LOCUS       pDH012                  4137 bp ds-DNA     circular     18-NOV-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     RBS             3309..3393
                     /label="BCD2"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2055..2071
                     /label="tetRo"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        2049..2084
                     /label="TetRo_apFAB154"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2049..2054
                     /label="-35 box"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3154..3176
                     /label="rpoC terminator (min, modified)"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    3910..3936
                     /label="HA Tag, Stopstop"
                     /ApEinfo_revcolor=#7ea7d8
                     /ApEinfo_fwdcolor=#7ea7d8
     misc_feature    3122..3142
                     /label="rpoC spacer"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    3279..3295
                     /label="tetRo"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3379..3387
                     /label="variable"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    3296..3301
                     /label="-10 box"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    4009..4137
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     CDS             2182..2199
                     /label="6xHis"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3199..3212
                     /label="Point mutation present! Check Description!"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2072..2077
                     /label="-10 box"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2650..2778
                     /label="SYNZIP 17"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    3520..3558
                     /label="Linker"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    155..946
                     /label="SpecR"
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_fwdcolor=#ff00ff
     CDS             2230..2261
                     /label="T7 tag"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3096..3272
                     /label="3FStops-rpoCspacer-rpoC terminator-T7A1pause-SSpB"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     terminator      2872..2899
                     /label="rrnB_T2_terminator"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2272..2610
                     /label="sMHT_113_F1"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        3273..3308
                     /label="TetRo_apFAB154"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3394..3519
                     /label="SYNZIP 18"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2170..2261
                     /label="pET28b"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    3211..3272
                     /label="Insulator"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2170..2172
                     /label="ATG"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             2209..2226
                     /label="thrombin site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3559..3909
                     /label="sMHT_113_F2"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2262..2271
                     /label="linker"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    3273..3278
                     /label="-35 box"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2155..2163
                     /label="variable"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     RBS             2085..2169
                     /label="BCD2"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2611..2649
                     /label="Linker"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     promoter        7..154
                     /label="specR_promoter_from_pKD13"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     rep_origin      complement(1174..1861)
                     /label="p15A"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     terminator      complement(954..1059)
                     /label="T0"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
ORIGIN
        1 GGTACCAGCA AGCGAACCGG AATTGCCAGC TGGGGCGCCC TCTGGTAAGG TTGGGAAGCC
       61 CTGCAAAGTA AACTGGATGG CTTTCTTGCC GCCAAGGATC TGATGGCGCA GGGGATCAAG
      121 ATCTGATCAA GAGACAGGAT GAGGATCGTT TCGCATGAGG GAAGCGGTGA TCGCCGAAGT
      181 ATCGACTCAA CTATCAGAGG TAGTTGGCGT CATCGAGCGC CATCTCGAAC CGACGTTGCT
      241 GGCCGTACAT TTGTACGGCT CCGCAGTGGA TGGCGGCCTG AAGCCACACA GTGATATTGA
      301 TTTGCTGGTT ACGGTGACCG TAAGGCTTGA TGAAACAACG CGGCGAGCTT TGATCAACGA
      361 CCTTTTGGAA ACTTCGGCTT CCCCTGGAGA GAGCGAGATT CTCCGCGCTG TAGAAGTCAC
      421 CATTGTTGTG CACGACGACA TCATTCCGTG GCGTTATCCA GCTAAGCGCG AACTGCAATT
      481 TGGAGAATGG CAGCGCAATG ACATTCTTGC AGGTATCTTC GAGCCAGCCA CGATCGACAT
      541 TGATCTGGCT ATCTTGCTGA CAAAAGCAAG AGAACATAGC GTTGCCTTGG TAGGTCCAGC
      601 GGCGGAGGAA CTCTTTGATC CGGTTCCTGA ACAGGATCTA TTTGAGGCGC TAAATGAAAC
      661 CTTAACGCTA TGGAACTCGC CGCCCGACTG GGCTGGCGAT GAGCGAAATG TAGTGCTTAC
      721 GTTGTCCCGC ATTTGGTACA GCGCAGTAAC CGGCAAAATC GCGCCGAAGG ATGTCGCTGC
      781 CGACTGGGCA ATGGAGCGCC TGCCGGCCCA GTATCAGCCC GTCATACTTG AAGCTAGACA
      841 GGCTTATCTT GGACAAGAAG AAGATCGCTT GGCCTCGCGC GCAGATCAGT TGGAAGAATT
      901 TGTCCACTAC GTGAAAGGCG AGATCACCAA GGTAGTCGGC AAATAAGAGC TCGCTTGGAC
      961 TCCTGTTGAT AGATCCAGTA ATGACCTCAG AACTCCATCT GGATTTGTTC AGAACGCTCG
     1021 GTTGCCGCCG GGCGTTTTTT ATTGGTGAGA ATCCAAGCAC TAGTAACAAC TTATATCGTA
     1081 TGGGGCTGAC TTCAGGTGCT ACATTTGAAG AGATAAATTG CACTGAAATC TAGTAATATT
     1141 TTATCTGATT AATAAGATGA TCTTCTTGAG ATCGTTTTGG TCTGCGCGTA ATCTCTTGCT
     1201 CTGAAAACGA AAAAACCGCC TTGCAGGGCG GTTTTTCGAA GGTTCTCTGA GCTACCAACT
     1261 CTTTGAACCG AGGTAACTGG CTTGGAGGAG CGCAGTCACC AAAACTTGTC CTTTCAGTTT
     1321 AGCCTTAACC GGCGCATGAC TTCAAGACTA ACTCCTCTAA ATCAATTACC AGTGGCTGCT
     1381 GCCAGTGGTG CTTTTGCATG TCTTTCCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG
     1441 GCGCAGCGGT CGGACTGAAC GGGGGGTTCG TGCATACAGT CCAGCTTGGA GCGAACTGCC
     1501 TACCCGGAAC TGAGTGTCAG GCGTGGAATG AGACAAACGC GGCCATAACA GCGGAATGAC
     1561 ACCGGTAAAC CGAAAGGCAG GAACAGGAGA GCGCACGAGG GAGCCGCCAG GGGGAAACGC
     1621 CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCACTGA TTTGAGCGTC AGATTTCGTG
     1681 ATGCTTGTCA GGGGGGCGGA GCCTATGGAA AAACGGCTTT GCCGCGGCCC TCTCACTTCC
     1741 CTGTTAAGTA TCTTCCTGGC ATCTTCCAGG AAATCTCCGC CCCGTTCGTA AGCCATTTCC
     1801 GCTCGCCGCA GTCGAACGAC CGAGCGTAGC GAGTCAGTGA GCGAGGAAGC GGAATATATC
     1861 CCCTAGGTGG AGTCTGGCCT CAAATACAAA GTCTGCAAAG TCTCGAAATA TTAAACCAGA
     1921 GAATAAATAA TTAAACGCCA AAATATTTCT AGCAAAAACC CCAGTTATTA AACCGCCTAA
     1981 GTCCCCCAGG AAAGGGGGAT ATAACAGTAT AGATTTTGTC AGCCTTCAGC TTGGCTTTAC
     2041 CGTCAGAGTT GACATCCCTA TCAGTGATAG ATATAATGTG TGGAGGGCCC AAGTTCACTT
     2101 AAAAAGGAGA TCAACAATGA AAGCAATTTT CGTACTGAAA CATCTTAATC ATGCTAAGGA
     2161 GGTTTTCTAA TGGGCAGCAG CCATCATCAT CATCATCACA GCAGCGGCCT GGTGCCGCGC
     2221 GGCAGCCATA TGGCTAGCAT GACTGGTGGA CAGCAAATGG GTgCGGGATC CATGAGCACC
     2281 GTGGCGAACA TTGCGCCGGT GTTTACCGGC GATTGCAAAA CCATTCCGAC CCCGGAAGAA
     2341 TGCGCGACCT TTCTGTATAA AGTGGTGAAC AGCGGCGGCT GGGAAAAATG CTGGGTGGAA
     2401 GAAGTGATTC CGTGGGATCT GGGCGTGCCG ACCCCGCTGG TGCTGCATCT GGTGAAAAAC
     2461 AACGCGCTGC CGAACGGCAA AGGCCTGGTG CCGGGCTGCG GCGGCGGCTA TGATGTGGTG
     2521 GCGATGGCGA ACCCGGAACG CTTTATGGTG GGCCTGGATA TTAGCGAAAA CGCGCTGAAA
     2581 AAAGCGCGCG AAACCTTTAG CACCATGCCG GCGGCCGCGG GTGGCGGTGG CTCTGGAGGT
     2641 GGTGGGTCCA TGAACGAAAA AGAAGAACTG AAATCTAAAA AAGCGGAACT GCGCAACCGC
     2701 ATCGAACAGC TGAAACAGAA ACGCGAACAG CTGAAACAGA AAATCGCGAA CCTGCGCAAA
     2761 GAAATCGAAG CGTATAAAta atgaGGATCC TGATTTACGG CTAGCTCAGT CCTAGGTATA
     2821 GTGCTAGCAG GAGGTGTAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT Aagaaggcca
     2881 tcctgacgga tggccttttt gcgtttcgaa caattgaaaa aacctcgcgc cttacctgtt
     2941 gagtaatagt caaaagcctc cggtcggagg cttttgactt tctgcttact gaatttcggt
     3001 ggtgccgtta attaaccggt gggccctcat gataataatg gtttcttaga cgtccgaagt
     3061 tcctattctc tagaaagtat aggaacttcc ctaggGAATT CAAAAGATCT TAAGTAAGTA
     3121 AGAGTATACG TATATCGGCT AAtaacgtaT TaAGGCGCTT CGGCGCCttt ttttatgGGG
     3181 GTATTTTCAT CCCAATCCAC ACGTCCAACG CACAGCAAAC ACCACGTCGA CCCTATCAGC
     3241 TGCGTGCTTT CTATGAGTCG TTGCTGCAta acTTGACATC CCTATCAGTG ATAGATATAA
     3301 TGTGTGGAGG GCCCAAGTTC ACTTAAAAAG GAGATCAACA ATGAAAGCAA TTTTCGTACT
     3361 GAAACATCTT AATCATGCTA AGGAGGTTTT CTAATGTCTA TCGCGGCCAC CCTGGAAAAC
     3421 GACTTAGCAC GCCTTGAGAA TGAAAATGCT CGTCTGGAGA AAGATATCGC GAATCTCGAG
     3481 CGTGACCTGG CAAAATTAGA GCGTGAGGAA GCTTATTTCG GTGGCGGTGG CTCTGGAGGT
     3541 GGTGGGTCCG CGGCCGCaAA CAGCAGCTGC TTTAGCTTTG TGAAAGAAGA TGTGTTTACC
     3601 TGGCGCCCGG AACAGCCGTT TGATTTTATT TTTGATTATG TGTTTTTTTG CGCGATTGAT
     3661 CCGAAAATGC GCCCGGCGTG GGGCAAAGCG ATGTATGAAC TGCTGAAACC GGATGGCGAA
     3721 CTGATTACCC TGATGTATCC GATTACCAAC CATGAAGGCG GCCCGCCGTT TAGCGTGAGC
     3781 GAAAGCGAAT ATGAAAAAGT GCTGGTGCCG CTGGGCTTTA AACAGCTGAG CCTGGAAGAT
     3841 TATAGCGATC TGGCGGTGGA ACCGCGCAAA GGCAAAGAAA AACTGGCGCG CTGGAAAAAA
     3901 ATGAACAACT ACCCTTACGA TGTGCCTGAC TACGCGtaat gaCTGAGATC CGGCTGCTAA
     3961 CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATACC AGGCATCAAA
     4021 TAAAACGAAA GGCTCAGTCG AAAGACTGGG CCTTTCGTTT TATCTGTTGT TTGTCGGTGA
     4081 ACGCTCTCTA CTAGAGTCAC ACTGGCTCAC CTTCGGGTGG GCCTTTCTGC GTTTATA
//