LOCUS       pEMF036                 5015 bp ds-DNA     circular     22-APR-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    3334..3372
                     /label="Linker"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3099..3172
                     /label="PLtetO-1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1430..1430
                     /label="+1 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     rep_origin      complement(join(4715..5015,1..382))
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    1903..2031
                     /label="SYNZIP 17"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    3373..3678
                     /label="sMHT 130-230"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3153..3153
                     /label="+1 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4118..4140
                     /label="Sequencing primer fw SR359"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3782..3816
                     /label="Constutive promoter J23106"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(1218..1218)
                     /label="+1 site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(1207..1212)
                     /label="-10 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(3016..3092)
                     /label="PdcuS"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1376..1449
                     /label="PLtetO-1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1450..1476
                     /label="sRBS (9923)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     terminator      387..492
                     /label="T0"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     misc_feature    1297..1329
                     /label="Cloning primer fw SR224"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(2278..2997)
                     /label="sfgfp"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2044..2172
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    3173..3207
                     /label="sRBS (10002 au)"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1126..1150
                     /label="Primer sequencing SR53"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(1200..1200)
                     /label="+1 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1418..1423
                     /label="-10 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1815..1816
                     /label="split site"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    3141..3146
                     /label="-10 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1864..1902
                     /label="Linker"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1477..1863
                     /label="sMHT 1 - 129"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1395..1400
                     /label="-35 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(2998..3015)
                     /label="sRBS (4,296)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(1248..1253)
                     /label="-35 region"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3679..3684
                     /label="sMHT 114 - 230"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    complement(2911..2932)
                     /label="Sequencing Primer rev SR143"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(2278..3092)
                     /label="(Extraneous DNA from previous construct)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    4614..4635
                     /label="Primer sequencing SR29"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(1225..1230)
                     /label="-10 region"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2173..2277
                     /label="pEXT20 expression vector source"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3817..3850
                     /label="sRBS (50,000)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(1231..1236)
                     /label="-35 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     terminator      4478..4582
                     /label="T1"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     misc_feature    3920..3943
                     /label="Sequencing primer rev SR360"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             509..1168
                     /label="CmR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             3851..4477
                     /label="tetR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    3208..3333
                     /label="SYNZIP 18"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3118..3123
                     /label="-35 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     terminator      3685..3736
                     /label="L3S1P52"
                     /ApEinfo_revcolor=#f6989d
                     /ApEinfo_fwdcolor=#f6989d
     primer          complement(1456..1476)
                     /label="oEMF341"
                     /note="sequence: TTTTTTTGGTCTCTCATCAAAACTTCCTTTGTATGGGT"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          complement(1837..1861)
                     /label="oEMF338"
                     /note="sequence: AAAAAAAGGTCTCTGCCAGGTAAACACATCTTCTTTCAC"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          1475..1494
                     /label="oEMF331"
                     /note="sequence: TTTTTTTGGTCTCTGATGAGCACCGTGGCGAAC"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(3355..3372)
                     /label="oEMF342"
                     /note="sequence: TTTTTTTggtctctCGGtGCGGCCGCGGACCCACC"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(3655..3677)
                     /label="oEMF340"
                     /note="sequence: TTTTTTTGGTCTCATAGTTGTTCATTTTTTTCCAGCG"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          complement(3654..3677)
                     /label="oEMF336"
                     /note="sequence: TTTTTTTGGTCTCGTAGTTGTTCATTTTTTTCCAGCGC"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          1864..1881
                     /label="oEMF339"
                     /note="sequence: TTTTTTTGGTCTCTTGGCGCGCGGCCGCGGGTGGCGGT"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          3373..3390
                     /label="oEMF343"
                     /note="sequence: TTTTTTTGGTCTCCaCCGGAACAGCCGTTTGAT"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          1864..1881
                     /label="oEMF333"
                     /note="sequence: TTTTTTTGGTCTCATGCCGGCGGCCGCGGGTGGCGGT"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          3674..3703
                     /label="oEMF337"
                     /note="sequence: GACACAGGGTCTCCACTAAtaataaTCTAACTAAAAAGGCCTCC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          1678..1695
                     /label="oEMF335"
                     /note="sequence: TTTTTTTGGTCTCGCaAACGGCAAAGGCCTGGTG"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
ORIGIN
        1 TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA
       61 CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG
      121 TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT
      181 CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC
      241 ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA
      301 TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA
      361 CGTTAAGGGA TTTTGGTCAT GACTAGTGCT TGGATTCTCA CCAATAAAAA ACGCCCGGCG
      421 GCAACCGAGC GTTCTGAACA AATCCAGATG GAGTTCTGAG GTCATTACTG GATCTATCAA
      481 CAGGAGTCCA AGCGAGCTCG ATATCAAATT ACGCCCCGCC CTGCCACTCA TCGCAGTACT
      541 GTTGTAATTC ATTAAGCATT CTGCCGACAT GGAAGCCATC ACAGACGGCA TGATGAACCT
      601 GAATCGCCAG CGGCATCAGC ACCTTGTCGC CTTGCGTATA ATATTTGCCC ATGGTGAAAA
      661 CGGGGGCGAA GAAGTTGTCC ATATTGGCCA CGTTTAAATC AAAACTGGTG AAACTCACCC
      721 AGGGATTGGC TGAGACGAAA AACATATTCT CAATAAACCC TTTAGGGAAA TAGGCCAGGT
      781 TTTCACCGTA ACACGCCACA TCTTGCGAAT ATATGTGTAG AAACTGCCGG AAATCGTCGT
      841 GGTATTCACT CCAGAGCGAT GAAAACGTTT CAGTTTGCTC ATGGAAAACG GTGTAACAAG
      901 GGTGAACACT ATCCCATATC ACCAGCTCAC CGTCTTTCAT TGCCATACGA AATTCCGGAT
      961 GAGCATTCAT CAGGCGGGCA AGAATGTGAA TAAAGGCCGG ATAAAACTTG TGCTTATTTT
     1021 TCTTTACGGT CTTTAAAAAG GCCGTAATAT CCAGCTGAAC GGTCTGGTTA TAGGTACATT
     1081 GAGCAACTGA CTGAAATGCC TCAAAATGTT CTTTACGATG CCATTGGGAT ATATCAACGG
     1141 TGGTATATCC AGTGATTTTT TTCTCCATTT TAGCTTCCTT AGCTCCTGAA AATCTCGATA
     1201 ACTCAAAAAA TACGCCCGGT AGTGATCTTA TTTCATTATG GTGAAAGTTG GAACCTCTTA
     1261 CGTGCCGATC AACGTCTCAT TTTCGCCAGA TATCGACGTC TAAGAAACCA TTATTATCAT
     1321 GACATTAACC TATAAAAATA GGCGTATCAC GAGGCCCTTT CGTCTTCACC TCGAGTCCCT
     1381 ATCAGTGATA GAGATTGACA TCCCTATCAG TGATAGAGAT ACTGAGCACA TCAGCAGGAC
     1441 GCACTGACCG ATAGGACCCA TACAAAGGAA GTTTTGATGA GCACCGTGGC GAACATTGCG
     1501 CCGGTGTTTA CCGGCGATTG CAAAACCATT CCGACCCCGG AAGAATGCGC GACCTTTCTG
     1561 TATAAAGTGG TGAACAGCGG CGGCTGGGAA AAATGCTGGG TGGAAGAAGT GATTCCGTGG
     1621 GATCTGGGCG TGCCGACCCC GCTGGTGCTG CATCTGGTGA AAAACAACGC GCTGCCGAAC
     1681 GGCAAAGGCC TGGTGCCGGG CTGCGGCGGC GGCTATGATG TGGTGGCGAT GGCGAACCCG
     1741 GAACGCTTTA TGGTGGGCCT GGATATTAGC GAAAACGCGC TGAAAAAAGC GCGCGAAACC
     1801 TTTAGCACCA TGCCGAACAG CAGCTGCTTT AGCTTTGTGA AAGAAGATGT GTTTACCTGG
     1861 CGCGCGGCCG CGGGTGGCGG TGGCTCTGGA GGTGGTGGGT CCATGAACGA AAAAGAAGAA
     1921 CTGAAATCTA AAAAAGCGGA ACTGCGCAAC CGCATCGAAC AGCTGAAACA GAAACGCGAA
     1981 CAGCTGAAAC AGAAAATCGC GAACCTGCGC AAAGAAATCG AAGCGTATAA ATAATAATCT
     2041 AGACCAGGCA TCAAATAAAA CGAAAGGCTC AGTCGAAAGA CTGGGCCTTT CGTTTTATCT
     2101 GTTGTTTGTC GGTGAACGCT CTCTACTAGA GTCACACTGG CTCACCTTCG GGTGGGCCTT
     2161 TCTGCGTTTA TAGTAAATCA CTGCATAATT CGTGTCGCTC AAGGCGCACT CCCGTTCTGG
     2221 ATAATGTTTT TTGCGCCGAC ATCATAACGG TTCTGGCAAA TATTCTGAAA TGAGCTGTCA
     2281 TCATTTGTAC AGTTCATCCA TACCATGCGT GATGCCCGCT GCGGTTACGA ACTCCAGCAG
     2341 AACCATATGA TCGCGTTTCT CGTTCGGATC TTTAGACAGA ACGCTTTGCG TGCTCAGATA
     2401 GTGATTGTCT GGCAGCAGAA CAGGACCATC ACCGATTGGA GTGTTTTGCT GGTAGTGATC
     2461 AGCCAGCTGC ACGCTGCCAT CCTCCACGTT GTGGCGAATT TTAAAATTCG CTTTAATGCC
     2521 ATTTTTTTGT TTATCGGCGG TGATGTAAAC ATTGTGGCTG TTAAAATTGT ATTCCAGCTT
     2581 ATGGCCCAGG ATATTGCCGT CTTCTTTAAA GTCAATGCCT TTCAGCTCAA TGCGGTTTAC
     2641 CAGGGTATCG CCTTCAAATT TCACTTCCGC ACGCGTTTTG TACGTGCCGT CATCCTTAAA
     2701 GGAAATCGTG CGTTCCTGCA CATAGCCTTC CGGCATGGCG GACTTGAAGA AGTCATGCTG
     2761 CTTCATATGG TCCGGATAAC GAGCAAAGCA CTGAACACCA TAAGTCAGCG TCGTTACCAG
     2821 AGTCGGCCAA GGTACCGGCA GTTTACCAGT AGTACAGATG AACTTCAGCG TCAGTTTACC
     2881 ATTAGTTGCG TCACCTTCAC CCTCGCCACG CACGGAAAAC TTATGACCGT TGACATCACC
     2941 ATCCAGTTCC ACCAGAATAG GGACGACACC AGTGAACAGC TCTTCGCCTT TACGCATCTA
     3001 GTATTTCTCC TCTTTTTCTT AAGCATTATC TCTGATGAGG CGGGTAATTC AAAGGGAGTA
     3061 AGAATGATTG GCTATATAGG GGAAGAGACT CTACCGGTTC CCTATCAGTG ATAGAGATTG
     3121 ACATCCCTAT CAGTGATAGA GATACTGAGC ACATCAGCAG GACGCACTGA CCAACTAACG
     3181 AAAACGTCAC ACGGAGCAGG TTATTAAATG TCTATCGCGG CCACCCTGGA AAACGACTTA
     3241 GCACGCCTTG AGAATGAAAA TGCTCGTCTG GAGAAAGATA TCGCGAATCT CGAGCGTGAC
     3301 CTGGCAAAAT TAGAGCGTGA GGAAGCTTAT TTCGGTGGCG GTGGCTCTGG AGGTGGTGGG
     3361 TCCGCGGCCG CaCCGGAACA GCCGTTTGAT TTTATTTTTG ATTATGTGTT TTTTTGCGCG
     3421 ATTGATCCGA AAATGCGCCC GGCGTGGGGC AAAGCGATGT ATGAACTGCT GAAACCGGAT
     3481 GGCGAACTGA TTACCCTGAT GTATCCGATT ACCAACCATG AAGGCGGCCC GCCGTTTAGC
     3541 GTGAGCGAAA GCGAATATGA AAAAGTGCTG GTGCCGCTGG GCTTTAAACA GCTGAGCCTG
     3601 GAAGATTATA GCGATCTGGC GGTGGAACCG CGCAAAGGCA AAGAAAAACT GGCGCGCTGG
     3661 AAAAAAATGA ACAACTAAta ataaTCTAAC TAAAAAGGCC TCCCAAATCG GGGGGCCTTT
     3721 TTTATTGATA ACAAAAGGTA CCCGTGGATC CTCTAGAGTC GACCTGCAGG CATGCAAGCT
     3781 TTTTACGGCT AGCTCAGTCC TAGGTATAGT GCTAGCCCAG CCAGAGAAAC ACTCTTTAAC
     3841 AGGGGGTAGT ATGATGTCTC GTTTAGATAA AAGTAAAGTG ATTAACAGCG CATTAGAGCT
     3901 GCTTAATGAG GTCGGAATCG AAGGTTTAAC AACCCGTAAA CTCGCCCAGA AGCTAGGTGT
     3961 AGAGCAGCCT ACATTGTATT GGCATGTAAA AAATAAGCGG GCTTTGCTCG ACGCCTTAGC
     4021 CATTGAGATG TTAGATAGGC ACCATACTCA CTTTTGCCCT TTAGAAGGGG AAAGCTGGCA
     4081 AGATTTTTTA CGTAATAACG CTAAAAGTTT TAGATGTGCT TTACTAAGTC ATCGCGATGG
     4141 AGCAAAAGTA CATTTAGGTA CACGGCCTAC AGAAAAACAG TATGAAACTC TCGAAAATCA
     4201 ATTAGCCTTT TTATGCCAAC AAGGTTTTTC ACTAGAGAAT GCATTATATG CACTCAGCGC
     4261 AGTGGGGCAT TTTACTTTAG GTTGCGTATT GGAAGATCAA GAGCATCAAG TCGCTAAAGA
     4321 AGAAAGGGAA ACACCTACTA CTGATAGTAT GCCGCCATTA TTACGACAAG CTATCGAATT
     4381 ATTTGATCAC CAAGGTGCAG AGCCAGCCTT CTTATTCGGC CTTGAATTGA TCATATGCGG
     4441 ATTAGAAAAA CAACTTAAAT GTGAAAGTGG GTCTTAAGGC ATCAAATAAA ACGAAAGGCT
     4501 CAGTCGAAAG ACTGGGCCTT TCGTTTTATC TGTTGTTTGT CGGTGAACGC TCTCCTGAGT
     4561 AGGACAAATC CGCCGCCCTA GACCTAGGCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT
     4621 CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG
     4681 CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA
     4741 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
     4801 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
     4861 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
     4921 TTTCTCAATG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
     4981 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGC
//