LOCUS (corrected)EtoH04.(thre 4952 bp ds-DNA circular 30-JUN-2020 DEFINITION . FEATURES Location/Qualifiers terminator 3710..3837 /label="T7\terminator" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 1065..1074 /label="cspa trans start" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature 2635..2653 /label="Primering region" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 misc_feature 1193..1235 /label="RBS1" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 misc_feature 2610..2644 /label="RBS2stop" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 misc_feature 993..1192 /label="PgyrA" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 misc_feature 3686..3706 /label="N31" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 misc_feature 4044..4904 /label="AMP" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac misc_feature 2629..2634 /label="RBS" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 CDS 130..718 /label="pMB1" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 gene 1236..2609 /label="ADA codon optimization" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 CDS 2645..3685 /label="ADH2 CDS" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac misc_feature 3907..3927 /label="N36" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac misc_feature 59..77 /label="N22" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 misc_feature 1176..1182 /label="Ppnp trans start" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 misc_feature 2611..2628 /label="Muta SE" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature 966..992 /label="threeUTR" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 ORIGIN 1 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtc 61 acacaacata gccacggagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa 121 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 181 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 241 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg 301 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 361 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 421 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 481 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct 541 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 601 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 661 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 721 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt 781 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 841 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 901 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatgctg 961 gatccTaaca tcatcatcac catcactaaa aGgaggcgac ggcttcaaat ttagcgatct 1021 cttcgtggtc tacgttatgg ttttccggcg atttttcggc attcattggc acttctactc 1081 cgtaattggc aagacaaacg agtatatcag gcattggatg tgaataaagc gtataggttt 1141 acctcaaact gcgcggctgt gttataattt gcgacctttg aatccgggat actagaaata 1201 attttgttta actttaagaa ggagatatac atatggaaca ctccgtgatc gaaccgactg 1261 taccgatgcc catgccagcg atgtttgacg caccgagcgg cattttcgac tcgcttgacg 1321 acgccgttca ggccgccgtg tacgcgcaac aacagttgaa cagcgtcgag ctgcgtcaac 1381 aggtgattaa agccattcgg gtcgctggcg agcgttatgc ccaggtgctg gcggaaatgg 1441 ccgtggccga aaccggcatg ggccgcgtgg tcgataagta catcaaaaac gtgtcacagg 1501 cgcgccatac accagggatc gaatgcctgt cggcggaagt actgaccggt gataatgggc 1561 tgacgctgat tgaaaatgcc ccctggggtg tggttgcgtc ggtcaccccg tcaaccaacc 1621 cggccgcaac cgttatcaat aacgccatca gtatgatagc agccggtaac agcgtggtgt 1681 tcgcccctca tccgtcagcg aaaaacgtat cgctgcgtac catcagcctg ctcaataagg 1741 ccatcgtcgc caccggcggc ccggagaacc tgctggtcag cgtttccgac cccaacatcg 1801 aaaccgcgca acgcctgttt cgctaccccg gtatcgggct gctggttgtc accggcggcg 1861 aagcggtggt ggaagcggcg cgcagacata ccgacaaacg gctgatcgcc gccggagcgg 1921 gtaacccgcc agtggtggtg gatgaaacgg cggatattcc gaaagcagcc cgcgcgattg 1981 ttaaaggcgc ctcattcgac aacaacatta tctgtgccga tgagaaggtg ctgatcgtgg 2041 tggacagtgt ggccgatgcc ctactggcag aaatgcaacg caatcatgcc gtgctgctca 2101 cgccagcaca aaccgaacag ctgctgccag cactgctgag cgacatcgac gagcagggta 2161 aaggtcgcgt caaccgcgac tatgtcggcc gtgatgccac gaaactggct gaagccatcg 2221 ggcttgaggt caacgaatat acccgcttac tgctggctga aaccgacgcc agccacccgt 2281 ttgccgtcac cgagctgatg atgccggtac tacccgtggt gcgggtgaaa agcgtggatg 2341 acgctattgc gctggcactc aaactggaaa atggctgccg ccacaccgcc gcgatgcact 2401 ccaccaatat caggaactta aatcgcatgg ctaacgccat caacaccagt attttcgtca 2461 aaaatggccc ttgtatcgcc gggttggggc tgggtggcga aggttggaca tcgatgacca 2521 tttcgacgcc gaccggtgaa ggtgtcacct ctgctcgcac ctttgtgcgc ctgcgacgct 2581 gtgtgctggt ggatatgttc cgtattgccT aaccgttcat ttatcacaaa aggattgttc 2641 gatGtctatt ccagaaactc aaaaagccat tatcttctac gaatccaacg gcaagttgga 2701 gcataaggat atcccagttc caaagccaaa gcccaacgaa ttgttaatca acgtcaagta 2761 ctctggtgtc tgccacaccg atttgcacgc ttggcatggt gactggccat tgccaactaa 2821 gttaccatta gttggtggtc acgaaggtgc cggtgtcgtt gtcggcatgg gtgaaaacgt 2881 taagggctgg aagatcggtg actacgccgg tatcaaatgg ttgaacggtt cttgtatggc 2941 ctgtgaatac tgtgaattgg gtaacgaatc caactgtcct cacgctgact tgtctggtta 3001 cacccacgac ggttctttcc aagaatacgc taccgctgac gctgttcaag ccgctcacat 3061 tcctcaaggt actgacttgg ctgaagtcgc gccaatcttg tgtgctggta tcaccgtata 3121 caaggctttg aagtctgcca acttgagagc aggccactgg gcggccattt ctggtgctgc 3181 tggtggtcta ggttctttgg ctgttcaata tgctaaggcg atgggttaca gagtcttagg 3241 tattgatggt ggtccaggaa aggaagaatt gtttacctcg ctcggtggtg aagtattcat 3301 cgacttcacc aaagagaagg acattgttag cgcagtcgtt aaggctacca acggcggtgc 3361 ccacggtatc atcaatgttt ccgtttccga agccgctatc gaagcttcta ccagatactg 3421 tagggcgaac ggtactgttg tcttggttgg tttgccagcc ggtgcaaagt gctcctctga 3481 tgtcttcaac cacgttgtca agtctatctc cattgtcggc tcttacgtgg ggaacagagc 3541 tgataccaga gaagccttag atttctttgc cagaggtcta gtcaagtctc caataaaggt 3601 agttggctta tccagtttac cagaaattta cgaaaagatg gagaagggcc aaattgctgg 3661 tagatacgtt gttgacactt ctaaaTgatg ggctgaaggg tttaaGggct gctaacaaag 3721 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg 3781 gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc 3841 ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac 3901 ggtgccTact aagggcgaat gtacagGttt ctacaaactc ttttgtttat ttttctaaat 3961 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg 4021 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc 4081 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga 4141 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga 4201 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg 4261 cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc 4321 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac 4381 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact 4441 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca 4501 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg 4561 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact 4621 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg 4681 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg 4741 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat 4801 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc 4861 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat 4921 actttagatt gatttaaaac ttcattttta at //