LOCUS       Exported                7195 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 7195)
  AUTHORS   Conklin Lab
  TITLE     Direct Submission
  JOURNAL   Exported Dec 19, 2018 from SnapGene 4.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7195
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     promoter        7..1718
                     /locus_tag="CAGGS Promoter"
                     /label=CAGGS Promoter
                     /note="color: #00ffff"
     primer_bind     1678..1697
                     /locus_tag="pCAGGS-Forward Primer"
                     /label=pCAGGS-Forward Primer
                     /note="color: #0000ff; direction: RIGHT"
     region          1744..1753
                     /locus_tag="Region"
                     /label=Region
                     /note="color: #ffc0cb"
     misc_feature    1754..1822
                     /locus_tag="3xFLAG"
                     /label=3xFLAG
                     /note="3xFLAG"
                     /note="color: #5c5c5c"
     misc_feature    1829..3732
                     /locus_tag="Boris cDNA"
                     /label=Boris cDNA
                     /note="color: #bbbbff"
     misc_feature    2600..2662
                     /locus_tag="BORIS_ZF1"
                     /label=BORIS_ZF1
                     /note="color: #ff0080"
     misc_feature    2684..2746
                     /locus_tag="BORIS_ZF2"
                     /label=BORIS_ZF2
                     /note="color: #ff0080"
     misc_feature    2768..2833
                     /locus_tag="BORIS_ZF3"
                     /label=BORIS_ZF3
                     /note="color: #ff0080"
     misc_feature    2855..2917
                     /locus_tag="BORIS_ZF4"
                     /label=BORIS_ZF4
                     /note="color: #ff0080"
     misc_feature    2939..3001
                     /locus_tag="BORIS_ZF5"
                     /label=BORIS_ZF5
                     /note="color: #ff0080"
     misc_feature    3023..3088
                     /locus_tag="BORIS_ZF6"
                     /label=BORIS_ZF6
                     /note="color: #ff0080"
     misc_feature    3113..3178
                     /locus_tag="BORIS_ZF7"
                     /label=BORIS_ZF7
                     /note="color: #ff0080"
     misc_feature    3203..3265
                     /locus_tag="BORIS_ZF8"
                     /label=BORIS_ZF8
                     /note="color: #ff0080"
     misc_feature    3287..3349
                     /locus_tag="BORIS_ZF9"
                     /label=BORIS_ZF9
                     /note="color: #ff0080"
     misc_feature    3371..3436
                     /locus_tag="BORIS_ZF10"
                     /label=BORIS_ZF10
                     /note="color: #ff0080"
     misc_feature    3467..3541
                     /locus_tag="BORIS_ZF11"
                     /label=BORIS_ZF11
                     /note="color: #ff0080"
     primer_bind     3721..3774
                     /label=ks_clo_CTCF_linker_eGFP_F_031
                     /note="color: black; sequence: 
                     AGCATGATGGACCGGCTTAAGGGGGGAGCAGGAGGCGTGAGCAAGGGCGAGGAGCT"
     misc_feature    3755..4461
                     /locus_tag="eGFP"
                     /label=eGFP
                     /note="color: #42ff42"
     primer_bind     complement(4449..4489)
                     /label=ks_clo_pA_STOP_eGFP_R_032
                     /note="color: black; sequence: 
                     ACCTGAGCTAGCTTATCGCGACTTGTACAGCTCGTCCATGC"
     misc_feature    4462..4468
                     /locus_tag="eGFP(1)"
                     /label=eGFP
                     /label=eGFP(1)
                     /note="color: #42ff42"
     misc_feature    4475..4477
                     /locus_tag="STOP"
                     /label=STOP
                     /note="color: #ff8080"
     polyA_site      4484..5013
                     /locus_tag="Rabbit Globin pA"
                     /label=Rabbit Globin pA
                     /note="color: #800080"
     polyA_site      4484..5007
                     /locus_tag="Rabbit globin polyA"
                     /label=Rabbit globin polyA
                     /note="color: #800080"
     primer_bind     complement(5031..5051)
                     /locus_tag="M13-rev"
                     /label=M13-rev
                     /note="color: #00ff00; direction: LEFT"
     misc_binding    complement(5057..5079)
                     /locus_tag="LacO"
                     /label=LacO
                     /note="color: #6495ed; direction: LEFT"
     rep_origin      complement(5427..6109)
                     /direction=LEFT
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
                     /note="color: #808080"
     CDS             complement(6207..6866)
                     /locus_tag="AmpR"
                     /label=AmpR
                     /note="color: #ffff00; direction: LEFT"
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcctcgag aattctcacg
     1741 cgtgaattcc accatggact ataaggacca cgacggagac tacaaggatc atgatattga
     1801 ttacaaagac gatgacgata agaccggtgc tgccgctgag gtccctgtcc cttctgggta
     1861 cttcacccag atcaaagagc agaagttgaa gcctggagac ctagaggagg agaaagagga
     1921 ggacggggta caaagagtgg aagcccagga gggagttgtc aaggaggtgg aggccgagaa
     1981 cagttgcctg cttctggagg ccagggcccc ggtggagagc gacaggcgga tcctgaccct
     2041 gcaaacggtg cacctggagt cccaggatgt gcacctacag gggctgggat ggctgagcgt
     2101 gccacactct gaggagcttt cagggacggt accagaggcg gaaggcatac tgcagttgcc
     2161 atccgtgctg tggctcgacc cagagcccca gctcagcctt cagcattgcg tgacggtcag
     2221 catcccggaa gagctgtacc caccagagga gctgcagcgg atacattttc acctgctgag
     2281 agagaatgtg ctaatggccg aggagaaccc agagttaaca ccagacttgg acgaaagcac
     2341 agccctgaaa aagcccgaag aagatgaaaa ggaccagctc ccgccccagg gagagacaga
     2401 caagagagaa gagaggttgc tccttctgga aatgaaacca aaagagggaa aagacgacga
     2461 aattgtcctg accatttccc atctaagcct cgaagaacag caagatccac cagcggccaa
     2521 tcagacaagt gtgccgggag ccaaagccgc aaaaccaaaa cggcggaggc agaccaaggg
     2581 aaagcctcag agctttcagt gtgacacctg cccgttcact tcctccaagc tctcaacttt
     2641 caatcgtcac atcaaaattc acagcaatga gaggccacac ctgtgtcacc tgtgcctgaa
     2701 ggccttccgg actgtcactc ttcttaggaa ccatgtgaac acccacacag gaaccaggcc
     2761 ccacaagtgc agggactgcg acatggcgtt tgtcaccagt ggagaactcg tccggcacag
     2821 gcgttacaaa cacacttatg agaagccctt caagtgctcc ctgtgcaagt acgccagcgt
     2881 cgaggcaagc aagatgaagc gtcacatccg ctcacacacg ggtgagcgtc ccttccagtg
     2941 ttgccagtgt gcttacgcca gcagggactc ctacaagctg aagcgccaca tgaggacaca
     3001 ctcaggtgag aagccgtatg aatgtcccac ctgtcacgtc cggttcaccc agagcgggac
     3061 catgaaaatc catatagcac agaagcacgg agagaatgtg cccaaatacg agtgtcccca
     3121 ctgtgccacc atcatcgcga ggaagagcga cctgcgtgtc catctgcgta acctgcacag
     3181 ccagagcccg gaggagatga agtgccgata ctgtcccgct ggcttccatg agcgctatgc
     3241 cctcattcag caccagagga cccacaagaa cgagaagaag ttcaagtgca agcagtgcga
     3301 ttacgcgtgc aagcaggagc gatgcttgaa ggcgcacatg cgcatgcaca caggagagaa
     3361 gcccttctcc tgcctggcct gcaacaagca cttccgacag aagcagctac tgaccgtgca
     3421 cctgaggaag taccatgacc cgaacttcgt ccccaatctg cacctgtgcc tcaagtgtga
     3481 taaacgtttc tcccgctgga gtaacctgca gagacacaga aagaagtgtg acccggagca
     3541 tgagacgtta gcccccaaca aggacaggag accagtgaca aggacacagg cctcggaggg
     3601 agaagcagga cacaaggaag gggagcctca gtgccctggg gagcaggctc tgggccacca
     3661 aggagaagca gcggggagcc agagcccaga ccacggcctt acctgcgaga taatctttaa
     3721 catgatggat aagcttaagg ggggagcagg aggcgtgagc aagggcgagg agctgttcac
     3781 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     3841 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     3901 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
     3961 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     4021 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     4081 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     4141 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     4201 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     4261 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     4321 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     4381 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     4441 cactctcggc atggacgagc tgtacaagtc gcgataagct agctcaggtg caggctgcct
     4501 atcagaaggt ggtggctggt gtggccaatg ccctggctca caaataccac tgagatcttt
     4561 ttccctctgc caaaaattat ggggacatca tgaagcccct tgagcatctg acttctggct
     4621 aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg
     4681 gaaggacata tgggagggca aatcatttaa aacatcagaa tgagtatttg gtttagagtt
     4741 tggcaacata tgccatatgc tggctgccat gaacaaaggt ggctataaag aggtcatcag
     4801 tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt
     4861 tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc
     4921 ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc
     4981 cctcttctct tatgaagatc cctcgacctg cagcccaagc ttggcgtaat catggtcata
     5041 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     5101 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     5161 ctcactgccc gctttccagt cgggaaacct gtcgtgccag cggatccgct gcattaatga
     5221 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     5281 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     5341 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     5401 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     5461 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     5521 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     5581 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa
     5641 tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     5701 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     5761 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     5821 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     5881 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     5941 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     6001 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     6061 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     6121 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     6181 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     6241 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     6301 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     6361 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     6421 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     6481 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     6541 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     6601 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     6661 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     6721 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     6781 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     6841 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     6901 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     6961 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     7021 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     7081 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     7141 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctg
//
