LOCUS       Exported                5588 bp ds-DNA     circular SYN 30-JUN-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5588)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jun 30, 2020 from SnapGene Viewer 5.1.4.1
            https://www.snapgene.com
COMMENT     [DEFINITION]: .
[LOCUS]: AurkA_Dnter
[ACCESSION]:
FEATURES             Location/Qualifiers
     source          1..5588
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1
                     /label=New Feature
     rep_origin      1005..1633
                     /direction=RIGHT
                     /label=ColE1 origin
     misc_feature    1585..1592
                     /label=ORF
                     /note="label=ORF - ApEinfo_fwdcolor=#ff8040 - 
                     ApEinfo_revcolor=green - ApEinfo_graphicformat=arrow_data 
                     {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 1585..1592"
     misc_feature    1740..1964
                     /label=CMV
                     /note="label=CMV - ApEinfo_fwdcolor=#00b900 - 
                     ApEinfo_revcolor=green - ApEinfo_graphicformat=arrow_data 
                     {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 1740..1964"
     misc_feature    1965..2147
                     /label=CMV(1)
                     /note="label=CMV(1) - ApEinfo_label=CMV - 
                     ApEinfo_fwdcolor=#00b900 - ApEinfo_revcolor=green - 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} - 
                     width 5 offset 0 - 1965..2147"
     misc_feature    2080..2100
                     /label=pCMV F
     misc_feature    2183..3241
                     /label=AurkA
                     /note="label=AurkA - ApEinfo_fwdcolor=#5e5eff - 
                     ApEinfo_revcolor=green - ApEinfo_graphicformat=arrow_data 
                     {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 2183..3241"
     misc_feature    complement(2232..2251)
                     
     misc_feature    complement(2916..2935)
                     /label=AURKA K162M R
     CDS             2933..2944
                     /label=FactorXa site
     misc_feature    3263..3979
                     /label=GFP
                     /note="label=GFP - ApEinfo_fwdcolor=cyan - 
                     ApEinfo_revcolor=green - ApEinfo_graphicformat=arrow_data 
                     {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 3263..3979"
     CDS             3266..3979
                     /label=EGFP
     misc_feature    complement(3308..3324)
                     /label=pEGFP_N R
     misc_feature    3921..3939
                     /label=pEGFP_C F
     misc_feature    complement(4027..4046)
                     /label=pEGFP_Rev
     rep_origin      4243..4683
                     /direction=RIGHT
                     /label=F1 ori
     promoter        4750..4778
                     /label=Amp prom
     promoter        4982..5178
                     /label=SV40 early promoter
     misc_feature    5029..5106
                     /label=SV40 ORI
     CDS             join(5216..5588,1..419)
                     /label=Kan/neoR
ORIGIN
        1 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
       61 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
      121 cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg
      181 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
      241 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
      301 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
      361 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
      421 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
      481 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
      541 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag
      601 gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa
      661 aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca
      721 gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc
      781 ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt
      841 cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa
      901 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
      961 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     1021 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     1081 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     1141 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     1201 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     1261 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     1321 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     1381 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     1441 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     1501 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     1561 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     1621 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     1681 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gccatgcatt agttattaat
     1741 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     1801 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     1861 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     1921 atttacggta aactgcccac ttggcagtac atcaagtgta tcatcggttt gactcacggg
     1981 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
     2041 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
     2101 tacggtggga ggtctatata agcagagctg gtttagtgaa ccgtcagatc cgctagcgct
     2161 accggactca gatctcgaga tgtcaaattc ttcccagcgc attcctttgc aagcacaaaa
     2221 acttgtctcc agtcacaagc cggttcagaa tcagaagcag aagcaattgc aggcaaccag
     2281 tgtacctcat cctgtctcca ggccactgaa taacacccaa aagagcaagc agcccctgcc
     2341 atcggcacct gaaaataatc ctgaggagga actggcatca aaacagaaaa atgaagaatc
     2401 aaaaaagagg cagtgggctt tggaagactt tgaaattggt cgccctctgg gtaaaggaaa
     2461 gtttggtaat gtttatttgg caagagaaaa gcaaagcaag tttattctgg ctcttaaagt
     2521 gttatttaaa gctcagctgg agaaagccgg agtggagcat cagctcagaa gagaagtaga
     2581 aatacagtcc caccttcggc atcctaatat tcttagactg tatggttatt tccatgatgc
     2641 taccagagtc tacctaattc tggaatatgc accacttgga acagtttata gagaacttca
     2701 gaaactttca aagtttgatg agcagagaac tgctacttat ataacagaat tggcaaatgc
     2761 actatcatat tgccattcga agagagttat tcatagagac attaagccag agaacttact
     2821 tcttggatca gctggagagc ttaaaattgc agattttggg tggtcagtac atgctccatc
     2881 ttccaggagg accactctct gtggcaccct ggactacctg ccccctgaaa tgattgaagg
     2941 tcggatgcat gatgagaagg tggatctctg gagccttgga gttctttgct atgaattttt
     3001 agttgggaag cctccttttg aggcaaacac ataccaagag acctacaaaa gaatatcacg
     3061 ggttgagttc acattccctg actttgtaac agagggagcc agggacctca tttcaagact
     3121 gttgaagcat aatcccagcc agaggccaat gctcagagaa gtacttgaac acccctggat
     3181 cacagcaaat tcatcaaaac catcaaattg ccaaaacaaa gaatcagcta gcaaacagtc
     3241 tggggatcca ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
     3301 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     3361 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     3421 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     3481 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     3541 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     3601 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     3661 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     3721 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     3781 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     3841 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     3901 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     3961 catggacgag ctgtacaagt aaagcggccg cgactctaga tcataatcag ccataccaca
     4021 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     4081 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     4141 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     4201 ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata ttttgttaaa
     4261 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     4321 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     4381 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     4441 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     4501 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     4561 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     4621 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     4681 gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     4741 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     4801 atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta
     4861 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     4921 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     4981 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     5041 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     5101 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     5161 ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga
     5221 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     5281 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     5341 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga
     5401 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     5461 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     5521 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     5581 gcatacgc
//
