gcagtcctct attgtgtgca tcaaaggata
    10681 gatgtaaaag acaccaagga agccttagat aagatagagg aagagcaaaa caaaagtaag
    10741 aaaaaggcac agcaagcagc agctgacaca ggaaacaaca gccaggtcag ccaaaattac
    10801 cctatagtgc agaacctcca ggggcaaatg gtacatcagg ccatatcacc tagaacttta
    10861 aatgcatggg taaaagtagt agaagagaag gctttcagcc cagaagtaat acccatgttt
    10921 tcagcattat cagaaggagc caccccacaa gatttaaata ccatgctaaa cacagtgggg
    10981 ggacatcaag cagccatgca aatgttaaaa gagaccatca atgaggaagc tgcagaatgg
    11041 gatagattgc atccagtgca tgcagggcct attgcaccag gccagatgag agaaccaagg
    11101 ggaagtgaca tagcaggaac tactagcaaa ttaagagaac aatttggaaa taataaaaca
    11161 ataatcttta agcaatcctc aggaggggac ccagaaattg taacgcacag ttttaattgt
    11221 ggaggggaat ttttctactg taattcaaca caactgttta atagtacttg gtttaatagt
    11281 acttggagta ctgaagggtc aaataacact gaaggaagtg acacaatcac actcccatgc
    11341 agaataaaac aatttataaa catgtggcag gaagtaggaa aagcaatgta tgcccctccc
    11401 atcagtggac aaattagatg ttcatcaaat attactgggc tgctattaac aagagatggt
    11461 ggtaataaca acaatgggtc cgagatcttc agacctggag gaggcgatat gagggacaat
    11521 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
    11581 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
    11641 ttccttgggt tcttgggagc agcaggaagc actatgggcg cacggtcaat gacgctgacg
    11701 gtacaggcca gacaattatt gtctgatata gtgcagcagc agaacaattt gctgagggct
    11761 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaaaca gctccaggca
    11821 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
    11881 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
    11941 ctggaacaga tttggaataa catgacctgg atggagtggg acagagaaat taacaattac
    12001 acaAgcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
    12061 gaattattgg aattagataa atgggcttgc ggccggaatt cacaaatggc agtattcatc
    12121 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
    12181 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattactaa aaattcnaaa
    12241 attttcgggt ttattacagg gacagcagag atccactttg ggc 
//
