LOCUS       Exported                8401 bp ds-DNA     circular SYN 26-JUN-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 8401)
  AUTHORS   MIT
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jun 26, 2020 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8401
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     protein_bind    98..117
                     /label=BACfor
     misc_feature    308..781
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             complement(854..1825)
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     CDS             complement(1825..3000)
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     misc_feature    3326..3576
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(3579..4334)
                     /codon_start=1
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     rep_origin      4379..4644
                     /label=oriS
     CDS             complement(4870..5730)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAISMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5731..5835)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    complement(5870..5925)
                     /label=T7 UUCG terminator
     promoter        5944..5962
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        5969..5997
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     CDS             6036..6410
                     /codon_start=1
                     /transl_table=11
                     /gene="rpsL"
                     /gene_synonym="asuB;ECK3329;JW3304;strA"
                     /locus_tag="b3342"
                     /product="30S ribosomal subunit protein S12"
                     /function="structural component; Ribosomal proteins - 
                     synthesis, modification"
                     /label=rpsL
                     /note="GO_component: GO:0005737 - cytoplasm; GO_function: 
                     GO:0003735 - structural constituent of ribosome; 
                     GO_process: GO:0006412 - translation"
                     /db_xref="GI:1789740"
                     /db_xref="ASAP:ABE-0010919"
                     /db_xref="UniProtKB/Swiss-Prot:P0A7S3"
                     /db_xref="EcoGene:EG10911"
                     /protein_id="AAC76367.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKKP
                     NSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGALDC
                     SGVKDRKQARSKYGVKRPKA"
     misc_feature    6460..6515
                     /label=T7 UUCG terminator
     misc_feature    6522..6577
                     /label=T7 UUCG terminator
     misc_feature    6584..6639
                     /label=T7 UUCG terminator
     misc_feature    6646..6701
                     /label=T7 UUCG terminator
     misc_feature    6708..6763
                     /label=T7 UUCG terminator
     misc_feature    6786..6841
                     /label=T7 UUCG terminator
     misc_feature    6848..6903
                     /label=T7 UUCG terminator
     misc_feature    6910..6965
                     /label=T7 UUCG terminator
     misc_feature    6972..7027
                     /label=T7 UUCG terminator
     misc_feature    7034..7089
                     /label=T7 UUCG terminator
     promoter        7108..7136
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             7182..8384
                     /codon_start=1
                     /gene="tetA from transposon Tn10"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="NSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFGVL
                     LALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRLLSG
                     ITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSPFFIA
                     ALLNIVTFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYFSAQLI
                     GQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKTAVLLGF
                     IADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGALQGLLVSL
                     TNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQAQGSKQETS
                     A"
ORIGIN
        1 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
       61 ttagacgtca ggtggcactt ttcggggaaa tgtgcccggt taacgtgccg gcacggcctg
      121 ggtaaccagg tattttgtcc acataaccgt gcgcaaaatg ttgtggataa gcaggacaca
      181 gcagcaatcc acagcaggca tacaaccgca caccgaggtt actccgttct acaggttacg
      241 acgacatgtc aatacttgcc cttgacaggc attgatggaa tcgtagtctc acgctgatag
      301 tctgatcgac aatacaagtg ggaccgtggt cccagaccga taatcagacc gacaacacga
      361 gtgggatcgt ggtcccagac taataatcag accgacgata cgagtgggac cgtggtccca
      421 gactaataat cagaccgacg atacgagtgg gaccgtggtt ccagactaat aatcagaccg
      481 acgatacgag tgggaccgtg gtcccagact aataatcaga ccgacgatac gagtgggacc
      541 atggtcccag actaataatc agaccgacga tacgagtggg accgtggtcc cagtctgatt
      601 atcagaccga cgatacgagt gggaccgtgg tcccagacta ataatcagac cgacgatacg
      661 agtgggaccg tggtcccaga ctaataatca gaccgacgat acgagtggga ccgtggtccc
      721 agtctgatta tcagaccgac gatacaagtg gaacagtggg cccagagaga atattcaggc
      781 cagttatgct ttctggcctg taacaaagga cattaagtaa agacagataa acgtagacta
      841 aaacgtggtc gcatcagggt gctggctttt caagttcctt aagaatggcc tcaattttct
      901 ctatacactc agttggaaca cgagacctgt ccaggttaag caccatttta tcgcccttat
      961 acaatactgt cgctccagga gcaaactgat gtcgtgagct taaactagtt cttgatgcag
     1021 atgacgtttt aagcacagaa gttaaaagag tgataacttc ttcagcttca aatatcaccc
     1081 cagctttttt ctgctcatga aggttagatg cctgctgctt aagtaattcc tctttatctg
     1141 taaaggcttt ttgaagtgca tcacctgacc gggcagatag ttcaccgggg tgagaaaaaa
     1201 gagcaacaac tgatttaggc aatttggcgg tgttgataca gcgggtaata atcttacgtg
     1261 aaatattttc cgcatcagcc agcgcagaaa tatttccagc aaattcattc tgcaatcggc
     1321 ttgcataacg ctgaccacgt tcataagcac ttgttgggcg ataatcgtta cccaatctgg
     1381 ataatgcagc catctgctca tcatccagct cgccaaccag aacacgataa tcactttcgg
     1441 taagtgcagc agctttacga cggcgactcc catcggcaat ttctatgaca ccagatactc
     1501 ttcgaccgaa cgccggtgtc tgttgaccag tcagtagaaa agaagggatg agatcatcca
     1561 gtgcgtcctc agtaagcagc tcctggtcac gttcattacc tgaccatacc cgagaggtct
     1621 tctcaacact atcaccccgg agcacttcaa gagtaaactt cacatcccga ccacatacag
     1681 gcaaagtaat ggcattaccg cgagccatta ctcctacgcg cgcaattaac gaatccacca
     1741 tcggggcagc tggtgtcgat aacgaagtat cttcaaccgg ttgagtattg agcgtatgtt
     1801 ttggaataac aggcgcacgc ttcattatct aatctcccag cgtggtttaa tcagacgatc
     1861 gaaaatttca ttgcagacag gttcccaaat agaaagagca tttctccagg caccagttga
     1921 agagcgttga tcaatggcct gttcaaaaac agttctcatc cggatctgac ctttaccaac
     1981 ttcatccgtt tcacgtacaa cattttttag aaccatgctt ccccaggcat cccgaatttg
     2041 ctcctccatc cacggggact gagagccatt actattgctg tatttggtaa gcaaaatacg
     2101 tacatcaggc tcgaaccctt taagatcaac gttcttgagc agatcacgaa gcatatcgaa
     2161 aaactgcagt gcggaggtgt agtcaaacaa ctcagcaggc gtgggaacaa tcagcacatc
     2221 agcagcacat acgacattaa tcgtgccgat acccaggtta ggcgcgctgt caataactat
     2281 gacatcatag tcatgagcaa cagtttcaat ggccagtcgg agcatcaggt gtggatcggt
     2341 gggcagttta ccttcatcaa atttgcccat taactcagtt tcaatacggt gcagagccag
     2401 acaggaagga ataatgtcaa gccccggcca gcaagtgggc tttattgcat aagtgacatc
     2461 gtccttttcc ccaagataga aaggcaggag agtgtcttct gcatgaatat gaagatctgg
     2521 tacccatccg tgatacattg aggctgttcc ctgggggtcg ttaccttcca cgagcaaaac
     2581 acgtagcccc ttcagagcca gatcctgagc aagatgaaca gaaactgagg ttttgtaaac
     2641 gccaccttta tgggcagcaa ccccgatcac cggtggaaat acgtcttcag cacgtcgcaa
     2701 tcgcgtacca aacacatcac gcatatgatt aatttgttca attgtataac caacacgttg
     2761 ctcaacccgt cctcgaattt ccatatccgg gtgcggtagt cgccctgctt tctcggcatc
     2821 tctgatagcc tgagaagaaa ccccaactaa atccgctgct tcacctattc tccagcgccg
     2881 ggttattttc ctcgcttccg ggctgtcatc attaaactgt gcaatggcga tagccttcgt
     2941 catttcatga ccagcgttta tgcactggtt aagtgtttcc atgagtttca ttctgaacat
     3001 cctttaatca ttgctttgcg tttttttatt aaatcttgca atttactgca aagcaacaac
     3061 aaaatcgcaa agtcatcaaa aaaccgcaaa gttgtttaaa ataagagcaa cactacaaaa
     3121 ggagataaga agagcacata cctcagtcac ttattatcac tagcgctcgc cgcagccgtg
     3181 taaccgagca tagcgagcga actggcgagg aagcaaagaa gaactgttct gtcagatagc
     3241 tcttacgctc agcgcaagaa gaaatatcca ccgtgggaaa aactccaggt agaggtacac
     3301 acgcggatag ccaattcaga gtaataaact gtgataatca accctcatca atgatgacga
     3361 actaaccccc gatatcaggt cacatgacga agggaaagag aaggaaatca actgtgacaa
     3421 actgccctca aatttggctt ccttaaaaat tacagttcaa aaagtatgag aaaatccatg
     3481 caggctgaag gaaacagcaa aactgtgaca aattaccctc agtaggtcag aacaaatgtg
     3541 acgaaccacc ctcaaatctg tgacagataa ccctcagact atcctgtcgt catggaagtg
     3601 atatcgcgga aggaaaatac gatatgagtc gtctggcggc ctttcttttt ctcaatgtat
     3661 gagaggcgca ttggagttct gctgttgatc tcattaacac agacctgcag gaagcggcgg
     3721 cggaagtcag gcatacgctg gtaactttga ggcagctggt aacgctctat gatccagtcg
     3781 attttcagag agacgatgcc tgagccatcc ggcttacgat actgacacag ggattcgtat
     3841 aaacgcatgg catacggatt ggtgatttct tttgtttcac taagccgaaa ctgcgtaaac
     3901 cggttctgta acccgataaa gaagggaatg agatatgggt tgatatgtac actgtaaagc
     3961 cctctggatg gactgtgcgc acgtttgata aaccaaggaa aagattcata gcctttttca
     4021 tcgccggcat cctcttcagg gcgataaaaa accacttcct tccccgcgaa actcttcaat
     4081 gcctgccgta tatccttact ggcttccgca gaggtcaatc cgaatatttc agcatattta
     4141 gcaacatgga tctcgcagat accgtcatgt tcctgtaggg tgccatcaga ttttctgatc
     4201 tggtcaacga acagatacag catacgtttt tgatcccggg agagactata tgccgcctca
     4261 gtgaggtcgt ttgactggac gattcgcggg ctatttttac gtttcttgtg attgataacc
     4321 gctgtttccg ccatgacaga tccatgtgaa gtgtgacaag tttttagatt gtcacactaa
     4381 ataaaaaaga gtcaataagc agggataact ttgtgaaaaa acagcttctt ctgagggcaa
     4441 tttgtcacag ggttaagggc aatttgtcac agacaggact gtcatttgag ggtgatttgt
     4501 cacactgaaa gggcaatttg tcacaacacc ttctctagaa ccagcatgga taaaggccta
     4561 caaggcgctc taaaaaagaa gatctaaaaa ctataaaaaa aataattata aaaatatccc
     4621 cgtggataag tggataaccc caagggaagt tttttcaggc atcgtgtgta agcagaatat
     4681 ataagtgctg ttccctggtg cttcctcgct cactcgagct acggggtctg acgctcagtg
     4741 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     4801 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     4861 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     4921 ttcatccata gttgcctgac tgcccgtcgt gtagataact acgatacggg agggcttacc
     4981 atctggcccc agtgctgcaa tgataccgcg ggacccacgc tcaccggctc cagatttatc
     5041 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     5101 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     5161 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     5221 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     5281 aaaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     5341 gttatcactc atgcttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     5401 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     5461 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     5521 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     5581 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     5641 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     5701 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     5761 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     5821 aataggggtt ccgcgcacat ttccccgaaa agtgccacct ggcggccgcc tttcagcaaa
     5881 aaaccccgcg agacccccga agaggccccg cggggttatg ctaggtcgac ggagctcgaa
     5941 ttctaatacg actcactata gggagacctt gacaattaat catcggctcg tataatgcca
     6001 gaagcttagc aaaagctaaa accaggagct atttaatggc aacagttaac cagctggtac
     6061 gcaaaccacg tgctcgcaaa gttgcgaaaa gcaacgtgcc tgcgctggaa gcatgcccgc
     6121 aaaaacgtgg cgtatgtact cgtgtatata ctaccactcc taaaaaaccg aactccgcgc
     6181 tgcgtaaagt atgccgtgtt cgtctgacta acggtttcga agtgacttcc tacatcggtg
     6241 gtgaaggtca caacctgcag gagcactccg tgatcctgat ccgtggcggt cgtgttaaag
     6301 acctcccggg tgttcgttac cacaccgtac gtggtgcgct tgactgctcc ggcgttaaag
     6361 accgtaagca ggctcgttcc aagtatggcg tgaagcgtcc taaggcttaa tggtttaatt
     6421 aacgcagcct gaatggcgaa tagaagttta aacgctagac ctagcataac cccgcggggc
     6481 ctcttcgggg gtctcgcggg gttttttgct gaaaggctag acctagcata accccgcggg
     6541 gcctcttcgg gggtctcgcg gggttttttg ctgaaaggct agacctagca taaccccgcg
     6601 gggcctcttc gggggtctcg cggggttttt tgctgaaagg ctagacctag cataaccccg
     6661 cggggcctct tcgggggtct cgcggggttt tttgctgaaa ggctagacct agcataaccc
     6721 cgcggggcct cttcgggggt ctcgcggggt tttttgctga aaggctagga agtttaaacg
     6781 ctagacctag cataaccccg cggggcctct tcgggggtct cgcggggttt tttgctgaaa
     6841 ggctagacct agcataaccc cgcggggcct cttcgggggt ctcgcggggt tttttgctga
     6901 aaggctagac ctagcataac cccgcggggc ctcttcgggg gtctcgcggg gttttttgct
     6961 gaaaggctag acctagcata accccgcggg gcctcttcgg gggtctcgcg gggttttttg
     7021 ctgaaaggct agacctagca taaccccgcg gggcctcttc gggggtctcg cggggttttt
     7081 tgctgaaagg ctaggatata ttgatccttg acagcttatc atcgataagc tttaatgcgg
     7141 tagtttatca cagttgctaa cgcagtcagg caccgtgtac gaatagttcg acaaagatcg
     7201 cattggtaat tacgttactc gatgccatgg ggattggcct tatcatgcca gtcttgccaa
     7261 cgttattacg tgaatttatt gcttcggaag atatcgctaa ccactttggc gtattgcttg
     7321 cactttatgc gttaatgcag gttatctttg ctccttggct tggaaaaatg tctgaccgat
     7381 ttggtcggcg cccagtgctg ttgttgtcat taataggcgc atcgctggat tacttattgc
     7441 tggctttttc aagtgcgctt tggatgctgt atttaggccg tttgctttca gggatcacag
     7501 gagctactgg ggctgtcgcg gcatcggtca ttgccgatac cacctcagct tctcaacgcg
     7561 tgaagtggtt cggttggtta ggggcaagtt ttgggcttgg tttaatagcg gggcctatta
     7621 ttggtggttt tgcaggagag atttcaccgc atagtccctt ttttatcgct gcgttgctaa
     7681 atattgtcac tttccttgtg gttatgtttt ggttccgtga aaccaaaaat acacgtgata
     7741 atacagatac cgaagtaggg gttgagacgc aatcgaattc ggtatacatc actttattta
     7801 aaacgatgcc cattttgttg attatttatt tttcagcgca attgataggc caaattcccg
     7861 caacggtgtg ggtgctattt accgaaaatc gttttggatg gaatagcatg atggttggct
     7921 tttcattagc gggtcttggt cttttacact cagtattcca agcctttgtg gcaggaagaa
     7981 tagccactaa atggggcgaa aaaacggcag tactgctcgg atttattgca gatagtagtg
     8041 catttgcctt tttagcgttt atatctgaag gttggttagt tttccctgtt ttaattttat
     8101 tggctggtgg tgggatcgct ttacctgcat tacagggagt gatgtctatc caaacaaaga
     8161 gtcatcagca aggtgcttta cagggattat tggtgagcct taccaatgca accggtgtta
     8221 ttggcccatt actgtttgct gttatttata atcattcact accaatttgg gatggctgga
     8281 tttggattat tggtttagcg ttttactgta ttattatcct gctatcgatg accttcatgt
     8341 taacccctca agctcagggg agtaaacagg agacaagtgc ttagtgatcc aattcttgaa
     8401 g
//
