LOCUS       pEMF044                 3337 bp ds-DNA     circular     06-DEC-2020
DEFINITION  .
KEYWORDS    "Author" "Plasmid" "Freezer" "Rack" "Box"                          
FEATURES             Location/Qualifiers
     misc_feature    2310..2332
                     /label="Sequencing primer fw SR359"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     terminator      1877..1928
                     /label="L3S1P52"
                     /ApEinfo_revcolor=#f6989d
                     /ApEinfo_fwdcolor=#f6989d
     terminator      257..362
                     /label="T0"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     misc_feature    2009..2042
                     /label="sRBS (50,000)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     terminator      2670..2774
                     /label="T1"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     CDS             2043..2669
                     /label="tetR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2112..2135
                     /label="Sequencing primer rev SR360"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(1088..1088)
                     /label="+1 site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1520..1870
                     /label="sMHT 114 - 230"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    complement(1101..1106)
                     /label="-35 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1265..1270
                     /label="-35 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1974..2008
                     /label="Constutive promoter J23106"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1300..1300
                     /label="+1 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(1070..1070)
                     /label="+1 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1355..1480
                     /label="SYNZIP 18"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             379..1038
                     /label="CmR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(1095..1100)
                     /label="-10 region"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1246..1319
                     /label="PLtetO-1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(1118..1123)
                     /label="-35 region"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2806..2827
                     /label="Primer sequencing SR29"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    996..1020
                     /label="Primer sequencing SR53"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1320..1354
                     /label="sRBS (10002 au)"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1167..1199
                     /label="Cloning primer fw SR224"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1481..1519
                     /label="Linker"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1871..1876
                     /label="sMHT 114 - 230"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     rep_origin      complement(join(2907..3337,1..252))
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    complement(1077..1082)
                     /label="-10 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1288..1293
                     /label="-10 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          1154..1174
                     /label="oEMF061"
                     /note="sequence: CGCCAGATATCGACGTCTAAG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(1228..1245)
                     /label="oEMF387"
                     /note="sequence: AAAAAAAGGTCTCCGACTCGAGGTGAAGACGAAA"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          1246..1267
                     /label="oEMF386"
                     /note="sequence: AAAAAAAGGTCTCCAGTCCCTATCAGTGATAGAGATTG"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC
       61 CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA
      121 GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG
      181 ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA
      241 TTTTGGTCAT GACTAGTGCT TGGATTCTCA CCAATAAAAA ACGCCCGGCG GCAACCGAGC
      301 GTTCTGAACA AATCCAGATG GAGTTCTGAG GTCATTACTG GATCTATCAA CAGGAGTCCA
      361 AGCGAGCTCG ATATCAAATT ACGCCCCGCC CTGCCACTCA TCGCAGTACT GTTGTAATTC
      421 ATTAAGCATT CTGCCGACAT GGAAGCCATC ACAGACGGCA TGATGAACCT GAATCGCCAG
      481 CGGCATCAGC ACCTTGTCGC CTTGCGTATA ATATTTGCCC ATGGTGAAAA CGGGGGCGAA
      541 GAAGTTGTCC ATATTGGCCA CGTTTAAATC AAAACTGGTG AAACTCACCC AGGGATTGGC
      601 TGAGACGAAA AACATATTCT CAATAAACCC TTTAGGGAAA TAGGCCAGGT TTTCACCGTA
      661 ACACGCCACA TCTTGCGAAT ATATGTGTAG AAACTGCCGG AAATCGTCGT GGTATTCACT
      721 CCAGAGCGAT GAAAACGTTT CAGTTTGCTC ATGGAAAACG GTGTAACAAG GGTGAACACT
      781 ATCCCATATC ACCAGCTCAC CGTCTTTCAT TGCCATACGA AATTCCGGAT GAGCATTCAT
      841 CAGGCGGGCA AGAATGTGAA TAAAGGCCGG ATAAAACTTG TGCTTATTTT TCTTTACGGT
      901 CTTTAAAAAG GCCGTAATAT CCAGCTGAAC GGTCTGGTTA TAGGTACATT GAGCAACTGA
      961 CTGAAATGCC TCAAAATGTT CTTTACGATG CCATTGGGAT ATATCAACGG TGGTATATCC
     1021 AGTGATTTTT TTCTCCATTT TAGCTTCCTT AGCTCCTGAA AATCTCGATA ACTCAAAAAA
     1081 TACGCCCGGT AGTGATCTTA TTTCATTATG GTGAAAGTTG GAACCTCTTA CGTGCCGATC
     1141 AACGTCTCAT TTTCGCCAGA TATCGACGTC TAAGAAACCA TTATTATCAT GACATTAACC
     1201 TATAAAAATA GGCGTATCAC GAGGCCCTTT CGTCTTCACC TCGAGTCCCT ATCAGTGATA
     1261 GAGATTGACA TCCCTATCAG TGATAGAGAT ACTGAGCACA TCAGCAGGAC GCACTGACCA
     1321 ACTAACGAAA ACGTCACACG GAGCAGGTTA TTAAATGTCT ATCGCGGCCA CCCTGGAAAA
     1381 CGACTTAGCA CGCCTTGAGA ATGAAAATGC TCGTCTGGAG AAAGATATCG CGAATCTCGA
     1441 GCGTGACCTG GCAAAATTAG AGCGTGAGGA AGCTTATTTC GGTGGCGGTG GCTCTGGAGG
     1501 TGGTGGGTCC GCGGCCGCaA ACAGCAGCTG CTTTAGCTTT GTGAAAGAAG ATGTGTTTAC
     1561 CTGGCGCCCG GAACAGCCGT TTGATTTTAT TTTTGATTAT GTGTTTTTTT GCGCGATTGA
     1621 TCCGAAAATG CGCCCGGCGT GGGGCAAAGC GATGTATGAA CTGCTGAAAC CGGATGGCGA
     1681 ACTGATTACC CTGATGTATC CGATTACCAA CCATGAAGGC GGCCCGCCGT TTAGCGTGAG
     1741 CGAAAGCGAA TATGAAAAAG TGCTGGTGCC GCTGGGCTTT AAACAGCTGA GCCTGGAAGA
     1801 TTATAGCGAT CTGGCGGTGG AACCGCGCAA AGGCAAAGAA AAACTGGCGC GCTGGAAAAA
     1861 AATGAACAAC taataaTCTA ACTAAAAAGG CCTCCCAAAT CGGGGGGCCT TTTTTATTGA
     1921 TAACAAAAGG TACCCGTGGA TCCTCTAGAG TCGACCTGCA GGCATGCAAG CTTTTTACGG
     1981 CTAGCTCAGT CCTAGGTATA GTGCTAGCCC AGCCAGAGAA ACACTCTTTA ACAGGGGGTA
     2041 GTATGATGTC TCGTTTAGAT AAAAGTAAAG TGATTAACAG CGCATTAGAG CTGCTTAATG
     2101 AGGTCGGAAT CGAAGGTTTA ACAACCCGTA AACTCGCCCA GAAGCTAGGT GTAGAGCAGC
     2161 CTACATTGTA TTGGCATGTA AAAAATAAGC GGGCTTTGCT CGACGCCTTA GCCATTGAGA
     2221 TGTTAGATAG GCACCATACT CACTTTTGCC CTTTAGAAGG GGAAAGCTGG CAAGATTTTT
     2281 TACGTAATAA CGCTAAAAGT TTTAGATGTG CTTTACTAAG TCATCGCGAT GGAGCAAAAG
     2341 TACATTTAGG TACACGGCCT ACAGAAAAAC AGTATGAAAC TCTCGAAAAT CAATTAGCCT
     2401 TTTTATGCCA ACAAGGTTTT TCACTAGAGA ATGCATTATA TGCACTCAGC GCAGTGGGGC
     2461 ATTTTACTTT AGGTTGCGTA TTGGAAGATC AAGAGCATCA AGTCGCTAAA GAAGAAAGGG
     2521 AAACACCTAC TACTGATAGT ATGCCGCCAT TATTACGACA AGCTATCGAA TTATTTGATC
     2581 ACCAAGGTGC AGAGCCAGCC TTCTTATTCG GCCTTGAATT GATCATATGC GGATTAGAAA
     2641 AACAACTTAA ATGTGAAAGT GGGTCTTAAG GCATCAAATA AAACGAAAGG CTCAGTCGAA
     2701 AGACTGGGCC TTTCGTTTTA TCTGTTGTTT GTCGGTGAAC GCTCTCCTGA GTAGGACAAA
     2761 TCCGCCGCCC TAGACCTAGG CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG
     2821 GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC
     2881 CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC
     2941 CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA
     3001 CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC
     3061 CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAA
     3121 TGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG
     3181 CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC
     3241 AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA
     3301 GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGT
//