LOCUS       428_31__pTYF_sup       11430 bp ds-DNA     circular     07-AUG-2018
SOURCE      
  ORGANISM  
COMMENT     revamped version with oligos ATSceTyf 5 & 6
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     1023..1040
                     /locus_tag="TPH2 NotI Venus Fw"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          201..1020
                     /organism="Macaca mulatta"
                     /mol_type="genomic DNA"
                     /isolate="17573"
                     /db_xref="taxon:9544"
                     /chromosome="3"
                     /sex="female"
                     /country="USA: Southwest National Primate Research Center
                     at the Southwest Foundation for Biomedical Research, San
                     Antonio, TX"
                     /note="derived from Indian origin rhesus"
                     /locus_tag="derived from Indian origin rhesus"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3163..4023
                     /locus_tag="sequence checked"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            838..1020
                     /gene="TAC1"
                     /note="tachykinin, precursor 1; Derived by automated
                     computational analysis using gene prediction method:
                     BestRefSeq,Gnomon."
                     /db_xref="GeneID:697350"
                     /locus_tag="TAC1"
                     /ApEinfo_fwdcolor="#ff797d"
                     /ApEinfo_revcolor="#ff102f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2422..3385
                     /locus_tag="sequence checked(1)"
                     /ApEinfo_label="sequence checked"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     mRNA            838..1020
                     /gene="TAC1"
                     /product="tachykinin precursor 1, transcript variant X1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Gnomon. Supporting evidence
                     includes similarity to: 8 mRNAs, 45 ESTs, 4 Proteins, and
                     99% coverage of the annotated genomic feature by RNAseq
                     alignments, including 33 samples with support for all
                     annotated introns"
                     /transcript_id="XM_015134258.1"
                     /db_xref="GeneID:697350"
                     /locus_tag="tachykinin precursor 1, transcript variant X1"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1808..2795
                     /locus_tag="sequence checked(2)"
                     /ApEinfo_label="sequence checked"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     mRNA            879..1020
                     /gene="TAC1"
                     /product="tachykinin precursor 1, transcript variant X3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Gnomon. Supporting evidence
                     includes similarity to: 2 mRNAs, 15 ESTs, 2 Proteins, and
                     100% coverage of the annotated genomic feature by RNAseq
                     alignments, including 31 samples with support for all
                     annotated introns"
                     /transcript_id="XM_015134260.1"
                     /db_xref="GeneID:697350"
                     /locus_tag="tachykinin precursor 1, transcript variant X3"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    9448..9585
                     /vntifkey="21"
                     /locus_tag="HIV-1\Genomic\RNA\Packing\Signal\(Psi)"
                     /ApEinfo_fwdcolor="#cfcfcf"
                     /ApEinfo_revcolor="#cfcfcf"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     mRNA            881..1020
                     /gene="TAC1"
                     /product="tachykinin precursor 1, transcript variant X2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Gnomon. Supporting evidence
                     includes similarity to: 2 mRNAs, 21 ESTs, 3 Proteins, and
                     100% coverage of the annotated genomic feature by RNAseq
                     alignments, including 33 samples with support for all
                     annotated introns"
                     /transcript_id="XM_015134259.1"
                     /db_xref="GeneID:697350"
                     /locus_tag="tachykinin precursor 1, transcript variant X2"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    186..187
                     /vntifkey="31"
                     /note="GAL-4 binding sites"
                     /locus_tag="GAL4-BS"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     mRNA            899..1020
                     /gene="TAC1"
                     /product="tachykinin precursor 1"
                     /inference="similar to RNA sequence, mRNA (same
                     species):RefSeq:NM_001257461.2"
                     /exception="annotated by transcript or proteomic data"
                     /note="The RefSeq transcript has 5 substitutions compared
                     to this genomic sequence; Derived by automated
                     computational analysis using gene prediction method:
                     BestRefSeq."
                     /transcript_id="NM_001257461.2"
                     /db_xref="GeneID:697350"
                     /locus_tag="tachykinin precursor 1"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          1785..2264
                     /organism="pIRES2-EGFP"
                     /mol_type="other DNA"
                     /locus_tag="source:pIRES2-EGFP"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    36..185
                     /vntifkey="31"
                     /note="GAL-4 binding sites"
                     /locus_tag="GAL4-BS(1)"
                     /ApEinfo_label="GAL4-BS"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     186..194
                     /locus_tag="156 invPCR Rv"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_signal     3466..4041
                     /locus_tag="WPRE"
                     /ApEinfo_fwdcolor="#00ff40"
                     /ApEinfo_revcolor="#00ff40"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    9217..9396
                     /vntifkey="21"
                     /locus_tag="5'\LRT"
                     /ApEinfo_fwdcolor="#cfcfcf"
                     /ApEinfo_revcolor="#cfcfcf"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1785..2264
                     /locus_tag="IRES"
                     /ApEinfo_fwdcolor="#c0c0c0"
                     /ApEinfo_revcolor="#c0c0c0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1001..1020
                     /locus_tag="primer 41 R"
                     /ApEinfo_fwdcolor="#408080"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    10789..10993
                     /vntifkey="21"
                     /locus_tag="RRE"
                     /ApEinfo_fwdcolor="#cfcfcf"
                     /ApEinfo_revcolor="#cfcfcf"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    201..225
                     /locus_tag="primer 40 F2"
                     /ApEinfo_fwdcolor="#ff0080"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             2361..2372
                     /gene="EGFP"
                     /note="ORF frame 3"
                     /translation="MATTMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATY
                     GKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERT
                     IFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMAD
                     KQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNE
                     KRDHMVLLEFVTAAGITLGMDELYK*"
                     /locus_tag="EGFP"
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="#0000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             6585..7445
                     /vntifkey="4"
                     /locus_tag="Ampicillin\Resistance\Gene"
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="#0000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    11273..11391
                     /vntifkey="21"
                     /locus_tag="cPPT-DNA\FLAP"
                     /ApEinfo_fwdcolor="#cfcfcf"
                     /ApEinfo_revcolor="#cfcfcf"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4062..4079
                     /vntifkey="21"
                     /locus_tag="I-SceI"
                     /ApEinfo_fwdcolor="#00ff40"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4253..4510
                     /vntifkey="21"
                     /locus_tag="bGHpA"
                     /ApEinfo_fwdcolor="#cfcfcf"
                     /ApEinfo_revcolor="#cfcfcf"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5..22
                     /vntifkey="21"
                     /locus_tag="I-SceI(1)"
                     /ApEinfo_label="I-SceI"
                     /ApEinfo_fwdcolor="#00ff40"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    11317..11336
                     /locus_tag="pTYF out Fw1"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5..5
                     /locus_tag="T?"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4042..4056
                     /locus_tag="WPRE(1)"
                     /ApEinfo_label="WPRE"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     exon            2373..3434
                     /vntifkey="61"
                     /locus_tag="Gal4p65"
                     /ApEinfo_fwdcolor="#ff80c0"
                     /ApEinfo_revcolor="#ff80c0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          2266..2372
                     /organism="pIRES2-EGFP"
                     /mol_type="other DNA"
                     /locus_tag="source:pIRES2-EGFP(1)"
                     /ApEinfo_label="source:pIRES2-EGFP"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2266..2369
                     /locus_tag="IRES(1)"
                     /ApEinfo_label="IRES"
                     /ApEinfo_fwdcolor="#c0c0c0"
                     /ApEinfo_revcolor="#c0c0c0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2265..2265
                     /locus_tag="New Feature"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4176..4254
                     /locus_tag="LTR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     170..185
                     /locus_tag="156 invPCR Rv(1)"
                     /ApEinfo_label="156 invPCR Rv"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     1773..1785
                     /locus_tag="156 invPCR Fw +AscI"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             1038..1757
                     /locus_tag="Venus"
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="#0000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     1738..1772
                     /locus_tag="Venus AscI Rv"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1023..1735
                     /locus_tag="sequence verified"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    195..195
                     /locus_tag="Mlu I"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1023..1028
                     /locus_tag="Not I"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 ggcctaggga taacagggta atagatcttg ctagctctta tcatgtctgg atccaagctt
       61 gcatgcctgc aggtcggagt actgtcctcc gagcggagta ctgtcctccg agcggagtac
      121 tgtcctccga gcggagtact gtcctccgag cggagtactg tcctccgagc ggagactcta
      181 gagggtcccg ggatACGCGT cagcaagaaa gtgtaggaga gaagcaaaaa aagtcctgtt
      241 ccaacccctg ctcctggatg tgacaaggaa gaggagttac ccagcttgaa acaaaagaaa
      301 tcctaagtct gacacacaat gtcatgttta aattcccctt tctccaaaat gtaaaataaa
      361 tgtgcttcca tcttctaaaa tactatggga ctaaacaacc ttttgtaacg ctaaggaaat
      421 gccagtattc tcattgattt aggagaggga tgttctggtc atagaaagat gctgtgtctc
      481 agaaacactt aaatactctt aagctagaaa tagaggggaa aagaatgcct ccctgcatct
      541 cccctcaact gaagtcctcc tttcttagcc tgatttccga tgaaatgtct gaatgcctac
      601 agttatttgg ccatcctcaa aagtgcaact tatcctgacg tctcaaggga cggaaaagtt
      661 accgaagtcc aaggaatgag tcactttgct caaatttgat gagtaatctc aggtgtcatg
      721 aaacccagtt tcaaaggaga ggggaggggg cgtcagatct gcagacggaa gcaggccgct
      781 cccgattgga tggcgagacc tcgattttcc taaaattgcg tcatttagaa cccaattggg
      841 tccagatgtt atgggcatcg acgagttacc gtctcggaaa ctctcaatca cgcaagcgaa
      901 aggagaggag gcggctaatt aaatattgag cagaaagtcg cgtggggaga atgtcacgtg
      961 ggtttggagg ctcgaggagg ctgggataaa taccgcaagg cactcagcag gcgaaagagc
     1021 GCGGCCGCGG ATCCACCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA
     1081 TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG
     1141 AGGGCGATGC CACCTACGGC AAGCTGACCC TGAAGCTGAT CTGCACCACC GGCAAGCTGC
     1201 CCGTGCCCTG GCCCACCCTC GTGACCACCC TGGGCTACGG CCTGCAGTGC TTCGCCCGCT
     1261 ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC
     1321 AGGAGCGCAC CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT
     1381 TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG
     1441 GCAACATCCT GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC TATATCACCG
     1501 CCGACAAGCA GAAGAACGGC ATCAAGGCCA ACTTCAAGAT CCGCCACAAC ATCGAGGACG
     1561 GCGGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC
     1621 TGCTGCCCGA CAACCACTAC CTGAGCTACC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA
     1681 AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG
     1741 ACGAGCTGTA CAAGTAAggc gcgccCTAGT cggatatccg tcgaGCCCCT CTCCCTCCCC
     1801 CCCCCCTAAC GTTACTGGCC GAAGCCGCTT GGAATAAGGC CGGTGTGCGT TTGTCTATAT
     1861 GTTATTTTCC ACCATATTGC CGTCTTTTGG CAATGTGAGG GCCCGGAAAC CTGGCCCTGT
     1921 CTTCTTGACG AGCATTCCTA GGGGTCTTTC CCCTCTCGCC AAAGGAATGC AAGGTCTGTT
     1981 GAATGTCGTG AAGGAAGCAG TTCCTCTGGA AGCTTCTTGA AGACAAACAA CGTCTGTAGC
     2041 GACCCTTTGC AGGCAGCGGA ACCCCCCACC TGGCGACAGG TGCCTCTGCG GCCAAAAGCC
     2101 ACGTGTATAA GATACACCTG CAAAGGCGGC ACAACCCCAG TGCCACGTTG TGAGTTGGAT
     2161 AGTTGTGGAA AGAGTCAAAT GGCTCTCCTC AAGCGTATTC AACAAGGGGC TGAAGGATGC
     2221 CCAGAAGGTA CCCCATTGTA TGGGATCTGA TCTGGGGCCT CGGTACACAT GCTTTACATG
     2281 TGTTTAGTCG AGGTTAAAAA AACGTCTAGG CCCCCCGAAC CACGGGGACG TGGTTTTCCT
     2341 TTGAAAAACA CGATGATAAT ATGGCCACAA CCatgaagct actgtcttct atcgaacaag
     2401 catgcgatat ttgccgactt aaaaagctca agtgctccaa agaaaaaccg aagtgcgcca
     2461 agtgtctgaa gaacaactgg gagtgtcgct actctcccaa aaccaaaagg tctccgctga
     2521 ctagggcaca tctgacagaa gtggaatcaa ggctagaaag actggaacag ctatttctac
     2581 tgatttttcc tcgagaagac cttgacatga ttttgaaaat ggattcttta caggatataa
     2641 aagcattgtt aacaggatta tttgtacaag ataatgtgaa taaagatgcc gtcacagata
     2701 gattggcttc agtggagact gatatgcctc taacattgag acagcataga ataagtgcga
     2761 catcatcatc ggaagagagt agtaacaaag gtcaaagaca gttgactgta tcgccggaat
     2821 tgggatccat ggtgtttcct tctgggcaga tctcaaacca ggccctggcc ttagcaccgt
     2881 cctctgcccc agtccttgcc cagaccatgg tcccttcctc agccatggta cctctggctc
     2941 agcccccagc tcctgcccca gttctaaccc cgggtcctcc ccagtccctg tctgcacctg
     3001 ttccaaagag cacccaggct ggggaaggca cgctgtcgga agccctgctg cacctgcagt
     3061 ttgatgctga tgaagacttg ggggccttgc ttggcaacag cacagaccca ggagtgttca
     3121 cagacctggc atctgtggac aactcagagt ttcagcagct cctgaaccag ggtgtgtcca
     3181 tgtctcactc cacagctgag cccatgctga tggagtaccc tgaagctata actcgcctgg
     3241 tgacagggtc ccagaggccc cctgacccag ctcccacacc cctggggacc tcggggcttc
     3301 ccaatggtct ctccggagat gaagacttct cctccattgc ggacatggac ttctctgctc
     3361 tgctgagtca gatcagctcc agcggccaat ctagactgca gctcgacctc gagggggggc
     3421 ccggtaccag gtaaGTTAAC ATCGAGGGAT CAAGCTTATC GATAATCAAC CTCTGGATTA
     3481 CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA CGCTATGTGG
     3541 ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC CGTATGGCTT TCATTTTCTC
     3601 CTCCTTGTAT AAATCCTGGT TGCTGTCTCT TTATGAGGAG TTGTGGCCCG TTGTCAGGCA
     3661 ACGTGGCGTG GTGTGCACTG TGTTTGCTGA CGCAACCCCC ACTGGTTGGG GCATTGCCAC
     3721 CACCTGTCAG CTCCTTTCCG GGACTTTCGC TTTCCCCCTC CCTATTGCCA CGGCGGAACT
     3781 CATCGCCGCC TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA CTGACAATTC
     3841 CGTGGTGTTG TCGGGGAAGC TGACGTCCTT TCCATGGCTG CTCGCCTGTG TTGCCACCTG
     3901 GATTCTGCGC GGGACGTCCT TCTGCTACGT CCCTTCGGCC CTCAATCCAG CGGACCTTCC
     3961 TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT CTTCGCCTTC GCCCTCAGAC
     4021 GAGTCGGATC TCCCTTTGGG CCGCCTCCCC GCATCGtcga ctagggataa cagggtaatg
     4081 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     4141 aaggggggac tggaagggct aattcactcc cagggtctct ctggttagac cagatctgag
     4201 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agctcgactg
     4261 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     4321 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     4381 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     4441 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     4501 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
     4561 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     4621 tcgctttctt cccttccttt ctcgccacgt tcgccggctg tgcgggagaa cggagttcta
     4681 ttatgactca aatcagtctc cccaagcatt cggggatcag agtttttaag gataacttag
     4741 tgtgtagggg gccagtgagt tggagatgaa agcgtaggga gtcgaaggtg tccttttgcg
     4801 ccgagtcagt tcctgggtgg gggccacaag atcggatgag ccagtttatc aatccggggg
     4861 tgccagctga tccatggagt gcagggtctg caaaatatct caagcactga ttgatcttag
     4921 gttttacaat agtgatgtta ccccaggaac aatttgggga aggtcagaat cttgtagcct
     4981 gtagctgcat gactcctaaa ccataatttc ttttttgttt tttttttttt atttttgaga
     5041 cagggtctca ctctgtcacc taggctggag tgcagtggtg caatcacagc tcactgcagc
     5101 ctcaacgtcg taagctcaag cgatcctccc acctcagcct gcctggtagc tgagactaca
     5161 agcgacgccc cagttaattt ttgtattttt ggtagaggca gcgttttgcc gtgtggccct
     5221 ggctggtctc gaactcctgg gctcaagtga tccagcctca gcctcccaaa gtgctgggac
     5281 aaccggggcc agtcactgca cctggcccta aaccataatt tctaatcttt tggctaattt
     5341 gttagtccta caaaggcagt ctagtcccca ggcaaaaagg gggtttgttt cgggaaaggg
     5401 ctgttactgt ctttgtttca aactataaac taagttcctc ctaaacttag ttcggcctac
     5461 acccaggaat gaacaaggag agcttggagg ttagaagcac gatggaattg gttaggtcag
     5521 atctctttca ctgtctgagt tataattttg caatggtggt tcaaagactg cccgcttctg
     5581 acaccagtcg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     5641 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     5701 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     5761 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     5821 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     5881 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     5941 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     6001 ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     6061 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     6121 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     6181 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     6241 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     6301 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     6361 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     6421 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     6481 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     6541 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     6601 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     6661 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     6721 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     6781 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     6841 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     6901 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     6961 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     7021 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     7081 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     7141 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     7201 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     7261 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     7321 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     7381 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     7441 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     7501 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     7561 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     7621 aggcgtatca cgaggccctt tcgtcttcaa gaactgcctc gcgcgtttcg gtgatgacgg
     7681 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     7741 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
     7801 catgacccag tcacgtagcg atagcggagt gtactggctt aactatgcgg catcagagca
     7861 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     7921 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     7981 gcgggcctct tcgctattac gccagcgcgg ggaggcagag attgcagtaa gctgagatcg
     8041 cagcactgca ctccagcctg ggcgacagag taagactctg tctcaaaaat aaaataaata
     8101 aatcaatcag atattccaat cttttccttt atttatttat ttattttcta ttttggaaac
     8161 acagtccttc cttattccag aattacacat atattctatt tttctttata tgctccagtt
     8221 ttttttagac cttcacctga aatgtgtgta tacaaaatct aggccagtcc agcagagcct
     8281 aaaggtaaaa aataaaataa taaaaaataa ataaaatcta gctcactcct tcacatcaaa
     8341 atggagatac agctgttagc attaaatacc aaataaccca tcttgtcctc aataatttta
     8401 agcgcctctc tccaccacat ctaactcctg tcaaaggcat gtgccccttc cgggcgctct
     8461 gctgtgctgc caaccaactg gcatgtggac tctgcagggt ccctaactgc caagccccac
     8521 agtgtgccct gaggctgccc cttccttcta gcggctgccc ccactcggct ttgctttccc
     8581 tagtttcagt tacttgcgtt cagccaaggt ctgaaactag gtgcgcacag agcggtaaga
     8641 ctgcgagaga aagagaccag ctttacaggg ggtttatcac agtgcaccct gacagtcgtc
     8701 agcctcacag ggggtttatc acattgcacc ctgacagtcg tcagcctcac agggggttta
     8761 tcacagtgca cccttacaat cattccattt gattcacaat ttttttagtc tctactgtgc
     8821 ctaacttgta agttaaattt gatcagaggt gtgttcccag aggggaaaac agtatataca
     8881 gggttcagta ctatcgcatt tcagggtggc ccgagagctg catccggagt atctagatgg
     8941 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     9001 gcccattgac gtcaataatg actgatgttc ccatagtaac gccaataggg actttccatt
     9061 gacgctaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaat
     9121 aaccccgccc cgttgacgca aatgggcggt aggcgtgtac tctagaaggt ctatataagc
     9181 agagctcgtt tagtgaaccg tttatactac ttatctggtc tctctggtta gaccagatct
     9241 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     9301 cttgagtgct caaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     9361 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     9421 agcgaaagta aagccagagg agatctctcg acgcaggact cggcttgctg aagcgcgcac
     9481 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     9541 gaaggagaga gatgggtgcg agagcgtcgg tattaagcgg gggagaatta gataaatggg
     9601 aaaaaattcg gttaaggcca gggggaaaga aacaatataa actaaaacat atagtatggg
     9661 caagcaggga gctagaacga ttcgcagtta atcctggcct tttagagaca tcagaaggct
     9721 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     9781 cattatataa tacaatagca gtcctctatt gtgtgcatca aaggatagat gtaaaagaca
     9841 ccaaggaagc cttagataag atagaggaag agcaaaacaa aagtaagaaa aaggcacagc
     9901 aagcagcagc tgacacagga aacaacagcc aggtcagcca aaattaccct atagtgcaga
     9961 acctccaggg gcaaatggta catcaggcca tatcacctag aactttaaat gcatgggtaa
    10021 aagtagtaga agagaaggct ttcagcccag aagtaatacc catgttttca gcattatcag
    10081 aaggagccac cccacaagat ttaaatacca tgctaaacac agtgggggga catcaagcag
    10141 ccatgcaaat gttaaaagag accatcaatg aggaagctgc agaatgggat agattgcatc
    10201 cagtgcatgc agggcctatt gcaccaggcc agatgagaga accaagggga agtgacatag
    10261 caggaactac tagcaaatta agagaacaat ttggaaataa taaaacaata atctttaagc
    10321 aatcctcagg aggggaccca gaaattgtaa cgcacagttt taattgtgga ggggaatttt
    10381 tctactgtaa ttcaacacaa ctgtttaata gtacttggtt taatagtact tggagtactg
    10441 aagggtcaaa taacactgaa ggaagtgaca caatcacact cccatgcaga ataaaacaat
    10501 ttataaacat gtggcaggaa gtaggaaaag caatgtatgc ccctcccatc agtggacaaa
    10561 ttagatgttc atcaaatatt actgggctgc tattaacaag agatggtggt aataacaaca
    10621 atgggtccga gatcttcaga cctggaggag gcgatatgag ggacaattgg agaagtgaat
    10681 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
    10741 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
    10801 tgggagcagc aggaagcact atgggcgcac ggtcaatgac gctgacggta caggccagac
    10861 aattattgtc tgatatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
    10921 agcatctgtt gcaactcaca gtctggggca tcaaacagct ccaggcaaga atcctggctg
    10981 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
    11041 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
    11101 ggaataacat gacctggatg gagtgggaca gagaaattaa caattacaca Agcttaatac
    11161 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
    11221 tagataaatg ggcttgcggc cggaattcac aaatggcagt attcatccac aattttaaaa
    11281 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
    11341 acatacaaac taaagaatta caaaaacaaa ttactaaaaa ttcnaaaatt ttcgggttta
    11401 ttacagggac agcagagatc cactttgggc   
//
