LOCUS       Exported                5129 bp ds-DNA     circular SYN 30-JUN-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5129)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jun 30, 2020 from SnapGene Viewer 5.1.4.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5129
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      996..1624
                     /direction=RIGHT
                     /label=ColE1 origin
     misc_feature    1731..2306
                     /label=pCMV
     misc_feature    2348..2782
                     /label=Tom20
     CDS             2807..3511
                     /label=mCherry
     rep_origin      3775..4215
                     /direction=RIGHT
                     /label=F1 ori
     promoter        4282..4310
                     /label=Amp prom
     promoter        4514..4710
                     /label=SV40 early promoter
     misc_feature    4561..4638
                     /label=SV40 ORI
     CDS             join(4748..5129,1..410)
                     /label=Kan/neoR
ORIGIN
        1 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
       61 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
      121 aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg
      181 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
      241 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
      301 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
      361 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
      421 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
      481 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
      541 cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga
      601 aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa
      661 taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca
      721 ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc
      781 cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc
      841 aggccctgcc atagcctcag gttactcata tatactttag attgatttaa aacttcattt
      901 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
      961 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     1021 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     1081 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     1141 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     1201 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     1261 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     1321 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     1381 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     1441 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     1501 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     1561 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     1621 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     1681 atcccctgat tctgtggata accgtattac cgccatgcat tagttattaa tagtaatcaa
     1741 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     1801 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     1861 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     1921 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     1981 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2041 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2101 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2161 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2221 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2281 gcagagctgg tttagtgaac cgtcagatcc gctagcgcta ccggactcag atctcgagct
     2341 caagcttatg gtgggtcgga acagcgccat cgccgccggt gtatgcgggg cccttttcat
     2401 tgggtactgc atctacttcg accgcaaaag acgaagtgac cccaacttca agaacaggct
     2461 tcgagaacga agaaagaaac agaagcttgc caaggagaga gctgggcttt ccaagttacc
     2521 tgaccttaaa gatgctgaag ctgttcagaa gttcttcctt gaagaaatac agcttggtga
     2581 agagttacta gctcaaggtg aatatgagaa gggcgtagac catctgacaa atgcaattgc
     2641 tgtgtgtgga cagccacagc agttactgca ggtcttacag caaactcttc caccaccagt
     2701 gttccagatg cttctgacta agctcccaac aattagtcag agaattgtaa gtgctcagag
     2761 cttggctgaa gatgatgtgg aaggggatcc accggtcgcc accatggtga gcaagggcga
     2821 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
     2881 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     2941 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
     3001 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
     3061 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     3121 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
     3181 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
     3241 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
     3301 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
     3361 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
     3421 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
     3481 ctccaccggc ggcatggacg agctgtacaa gtaaagcggc cgcgactcta gatcataatc
     3541 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
     3601 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
     3661 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     3721 tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtaaattg taagcgttaa
     3781 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     3841 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     3901 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     3961 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     4021 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     4081 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     4141 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     4201 tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     4261 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     4321 aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat
     4381 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     4441 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     4501 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     4561 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     4621 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     4681 ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt
     4741 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     4801 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     4861 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     4921 actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     4981 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     5041 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     5101 aatgcggcgg ctgcatacgc ttgatccgg
//
