LOCUS       CaTCH_empty_back        8059 bp    DNA        circular     24-JUN-2020
DEFINITION  .
ACCESSION   urn.local...1c8-5frjtwu
VERSION     urn.local...1c8-5frjtwu
KEYWORDS    .
SOURCE      
  ORGANISM   .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     ltr             105..287
                     /Description="3' end of full length 3'LTR"
                     /X-Transferred_From="Clipboard Contents 2; Clipboard
                     Contents 6; LUPG-Cas9(PX335)-partial U6;
                     SGEP-GFP-tTA-Linker; VPR sffv;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="Truncated 3'LTR"
                     /label="Truncated 3'LTR"
                     /ApEinfo_label="Truncated 3'LTR"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             1860..2579
                     /locus_tag="GFP"
                     /label="GFP"
                     /ApEinfo_label="GFP"
                     /ApEinfo_fwdcolor="#009c0d"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    105..287
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; Clipboard Contents 6; RIEPTETronly;
                     SGEP-GFP-tTA-Linker; VPR sffv; backup of
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pLVTHM (modified); pLentiCRISPR-P2A-GFP vol.2;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy; pRRL-PX335-P2A-GFP
                     Delta BbsI; pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="5'LTR"
                     /label="5'LTR"
                     /ApEinfo_label="5'LTR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    106..201
                     /gene="R"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; Clipboard Contents 6; SGEP-GFP-tTA-Linker; VPR
                     sffv; pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-PX335-P2A-GFP Delta BbsI"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="R"
                     /label="R"
                     /ApEinfo_label="R"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    204..285
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; Clipboard Contents 6; RIEPTETronly;
                     SGEP-GFP-tTA-Linker; VPR sffv; backup of
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     lentiCas9-Blast (modified);
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pLVTHM (modified); pLentiCRISPR-P2A-GFP vol.2;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy; pRRL-PX335-P2A-GFP
                     Delta BbsI; pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="U5"
                     /label="U5"
                     /ApEinfo_label="U5"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    332..457
                     /note="HIV-1 Psi; packaging signal of human
                     immunodeficiency virus  type 1"
                     /X-Transferred_From="Clipboard Contents 2; Clipboard
                     Contents 6; Exported; SGEP-GFP-tTA-Linker; VPR sffv;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="HIV-1 Psi"
                     /label="HIV-1 Psi"
                     /ApEinfo_label="HIV-1 Psi"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    348..463
                     /gene="HIV-1 pack"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; Clipboard Contents 6; LUPG FKBP tTA P2A;
                     RIEPTETronly; SGEP-GFP-tTA-Linker; VPR sffv; backup of
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pLVTHM (modified); pLentiCRISPR-P2A-GFP vol.2;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy; pRRL-PX335-P2A-GFP
                     Delta BbsI; pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="HIV-1 pack"
                     /label="HIV-1 pack"
                     /ApEinfo_label="HIV-1 pack"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            396..440
                     /X-Transferred_From="Clipboard Contents 2; Clipboard
                     Contents 6; RIEPTETronly; SGEP-GFP-tTA-Linker; VPR sffv;
                     backup of pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pRRL.TETO.SFFV.G (modified); pRRL.TETOrev.SFF;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="HIV-1_psi_pack"
                     /label="HIV-1_psi_pack"
                     /ApEinfo_label="HIV-1_psi_pack"
                     /ApEinfo_fwdcolor="#ff797d"
                     /ApEinfo_revcolor="#ff102f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    396..440
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro;
                     LentiCrisprmCherryP2Apuro-improved tracer; lentiCrispr
                     mCherryP2AneoR-improved tracer; pLV-tTRKRAB (modified);
                     pLentiCRISPR-P2A-Thy1.1; pLentisgRNA Thy1.1-P2A-neo
                     +Kosak"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="RIKPB, pLV-tTRKRAB (modified)"
                     /Transferred_Similarity="100.00%"
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; Clipboard Contents 6;
                     LentiCrisprmCherryP2Apuro;
                     LentiCrisprmCherryP2Apuro-improved tracer; RIEPH;
                     RIEPTETronly; RIKPB; RIKPB Copy; RIKPH;
                     SGEP-GFP-tTA-Linker; VPR sffv; backup of
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     lentiCas9-Blast (modified); lentiCrispr mCherryP2AneoR;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pLV-tTRKRAB (modified); pLVTHM (modified);
                     pLentiCRISPR-P2A-GFP vol.2; pLentiCRISPR-P2A-Thy1.1;
                     pLentisgRNA Thy1.1-P2A-neo +Kosak; pLentisgRNA
                     Thy1.1-P2A-neo +KosakDeltaBbsI Library Vector;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy; pRRL-PX335-P2A-GFP
                     Delta BbsI; pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="HIV-1_psi_pack(1)"
                     /label="HIV-1_psi_pack(1)"
                     /ApEinfo_label="HIV-1_psi_pack"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1409..1442
                     /locus_tag="SBS-12 Seq - Fwd"
                     /label="SBS-12 Seq - Fwd"
                     /ApEinfo_label="SBS-12 Seq - Fwd"
                     /ApEinfo_fwdcolor="#ff8200"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    441..794
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; Clipboard Contents 6; LUPG FKBP tTA P2A;
                     RIEPTETronly; SGEP-GFP-tTA-Linker; VPR sffv; backup of
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pLVTHM (modified); pLentiCRISPR-P2A-GFP vol.2;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy; pRRL-PX335-P2A-GFP
                     Delta BbsI; pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="Gag"
                     /label="Gag"
                     /ApEinfo_label="Gag"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    441..484
                     /gene="dGag'"
                     /SECDrawAs="Region"
                     /X-Transferred_From="Clipboard Contents 2; Clipboard
                     Contents 6; SGEP-GFP-tTA-Linker; VPR sffv;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pRRL.PPT.CBX3.SF; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="dGag' misc"
                     /label="dGag' misc"
                     /ApEinfo_label="dGag' misc"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    485..805
                     /gene="'dGag"
                     /SECDrawAs="Region"
                     /X-Transferred_From="Clipboard Contents 2; Clipboard
                     Contents 6; SGEP-GFP-tTA-Linker; VPR sffv;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pRRL.PPT.CBX3.SF; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="'dGag misc"
                     /label="'dGag misc"
                     /ApEinfo_label="'dGag misc"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    855..870
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; Clipboard Contents 6; RIEPTETronly;
                     SGEP-GFP-tTA-Linker; VPR sffv; backup of
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pLVTHM (modified); pLentiCRISPR-P2A-GFP vol.2;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="SV40_int"
                     /label="SV40_int"
                     /ApEinfo_label="SV40_int"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    950..1183
                     /X-Transferred_Translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRR
                     VVQREKRAVGIGALFLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQ
                     QHLLQLTVWGIKQLQARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLE
                     QIWNHTTWMEWDREINNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNW
                     LWYIKLFIMIVGGLVGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEE
                     GGERDRDRSIRLVNGSLALIWDDLRSLCLFSYHRLRDLLLIVTRIVELLGRRGWEALK
                     YWWNLLQYWSQELKNSAVSLLNATAIAVAEGTDRVIEVVQGACRAIRHIPRRIRQGLE
                     RILL*;
                     MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGALFLGFLGAAGSTMGA
                     ASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARILAVERYLKDQ
                     QLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDREINNYTSLIHSLIEE
                     SQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGLVGLRIVFAVLSIVN
                     RVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVNGSRRYRCRR*;
                     MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGALFLGFLGAAGSTMGA
                     ASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARILAVERYLKDQ
                     QLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDREINNYTSLIHSLIEE
                     SQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGLVGLRIVFAVLSIVN
                     RVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVNGSRRYRDVDDKLCK
                     DG*"
                     /note="RRE; The Rev response element (RRE) of HIV-1 allows
                     for Rev-dependent mRNA export from the nucleus to
                     thecytoplasm."
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; Exported; LUPG FKBP tTA P2A;
                     LUPG-Cas9(PX335)-partial U6; LentiCrisprmCherryP2Apuro;
                     LentiCrisprmCherryP2Apuro-improved tracer; lentiCrispr
                     mCherryP2AneoR; pCMVR8.74 D64V;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pLV-tTRKRAB (modified);
                     pLVTHM (modified); pLentiCRISPR-P2A-GFP vol.2;
                     pLentiCRISPR-P2A-Thy1.1; pLentisgRNA Thy1.1-P2A-neo
                     +Kosak; pLentisgRNA Thy1.1-P2A-neo +KosakDeltaBbsI Library
                     Vector; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="RRE"
                     /label="RRE"
                     /ApEinfo_label="RRE"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1414..1436
                     /locus_tag="1st PCR - Primer-binding site"
                     /label="1st PCR - Primer-binding site"
                     /ApEinfo_label="1st PCR - Primer-binding site"
                     /ApEinfo_fwdcolor="#c3112f"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    961..1165
                     /Description="Rev responsive element; Unknown feature
                     type:Regulatory_Seq"
                     /gene="RRE(HIV) reg"
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pLVTHM
                     (modified); pLentiCRISPR-P2A-GFP vol.2; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="RRE; RRE(HIV) reg"
                     /label="RRE; RRE(HIV) reg"
                     /ApEinfo_label="RRE; RRE(HIV) reg"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2598..2620
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; lentiCas9-Blast (modified);
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pLentiCRISPR-P2A-GFP vol.2"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="HRP"
                     /label="HRP"
                     /ApEinfo_label="HRP"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1414..1432
                     /locus_tag="2nd-primer binding site"
                     /label="2nd-primer binding site"
                     /ApEinfo_label="2nd-primer binding site"
                     /ApEinfo_fwdcolor="#8f0a20"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2622..2745
                     /Description="central polypurine tract (cPPT) and the
                     central termination sequence (CTS)"
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; LUPG-Cas9(PX335)-partial U6;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pLentiCRISPR-P2A-GFP vol.2; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="cPPT; cPPT; cPPT/CTS"
                     /label="cPPT; cPPT; cPPT/CTS"
                     /ApEinfo_label="cPPT; cPPT; cPPT/CTS"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2782..3032
                     /X-Transferred_From="Clipboard Contents 2; lentiCrispr
                     mCherryP2AneoR; pLentiCRISPR-P2A-Thy1.1;
                     pRRL-PX335-P2A-GFP Delta BbsI"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: promoter uukaryotic"
                     /locus_tag="EF1as"
                     /label="EF1as"
                     /ApEinfo_label="EF1as"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     cds             3055..3774
                     /X-Transferred_From="TRE3G-dsRed-miRE (modified)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="TRE3G-dsRed-miRE (modified),
                     pLKO.sgRNA.2stepGibson Reporter"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="eBFP2"
                     /label="eBFP2"
                     /ApEinfo_label="eBFP2"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(3512..3522)
                     /Recognition_pattern="GACNNNNRTGA(12/10)"
                     /annotation_group="GACNNNNRTGA"
                     /X-Transferred_From="gBlock sgRNA test;
                     pLKO.sgRNA.reporter vector"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="gBlock sgRNA test,
                     pLKO.sgRNA.2stepGibson Reporter, pLKO.sgRNA.reporter
                     vector"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="SdeOSI"
                     /label="SdeOSI"
                     /ApEinfo_label="SdeOSI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3541..3551
                     /Recognition_pattern="AAGNNNNNCTT(13/-24)"
                     /annotation_group="AAGNNNNNCTT"
                     /X-Transferred_From="LMN.miRE.Pten.1523;
                     TPGPB-Cas9lentiCRISPR); TRE3G-Cas9-PGK-Blast; gBlock sgRNA
                     test; pLKO.sgRNA.reporter vector"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="gBlock sgRNA test,
                     pLKO.sgRNA.2stepGibson Reporter, pLKO.sgRNA.reporter
                     vector"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="FalI"
                     /label="FalI"
                     /ApEinfo_label="FalI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3561..3571
                     /Recognition_pattern="CCANNNN^NTGG"
                     /annotation_group="CCANNNNNTGG"
                     /X-Transferred_From="gBlock sgRNA test;
                     pLKO.sgRNA.reporter vector"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="gBlock sgRNA test,
                     pLKO.sgRNA.2stepGibson Reporter, pLKO.sgRNA.reporter
                     vector"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="PflMI"
                     /label="PflMI"
                     /ApEinfo_label="PflMI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3791..4366
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; LentiCrisprmCherryP2Apuro;
                     LentiCrisprmCherryP2Apuro-improved tracer; lentiCrispr
                     mCherryP2AneoR; pCRISPR-6xTET-sffv-CAS9-P2A-GFP;
                     pLentiCRISPR-P2A-GFP vol.2; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="WPRE"
                     /label="WPRE"
                     /ApEinfo_label="WPRE"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     cds             complement(4260..4271)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /X-Transferred_Translation="IEGR"
                     /X-Transferred_From="Clipboard Contents 6; Exported;
                     SGEP-GFP-tTA-Linker; VPR sffv;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="Factor Xa recognition and cleavage site CDS"
                     /label="Factor Xa recognition and cleavage site CDS"
                     /ApEinfo_label="Factor Xa recognition and cleavage site
                     CDS"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_signal     4432..4453
                     /SECDrawAs="Label"
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; Clipboard Contents 6; SGEP-GFP-tTA-Linker; VPR
                     sffv; pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="U3PPT"
                     /label="U3PPT"
                     /ApEinfo_label="U3PPT"
                     /ApEinfo_fwdcolor="#008040"
                     /ApEinfo_revcolor="#008040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     ltr             4448..4683
                     /X-Transferred_From="pLentiCRISPR;
                     pLentiCRISPR-P2A-Thy1.1; pLentisgRNA Thy1.1-P2A-neo
                     +Kosak"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="RIKPB"
                     /Transferred_Similarity="100.00%"
                     /X-Transferred_From="Clipboard Contents 2; Clipboard
                     Contents 6; LUPG FKBP tTA P2A; RIEPH; RIEPTETronly; RIKPB;
                     RIKPB Copy; RIKPH; SGEP-GFP-tTA-Linker; VPR sffv; backup
                     of pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pLentiCRISPR; pLentiCRISPR-P2A-Thy1.1;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="3' LTR SIN"
                     /label="3' LTR SIN"
                     /ApEinfo_label="3' LTR SIN"
                     /ApEinfo_fwdcolor="#8882be"
                     /ApEinfo_revcolor="#8882be"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4448..4501
                     /invitrogen=1410000
                     /X-Transferred_From="Clipboard Contents 2; Clipboard
                     Contents 6; RIEPTETronly; SGEP-GFP-tTA-Linker; VPR sffv;
                     backup of pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pHR-SFFV-dCas9-BFP-KRAB (modified);
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="delta U3"
                     /label="delta U3"
                     /ApEinfo_label="delta U3"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            4449..4501
                     /X-Transferred_From="Clipboard Contents 2; Clipboard
                     Contents 6; RIEPTETronly; SGEP-GFP-tTA-Linker; VPR sffv;
                     backup of pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pRRL.TETO.SFFV.G (modified); pRRL.TETOrev.SFF;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="delta_U3"
                     /label="delta_U3"
                     /ApEinfo_label="delta_U3"
                     /ApEinfo_fwdcolor="#ff797d"
                     /ApEinfo_revcolor="#ff102f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4449..4682
                     /gene="SIN"
                     /SECDrawAs="Region"
                     /X-Transferred_From="Clipboard Contents 2; Clipboard
                     Contents 6; SGEP-GFP-tTA-Linker; VPR sffv;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pRRL.PPT.CBX3.SF; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="SIN misc"
                     /label="SIN misc"
                     /ApEinfo_label="SIN misc"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     ltr             4502..4682
                     /note="5' LTR (truncated); truncated 5' long terminal
                     repeat (LTR) from HIV-1"
                     /X-Transferred_From="Clipboard Contents 2; Clipboard
                     Contents 6; Exported; SGEP-GFP-tTA-Linker; VPR sffv;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="5' LTR (truncated)"
                     /label="5' LTR (truncated)"
                     /ApEinfo_label="5' LTR (truncated)"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4502..4684
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; Clipboard Contents 6; RIEPTETronly;
                     SGEP-GFP-tTA-Linker; VPR sffv; backup of
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pLVTHM (modified); pLentiCRISPR-P2A-GFP vol.2;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy; pRRL-PX335-P2A-GFP
                     Delta BbsI; pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="5'LTR(1)"
                     /label="5'LTR(1)"
                     /ApEinfo_label="5'LTR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4502..4683
                     /gene="RU5"
                     /SECDrawAs="Region"
                     /X-Transferred_From="Clipboard Contents 2; Clipboard
                     Contents 6; SGEP-GFP-tTA-Linker; VPR sffv;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pRRL.PPT.CBX3.SF; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="RU5 misc"
                     /label="RU5 misc"
                     /ApEinfo_label="RU5 misc"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4503..4598
                     /gene="R"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; Clipboard Contents 6; SGEP-GFP-tTA-Linker; VPR
                     sffv; pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-PX335-P2A-GFP Delta BbsI"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="R(1)"
                     /label="R(1)"
                     /ApEinfo_label="R"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4599..4683
                     /gene="U5"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; Clipboard Contents 6; LUPG FKBP tTA P2A;
                     RIEPTETronly; SGEP-GFP-tTA-Linker; VPR sffv; backup of
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pLVTHM (modified); pLentiCRISPR-P2A-GFP vol.2;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy; pRRL-PX335-P2A-GFP
                     Delta BbsI; pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="U5(1)"
                     /label="U5(1)"
                     /ApEinfo_label="U5"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4760..4891
                     /X-Transferred_From="Clipboard Contents 2; Clipboard
                     Contents 6; LUPG FKBP tTA P2A; RIEPTETronly;
                     SGEP-GFP-tTA-Linker; VPR sffv; backup of
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pHR-SFFV-dCas9-BFP-KRAB (modified);
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy; pRRL-PX335-P2A-GFP
                     Delta BbsI; pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="SV40 EEL poly(A)s"
                     /label="SV40 EEL poly(A)s"
                     /ApEinfo_label="SV40 EEL poly(A)s"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4851..4870
                     /X-Transferred_Translation="MPSSDDEATADSQHSTPPKKKRKVEDPKDF
                     PSELLSFLSHAVFSNRTLACFAIYTTKEKAALLYKKIMEKYSVTFISRHNSYNHNILF
                     FLTPHRHRVSAINNYAQKLCTFSFLICKGVNKEYLMYSALTRDHNQPYHICRGFTCFK
                     KPPTPPPEPET*"
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro;
                     LentiCrisprmCherryP2Apuro-improved tracer; lentiCrispr
                     mCherryP2AneoR-improved tracer; pLV-tTRKRAB (modified);
                     pLentiCRISPR-P2A-Thy1.1; pLentisgRNA Thy1.1-P2A-neo
                     +Kosak"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_Translation="MPSSDDEATADSQHSTPPKKKRKVEDPKDFPS
                     ELLSFLSHAVFSNRTLACFAIYTTKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFL
                     TPHRHRVSAINNYAQKLCTFSFLICKGVNKEYLMYSALTRDHNQPYHICRGFTCFKKP
                     PTPPPEPET*"
                     /Transferred_From="RIKPB, pLV-tTRKRAB (modified)"
                     /Transferred_Similarity="100.00%"
                     /X-Transferred_Translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIG
                     CSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAG
                     RDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERART
                     RMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFS
                     GFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDE
                     FF*;
                     MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQVGQSKQVDNVFCLLPDGLIPVLIVSR
                     KHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLFHEQLLLLIIFGRPFELFIVAGQIQEI
                     NVFRGQCQLVTFLQLARTSEHSFPAVFKLKERVLGKLNDEVFFDLFISFRLEKVDGVF
                     FEA*;
                     MPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFSNRTLACFAIYTTKE
                     KAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINNYAQKLCTFSFLICK
                     GVNKEYLMYSALTRDHNQPYHICRGFTCFKKPPTPPPEPET*"
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; Clipboard Contents 6; LUPG FKBP tTA P2A;
                     LentiCrisprmCherryP2Apuro;
                     LentiCrisprmCherryP2Apuro-improved tracer; M-ST1-VPR
                     (modified); RIEPH; RIEPTETronly; RIKPB; RIKPB Copy; RIKPH;
                     SGEP-GFP-tTA-Linker; VPR sffv; backup of
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo; lentiCrispr
                     mCherryP2AneoR; pAdSh.PGK.Cas9 (modified);
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pLV-tTRKRAB (modified); pLVTHM (modified);
                     pLentiCRISPR-P2A-GFP vol.2; pLentiCRISPR-P2A-Thy1.1;
                     pLentisgRNA Thy1.1-P2A-neo +Kosak; pLentisgRNA
                     Thy1.1-P2A-neo +KosakDeltaBbsI Library Vector;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="EBV_rev_primer"
                     /label="EBV_rev_primer"
                     /ApEinfo_label="EBV_rev_primer"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4901..4964
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; LUPG FKBP tTA P2A;
                     TRE3G-Cas9+LinkerERT2-P2A-GFP; TRE3G-Cas9GFPERT2;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pLVTHM (modified); pLentiCRISPR-P2A-GFP vol.2;
                     tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="SV40 3x21bp repeat"
                     /label="SV40 3x21bp repeat"
                     /ApEinfo_label="SV40 3x21bp repeat"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      4921..5056
                     /note="SV40 ori; SV40 origin of replication"
                     /X-Transferred_From="Clipboard Contents 2; Clipboard
                     Contents 6; Exported; SGEP-GFP-tTA-Linker; VPR sffv;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="SV40 ori rep origin"
                     /label="SV40 ori rep origin"
                     /ApEinfo_label="SV40 ori rep origin"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5001..5013
                     /Recognition_pattern="GGCCNNNN^NGGCC"
                     /annotation_group="GGCCNNNNNGGCC"
                     /X-Transferred_From="Clipboard Contents 2; Clipboard
                     Contents 6; LUPG FKBP tTA P2A;
                     LentiCrisprmCherryP2Apuro-improved tracer; Puro-P2A-tagBFP
                     reporter; RIEPTETronly; RIKPB; RIKPB Copy; RIKPH;
                     SGEP-GFP-tTA-Linker; TPGPB-Cas9(PX330);
                     TRE3G-Cas9+LinkerERT2-P2A-GFP; TRE3G-Cas9ERT2-P2A-GFP;
                     TRE3G-Cas9GFPERT2; VPR sffv; backup of
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo; pCMVR8.74
                     D64V; pHR-SFFV-dCas9-BFP-KRAB (modified);
                     pLentiCRISPR-P2A-Thy1.1;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy; pRRL-PX335-P2A-GFP
                     Delta BbsI; pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pRRL.SFFV-rtTA3-IRES-EcoR-PGK-Puro (pRRL.RIEP) Copy;
                     pRRL.TETO.SFFV.G (modified); pSIN-reporter with
                     desttagBFP; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="SfiI"
                     /label="SfiI"
                     /ApEinfo_label="SfiI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      5280..5586
                     /X-Transferred_Translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVK
                     VKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQ
                     LGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKEL
                     TAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQ
                     LIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTT
                     GSQATMDERNRQIAEIGASLIKHW*"
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro;
                     LentiCrisprmCherryP2Apuro-improved tracer; lentiCrispr
                     mCherryP2AneoR-improved tracer; pGEM-T vector;
                     pLentiCRISPR-P2A-Thy1.1"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="RIKPB"
                     /Transferred_Similarity="100.00%"
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; Clipboard Contents 6;
                     LentiCrisprmCherryP2Apuro;
                     LentiCrisprmCherryP2Apuro-improved tracer; RIEPH;
                     RIEPTETronly; RIKPB; RIKPB Copy; RIKPH;
                     SGEP-GFP-tTA-Linker; VPR sffv; backup of
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo; lentiCrispr
                     mCherryP2AneoR; pCRISPR-6xTET-sffv-CAS9-P2A-GFP;
                     pLentiCRISPR-P2A-GFP vol.2; pLentiCRISPR-P2A-Thy1.1;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="f1_origin"
                     /label="f1_origin"
                     /ApEinfo_label="f1_origin"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5534..5545
                     /Recognition_pattern="GACNNNN^NNGTC"
                     /annotation_group="GACNNNNNNGTC"
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents 2; Clipboard
                     Contents 6; LUPG FKBP tTA P2A;
                     LentiCrisprmCherryP2Apuro-improved tracer; RIEPTETronly;
                     RIKPB; RIKPB Copy; RIKPH; SGEP-GFP-tTA-Linker; VPR sffv;
                     backup of pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pLentiCRISPR-P2A-Thy1.1;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy; pRRL-PX335-P2A-GFP
                     Delta BbsI; pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="DrdI, AasI, DseDI"
                     /label="DrdI, AasI, DseDI"
                     /ApEinfo_label="DrdI, AasI, DseDI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5713..5724
                     /Recognition_pattern="(11/13)CAANNNNNGTGG(12/10);
                     CAANNNNNGTGG(12/10)"
                     /annotation_group="CAANNNNNGTGG"
                     /X-Transferred_From="Clipboard Contents 2; LUPG FKBP tTA
                     P2A; LentiCrisprmCherryP2Apuro-improved tracer;
                     pLentiCRISPR-P2A-Thy1.1"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="CspCI"
                     /label="CspCI"
                     /ApEinfo_label="CspCI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(5736..5755)
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents 2; Clipboard
                     Contents 6; SGEP-GFP-tTA-Linker; VPR sffv;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="Pinco14"
                     /label="Pinco14"
                     /ApEinfo_label="Pinco14"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5750..5854
                     /invitrogen=2330000
                     /X-Transferred_From="Clipboard Contents 2; Clipboard
                     Contents 6; LMHT-MYCTETR; LMHT-MYCTETRSNLS; RIEPTETronly;
                     SGEP-GFP-tTA-Linker; VPR sffv;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro; pSIN-reporter with
                     desttagBFP; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="bla (Amp) promoter; bla promoter"
                     /label="bla (Amp) promoter; bla promoter"
                     /ApEinfo_label="bla (Amp) promoter; bla promoter"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(6557..6567)
                     /Recognition_pattern="GAYNNNNNVTC(13/8)"
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents 2; Clipboard
                     Contents 6; ES-FUCCI (modified); LMHT-MYCTETR;
                     LMHT-MYCTETRSNLS; LUPG FKBP tTA P2A; RIEPTETronly;
                     SGEP-GFP-tTA-Linker; TPGPB-Cas9lentiCRISPR);
                     TPmCPB-Cas9(lentiCRISPR); TRE3G-Cas9+LinkerERT2-P2A-GFP;
                     TRE3G-Cas9ERT2-P2A-GFP; TRE3G-Cas9GFPERT2; VPR sffv;
                     backup of pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR; pSIN-reporter with
                     desttagBFP; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro;
                     tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="Hin4I"
                     /label="Hin4I"
                     /ApEinfo_label="Hin4I"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(6561..6580)
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents; Clipboard
                     Contents 2; Clipboard Contents 6; ES-FUCCI (modified);
                     LMHT-MYCTETR; LMHT-MYCTETRSNLS; LUPG FKBP tTA P2A;
                     RIEPTETronly; SGEP-GFP-tTA-Linker;
                     TRE3G-Cas9+LinkerERT2-P2A-GFP; TRE3G-Cas9ERT2-P2A-GFP;
                     TRE3G-Cas9GFPERT2; VPR sffv; backup of
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     lentiCas9-Blast (modified); pCR4-TOPO (modified);
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pLVTHM (modified); pLentiCRISPR-P2A-GFP vol.2;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy; pRRL-PX335-P2A-GFP
                     Delta BbsI; pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="Pinco13"
                     /label="Pinco13"
                     /ApEinfo_label="Pinco13"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(6631..6641)
                     /Recognition_pattern="GAYNNNNNVTC(13/8)"
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents 2; Clipboard
                     Contents 6; ES-FUCCI (modified); LMHT-MYCTETR;
                     LMHT-MYCTETRSNLS; LUPG FKBP tTA P2A; RIEPTETronly;
                     SGEP-GFP-tTA-Linker; TPGPB-Cas9lentiCRISPR);
                     TPmCPB-Cas9(lentiCRISPR); TRE3G-Cas9+LinkerERT2-P2A-GFP;
                     TRE3G-Cas9ERT2-P2A-GFP; TRE3G-Cas9GFPERT2; VPR sffv;
                     backup of pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR; pSIN-reporter with
                     desttagBFP; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro;
                     tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="Hin4I(1)"
                     /label="Hin4I(1)"
                     /ApEinfo_label="Hin4I"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(6838..6849)
                     /Recognition_pattern="GACNNNNNNTGG(12/7)"
                     /annotation_group="GACNNNNNNTGG"
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents 2; Clipboard
                     Contents 6; ES-FUCCI (modified); LMHT-MYCTETR;
                     LMHT-MYCTETRSNLS; LUPG FKBP tTA P2A; RIEPTETronly;
                     SGEP-GFP-tTA-Linker; TPGPB-Cas9lentiCRISPR);
                     TPmCPB-Cas9(lentiCRISPR); TRE3G-Cas9+LinkerERT2-P2A-GFP;
                     TRE3G-Cas9ERT2-P2A-GFP; TRE3G-Cas9GFPERT2; VPR sffv;
                     backup of pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCR4-TOPO (modified); pCRISPR-6xTET-sffv-CAS9-P2A-GFP;
                     pHR-SFFV-dCas9-BFP-KRAB (modified);
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR; pSIN-reporter with
                     desttagBFP; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro;
                     tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="Bsp24I"
                     /label="Bsp24I"
                     /ApEinfo_label="Bsp24I"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6854..7527
                     /Description="3 extra bp in the middle, compared to pLVX
                     ori - ACAAAAAAACC(ACC)GCTACCAG"
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; LUPG-Cas9(PX335)-partial U6;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pLentiCRISPR-P2A-GFP
                     vol.2; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="pUC ori"
                     /label="pUC ori"
                     /ApEinfo_label="pUC ori"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      6864..7483
                     /X-Transferred_Translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVK
                     VKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQ
                     LGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKEL
                     TAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQ
                     LIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTT
                     GSQATMDERNRQIAEIGASLIKHW*"
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; LentiCrisprmCherryP2Apuro;
                     LentiCrisprmCherryP2Apuro-improved tracer; lentiCrispr
                     mCherryP2AneoR; pCMVR8.74 D64V;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pLentiCRISPR-P2A-Thy1.1;
                     tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="pBR322_origin"
                     /label="pBR322_origin"
                     /ApEinfo_label="pBR322_origin"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      7301..7521
                     /Description="putative"
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents 2; Clipboard
                     Contents 6; VPR sffv; pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="PMB1"
                     /label="PMB1"
                     /ApEinfo_label="PMB1"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    7666..7676
                     /Recognition_pattern="ACNNNNNCTCC(10/7)"
                     /annotation_group="ACNNNNNCTCC"
                     /X-Transferred_From="Clipboard Contents 2; Clipboard
                     Contents 6; LMHT-MYCTETR; LMHT-MYCTETRSNLS; RIEPTETronly;
                     SGEP-GFP-tTA-Linker; VPR sffv; pCR4-TOPO (modified);
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="BsaXI"
                     /label="BsaXI"
                     /ApEinfo_label="BsaXI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    7756..7777
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP.; CAP binding site"
                     /X-Transferred_From="Clipboard Contents 2; Clipboard
                     Contents 6; Exported; SGEP-GFP-tTA-Linker; VPR sffv;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="CAP binding site protein bind"
                     /label="CAP binding site protein bind"
                     /ApEinfo_label="CAP binding site protein bind"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    join(7933..8059,1..286)
                     /X-Transferred_From="Clipboard Contents 2;
                     LentiCrisprmCherryP2Apuro;
                     LentiCrisprmCherryP2Apuro-improved tracer; lentiCrispr
                     mCherryP2AneoR; pLentiCRISPR-P2A-Thy1.1; pLentisgRNA
                     Thy1.1-P2A-neo +Kosak; pLentisgRNA Thy1.1-P2A-neo
                     +KosakDeltaBbsI Library Vector; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="5'LTR(2)"
                     /label="5'LTR(2)"
                     /ApEinfo_label="5'LTR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        join(7935..8059,1..104)
                     /note="RSV promoter; Rous sarcoma virus enhancer/promoter"
                     /X-Transferred_From="Exported"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="RSV promoter"
                     /label="RSV promoter"
                     /ApEinfo_label="RSV promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(8037..8048)
                     /Recognition_pattern="CACNNNNNNTCC(12/7)"
                     /annotation_group="CACNNNNNNTCC"
                     /X-Transferred_From="Clipboard Contents 2; Clipboard
                     Contents 6; LUPG FKBP tTA P2A; RIEPTETronly; RIKPB; RIKPB
                     Copy; RIKPH; SGEP-GFP-tTA-Linker; VPR sffv; backup of
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pLentiCRISPR-P2A-Thy1.1;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy; pRRL-PX335-P2A-GFP
                     Delta BbsI; pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pRRL.TETO.SFFV.G (modified);
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="TstI"
                     /label="TstI"
                     /ApEinfo_label="TstI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1704..1707
                     /locus_tag="BC-cloning-overhang"
                     /label="BC-cloning-overhang"
                     /ApEinfo_label="BC-cloning-overhang"
                     /ApEinfo_fwdcolor="#fea700"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1674..1707
                     /locus_tag="BC-cloning-site"
                     /label="BC-cloning-site"
                     /ApEinfo_label="BC-cloning-site"
                     /ApEinfo_fwdcolor="#e4e9e9"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1674..1677
                     /locus_tag="BC-cloning-overhang(1)"
                     /label="BC-cloning-overhang(1)"
                     /ApEinfo_label="BC-cloning-overhang"
                     /ApEinfo_fwdcolor="#ffa800"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1679..1684
                     /locus_tag="BsmBI"
                     /label="BsmBI"
                     /ApEinfo_label="BsmBI"
                     /ApEinfo_fwdcolor="#00ff0a"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1697..1702
                     /locus_tag="BsmBI(1)"
                     /label="BsmBI(1)"
                     /ApEinfo_label="BsmBI"
                     /ApEinfo_fwdcolor="#00ff26"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1618..1637
                     /locus_tag="GBLOCK-overhang"
                     /label="GBLOCK-overhang"
                     /ApEinfo_label="GBLOCK-overhang"
                     /ApEinfo_fwdcolor="#ffe46e"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1385..1408
                     /locus_tag="P7-Fwd"
                     /label="P7-Fwd"
                     /ApEinfo_label="P7-Fwd"
                     /ApEinfo_fwdcolor="#fffc00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1854..1859
                     /locus_tag="Kozak"
                     /label="Kozak"
                     /ApEinfo_label="Kozak"
                     /ApEinfo_fwdcolor="#9becff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        1719..1842
                     /vntifkey=29
                     /locus_tag="From TRE3G (contains mCMV)"
                     /label="From TRE3G (contains mCMV)"
                     /ApEinfo_label="From TRE3G (contains mCMV)"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1704..1723
                     /locus_tag="1st-PCR reverse-primer"
                     /label="1st-PCR reverse-primer"
                     /ApEinfo_label="1st-PCR reverse-primer"
                     /ApEinfo_fwdcolor="#ff8200"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1704..1706
                     /locus_tag="PAM"
                     /label="PAM"
                     /ApEinfo_label="PAM"
                     /ApEinfo_fwdcolor="#d204ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc
       61 attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag
      121 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
      181 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
      241 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
      301 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
      361 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
      421 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
      481 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
      541 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
      601 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
      661 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
      721 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
      781 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
      841 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
      901 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
      961 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     1021 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     1081 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     1141 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     1201 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     1261 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     1321 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     1381 aattCAAGCA GAAGACGGCA TACGAGATGT GACTGGAGTT CAGACGTGTG CTCTTCCGAT
     1441 CTattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     1501 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     1561 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     1621 cacctcccaa ccccgagggg accctatcat atgctgggta ccctgtctag agcGAACgga
     1681 gacgactagt gatatccgtc tctcggcagg agaagcttta ggcgtgtacg gtgggcgcct
     1741 ataaaagcag agctcgttta gtgaaccgtc agatcgcctg gagcaattcc acaacacttt
     1801 tgtcttatac caactttccg taccacttcc taccctcgta aactggagga tccgccacca
     1861 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     1921 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     1981 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     2041 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
     2101 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     2161 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     2221 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     2281 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     2341 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     2401 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     2461 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     2521 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaag
     2581 aattctagat cttgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     2641 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     2701 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     2761 gagatccact ttggcgccgg ctcgagtggc tccggtgccc gtcagtgggc agagcgcaca
     2821 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga
     2881 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag
     2941 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg
     3001 tttgccgcca gaacacaggt gtcgtgacgc ggatcgtacc ccgaatttat gatcatggtg
     3061 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     3121 gtaaacggcc acaagttcag cgtgaggggc gagggcgagg gcgatgccac caacggcaag
     3181 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     3241 accaccctga gccacggcgt gcagtgcttc gcccgctacc ccgaccacat gaagcagcac
     3301 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     3361 gacgacggca cctacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     3421 cgcatcgagc tgaagggcgt cgacttcaag gaggacggca acatcctggg gcacaagctg
     3481 gagtacaact tcaacagcca caacatctat atcatggccg tcaagcagaa gaacggcatc
     3541 aaggtgaact tcaagatccg ccacaacgtg gaggacggca gcgtgcagct cgccgaccac
     3601 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacag ccactacctg
     3661 agcacccagt ccgtgctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     3721 gagttccgca ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaacgcgtt
     3781 aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     3841 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     3901 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     3961 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     4021 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     4081 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     4141 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     4201 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     4261 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     4321 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcgtc
     4381 gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     4441 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
     4501 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     4561 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     4621 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     4681 cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     4741 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     4801 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     4861 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     4921 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     4981 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     5041 ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc
     5101 gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     5161 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     5221 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg
     5281 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     5341 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     5401 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     5461 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     5521 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     5581 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     5641 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     5701 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     5761 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     5821 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     5881 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     5941 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     6001 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     6061 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     6121 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     6181 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     6241 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     6301 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     6361 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     6421 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     6481 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     6541 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     6601 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     6661 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     6721 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     6781 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     6841 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     6901 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     6961 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     7021 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     7081 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     7141 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     7201 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     7261 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     7321 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     7381 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     7441 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     7501 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     7561 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     7621 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     7681 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     7741 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     7801 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     7861 cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct
     7921 ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg
     7981 atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa
     8041 gtaaggtggt acgatcgtg    
//
